Microbiological Comparison of Hot-Boned and Conventionally Processed Beef Plate Cuts During Extended Storage1

1982 ◽  
Vol 45 (7) ◽  
pp. 607-614 ◽  
Author(s):  
J. E. KENNEDY ◽  
J. L. OBLINGER ◽  
R. L. WEST
Keyword(s):  
Yersinia Enterocolitica ◽  
Processing Technique ◽  
Post Mortem ◽  
Microbial Counts ◽  
Processing Treatment ◽  
Brochothrix Thermosphacta ◽  
Taxonomic Characterization ◽  
Lactobacillus Spp ◽  
Microbial Isolates

The development of microflora on hot-boned and conventionally processed beef plate cuts was investigated from time of slaughter and/or fabrication throughout vacuum-packaged storage for 6 weeks at 0–1 C. Cuts from each processing treatment were analyzed immediately post-mortem and after 0.5, 1, 1.5, 2, 4, 7, 14, 21, 28 and 42 days of storage. Fabrication, packaging and chilling of beef plates were carefully controlled to minimize differences in chilling rates and contamination of hot and conventionally processed cuts. Microbial analyses included enumeration of mesophilic, psychrotrophic and total Enterobacteriaceae populations as well as taxonomic characterization of corresponding microbial isolates. Microbial counts of hot-boned cuts were generally higher than corresponding counts of conventionally processed cuts with significant differences (p<0.05) detected between mesophilic and psychrotrophic counts at most storage intervals between 14 and 42 days. Earlier predominance of organisms such as Lactobacillus spp. and Brochothrix thermosphacta on hot-boned vs. conventionally processed cuts was indicated by taxonomic determinations. Psychrotrophic Enterobacteriaceae, including Hafnia alvei and Yersinia enterocolitica-like organisms, were recovered in high numbers from a few samples after 28 and 42 days of storage regardless of processing technique. Differences in the development of microbial flora on hot and conventionally processed beef cuts could not be explained on the basis of differences in initial chill rates between treatments.

Characterization of microwave-sintered ceramic composites

1993 ◽  
Vol 51 ◽  
pp. 1136-1137
Author(s):  
X. Zhang ◽  
Y. Pan ◽  
T.T. Meek
Keyword(s):  
Matrix Material ◽  
Maximum Output Power ◽  
Ceramic Matrix Composite ◽  
Ceramic Composites ◽  
Processing Technique ◽  
Structure Characterization ◽  
Alumina Powder ◽  
Maximum Output ◽  
Sintered Ceramic

Industrial microwave heating technology has emerged as a new ceramic processing technique. The unique advantages of fast sintering, high density, and improved materials properties makes it superior in certain respects to other processing methods. This work presents the structure characterization of a microwave sintered ceramic matrix composite.Commercial α-alumina powder A-16 (Alcoa) is chosen as the matrix material, β-silicon carbide whiskers (Third Millennium Technologies, Inc.) are used as the reinforcing element. The green samples consisted of 90 vol% Al2O3 powder and 10 vol% ultrasonically-dispersed SiC whiskers. The powder mixture is blended together, and then uniaxially pressed into a cylindrical pellet under a pressure of 230 MPa, which yields a 52% green density. The sintering experiments are carried out using an industry microwave system (Gober, Model S6F) which generates microwave radiation at 2.45 GHz with a maximum output power of 6 kW. The composites are sintered at two different temperatures (1550°C and 1650°C) with various isothermal processing time intervals ranging from 10 to 20 min.

