Control of Listeria monocytogenes on Turkey Frankfurters by Generally-Recognized-as-Safe Preservatives

2002 ◽  
Vol 65 (9) ◽  
pp. 1411-1416 ◽  
Author(s):  
MAHBUB ISLAM ◽  
JINRU CHEN ◽  
MICHAEL P. DOYLE ◽  
MANJEET CHINNAN
Keyword(s):  
Listeria Monocytogenes ◽  
Growth Inhibition ◽  
Sodium Benzoate ◽  
Potassium Sorbate ◽  
Sodium Propionate ◽  
Sodium Diacetate

Generally-recognized-as-safe chemicals applied to the surfaces of turkey frankfurters were evaluated for their ability to reduce populations of or inhibit the growth of Listeria monocytogenes. Frankfurters were treated prior to inoculation by dipping for 1 min in a solution of one of four preservatives (sodium benzoate, sodium propionate, potassium sorbate, and sodium diacetate) at three different concentrations (15, 20, and 25% [wt/vol]), with <0.3% of the preservative being present for each frankfurter. Subsequently, 0.1 ml of a five-strain mixture of L. monocytogenes (106 CFU/ml) was used to surface inoculate each frankfurter separately in a sterile stomacher bag. Inoculated frankfurter bags were held at 4, 13, and 22°C, and L. monocytogenes cells were enumerated at 0, 3, 7, 10, and 14 days of storage. The results of this study revealed that at all three concentrations of all four preservatives, the initial populations of L. monocytogenes decreased immediately by 1 to 2 log10 CFU/g. After 14 days of storage at 4°C, L. monocytogenes counts for all treated frankfurters were 3 to 4 log10 CFU/g less than those for the untreated frankfurters. After 14 days of storage at 13°C, L. monocytogenes counts for frankfurters treated with 25% sodium benzoate or 25% sodium diacetate were 3.5 to 4.5 log10 CFU/g less than those for untreated frankfurters, and those for frankfurters treated with 25% sodium propionate or 25% potassium sorbate were 2.5 log10 CFU/g less than those for untreated frankfurters. In all instances, the degree of growth inhibition was directly proportional to the concentration of the preservative. Only frankfurters treated with 25% sodium diacetate or sodium benzoate were significantly inhibitory to L. monocytogenes when held at 22°C for 7 days or longer. Interestingly, the untreated frankfurters held at 22°C were spoiled within 7 days, with copious slime formation, whereas there was no evidence of slime on any treated frankfurters after 14 days of storage.

Effect of Selected Generally Recognized as Safe Preservative Sprays on Growth of Listeria monocytogenes on Chicken Luncheon Meat

2002 ◽  
Vol 65 (5) ◽  
pp. 794-798 ◽  
Author(s):  
MAHBUB ISLAM ◽  
JINRU CHEN ◽  
MICHAEL P. DOYLE ◽  
MANJEET CHINNAN
Keyword(s):  
Listeria Monocytogenes ◽  
Sodium Benzoate ◽  
Untreated Control ◽  
Potassium Sorbate ◽  
Sodium Propionate ◽  
Luncheon Meat ◽  
Chemical Preservatives ◽  
Sodium Diacetate

The ability of selected generally recognized as safe (GRAS) chemical preservatives to reduce populations or inhibit growth of Listeria monocytogenes on chicken luncheon meat was evaluated. Slices of luncheon meat were treated by evenly spraying onto their surfaces 0.2 ml of a solution of one of four preservatives (sodium benzoate, sodium propionate, potassium sorbate, and sodium diacetate) at one of three different concentrations (15, 20, or 25% [wt/vol]). Each slice was then surface inoculated with a five-strain mixture of 105 CFU of L. monocytogenes per ml, held at 4, 13, or 22°C, and assayed for L. monocytogenes immediately after inoculation and at 3, 7, 10, and 14 days of storage. Initial reductions of L. monocytogenes populations ranged from 0.78 to 1.32 log10 CFU g−1 at day 0 for sodium benzoate– or sodium diacetate–treated meat, whereas reductions for the sodium propionate or potassium sorbate treatments were only 0.14 to 0.36 log10 CFU g−1. After 14 days of storage at 4°C, L. monocytogenes populations on all treated slices were 1.5 to 3 log10 CFU g−1 less than on the untreated slices. At 13°C and after 14 days of storage, L. monocytogenes populations were 3.5 and 5.2 log10 CFU g−1 less on luncheon meat slices treated with 25% sodium benzoate or 25% sodium diacetate, respectively, and ca. 2 log10 CFU g−1 less when treated with 25% sodium propionate or 25% potassium sorbate than on untreated control slices. Only sodium diacetate was highly inhibitory to L. monocytogenes on meat slices held at 22°C for 7 days or longer. Untreated luncheon meat held at 22°C was visibly spoiled within 10 days, whereas there was no evidence of visible spoilage on any treated luncheon meat at 14 days of storage.

