scholarly journals Studies on the therapeutic effects of three TCM methods on steroid-induced osteonecrosis of femoral head

2020 ◽  
Vol 19 (8) ◽  
pp. 1701-1708
Author(s):  
Xuyi Tan ◽  
Xiantao Chen ◽  
Xiaobo Duan ◽  
Shutu Gao ◽  
Yuxin Guo ◽  
...  
Keyword(s):  
Femoral Head ◽  
Total Cholesterol ◽  
Bone Marrow Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
Rabbit Model ◽  
Serum Total Cholesterol ◽  
Therapeutic Effects ◽  
Mrna Levels ◽  
Osteonecrosis Of Femoral Head ◽  
Linggui Zhugan Decoction

Purpose: To determine the therapeutic effects of kidney yang-tonifying, blood-activating and phlegmeliminating methods on steroid-induced osteonecrosis of femoral head (SONFH), and their mechanisms of action.Methods: A rabbit model of SONFH was established. Six rabbit groups were used: control, SONFH (given normal saline); tongluo shenggu group, kidney-tonifying group, blood-activating group, and phlegm--eliminating group, which received tongluo shenggu capsule (0.28 g/kg/day), yougui yin (YGY, 2.52 g/kg/day), taohong siwu decoction (TSD, 2.38 g/kg/day) and linggui zhugan decoction (LZD,1.54 g/kg/day), respectively, for 8 weeks. The following serum indices were assayed on the 2nd, 4th and 8th weeks: total cholesterol, triglycerides, thromboxane B2 (TXB2), 6-keto-PGF1α, osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-Β ligand (RANKL). At the end of the 8th week, mRNA levels of OPG, BMP-2 and VEGF were assayed using real-time fluorescent quantitative polymerase chain reaction (RTF-qPCR).Results: Serum OPG in the kidney-tonifying group was increased, while its RANKL was decreased on the 2nd week, but these parameters, along with mRNA of OPG and BMP-2, reached normal levels at the 8th week (p <0.05). The TXB2 of blood-activating group was decreased, while its 6-keto-PGF1 level was increased at the 2nd week, but both of them attained normal levels at the 8th week, along with VEGF mRNA. Serum total cholesterol, triglycerides and TXB2 in the phlegm-eliminating group were decreased, while its 6-keto-PGF1α was increased at the 2nd week, but the levels of serum total cholesterol and triglycerides were normalized at the 8th week (p <0.05). Histological examination showed increases in bone trabeculae and bone marrow cells, while the proliferation of fat cells was decreased in kidney-tonifying, blood-activating and phlegm-eliminating groups.Conclusion: These findings suggest that the therapeutic effects of the three different TCM methods on SONFH involve different mechanisms. Keywords: Steroid-induced osteonecrosis, Femoral head, Yougui yin, Taohong siwu, Linggui zhugan decoction, Kidney-tonifying yang, Phlegm

scholarly journals Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet

2006 ◽  
Vol 95 (5) ◽  
pp. 916-924 ◽  
Author(s):  
Takashi Fushimi ◽  
Kazuhito Suruga ◽  
Yoshifumi Oshima ◽  
Momoko Fukiharu ◽  
Yoshinori Tsukamoto ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Bile Acid ◽  
Total Cholesterol ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Mrna Level ◽  
Serum Total Cholesterol ◽  
Free Access ◽  
Mrna Levels ◽  
Atp Citrate Lyase

To investigate the efficacy of the intake of vinegar for prevention of hyperlipidaemia, we examined the effect of dietary acetic acid, the main component of vinegar, on serum lipid values in rats fed a diet containing 1% (w/w) cholesterol. Animals were allowed free access to a diet containing no cholesterol, a diet containing 1% cholesterol without acetic acid, or a diet containing 1% cholesterol with 0·3% (w/w) acetic acid for 19 d. Then, they were killed after food deprivation for 7 h. Cholesterol feeding increased serum total cholesterol and triacylglycerol levels. Compared with the cholesterol-fed group, the cholesterol and acetic acid-fed group had significantly lower values for serum total cholesterol and triacylglycerols, liver ATP citrate lyase (ATP-CL) activity, and liver 3-hydroxy-3-methylglutaryl-CoA content as well as liver mRNA levels of sterol regulatory element binding protein-1, ATP-CL and fatty acid synthase (P<,0·05). Further, the serum secretin level, liver acyl-CoA oxidase expression, and faecal bile acid content were significantly higher in the cholesterol and acetic acid-fed group than in the cholesterol-fed group (P<0·05). However, acetic acid feeding affected neither the mRNA level nor activity of cholesterol 7a-hydroxylase. In conclusion, dietary acetic acid reduced serum total cholesterol and triacylglycerol: first due to the inhibition of lipogenesis in liver; second due to the increment in faecal bile acid excretion in rats fed a diet containing cholesterol.

