Growth and Death of Selected Microorganisms in Ultrafiltered Milk

1986 ◽  
Vol 49 (3) ◽  
pp. 233-235 ◽  
Author(s):  
PATRICIA HAGGERTY ◽  
NORMAN N. POTTER

Studies were made to compare the growth and death of Staphylococcus aureus, Streptococcus faecalis and Escherichia coli in skim milk concentrated by ultrafiltration to that in unconcentrated skim milk. Skim milk was volume concentrated to 2× in laboratory-scale stirred UF cells. Behavior of the organisms was analyzed in four inoculated milk samples: 2× retentate, 1× water-diluted retentate, milk equivalent (retentate plus permeate) and unconcentrated skim milk. Growth of each organism and of total aerobes did not vary in the four milk samples at either 7 or 13°C. For S. faecalis and E. coli, D-values for samples heated to 62.7°C did not significantly differ in the four milk samples (p>0.01). The D-value of S. aureus in water-diluted retentate was slightly but significantly lower than those in the other three milk samples (p<0.01), possibly due to the lowered lactose level in this sample.

2011 ◽  
Vol 94 (1) ◽  
pp. 118-127
Author(s):  
Marlus Chorilli ◽  
Gislaine Ricci Leonardi ◽  
Hérida Regina Nunes Salgado ◽  
Maria Virgínia Scarpa

Abstract The objective of this work was to evaluate the preservative effectiveness of liquid crystalline systems containing retynil palmitate (RP) by the challenge test (CT) and D-value. A system was developed containing water, silicon glycol copolymer, and polyether functional siloxane with 1% RP added. The analyses were carried out by methods in the U.S. Pharmacopeia (USP 31, 2008) using the microorganisms Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. The CT showed that after 7 days, all microorganisms were eliminated except A. niger, which maintained viability for at least 28 days after inoculation. Moreover, the microorganisms E. coli, P. aeruginosa, S. aureus, C. albicans and A. niger presented different growth behaviors, evidenced by differences among the D-values calculated. It was concluded that the CT and D-value were efficient methods for evaluation of the preservative property of these formulations.


Author(s):  
Susmita Phattepuri ◽  
Prince Subba ◽  
Arjun Ghimire ◽  
Shiv Nandan Sah

Milk is an excellent medium for the growth of many bacteria. This study aimed to determine antibiotic profiling and thermal inactivation of Staphylococcus aureus and Escherichia coli isolated from raw milk of Dharan. Total viable count, total Staphylococcal count, and total coliform count were carried out by conventional microbiological methods. Identification was done on the basis of Gram staining and biochemical tests. The antibiotic susceptibility test of the isolates carried out by the modified Kirby-Baur disc diffusion method. Thermal inactivation of S. aureus and E. coli were carried out by subjecting to thermal treatment in a water bath. Total plate count ranged from 204×104 CFU/mL to 332×105 CFU/mL. Total staphylococcal count and total coliform count ranged from 14×105 CFU/mL to 8×106 CFU/mL and 11×104 CFU/mL to 3×106 CFU/mL respectively. S. aureus showed an increasing resistance patterns towards Ampicillin, Cefotixin, Carbenicillin and Cefotaxime. Ciprofloxacin, Erythromycin, Amikacin, Gentamycin, Azithromycin, and Chloramphenicol were found to be effective against S. aureus. All the E. coli isolates were resistant to Ampicillin and least resistant to Cefotixin. Chloramphenicol, Amikacin, Azithromycin, and Nalidixic acid were found highly effective to E. coli. The D-values for S. aureus at 56°C, 58°C and 60°C were 1.36 min, 1.19 min, and 1.09 min respectively. The Z-value was 14.92°C. While D-values were obtained as 0.98 min, 0.75 min, and 0.57 min for E. coli at 56° C, 58° C and 60° C respectively, and Z-value was 9.75° C. Hence, S. aureus was found to be more heat resistant than E. coli.


1980 ◽  
Vol 28 (3) ◽  
pp. 762-765
Author(s):  
A Cuffini ◽  
N A Carlone ◽  
G Forni

The phagocytic capacity of macrophages from C3H/H3J mice was assessed against lipopolysaccharide-producing (Escherichia coli) and -nonproducing (Staphylococcus aureus) bacteria. Despite their gene-coded unresponsiveness to lipopolysaccharide endotoxin and lymphokines and their defective tumoricidal activity, proteose peptone-induced C3H/HeJ macrophages did not display a defective phagocytic capacity, but rather displayed an enhanced phagocytosis of both bacterial strains compared with macrophages from closely related C3H/HeN mice. Unstimulated peritoneal resident C3H/HeJ macrophages, on the other hand, displayed a normal phagocytic activity toward E. coli and enhanced phagocytosis toward S. aureus.