Controlling Listeria monocytogenes on Sliced Ham and Turkey Products Using Benzoate, Propionate, and Sorbate

2007 ◽  
Vol 70 (10) ◽  
pp. 2306-2312 ◽  
Author(s):  
KATHLEEN A. GLASS ◽  
LINDSEY M. MCDONNELL ◽  
ROB C. RASSEL ◽  
KRISTINE L. ZIERKE
Keyword(s):  
Listeria Monocytogenes ◽  
Sodium Benzoate ◽  
Sodium Lactate ◽  
Product Type ◽  
Potassium Sorbate ◽  
Sodium Propionate ◽  
Significant Growth ◽  
Sodium Diacetate

The objective of this study was to identify concentrations of sorbate, benzoate, and propionate that prevent the growth of Listeria monocytogenes on sliced, cooked, uncured turkey breast and cured ham. Sixteen test formulations plus a control formulation for each product type were manufactured to include potassium sorbate, sodium benzoate, or sodium propionate, used alone and combined (up to 0.3% [wt/wt]), or with sodium lactate–sodium diacetate combinations. Products were inoculated with L. monocytogenes (5 log CFU/100-g package) and stored at 4, 7, or 10°C for up to 12 weeks, and triplicate samples per treatment were assayed biweekly by plating on modified Oxford agar. Data showed that 0.1% benzoate, 0.2% propionate, 0.3% sorbate, or a combination of 1.6% lactate with 0.1% diacetate prevented the growth of L. monocytogenes on ham stored at 4°C for 12 weeks, compared with greater than a 1-log increase at 4 weeks for the control ham without antimicrobials. When no nitrite was included in the formulation, 0.2% propionate used alone, a combination of 0.1% propionate with 0.1% sorbate, or a combination of 3.2% lactate with 0.2% diacetate was required to prevent listerial growth on the product stored at 4°C for 12 weeks. Inhibition was less pronounced when formulations were stored at abuse temperatures. When stored at 7°C, select treatments delayed listerial growth for 4 weeks but supported significant growth at 8 weeks. All treatments supported more than a 1-log increase in listerial populations when stored at 10°C for 4 weeks. These results verify that antimycotic agents inhibit the growth of L. monocytogenes on ready-to-eat meats but are more effective when used in combination with nitrite.

Inhibition of Listeria monocytogenes in Turkey and Pork-Beef Bologna by Combinations of Sorbate, Benzoate, and Propionate

2007 ◽  
Vol 70 (1) ◽  
pp. 214-217 ◽  
Author(s):  
KATHLEEN GLASS ◽  
DAWN PRESTON ◽  
JEFFREY VEESENMEYER
Keyword(s):  
Growth Rate ◽  
Listeria Monocytogenes ◽  
Sodium Benzoate ◽  
Antimicrobial Agents ◽  
Storage Period ◽  
Potassium Sorbate ◽  
Internal Temperature ◽  
High Moisture ◽  
Sodium Propionate ◽  
Low Concentrations

