Comparison between VIDAS Automatic Enzyme-Linked Fluorescent Immunoassay and Culture Method for Salmonella Recovery from Pork Carcass Sponge Samples

2002 ◽  
Vol 65 (10) ◽  
pp. 1656-1659 ◽  
Author(s):  
KUANG-SHENG YEH ◽  
CHIN-EN TSAI ◽  
SHIH-PING CHEN ◽  
CHAO-WEI LIAO
Keyword(s):  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Automated System ◽  
Rapid Screening ◽  
Assay Method ◽  
Intensive Culture ◽  
Negative Results ◽  
False Negative Results ◽  
Potential Alternative

VIDAS Salmonella (VIDAS-SLM) is an automated system that uses the enzyme-linked fluorescent assay method to detect Salmonella species. This study evaluated the efficacy of the VIDAS-SLM method in detecting Salmonella species in pork carcass sponge samples gathered from 10 slaughter plants in Taiwan. Two hundred fifty-seven pork carcass sponge samples were screened by the VIDAS-SLM method and by the culture method in parallel. While 18 sponge samples were found to test positive by both methods, the VIDAS-SLM method detected four additional positive samples for which the culture method failed to recover Salmonella. The specificity of the VIDAS-SLM method was found to be 0.98, and its sensitivity was 1.0, since no false-negative results occurred. Artificially inoculated Salmonella at concentrations as low as 5.0 × 100 CFU/ml was detected in the heat-inactivated sponge sample in the presence or absence of 5.0 × 104 CFU of Citrobacter freundii per ml. Thus, the VIDAS-SLM method is a rapid screening method and a potential alternative to the time- and labor-intensive culture method.

Improved Hydrophobic Grid Membrane Filter Method, Using EF-18 Agar, for Detection of Salmonella in Foods: Collaborative Study

1990 ◽  
Vol 73 (5) ◽  
pp. 734-742
Author(s):  
Phyllis Entis
Keyword(s):  
Membrane Filter ◽  
Collaborative Study ◽  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Soy Flour ◽  
Poultry Meat ◽  
Filter Method ◽  
Negative Results ◽  
False Negative Results

Abstract A collaborative study was carried out in 30 laboratories to validate Improvements to the official final action hydrophobic grid membrane filter (HGMF) screening method for Salmonella in foods, 985.42, by comparing the performance of the improved HGMF method against that of the AOAC/BAM conventional culture method. Six products were Included In the collaborative study: milk chocolate, raw deboned poultry meat, black pepper, soy flour, egg yolk powder, and nonfat dry milk. The raw deboned poultry meat was naturally contaminated with Salmonella, and the remaining 5 products were each Inoculated In advance with low levels of Individual Salmonella serotypes. The AOAC/BAM method produced 11 false negative results and the Improved HGMF method produced 18 false negative results. The improved HGMF Salmonella method has been approved Interim official first action for all foods to replace the HGMF official final action method, 985.42.

scholarly journals Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria

1976 ◽  
Vol 3 (1) ◽  
pp. 42-46
Author(s):  
D N Alexander ◽  
G M Ederer ◽  
J M Matsen
Keyword(s):  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Clinical Microbiology Laboratory ◽  
University Of Minnesota ◽  
Atp Assay ◽  
Negative Results ◽  
False Negative Results ◽  
Significant Bacteriuria ◽  
Urine Specimens

The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine. This rapid method of screening urine specimens for bacteriuria was evaluated by using 348 urine specimens submitted to the clinical microbiology laboratory at the University of Minnesota Hospitals for routine culture using the calibrated loop-streak plate method. There was 89.4% agreement between the culture method and the ATP assay, with 7.0% false positive and 27.0% false negative results from the ATP assay using 10(5) organisms/ml of urine or greater as positive for significant bacteriuria and less than 10(5) organisms/ml as negative for significant bacteriuria.

Non-chlamydial non-gonococcal urethritis or undiagnosed chlamydial urethritis?