1998 ◽  
Vol 61 (9) ◽  
pp. 1184-1186 ◽  
Author(s):  
NICOLE C. WILLIAMS ◽  
STEVEN C. INGHAM

On a beef carcass, Escherichia coli may sequentially encounter acid- and heat-intervention steps. This study tested whether acid stress (1.5% [vol/vol] acetic acid, pH 4.0, 37°C, 15 min) would enhance subsequent heat resistance of E. coli. Initially, cells (E. coli O157:H7 ATCC 43894, nonpathogenic E. coli B [strain FRIK-124], and rpoS-deficient mutant 813-6 [derived from E. coli O157:H7 ATCC 43895]) were acid stressed and transferred to 54°C tiypticase soy broth (TSB), and survivors were immediately enumerated after at least three intervals of 12, 2, and 6 min, respectively, by plating. The ATCC 43894 and 813-6 strains survived the acid stress but strain FRIK-124 did not. Acid-stressed ATCC 43894 had significantly lower D values than the non-acid-stressed controls. Strain 813-6 had significantly lower D values than strain ATCC 43894, with no significant difference between acid-stressed and non-acid-stressed cells. In a second experiment, cooling of cells prior to plating resulted in an increased D value for acid-stressed ATCC 43894 cells, such that it was not significantly different from the D value for non-acid-stressed Controls. Using this protocol, there was no significant difference in D values between acid-stressed and non-acid-stressed ATCC 43894 cells in prewarmed TSB (54, 58, and 62°C), in prewarmed ground beef slurry (GBS; 58°C), or in TSB and GBS inoculated at 5°C and heated to 58°C. The acid stress tested does not enhance subsequent heat resistance of E. coli.


1977 ◽  
Vol 23 (6) ◽  
pp. 716-720
Author(s):  
A. Chopin ◽  
G. Mocquot ◽  
Y. Le Graet

In this paper a method which allows the measure of microbial death rate during spray-drying by means of a streptomycin-resistant mutant that can be grown on a streptomycin-containing agar is described. Plate counts of Microbacterium lacticum, Escherichia coli, and Staphylococcus aureus recovered from skim milk powders were done on plate count agar in the presence and absence of streptomycin and on various selective media. The powders were produced from evaporated milk previously inoculated with those organisms.Our results showed that the proposed method allows the recovery of 78% of M. lacticum, 61% of E. coli, and 100% of S. aureus that survived spray-drying. Recoveries of surviving E. coli on violet bile agar and brilliant green bile 2% were 34% and 29% respectively. Baird-Parker and mannitol salt agar media allow the recovery of all surviving S. aureus, thus showing that S. aureus cells did not lose their ability to grow in media containing 7.5% NaCl. Our results show that physiological injury of the cells during spray-drying differs from injury due to heating only. [Traduit par le journal]


2018 ◽  
Vol 12 (07) ◽  
pp. 533-541 ◽  
Author(s):  
Walid Elmonir ◽  
Etab Abo-Remela ◽  
Azza Sobeih

Introduction: Milk is an important food in Egypt and most of it is sold as raw milk in informal markets. Methodology: This study was conducted to investigate the public health risks of Escherichia coli and Staphylococcus aureus in milk sold in informal markets in Egypt. A total of 121 milk samples were analyzed for occurrence, virulence genes and antibiotic resistance of E. coli and S. aureus. Results: A total of 35/121 (28.9%) of milk samples were contaminated with 16/121 (13.2%) E. coli, 22/121 (18.2%) S. aureus, and 3/121 (2.5%) both isolates. Shiga-toxin producing E. coli (STEC), Enterotoxigenic E. coli (ETEC) and Enterotoxigenic S. aureus were detected in 5/121 (4.1%), 2/121 (1.7%) and 8/121 (6.6%) of the examined milk samples, respectively. Multiple drug resistances (MDRs) were showed by 14/16 (87.5%) and 21/22 (95.5%) of E. coli and S. aureus isolates, respectively. E. coli isolates showed high resistance for cephalothin (87.5%), ampicillin (68.8%) and tetracycline (68.8%), but were sensitive for gentamicin and chloramphenicol. Resistance phenotypes of E. coli were diverse; however, STEC isolates were significantly associated with co-resistance to cephalothin, ampicillin and tetracycline (P< 0.05). Two (9.1%) of S. aureus isolates were methicillin-resistant (MRSA) but sensitive to gentamicin (GS-MRSA). Five (22.7%) of S. aureus isolates were gentamicin-resistant methicillin-sensitive S. aureus (GR-MSSA). S. aureus isolates also showed high resistance for ampicillin (100%), tetracycline (90.1%) and sulfamethoxazole-trimethoprim (90.1%). Conclusion: These findings highlighted the potential public health hazards of E. coli and S. aureus pathogens in raw milk sold in informal markets in Egypt.