The control of Listeria monocytogenes was evaluated with ready-to-eat uncured turkey and cured pork-beef bologna with combinations of benzoate, propionate, and sorbate. Three treatments of each product type were formulated to include control with no antimycotic agents; a combination of 0.05% sodium benzoate and 0.05% sodium propionate; and a combination of 0.05% sodium benzoate and 0.05% potassium sorbate. Ingredients were mixed, stuffed into fibrous, moisture-impermeable casings, cooked to an internal temperature of 73.9°C, chilled, and sliced. The final product was surface inoculated with L. monocytogenes (4 log CFU per package), vacuum packaged, and stored at 4°C for 13 weeks. The antimycotic addition to the second and third uncured turkey treatments initially slowed the pathogen growth rate compared with the control, but populations of L. monocytogenes increased 5 log or more by 6 weeks. In contrast, the addition of antimycotic combinations in the cured bologna prevented growth of L. monocytogenes during the 13-week storage period at 4°C, compared with a more than 3.5-log increase in listerial populations in the control bologna, to which no antimicrobial agents had been added. These data suggest that low concentrations of antimycotic agents can prevent L. monocytogenes growth in certain ready-to-eat meats. Additional research is needed to define the levels needed to prevent growth of L. monocytogenes in high-moisture cured and uncured ready-to-eat meat and poultry and for gaining governmental approval for their use in such formulations.

Effect of Preservatives on Shiga Toxigenic Phages and Shiga Toxin of Escherichia coli O157:H7

2012 ◽  
Vol 75 (5) ◽  
pp. 959-965 ◽  
Author(s):  
TOMÁS SUBILS ◽  
VIRGINIA AQUILI ◽  
GUILLERMO EBNER ◽  
CLAUDIA BALAGUÉ
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Sodium Benzoate ◽  
Vero Cells ◽  
Lytic Activity ◽  
Potassium Sorbate ◽  
Lambda Phage ◽  
Sodium Propionate ◽  
Food Preservatives ◽  
Shiga Toxins

Toxin synthesis by Shiga toxin–producing Escherichia coli (STEC) appears to be coregulated through the induction of the integrated bacteriophages that encode the toxin genes. These phages might be the principal means for the dissemination and release of Shiga toxins. We evaluated the effect of three common food preservatives, potassium sorbate, sodium benzoate, and sodium propionate, on the propagation of the phages and Shiga toxins. We tested each preservative at four concentrations, 1, 1.25, 2.5, and 5 mg/ml, both on free phages and on lysogenic phages in bacteria. We also evaluated the expression of a lambdoid phage, which was exposed to increasing concentrations of preservatives, by measuring β-galactosidase activity from SPC105, a transductant strain. Furthermore, we tested the effect of the preservatives on cytotoxigenic activity of Shiga toxin on Vero cells. We detected an increase of the inhibitory effect of the phage lytic activity, both in lysogenic and free phages, as the preservative concentration increased. However, the inhibition was higher on the lysogenic phages release than on free phages. Sodium benzoate and potassium sorbate were about equal at inhibiting phages; they were more effective than sodium propionate. A significant decrease of lacZ expression, encoded in a lambda phage, was observed. We also found a reduction in Shiga toxin titer caused by exposure of E. coli O157:H7 to 5 mg/ml sodium benzoate or potassium sorbate. These results imply that these three preservatives, used to inhibit microbial spoilage of foods, also act to inhibit lytic activity and dispersion of a phage carrying the gene encoding powerful Shiga cytotoxins. Also notable was the inactivation of Shiga toxin activity, although this effect was detected using concentrations of preservatives greater than those allowed by the Argentine Food Code.

Inhibition of Listeria monocytogenes Growth in Cured Ready-to-Eat Meat Products by Use of Sodium Benzoate and Sodium Diacetate

2008 ◽  
Vol 71 (7) ◽  
pp. 1386-1392 ◽  
Author(s):  
D. L. SEMAN ◽  
S. C. QUICKERT ◽  
A. C. BORGER ◽  
J. D. MEYER
Keyword(s):  
Listeria Monocytogenes ◽  
Sodium Benzoate ◽  
Meat Products ◽  
Regression Method ◽  
Time To Failure ◽  
High Moisture ◽  
Current Standard ◽  
Sodium Diacetate ◽  
Main Effects ◽  
Central Composite

