Association of [32P] with Clostridium botulinum 52A Vegetative Cells Following Growth in a Medium Containing Sodium Dihydrogen [32P]-Pyrophosphate

1986 ◽  
Vol 49 (5) ◽  
pp. 352-354 ◽  
Author(s):  
M. K. WAGNER ◽  
F. F. BUSTA
Keyword(s):  
Stationary Phase ◽  
Clostridium Botulinum ◽  
Cellular Growth ◽  
Vegetative Cells ◽  
Sodium Dihydrogen ◽  
Logarithmic Growth

The association of [32P] with Clostridium botulinum 52A vegetative cells following growth in a medium containing either sodium dihydrogen [32P]-pyrophosphate ([32P]-SAPP) or sodium dihydrogen [32P]-orthophosphate ([32P]-orthophosphate) was studied. Absorbency measurements at 630 nm were used in addition to [32P] recovery in determining [32P] association with cellular growth and metabolism. Radiolabeling experiments showed [32P]-orthophosphate was associated with vegetative cells during logarithmic growth, yet was released once stationary phase was attained or upon lysis. [3P]-SAPP was also associated with cells during growth, but was not released once stationary phase was attained. Results suggested [32P]-SAPP continued to bind cells or other metabolic materials following attainment of the stationary phase of cells. Fractionation of 24 and 48 h-old cultures grown in the presence of [32P]-SAPP showed a higher percentage of [32P] associated with the RNA fraction (3.91 and 2.48%, respectively) compared to the DNA fraction (0.09 and 0.07%, respectively).

Amylopectin accumulation in Clostridium botulinum type E

1968 ◽  
Vol 14 (10) ◽  
pp. 1059-1062 ◽  
Author(s):  
G. A. Strasdine
Keyword(s):  
Maximum Concentration ◽  
Clostridium Botulinum ◽  
Vegetative Cells ◽  
Botulinum Type ◽  
Clostridium Botulinum Type E ◽  
Intracellular Polysaccharide ◽  
Logarithmic Growth

An intracellular polysaccharide composed of glucose subunits and structurally resembling amylopectin was isolated from vegetative cells of Clostridium botulinum type E. Chemical and microscopic methods were employed to demonstrate amylopectin accumulation. Polysaccharide granules appeared in the cytoplasm 6 hours after inoculation, accumulated rapidly during early logarithmic growth, and reached a maximum concentration just before the onset of sporulation. With the exception of two non-toxic strains of C. botulinum type E, all strains examined demonstrated the ability to store a similar polysaccharide. The possibility that amylopectin may function as an endogenous source of carbon and energy is discussed.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Die Bildung von Thymidintriphosphat bei Tetrahymena pyriformis (EHRENBERG) in statistischen und hitzesynchronisierten Kulturen / Formation of Thymidine Triphosphate in statistical and heat synchronized cultures of Tetrahymena pyriformis (EHRENBERG)

1970 ◽  
Vol 25 (5) ◽  
pp. 517-521 ◽  
Author(s):  
Manfred K. Grieshaber ◽  
Franz Duspiva
Keyword(s):  
Heat Treatment ◽  
Enzyme Activity ◽  
Stationary Phase ◽  
Dna Synthesis ◽  
Tetrahymena Pyriformis ◽  
Thymidylate Kinase ◽  
Proteose Peptone ◽  
Thymidine Triphosphate ◽  
Logarithmic Growth ◽  
Synchronized Cultures

The activity of thymidylate kinase is correlated with DNA-synthesis in Tetrahymena. During logarithmic growth it is twice as high as in the stationary phase; in cultures synchronized by heat treatment, the activity of the enzyme increases with the onset of DNA-synthesis. After application of 5 x 10-6 ᴍ Methotrexate, the activity of thymidylate kinase remains unchanged. There is, however, a dramatic increase in enzyme activity when the inhibition of transmethylations is circumvened by adding thymidine and proteose-peptone.

