HIGH RATE OF FALSE-NEGATIVE RESULTS OF THE RECTAL SWAB CULTURE METHOD IN DETECTION OF GASTROINTESTINAL COLONIZATION WITH VANCOMYCIN-RESISTANT ENTEROCOCCI

2002 ◽  
Vol 21 (6) ◽  
pp. 578-579
Author(s):  
&NA;
Keyword(s):  
Rectal Swab ◽  
False Negative ◽  
Culture Method ◽  
High Rate ◽  
Negative Results ◽  
Vancomycin Resistant Enterococci ◽  
False Negative Results ◽  
Vancomycin Resistant ◽  
Gastrointestinal Colonization

Non-chlamydial non-gonococcal urethritis or undiagnosed chlamydial urethritis?

2006 ◽  
Vol 17 (5) ◽  
pp. 296-298 ◽  
Author(s):  
K Manavi ◽  
A McMillan ◽  
H Young
Keyword(s):  
Chlamydial Infection ◽  
False Negative ◽  
Culture Method ◽  
Sexual Partners ◽  
Chain Reaction ◽  
Sexually Transmitted ◽  
Female Partners ◽  
Gonococcal Urethritis ◽  
Negative Results ◽  
False Negative Results

The aim of this study is to investigate the prevalence of sexually transmitted infections (STI) in the partners of men with non-chlamydial, non-gonococcal urethritis (NCNGU). Observational study of the sexual partners of men with NCNGU diagnosed in the Department of Genitourinary Medicine, Edinburgh between 1 June 2002 and 31 December 2003. The diagnosis of chlamydial infection was based on ligase chain reaction (LCx) between June 2002 and March 2003, and on polymerase chain reaction (PCR) thereafter. Gonococcal infection was diagnosed with culture method. Sexual partners of 99 (25%) of the 403 heterosexual men diagnosed with NCNGU were screened. Chlamydial infection was detected in 19 (19%) of the female sexual partners. Higher proportion of female partners of symptomatic men (15/51) had chlamydial infection compared with that of partners of asymptomatic men (4/48) ( P < 0.005). NCNGU may be related to false-negative results of chlamydial diagnostic tests. Screening and treatment of sexual partners of men with NCNGU is therefore necessary.

Improved Hydrophobic Grid Membrane Filter Method, Using EF-18 Agar, for Detection of Salmonella in Foods: Collaborative Study

1990 ◽  
Vol 73 (5) ◽  
pp. 734-742
Author(s):  
Phyllis Entis
Keyword(s):  
Membrane Filter ◽  
Collaborative Study ◽  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Soy Flour ◽  
Poultry Meat ◽  
Filter Method ◽  
Negative Results ◽  
False Negative Results

Abstract A collaborative study was carried out in 30 laboratories to validate Improvements to the official final action hydrophobic grid membrane filter (HGMF) screening method for Salmonella in foods, 985.42, by comparing the performance of the improved HGMF method against that of the AOAC/BAM conventional culture method. Six products were Included In the collaborative study: milk chocolate, raw deboned poultry meat, black pepper, soy flour, egg yolk powder, and nonfat dry milk. The raw deboned poultry meat was naturally contaminated with Salmonella, and the remaining 5 products were each Inoculated In advance with low levels of Individual Salmonella serotypes. The AOAC/BAM method produced 11 false negative results and the Improved HGMF method produced 18 false negative results. The improved HGMF Salmonella method has been approved Interim official first action for all foods to replace the HGMF official final action method, 985.42.

Failure of Oral Antimicrobial Agents in Eradicating Gastrointestinal Colonization With Vancomycin-Resistant Enterococci

2002 ◽  
Vol 23 (1) ◽  
pp. 43-44 ◽  
Author(s):  
Ray Hachem ◽  
Issam Raad
Keyword(s):  
Antimicrobial Agents ◽  
Group Versus ◽  
Control Group ◽  
Similar Frequency ◽  
Negative Results ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Gastrointestinal Colonization ◽  
Stool Cultures

AbstractEradication of gastrointestinal colonization with vancomycin-resistant enterococci (VRE) using a combination of oral bacitracin and gentamicin was evaluated. Twenty-eight evaluable treated patients were matched with 28 control patients. After 3 months of follow-up, 5 patients (18%) in the treatment group versus 1 patient (4%) in the control group (P= .2) had negative results on stool cultures, with a similar frequency of VRE bacteremia (P= .8). The use of oral bacitracin plus gentamicin did not reduce VRE colonization or bacteremia.