2006 ◽  
Vol 17 (5) ◽  
pp. 296-298 ◽  
Author(s):  
K Manavi ◽  
A McMillan ◽  
H Young
Keyword(s):  
Chlamydial Infection ◽  
False Negative ◽  
Culture Method ◽  
Sexual Partners ◽  
Chain Reaction ◽  
Sexually Transmitted ◽  
Female Partners ◽  
Gonococcal Urethritis ◽  
Negative Results ◽  
False Negative Results

The aim of this study is to investigate the prevalence of sexually transmitted infections (STI) in the partners of men with non-chlamydial, non-gonococcal urethritis (NCNGU). Observational study of the sexual partners of men with NCNGU diagnosed in the Department of Genitourinary Medicine, Edinburgh between 1 June 2002 and 31 December 2003. The diagnosis of chlamydial infection was based on ligase chain reaction (LCx) between June 2002 and March 2003, and on polymerase chain reaction (PCR) thereafter. Gonococcal infection was diagnosed with culture method. Sexual partners of 99 (25%) of the 403 heterosexual men diagnosed with NCNGU were screened. Chlamydial infection was detected in 19 (19%) of the female sexual partners. Higher proportion of female partners of symptomatic men (15/51) had chlamydial infection compared with that of partners of asymptomatic men (4/48) ( P < 0.005). NCNGU may be related to false-negative results of chlamydial diagnostic tests. Screening and treatment of sexual partners of men with NCNGU is therefore necessary.

Comparison of Colorimetric Monoclonal Enzyme Immunoassay Screening Methods for Detection of Salmonella in Foods

1990 ◽  
Vol 73 (1) ◽  
pp. 43-50
Author(s):  
Michael S Curiale ◽  
Mary Joan Klatt ◽  
Barbara J Robison ◽  
Lisa T Beck
Keyword(s):  
Enzyme Immunoassay ◽  
Magnetic Beads ◽  
False Negative ◽  
Screening Method ◽  
False Negative Rate ◽  
Culture Method ◽  
Food Samples ◽  
Assay Method ◽  
Screening Methods ◽  
Well Assay

Abstract A colorimetric enzyme immunoassay (EIA) method for detection of Salmonella in foods has been compared to the AOAC colorimetric monoclonal EIA screening method (986.35,15th ed.; 46.B21-46.B29, 14th ed.). The assays use the same monoclonal antibodies and have similar reactivity toward Salmonella. However, the new assay uses antibody-coated microtiter wells instead of coated magnetic beads to capture Salmonella antigens. Compared with the bead assay, the coated-well assay format requires significantly less time to complete, and was consistently able to detect lower levels of Salmonella In mixed culture. Compared to the standard AOAC culture method for food samples, the plate assay was as productive. No false negatives were obtained by the immunoassay; the false negative rate was 1.1% by the culture method. The rate of agreement between the 2 methods was 99.1 %. The official final action bead assay method for Salmonella in foods, 986.35, and the same assay for use with low-moisture foods, 987.11, have been modified official first action to use antibody-coated microtiter strip-wells

scholarly journals Simplified microscopy for rapid detection of significant bacteriuria in random urine specimens

1978 ◽  
Vol 7 (3) ◽  
pp. 286-289
Author(s):  
G K Barbin ◽  
J D Thorley ◽  
J A Reinarz
Keyword(s):  
False Negative ◽  
Rapid Screening ◽  
Negative Results ◽  
Microscopy Technique ◽  
Gram Staining ◽  
False Negative Results ◽  
Significant Bacteriuria ◽  
Urine Microscopy ◽  
Positive Results ◽  
Urine Specimens

Simplified urine microscopy, nitrite testing, and dipstick culture were compared with urine loop streak culture colony counts in 219 random voided specimens to determine the accuracy of the three rapid screening techniques. Nitrite testing resulted in 65% false negative results, which could not be significantly improved by incubation at 37 degrees C but which could be improved by adding nitrate substrate before incubation. Dipstick culture could not be quantitated until after 18 h of incubation. A new, simplified microscopy technique, using unspun, unstained urine, resulted in 4% false negative results and 4% false positive results in specimens containing over 10(5) organisms per ml and was the best method Centrifuges, Gram staining reagents, and counting chambers are not necessary for accurate microscopic screening of random urine specimens for the presence of bacteriuria by this technique, and the results are immediately available.