Some Observations on a Perigo-Type Inhibition of Clostridium botulinum in a Simplified Medium

1975 ◽  
Vol 38 (12) ◽  
pp. 762-763 ◽  
Author(s):  
C. N. HUHTANEN
Keyword(s):  
Yeast Extract ◽  
Clostridium Botulinum ◽  
Type A ◽  
Reducing Agents ◽  
Reducing Agent ◽  
Sensitive Assay ◽  
Vegetative Cells ◽  
Botulinum Type ◽  
Better Than

A rapid and sensitive assay for Perigo factor was developed using a medium of 0.5% yeast extract and tryptone, 0.2% glucose, 0.12% K2HPO4 and 0.1% cysteine HCI or sodium thioglycollate and vegetative cells of Clostridium botulinum type A. Yeast extract or tryptone, together with a reducing agent (cysteine, sodium thioglycollate, or glucose autoclaved with the medium), produced a Perigo inhibitor when autoclaved at 15 psi for 15 min with NaNO2. Tryptone was more active than yeast extract as a source of the Perigo inhibitor; of the reducing agents tested cysteine was more effective in producing Perigo-type inhibition than thioglycollate and either was better than glucose autoclaved with the medium.

Acid Phosphatase Activity in Vegetative Cells and Spores of Clostridium botulinum Type E

1971 ◽  
Vol 28 (11) ◽  
pp. 1817-1820 ◽  
Author(s):  
G. A. Strasdine ◽  
Joanne M. Melville
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Clostridium Botulinum ◽  
Specific Activity ◽  
Growth Media ◽  
Ph Optimum ◽  
Vegetative Cells ◽  
Botulinum Type ◽  
Clostridium Botulinum Type E

Acid phosphatase activity with a pH optimum of 5 was demonstrated in vegetative cells, spores, and germinated spores of Clostridium botulinum type E (Minnesota). The enzyme was present in the cells during all stages of growth and was insensitive to the orthophosphate concentration of the growth media. Specific activity of the enzyme increased during growth coincident with a loss in inorganic phosphate from the acid-soluble cell fraction. Magnesium or manganese was required for maximum enzyme activity. Acid phosphatase in crude spore extracts was more heat-stable than in extracts obtained from vegetative cells.

scholarly journals Changes in Cell Size and DNA Content inSulfolobus Cultures during Dilution and Temperature Shift Experiments

1999 ◽  
Vol 181 (18) ◽  
pp. 5669-5675 ◽  
Author(s):  
Karin Hjort ◽  
Rolf Bernander
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Stationary Phase ◽  
Growth Medium ◽  
Temperature Shift ◽  
Chromosome Replication ◽  
Cellular Growth ◽  
Inhibition Of Growth ◽  
Ice Water ◽  
Fresh Growth Medium

ABSTRACT Stationary-phase cultures of different hyperthermophilic species of the archaeal genus Sulfolobus were diluted into fresh growth medium and analyzed by flow cytometry and phase-fluorescence microscopy. After dilution, cellular growth started rapidly but no nucleoid partition, cell division, or chromosome replication took place until the cells had been increasing in size for several hours. Initiation of chromosome replication required that the cells first go through partition and cell division, revealing a strong interdependence between these key cell cycle events. The time points at which nucleoid partition, division, and replication occurred after the dilution were used to estimate the relative lengths of the cell cycle periods. When exponentially growing cultures were diluted into fresh growth medium, there was an unexpected transient inhibition of growth and cell division, showing that the cultures did not maintain balanced growth. Furthermore, when cultures growing at 79°C were shifted to room temperature or to ice-water baths, the cells were found to “freeze” in mid-growth. After a shift back to 79°C, growth, replication, and division rapidly resumed and the mode and kinetics of the resumption differed depending upon the nature and length of the shifts. Dilution of stationary-phase cultures provides a simple protocol for the generation of partially synchronized populations that may be used to study cell cycle-specific events.

scholarly journals Nisin Resistance in Clostridium botulinum Spores and Vegetative Cells.

1997 ◽  
Vol 63 (7) ◽  
pp. 2654-2659 ◽  
Author(s):  
A S Mazzotta ◽  
A D Crandall ◽  
T J Montville
Keyword(s):  
Clostridium Botulinum ◽  
Vegetative Cells
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