Comparison between VIDAS Automatic Enzyme-Linked Fluorescent Immunoassay and Culture Method for Salmonella Recovery from Pork Carcass Sponge Samples

2002 ◽  
Vol 65 (10) ◽  
pp. 1656-1659 ◽  
Author(s):  
KUANG-SHENG YEH ◽  
CHIN-EN TSAI ◽  
SHIH-PING CHEN ◽  
CHAO-WEI LIAO
Keyword(s):  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Automated System ◽  
Rapid Screening ◽  
Assay Method ◽  
Intensive Culture ◽  
Negative Results ◽  
False Negative Results ◽  
Potential Alternative

VIDAS Salmonella (VIDAS-SLM) is an automated system that uses the enzyme-linked fluorescent assay method to detect Salmonella species. This study evaluated the efficacy of the VIDAS-SLM method in detecting Salmonella species in pork carcass sponge samples gathered from 10 slaughter plants in Taiwan. Two hundred fifty-seven pork carcass sponge samples were screened by the VIDAS-SLM method and by the culture method in parallel. While 18 sponge samples were found to test positive by both methods, the VIDAS-SLM method detected four additional positive samples for which the culture method failed to recover Salmonella. The specificity of the VIDAS-SLM method was found to be 0.98, and its sensitivity was 1.0, since no false-negative results occurred. Artificially inoculated Salmonella at concentrations as low as 5.0 × 100 CFU/ml was detected in the heat-inactivated sponge sample in the presence or absence of 5.0 × 104 CFU of Citrobacter freundii per ml. Thus, the VIDAS-SLM method is a rapid screening method and a potential alternative to the time- and labor-intensive culture method.

scholarly journals Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria

1976 ◽  
Vol 3 (1) ◽  
pp. 42-46
Author(s):  
D N Alexander ◽  
G M Ederer ◽  
J M Matsen
Keyword(s):  
False Negative ◽  
Screening Method ◽  
Culture Method ◽  
Clinical Microbiology Laboratory ◽  
University Of Minnesota ◽  
Atp Assay ◽  
Negative Results ◽  
False Negative Results ◽  
Significant Bacteriuria ◽  
Urine Specimens

The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine. This rapid method of screening urine specimens for bacteriuria was evaluated by using 348 urine specimens submitted to the clinical microbiology laboratory at the University of Minnesota Hospitals for routine culture using the calibrated loop-streak plate method. There was 89.4% agreement between the culture method and the ATP assay, with 7.0% false positive and 27.0% false negative results from the ATP assay using 10(5) organisms/ml of urine or greater as positive for significant bacteriuria and less than 10(5) organisms/ml as negative for significant bacteriuria.

Contamination of Gowns, Gloves, and Stethoscopes With Vancomycin-Resistant Enterococci

2001 ◽  
Vol 22 (9) ◽  
pp. 560-564 ◽  
Author(s):  
Kimon C. Zachary ◽  
Pamela S. Bayne ◽  
Victoria J. Morrison ◽  
Dale S. Ford ◽  
Leah Christine Silver ◽  
...  
Keyword(s):  
Rectal Swab ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
High Rate ◽  
Care Hospital ◽  
Routine Examination ◽  
Clinical Specimens ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Similar Risk

AbstractObjective:To measure directly the rate of contamination, during routine patient examination, of gowns, gloves, and stethoscopes with vancomycin-resistant enterococci (VRE).Setting:A large, academic, tertiary-care hospital.Patients:Between January 1997 and December 1998, 49 patients colonized or infected with VRE were entered in the study.Design:After routine examination, the examiner's glove fingertips, gown (the umbilical region and the cuffs), and stethoscope diaphragm were pressed onto Columbia colistin-nalidixic acid (CNA) agar plates with 5% sheep blood plus vancomycin 6 ug/mL. The stethoscope diaphragm was sampled again after cleaning with a 70% isopropanol wipe.Results:VRE were isolated from at least 1 examiner site (gloves, gowns, or stethoscope) in 33 (67%) of 49 cases. Gloves were contaminated in 63%, gowns in 37%, and stethoscopes in 31%. All three items were positive for VRE in 24%. One case each had stethoscope and gown contamination without glove contamination. Only 1 (2%) of 49 stethoscopes was positive after wiping with an alcohol swab. Contamination at any site was more likely when the patient had a colostomy or ileostomy. Patients identified by rectal-swab culture alone were as likely to contaminate their examiners as were those identified by clinical specimens.Conclusions:Our study revealed a high rate of examiner contamination with VRE. The similar risk of contamination identified by surveillance and clinical cases reinforces concerns that patients not known to be colonized with VRE could serve as sources for dissemination. Wiping with alcohol is effective in decontaminating stethoscopes.

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