scholarly journals Ghrelin attenuates avascular necrosis of the femoral head induced by steroids in rabbits

2020 ◽  
Vol 19 (10) ◽  
pp. 2097-2101
Author(s):  
Laigang Huang ◽  
Fanshuo Zeng ◽  
Baojuan Cui ◽  
Daoqing Wang ◽  
Min Sun ◽  
...  
Keyword(s):  
Femoral Head ◽  
Avascular Necrosis ◽  
Rabbit Model ◽  
Underlying Mechanism ◽  
Bone Morphogenetic Protein 2 ◽  
Vehicle Group ◽  
Control Group ◽  
Mrna Levels ◽  
Growth Hormone Secretagogue Receptor ◽  
Growth Hormone Secretagogue

Purpose: Ghrelin is an endogenous ligand for growth hormone secretagogue receptor. The current study was aimed at examining the effect of ghrelin on avascular necrosis of the femoral head (ANFH) induced by steroids in a rabbit model and also exploring the underlying mechanism. Methods: Experimental rabbits were separated into three groups: Control, Vehicle and Ghrelin. We established a steroid-induced ANFH model in rabbits. Then, MRI scanning and hematoxylin-eosin staining (HE) were conducted to see ANFH. The mRNA levels of Vascular Endothelial Growth Factor (VEGF) and Bone Morphogenetic Protein 2 (BMP-2) were evaluated using real-time qRT-PCR. Results: Rabbits in the Vehicle group showed increased empty bone lacunae, reduced bone trabecula in femoral head; the number of hematopoietic cells in the bone marrow was reduced, whereas number of adipocytes increased with evident fusion phenomenon in comparison with the Control group. All of the changes induced in Vehicle group were attenuated in Ghrelin group. MRI scanning showed obvious necrosis of femoral head in the Vehicle group and less in the Ghrelin group. The mRNA levels of VEGF and BMP-2 were raised in Vehicle group and further enhanced in Ghrelin group. Conclusion: Ghrelin attenuates steroid-induced avascular necrosis in femoral head in rabbit model. A possible mechanism may be through VEGF/BMP-2 axis. Keywords: ANFH, BMP-2, Ghrelin, VEGF

HRA Model for Hypercholesterolemia Based on a Longitudinal Health Database

1998 ◽  
Vol 37 (02) ◽  
pp. 130-133
Author(s):  
T. Kishimoto ◽  
Y. Iida ◽  
K. Yoshida ◽  
M. Miyakawa ◽  
H. Sugimori ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Proportional Hazards ◽  
Proportional Hazards Model ◽  
Model Analysis ◽  
Serum Total Cholesterol ◽  
Hazards Model ◽  
The Mean ◽  
Health Database ◽  
Observation Period

AbstractTo evaluate the risk factors for hypercholesterolemia, we examined 4,371 subjects (3,207 males and 1,164 females) who received medical checkups more than twice at an AMHTS in Tokyo during the period from 1976 through 1991; and whose serum total cholesterol was under 250 mg/dl. The mean follow-up duration was 6.6 years. A self-registering questionnaire was administered at the time of the health checkup. The endpoint of this study was the onset of hypercholesterolemia when the level of serum total cholesterol was 250 mg/dl and over. We compared two prognosis groups (normal and hypercholesterol) in terms of age, examination findings and lifestyle. After assessing each variable, we employed Cox's proportional hazards model analysis to determine the factors related to the occurrence of hypercholesterolemia. According to proportional hazards model analysis, total cholesterol, triglyceride and smoking at the beginning, and hypertension during the observation period were selected in males; and total cholesterol at the beginning and age were selected in females to determine the factors related to the occurrence of hypercholesterolemia.

