Determination of Bisphenol A in Milk and Dairy Products by High-Performance Liquid Chromatography with Fluorescence Detection

2003 ◽  
Vol 66 (8) ◽  
pp. 1439-1443 ◽  
Author(s):  
JEONG-HUN KANG ◽  
FUSAO KONDO
Keyword(s):  
Bisphenol A ◽  
Dairy Products ◽  
High Performance ◽  
Solid Phase ◽  
Skim Milk ◽  
The European Union ◽  
Condensed Milk ◽  
Oasis Hlb ◽  
Milk And Dairy Products

This study was conducted to develop a selective and sensitive method for the determination of bisphenol A (BPA) levels in milk and dairy products. A method based on solvent extraction with acetonitrile and solid-phase extraction (SPE) was developed for the analysis of BPA in milk, yogurt, cream, butter, pudding, condensed milk, and flavored milk, and a method using two SPE cartridges (OASIS HLB and Florisil cartridge) for skim milk was also developed. The developed methods showed good recovery levels (77 to 102%) together with low detection limits (1 μg/liter for milk, yogurt, pudding, condensed milk, flavored milk, and skim milk and 3 μg/liter for cream and butter). These methods are simple, sensitive, and suitable for the analysis of BPA in milk and dairy products. When 40 milk and dairy products were analyzed by the proposed methods, BPA was not identified in noncanned products, but its levels ranged from 21 to 43 μg/kg in canned products, levels that were 60- to 140-fold lower than the migration limits in the European Union and Japan.

CONTROL PROBLEMS AND METHODS FOR DETECTING RESIDUAL AMOUNTS OF ANTIBACTERIAL SUBSTANCES IN MILK AND DAIRY PRODUCTS

2020 ◽  
Vol 1 (2) ◽  
pp. 165-169
Author(s):  
G.M. Goryainova ◽  
◽  
E.A. Denisova ◽  
L.V. Arsen`eva ◽  
V.S. Babunova ◽  
...  
Keyword(s):  
Dairy Products ◽  
High Performance ◽  
Raw Milk ◽  
Detection Methods ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Official Method ◽  
The European Union ◽  
Residual Amount ◽  
Milk And Dairy Products

The article is devoted to the study of control issues and methods for detecting residual amounts of antibacterial substances in milk and dairy products. The presence of a large number of antibacterial drugs on the Russian market and their use in dairy farming makes it an urgent problem to detect their residual amount, both in raw milk and in dairy products. The lack of standardized detection methods, sufficiently equipped and accredited laboratories, and the high cost and duration of research are obstacles to monitoringthe content of the residual amount of antibiotics., including state control. Currently, the state monitoring system for screening antibiotics uses the enzyme-linked immunosorbent assay method (ELISA), which is the official method for monitoring animal products adopted in the European Union. To confirm the quantitative content of antibiotics, a high-performance liquid chromatography (HPLC) method with various types of detectors is used, which is reliable but very time-consuming for screening a large number of samples. Using the patented Randox Biochip technology as a method for detecting the residual amount of antibiotics in milk and dairy products, it would be possible to determine up to 25 antibacterial substances in one sample, including those that are not often used in veterinary practice.

scholarly journals Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

2020 ◽  
pp. 150-157
Author(s):  
S. Senin ◽  
V. Danchuk ◽  
S. Midyk ◽  
V. Ushkalov ◽  
O. Iakubchak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Analytical Methods ◽  
Dairy Products ◽  
High Performance ◽  
Raw Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk And Dairy Products

The dairy industry of Ukraine is developing dynamically, its needs for the quality of raw materials are growing significantly. Detection of mycotoxins in raw milk is one of the main indicators of its safety. The high degree of toxicity of mycotoxins is a threat to the health of the lactating animal, so a large number of them are excreted in milk. If we talk about ruminants, the vast majority of mycotoxins are utilized by microorganisms of the pancreas, which does not occur in monogastric animals, so the list of mycotoxins in their milk can be much wider than the secretion of mammalian mammals. To date, the maximum permissible levels (MRLs) of mycotoxins in raw milk and dairy products have been established. Thus, a comprehensive determination of the content of mycotoxins in the secretion of the breast has not only technological but also important diagnostic value. Milk sample preparation is the most important step in the determination of mycotoxins and consists of sampling, extraction and purification from impurities. For the extraction of aflatoxins, the method of liquid extraction with acetonitrile or chloroform is used. Purification of extracts is carried out on immunoaffinity columns, cartridges with special sorbents or using certain manufacturers (MycoSep®).Enzyme-linked immunosorbent assay and high-performance liquid chromatography with fluorescence detection are used to determine aflatoxin B1 and M1 in raw milk of cows. However, all these methods have a number of disadvantages, namely: long and expensive sample preparation and insufficiently high selectivity. Currently, the complex determination of mycotoxins in various matrices by high-performance liquid chromatography with mass spectrometric detection (LC-MS/MS) and the use of modified QuEChERS sample preparation is gaining popularity. The advantage of this technique is the combination of faster and cheaper sample preparation of QuEChERS samples with highly selective LC-MS/MS chromatography. Key words: mycotoxins, raw milk, analytical methods, QuEChERS.

scholarly journals Determination of melamine in milk and dairy products by high performance liquid chromatography

2012 ◽  
Vol 95 (2) ◽  
pp. 602-608 ◽  
Author(s):  
A. Filazi ◽  
U.T. Sireli ◽  
H. Ekici ◽  
H.Y. Can ◽  
A. Karagoz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dairy Products ◽  
High Performance ◽  
Milk And Dairy Products
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