The effect of sodium benzoate (0.08 to 0.25%) in combination with different concentrations of sodium diacetate (0.05 to 0.15%) and NaCl (0.8 to 2%) and different finished product moisture (55 to 75%) on the growth of Listeria monocytogenes in ready-to-eat meat products was evaluated using a central composite design over 18 weeks of storage at 4°C. The effects of these factors on time to growth were analyzed using a time-to-failure regression method. All main effects were significant except product moisture, which was significant when included in the two- and three-way interactions (P < 0.05). Sodium benzoate was more effective (lengthening time to growth) when used with increasing concentrations of sodium diacetate and salt and decreasing finished product moisture. The model indicated that low-moisture products, e.g., bologna or wieners, could have time-to-growth values longer than 18 weeks if they were formulated with 0.1% sodium benzoate and 0.1% sodium diacetate. Time to growth in high-moisture products, e.g., ham or cured turkey breast at 75% moisture, was predicted to be much shorter for the same basic formulation (0.1% sodium benzoate and 0.1% sodium diacetate). Consequently, high-moisture ready-to-eat products in which sodium benzoate is limited to 0.1% (current standard for generally recognized as safe) may need additional ingredients to effectively inhibit growth of L. monocytogenes.

Organic Acids and Their Salts as Dipping Solutions To Control Listeria monocytogenes Inoculated following Processing of Sliced Pork Bologna Stored at 4°C in Vacuum Packages

2001 ◽  
Vol 64 (11) ◽  
pp. 1722-1729 ◽  
Author(s):  
JOHN SAMELIS ◽  
JOHN N. SOFOS ◽  
MINDY L. KAIN ◽  
JOHN A. SCANGA ◽  
KEITH E. BELK ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Listeria Monocytogenes ◽  
Organic Acids ◽  
Safety Issue ◽  
Sodium Lactate ◽  
Commercial Application ◽  
Potassium Sorbate ◽  
Cured Meats ◽  
Potassium Benzoate ◽  
Sodium Diacetate

Postprocessing contamination of cured meats with Listeria monocytogenes has become a major concern for the meat processing industry and an important food safety issue. This study evaluated aqueous dipping solutions of organic acids (2.5 or 5% lactic or acetic acid) or salts (2.5 or 5% sodium acetate or sodium diacetate, 5 or 10% sodium lactate, 5% potassium sorbate or potassium benzoate) to control L. monocytogenes on sliced, vacuum-packaged bologna stored at 4°C for up to 120 days. Organic acids and salts were applied by immersing (1 min) in each solution inoculated (102 to 103 CFU/cm2) slices of bologna before vacuum packaging. Growth of L. monocytogenes (PALCAM agar) on inoculated bologna slices without treatment exceeded 7 log CFU/cm2 (P < 0.05) at 20 days of storage. No significant (P > 0.05) increase in L. monocytogenes populations occurred on bologna slices treated with 2.5 or 5% acetic acid, 5% sodium diacetate, or 5% potassium benzoate from day 0 to 120. Products treated with 5% potassium sorbate and 5% lactic acid were stored for 50 and 90 days, respectively, before a significant (P < 0.05) increase in L. monocytogenes occurred. All other treatments permitted growth of the pathogen at earlier days of storage, with sodium lactate (5 or 10%) permitting growth within 20 to 35 days. Extent of bacterial growth on trypticase soy agar plus 0.6% yeast extract (TSAYE) was similar to that on PALCAM, indicating that the major part of total bacteria grown on TSAYE agar plates incubated at 30°C was L. monocytogenes. Further studies are needed to evaluate organic acids and salts as dipping solutions at abusive temperatures of retail storage, to optimize their concentrations in terms of product sensory quality, and to evaluate their effects against various other types of microorganisms and on product shelf life. In addition, technologies for the commercial application of postprocessing antimicrobial solutions in meat plants need to be developed.

scholarly journals Quantitative Analysis of Food Additives in a Beverage using High Performance Liquid Chromatography and Diode Array Detection Coupled with Chemometrics