Operated unstable pertrochanteric fractures leading to osteonecrosis of femoral head

2015 ◽  
Vol 2 (2) ◽  
pp. 113-116
Author(s):  
Neetin P. Mahajan ◽  
Ankit A. Marfatia ◽  
Nadir Z. Shah ◽  
F Memon ◽  
S. Mukherjee ◽  
...  
Keyword(s):  
Femoral Head ◽  
Osteonecrosis Of Femoral Head ◽  
Pertrochanteric Fractures

scholarly journals Rapid ultramicro estimation of serum total cholesterol

1960 ◽  
Vol 1 (4) ◽  
pp. 349-351
Author(s):  
Ronald L. Searcy ◽  
Lois M. Bergquist ◽  
Ralph C. Jung
Keyword(s):  
Total Cholesterol ◽  
Serum Total Cholesterol

CaO2/Gelatin Oxygen Slow-Releasing Microspheres Facilitate Tissue Engineering Efficiency for Osteonecrosis of Femoral Head via Enhancing Angiogenesis and Survival of Grafted Bone Marrow Mesenchyml Stem Cells

2021 ◽  
Author(s):  
Chengqiang Wang ◽  
Haixia Xu ◽  
Chun Liu ◽  
Ziyue Peng ◽  
Ruoxing Min ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Femoral Head ◽  
Refractory Disease ◽  
Pathogenic Factor ◽  
Osteonecrosis Of Femoral Head ◽  
Critical Challenge

Osteonecrosis of femoral head (ONFH), a common refractory disease, is not fully understood today. Hypoxia caused by ischemia is not only an important pathogenic factor, but also a critical challenge...

scholarly journals Simple cardiovascular risk stratification using anthropometric measures instead of serum cholesterol. The MORGAM Prospective Cohort Project

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
A Kristensen ◽  
V Rosberg ◽  
J Vishram-Nielsen ◽  
M Pareek ◽  
A Linneberg ◽  
...  
Keyword(s):  
Cardiovascular Risk ◽  
Risk Stratification ◽  
Total Cholesterol ◽  
Serum Cholesterol ◽  
Cardiovascular Events ◽  
Smoking Status ◽  
Predictive Ability ◽  
Serum Total Cholesterol ◽  
Anthropometric Measures ◽  
Blood Pressures

Abstract Background Body composition predicts cardiovascular outcomes, but it is uncertain whether anthropometric measures can replace the more expensive serum total cholesterol for cardiovascular risk stratification in low resource settings. Purpose The purpose of the study was to compare the additive prognostic ability of serum total cholesterol with that of body mass index (BMI), waist/hip ratio (WHR), and estimated fat mass (EFM, calculated using a validated prediction equation), individually and combined. Methods We used data from the MORGAM (MONICA, Risk, Genetics, Archiving, and Monograph) Prospective Cohort Project, an international pooling of cardiovascular cohorts, to determine the relationship between anthropometric measures, serum cholesterol, and cardiovascular events, using multivariable Cox proportional-hazards regression analysis. We further investigated the ability of these measures to enhance prognostication beyond a simpler prediction model, consisting of age, sex, smoking status, systolic blood pressures, and country, using comparison of area under the receiver operating characteristics curve (AUCROC) derived from binary logistic regression models. The primary endpoint was major adverse cardiovascular events (MACE), defined as a composite of death from coronary heart disease, myocardial infarction, or stroke. Results The study population consisted of 52,188 apparently healthy subjects (56.3% men) aged 47±12 years ranging from 20 to 84, derived from 37 European cohorts, with baseline between 1982–2002 all followed for 10 years during which MACE occurred in 2465 (4.7%) subjects. All anthropometric measures (BMI: hazard ratio (HR) 1.04 [95% confidence interval (CI): 1.03–1.05] per kg/m2; WHR: HR 7.5 [4.0–14.0] per unit; EFM: HR 1.02 [1.01–1.02] per kg) as well as serum total cholesterol (HR 1.20 [1.16–1.24] per mmol/l) were significantly associated with MACE (P&lt;0.001 for all), independently of age, sex, smoking status, systolic blood pressures, and country. The addition of serum cholesterol significantly improved the predictive ability of the simple model (AUCROC 0.818 vs. 0.814, P&lt;0.001), as did the combination of WHR, BMI, and EFM (AUCROC 0.817 vs. 0.814, P=0.004). When assessed individually, BMI (AUCROC 0.816 vs. 0.814, P=0.004) and WHR (AUCROC 0.815 vs. 0.814, P=0.02) improved model performance, while EFM narrowly missed significance (AUCROC 0.815 vs. 0.814, P=0.06). There was no significant difference in the predictive ability of a model including serum cholesterol versus that including all three anthropometric measures (AUCROC 0.818 vs. 0.817, P=0.13). The figure shows the pertinent areas under the ROC curve in predicting MACE. Conclusion In this large population-based cohort study, the addition of a combination of anthropometric measures, i.e. BMI, WHR, and EFM, raised the predictive ability of a simple prognostic model comparable to that obtained by the addition of serum total cholesterol. Figure 1 Funding Acknowledgement Type of funding source: None

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