Effect of non-fat dry milk on recovery of staphylococcal thermonuclease from foods

1979 ◽  
Vol 25 (1) ◽  
pp. 44-46 ◽  
Author(s):  
C. E. Park ◽  
H. B. El Derea ◽  
M. K. Rayman
Keyword(s):  
False Negative ◽  
Ground Beef ◽  
Acid Precipitation ◽  
Food Samples ◽  
Assay Method ◽  
Negative Results ◽  
False Negative Results ◽  
Dry Milk ◽  
Egg Products ◽  
Subsequent Acid

Modification of the method of Tatini et al. (1976) by addition of non-fat dry milk (NFDM) to food samples and subsequent acid precipitation at pH 3.8 enhanced the recovery of staphylococcal thermonuclease (TNase) from most of 37 foods tested. The modified TNase assay method allowed detection of 10 ng (0.002 units) of the enzyme per gram of each of the following foods: ground beef, boiled egg products, whey powder, fruit-containing yogurt, dressings and spreads, potato and egg salads, and pastas, all of which gave false-negative results without NFDM.

scholarly journals The promise, perceptions, and pitfalls of immunoassays for autoantibody testing in myositis

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Sarah L. Tansley ◽  
◽  
Julia Snowball ◽  
John D. Pauling ◽  
Anya Lissina ◽  
...  
Keyword(s):  
Decision Making ◽  
Clinical Decision Making ◽  
False Negative ◽  
Clinical Decision ◽  
Assay Method ◽  
Negative Results ◽  
Recommended Treatment ◽  
False Negative Results ◽  
Specific Autoantibody ◽  
Personalized Approach

Abstract Background A myositis-specific autoantibody can now be identified in the majority of patients with myositis. They identify homogeneous patient subgroups and are key tools in developing a personalized approach to disease management. There is substantial clinical interest in exploiting myositis autoantibodies as biomarkers, and consequently, a large number of commercial assays have been developed for their detection. These assays are already in widespread clinical use. In order to better understand perceived concerns from the international myositis community in relation to the reliability of these assays and how they are being used, we conducted a survey of international myositis experts, all of whom were members of the International Myositis Assessment and Clinical Studies group. Results We collected data on the types of assay used, manufacturers, and the nature of the report provided by different laboratories and received 111 complete responses. Respondents also provided information on how they used the different assays, their confidence in the results, and how this influenced their clinical practice. Enzyme immunoassay/ELISA was the most popular assay method used worldwide followed by line blot. Line blot was the most popular method used in Europe. Despite concerns from over 80% of respondents regarding false-positive and false-negative results with the assay used by their laboratory, over 80% reported that the identification of a myositis autoantibody influenced their diagnostic confidence, the information they provided to a patient, and their recommended treatment. Conclusions In spite of ongoing concerns from the majority of users regarding the reliability of the results, myositis-specific autoantibody testing, using commercial immunoassays, is being used globally to inform clinical decision-making. These findings highlight the need for urgent guidance on the use of myositis autoantibody testing and on the interpretation of results. Knowledge of the reliability of currently available assays is essential given the importance already placed on myositis-specific autoantibodies as clinical decision-making tools.

scholarly journals Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn

2010 ◽  
Vol 53 (3) ◽  
pp. 687-692 ◽  
Author(s):  
Elisabete Yurie Sataque Ono ◽  
Marcelo da Silva ◽  
Ricardo Marcelo Reche Ribeiro ◽  
Mario Augusto Ono ◽  
Luciana Hayashi ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
False Negative ◽  
Screening Method ◽  
Yellow Fluorescence ◽  
Negative Results ◽  
Greenish Yellow ◽  
False Negative Results ◽  
Fluorescence Test ◽  
Layer Chromatography

In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry, with levels ranging from 1.0 to 58.66 µg/kg (mean 17.14 ± 17.81 µg/kg). The results indicated a good correlation (ρ = 0.97) between TLC and spectrofluorimetry for aflatoxin determination in naturally contaminated corn. The bright greenish-yellow fluorescence test was simple and quick, but it showed 20% false-negative results, suggesting its use only as screening method for detecting the suspected lots of grains that should be tested further for aflatoxin by more sensitive methods.

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