2020 ◽  
Vol 103 (3) ◽  
pp. 779-783
Author(s):  
Özlem Aksu Dönmez ◽  
Şule Dinç-Zor ◽  
Bürge Aşçı ◽  
Abdürrezzak E Bozdoğan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sodium Benzoate ◽  
High Performance ◽  
Food Additives ◽  
Potassium Sorbate ◽  
Diode Array ◽  
Diode Array Detection ◽  
Ponceau 4R ◽  
Array Detection

Abstract Background In many countries, the levels of synthetic food additives causing harm to humans have been determined and their use has been controlled by legal regulations. Sensitive, accurate and low-cost analysis methods are required for food additive determination. Objective In this study, a fast high performance liquid chromatography-diode array detection (HPLC-DAD) analytical methodology for quantification of sodium benzoate, potassium sorbate, ponceau 4R, and carmoisine in a beverage was proposed. Methods Partial least squares (PLS) and principal component regression (PCR) multivariate calibration methods applied to chromatograms with overlapped peaks were used to establish a green and smart method with short isocratic elution. A series of synthetic solutions including different concentrations of analytes were used to test the prediction ability of the developed methods. Conclusions The average recoveries for all target analytes were in the range of 98.27–101.37% with average relative prediction errors of less than 3%. The proposed chemometrics-assisted HPLC-DAD methods were implemented to a beverage successfully. Analysis results from sodium benzoate, potassium sorbate, ponceau 4R, and carmoisine in a beverage by PLS-2 and PCR were statistically compared with conventional HPLC. Highlights The HPLC methods coupled with the PLS-2 and PCR algorithm could provide a simple, quick and accurate strategy for simultaneous determination of sodium benzoate, potassium sorbate, ponceau 4R, and carmoisine in a beverage sample.

scholarly journals Resistance of Yeast Flora of Labaneh to Potassium Sorbate and Sodium Benzoate

1997 ◽  
Vol 80 (10) ◽  
pp. 2304-2309 ◽  
Author(s):  
Ghadeer F. Mihyar ◽  
Mohammed I. Yamani ◽  
Ali K. Al-Sa’ed
Keyword(s):  
Sodium Benzoate ◽  
Potassium Sorbate ◽  
Yeast Flora

Modeling the Growth Boundary of Listeria monocytogenes in Ready-to-Eat Cooked Meat Products as a Function of the Product Salt, Moisture, Potassium Lactate, and Sodium Diacetate Concentrations

2004 ◽  
Vol 67 (10) ◽  
pp. 2195-2204 ◽  
Author(s):  
J. D. LEGAN ◽  
D. L. SEMAN ◽  
A. L. MILKOWSKI ◽  
J. A. HIRSCHEY ◽  
M. H. VANDEVEN
Keyword(s):  
Listeria Monocytogenes ◽  
Meat Products ◽  
Growth Conditions ◽  
Continuous Variables ◽  
Surface Design ◽  
Potassium Lactate ◽  
Contour Plots ◽  
Sodium Diacetate ◽  
Factor Interactions ◽  
Composite Response

A central composite response surface design was used to determine the time to growth of Listeria monocytogenes as a function of four continuous variables: added sodium chloride (0.8 to 3.6%), sodium diacetate (0 to 0.2%), potassium lactate syrup (60% [wt/wt]; 0.25 to 9.25%), and finished-product moisture (45.5 to 83.5%) in ready-to-eat cured meat products. The design was repeated for ready-to-eat uncured meat products giving a fifth categorical variable for cure status. Products were stored at 4°C. The results were modeled using a generalized regression approach. All five main effects, six two-factor interactions, and two quadratic terms were statistically significant. The model was used to show the boundary between growth and no-growth conditions at 4°C using contour plots of time to growth. It was validated using independent challenge studies of cured and uncured products. Generally, the model predicted well, particularly for cured products, where it will be useful for establishing conditions that prevent the growth of L. monocytogenes. For uncured products, there was good agreement overall between predicted and observed times to growth, but the model is less thoroughly validated than for cured products. The model should initially only be used for screening of formulations likely to prevent growth of Listeria monocytogenes in uncured products, with recommendations subject to confirmation by challenge studies.

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