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

scholarly journals Biochemical and Taxonomic Characterization of Novel Haloarchaeal Strains and Purification of the Recombinant Halotolerant α-Amylase Discovered in the Isolate

2020 ◽  
Vol 11 ◽  
Author(s):  
Dipesh Kumar Verma ◽  
Gunjan Vasudeva ◽  
Chandni Sidhu ◽  
Anil K. Pinnaka ◽  
Senthil E. Prasad ◽  
...  
Keyword(s):  
Taxonomic Characterization

scholarly journals Development and Validation of a Nested-PCR-Denaturing Gradient Gel Electrophoresis Method for Taxonomic Characterization of Bifidobacterial Communities

2003 ◽  
Vol 69 (11) ◽  
pp. 6380-6385 ◽  
Author(s):  
R. Temmerman ◽  
L. Masco ◽  
T. Vanhoutte ◽  
G. Huys ◽  
J. Swings
Keyword(s):  
Nested Pcr ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Temporal Changes ◽  
Rrna Gene ◽  
Specific Pcr ◽  
Gradient Gel Electrophoresis ◽  
Species Specific ◽  
Taxonomic Characterization

ABSTRACT The taxonomic characterization of a bacterial community is difficult to combine with the monitoring of its temporal changes. None of the currently available identification techniques are able to visualize a “complete” community, whereas techniques designed for analyzing bacterial ecosystems generally display limited or labor-intensive identification potential. This paper describes the optimization and validation of a nested-PCR-denaturing gradient gel electrophoresis (DGGE) approach for the species-specific analysis of bifidobacterial communities from any ecosystem. The method comprises a Bifidobacterium-specific PCR step, followed by purification of the amplicons that serve as template DNA in a second PCR step that amplifies the V3 and V6-V8 regions of the 16S rRNA gene. A mix of both amplicons is analyzed on a DGGE gel, after which the band positions are compared with a previously constructed database of reference strains. The method was validated through the analysis of four artificial mixtures, mimicking the possible bifidobacterial microbiota of the human and chicken intestine, a rumen, and the environment, and of two fecal samples. Except for the species Bifidobacterium coryneforme and B. indicum, all currently known bifidobacteria originating from various ecosystems can be identified in a highly reproducible manner. Because no further cloning and sequencing of the DGGE bands is necessary, this nested-PCR-DGGE technique can be completed within a 24-h span, allowing the species-specific monitoring of temporal changes in the bifidobacterial community.

Characterization of dopamine receptor of type 4 (D4) in human frontal cortex post-mortem

2001 ◽  
Vol 11 ◽  
pp. S282-S283
Author(s):  
D. Marazziti ◽  
I. Masala ◽  
G. Giannaccini ◽  
E. Di Nasso ◽  
L. Betti ◽  
...  
Keyword(s):  
Frontal Cortex ◽  
Dopamine Receptor ◽  
Post Mortem ◽  
Human Frontal Cortex

Taxonomic characterization of members of the genus Corallococcus: Molecular divergence versus phenotypic coherency

2007 ◽  
Vol 30 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Erko Stackebrandt ◽  
Orsola Päuker ◽  
Ulrike Steiner ◽  
Peter Schumann ◽  
Bettina Sträubler ◽  
...  
Keyword(s):  
Molecular Divergence ◽  
Taxonomic Characterization

scholarly journals Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.

2004 ◽  
Vol 54 (4) ◽  
pp. 1055-1061 ◽  
Author(s):  
Carole Feurer ◽  
Dominique Clermont ◽  
François Bimet ◽  
Adina Candréa ◽  
Mary Jackson ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Fatty Acid Content ◽  
Food Samples ◽  
Cell Protein ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Taxonomic Analysis ◽  
Gene Sequence Analysis ◽  
Taxonomic Characterization

Nine unidentified Gram-positive, lipophilic corynebacteria were isolated from clinical and food samples and subjected to a polyphasic taxonomic analysis. The bacteria were distinguished from Corynebacterium species with validly published names by biochemical tests, fatty acid content and whole-cell protein analysis. Comparative 16S rRNA gene sequence analysis demonstrated unambiguously that the nine strains were related phylogenetically to the species ‘Corynebacterium tuberculostearicum’ and represented a distinct subline within the genus Corynebacterium. On the basis of both phenotypic and phylogenetic evidence, the formal description of Corynebacterium tuberculostearicum sp. nov. is proposed. The type strain of C. tuberculostearicum is Medalle XT (=LDC-20T=CIP 107291T=CCUG 45418T=ATCC 35529T).

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