Author(s):  
S. DHANARAJ ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI

Objective: To compare the antibacterial activity of honey against aerobic and anaerobic bacteria. Methods: Honey is extracted from the honey comb by trained persons. Antimicrobial activity of honey is performed by Agar Cup Diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and Clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to honey whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to honey. Conclusion: Due to its vast antibacterial activity of honey, it can be used along with other antibiotics to increase its efficiency.


Author(s):  
K. G. DHANUSH ◽  
S. S. M. UMAMAGESWARI ◽  
M. MALAVIKA ◽  
G. BHUVANESHWARI

Objective: To compare the antibacterial activity of garlic against aerobic and anaerobic bacteria. Methods: Antimicrobial activity of garlic is performed by Agar cup diffusion technique for 3 bacteria Staphylococcus aureus, E. coli and clostridium perfringens. Results: By performing the technique with proper guidance, it is observed that the Staphylococcus aureus specimen shows sensitivity to garlic whereas the other two specimens Escherichia coli and clostridium perfringens doesn’t show any sensitivity to garlic. Conclusion: Due to its vast antibacterial activity of garlic, it can be used along with other antibiotics to increase its efficiency.


2022 ◽  
Author(s):  
Zahran Khaldi ◽  
Mounir Nafti ◽  
Mohamed Tabarek Jilani

Abstract Characteristics and quality aspects of milk from native ovine queue fine de l’Ouest (QFO) and the local goat population were investigated and compared with those of the local Maghrebi camel. A total of 378 individual milk samples were collected from lactating animals reared in the continental oasis region of Tunisia. Samples were analyzed for physical parameters (pH, density, and acidity), chemical composition (dry matter, fat, protein, lactose, casein, ash, and casein-protein ratio), mineral concentrations (Ca, P, Na, and K) and microbiological features (total mesophilic aerobic bacteria (TMAB), total coliform count (TCC), lactic acid bacteria (LAB), sulphite-reducing Clostridium (CSR), yeast and molds (Y/M), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Salmonella) according to standard methods. The results obtained for milk characteristics revealed noticeable disparities between the three species. The mean values of pH, density, and acidity in milk collected from sheep appeared higher than those in milk collected from goat species. Compared to the camel populations, sheep species produced milk with similar pH but higher density and acidity. Between camel and goat species, pH and acidity were higher in Negga, while the density was similar. For milk composition, the results showed a remarkable variation among all studied species and an obvious superiority of the ovine species over the caprine and camel populations in all the chemical contents being studied, except for the casein-protein ratio, which is in favor of goat species. The milk of QFO sheep, the richest in casein and protein, was expressed with significantly higher levels of calcium and phosphorus than goat and camel milk. Compared to small ruminants, milk from camels is the richest in Na and K. Additionally, more Ca is present in milk from camels than goats. Goat milk, the poorest type of milk in Ca and Na, contains on average more P than camel milk and more K than sheep's milk. The poor bacteriological quality was that of camel milk for all microbial counts. The microbial quality of goat milk was higher than that of ewe milk based on TMAB, TCC, and E. coli counts, while ovine milk was of better quality, referring to LAB, Y/M, and S. aureus values. No significant differences were found for Staphylococcus aureus and Escherichia coli between the examined species. The obtained results highlighted the complete absence of the two dangerous pathogens Salmonella and CSR in all investigated milk samples. The microbiological examination evidenced that the milk of small ruminant species complies with standard criteria required by Tunisian legislation on the hygiene of milk and dairy products. Regarding camel milk, the microbial analysis revealed poor quality that exceeds standard criteria.


1961 ◽  
Vol 24 (6) ◽  
pp. 172-175 ◽  
Author(s):  
T. R. Oberhofer ◽  
W. C. Frazier

Sixty-six cultures of food microorganisms were screened by spot-plate tests on their ability to influence the growth of four strains of Staphylococcus aureus, two enterotoxigenic and two not. The six test media were selected for differences in complexity and to simulate natural foods. The most consistently inhibitory cultures for S. aureus were: Streptococcus faecium, Streptococcus faecalis, S. faecalis var. liquefaciens, a nisin-producing Streptococcus lactis, and various meat lactobacilli. Other cultures were less consistently inhibitory, and many were not inhibitory or were even stimulatory. Growth of two enterotoxigenic strains of S. aureus in meat infusion broth at 15°, 30° and 44° C. was only moderately reduced by simultaneous growth of Escherichia coli strain Gratia, but was markedly reduced by growth of E. coli H52, especially at 15° C. and 44° C.


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