Optimal control for dengue eradication program under the media awareness effect

In this article, a mathematical model is proposed to assess the effects of media awareness on dengue eradication programs. First, the existence and local stability of equilibrium points are discussed using the concept of the basic reproduction number. Using the center-manifold theorem, it is shown that the proposed model always undergoes a forward bifurcation at the basic reproduction number equal to unity. It is observed that the high-intensity media awareness could reduce the size of the endemic equilibrium. Based on local sensitivity analysis, we identify the three most sensitive parameters, namely the natural death rate of mosquito (μ v ), infection rates (β h1, β v1), and hospitalization rate (η). Hence, control variables need to be introduced to increase/reduce these parameters. In this article, we use three different control variables, namely the media campaign, (u 1(t)), to reduce infection rates, additional hospitalization rate, (u 2(t)), and fumigation rate, (u 3(t)), to increase mosquitoes death rate. Pontryagin’s maximum principle is used to determine the optimal conditions. Some numerical simulations are performed to describe a possible scenario in the field. Cost effectiveness analysis is then conducted to determine the best strategy for the dengue eradication program. We conclude that a combination of media campaigns and fumigation is the most effective strategy to prevent a significant increase in the number of infected individuals.

EFFECTIVITY OF ILLEGAL, UNREPORTED, UNREGULATED FISHING (IUUF) ERADICATION PROGRAM TOWARD LEGAL, REPORTED, REGULATED FISHING (LRRF)

Artikel ini bertujuan untuk menganalisis efektivitas kinerja implementasi pemberantasan Illegal, Unreported, Unregulated Fishing (IUUF) menuju Legal, Reported, Regulated Fishing (LRRF) di Indonesia. Potensi sumber daya ikan sebesar 1.772 triliun terancam musnah dengan masifnya IUUF. Pemberantasan IUUF telah dilaksanakan secara intensif mulai dari tahun 2015. Akan tetapi, IUUF masih masif di wilayah pengelolaan perikanan Negara Republik Indonesia. Metode penelitian dilakukan dengan studi literatur, temuan data-data sekunder, wawancara dilakukan dengan snowball kepada narasumber dan informan pemilik otoritas dan praktisi yang terkait dengan praktek pemberantasan IUUF. Hasil penelitian pemberantasan IUUF melalui pemberian sanksi penenggelaman efektif membuat terapi kejut tapi tidak menimbulkan efek jera, internalisasi penyadartahuan kepada nelayan dan pelaku usaha, penguatan sarana dan prasarana pengawasan, dan penguatan peran Indonesia di Kawasan ASEAN diprediksi efektif dalam mengatasi IUUF menjadi LRRF. Kata kunci: Efektivitas, IUUF, LRRF

Exchange of C-Terminal Variable Sequences within Morbillivirus Nucleocapsid Protein Are Tolerated: Development and Evaluation of Two Marker (DIVA) Vaccines (Sungri/96 DIVA, Nigeria/75/1 DIVA) against PPR

Across Africa, the Middle East, and Asia, peste des petits ruminants virus (PPRV) places a huge disease burden on agriculture, affecting, in particular, small ruminant production. The recent PPR outbreaks in Northern Africa, the European part of Turkey, and Bulgaria represent a significant threat to mainland Europe, as a source of disease. Although two safe and efficacious live attenuated vaccines (Sungri/96 and Nigeria/75/1) are available for the control of PPR, current serological tests do not enable the differentiation between naturally infected and vaccinated animals (DIVA). The vaccinated animals develop a full range of immune responses to viral proteins and, therefore, cannot be distinguished serologically from those that have recovered from a natural infection. This poses a serious problem for the post-vaccinal sero-surveillance during the ongoing PPR eradication program. Furthermore, during the latter stages of any eradication program, vaccination is only possible if the vaccine used is fully DIVA compliant. Using reverse genetics, we have developed two live attenuated PPR DIVA vaccines (Sungri/96 DIVA and Nigeria/75/1 DIVA), in which the C-terminal variable region of the PPRV N-protein has been replaced with dolphin morbillivirus (DMV). As a proof of principle, both the DIVA vaccines were evaluated in goats in pilot studies for safety and efficacy, and all the animals were clinically protected against the intranasal virulent virus challenge, similar to the parent vaccines. Furthermore, it is possible to differentiate between infected animals and vaccinated animals using two newly developed ELISAs. Therefore, these DIVA vaccines and associated tests can facilitate the sero-monitoring process and speed up the implementation of global PPR eradication through vaccination.

Benefit of Bovine Viral Diarrhoea (BVD) Eradication in Cattle on Pestivirus Seroprevalence in Sheep

Bovine viral diarrhoea virus (BVDV) and Border disease virus (BDV) are closely related pestiviruses of cattle and sheep, respectively. Both viruses may be transmitted between either species, but control programs are restricted to BVDV in cattle. In 2008, a program to eradicate bovine viral diarrhoea (BVD) in cattle was started in Switzerland. As vaccination is prohibited, the cattle population is now widely naïve to pestivirus infections. In a recent study, we determined that nearly 10% of cattle are positive for antibodies to BDV. Here, we show that despite this regular transmission of BDV from small ruminants to cattle, we could only identify 25 cattle that were persistently infected with BDV during the last 12 years of the eradication program. In addition, by determining the BVDV and BDV seroprevalence in sheep in Central Switzerland before and after the start of the eradication, we provide evidence that BVDV is transmitted from cattle to sheep, and that the BVDV seroprevalence in sheep significantly decreased after its eradication in cattle. While BDV remains endemic in sheep, the population thus profited at least partially from BVD eradication in cattle. Importantly, on a national level, BVD eradication does not appear to be generally derailed by the presence of pestiviruses in sheep. However, with every single virus-positive cow, it is necessary to consider small ruminants as a potential source of infection, resulting in costly but essential investigations in the final stages of the eradication program.

Risk Factors Associated with the Alpine Multispecies Farming System in the Eradication of CAEV in South Tyrol, Italy

South Tyrol has implemented, in 2007, a mandatory eradication program against Caprine Arthritis Encephalitis Virus (CAEV), a virus known to cause economic losses related to decreases in milk production and milk quality in goats, along with poor animal welfare and premature death. After a great initial decrease in the seroprevalence, the program has reached a tailing phase with scattered positivities. Potential risk factors associated with the multispecies farming system, a traditional approach in South Tyrol, are evaluated in this study, in order to better understand some of the potential causes leading to the tailing phenomenon. A statistically significant number of farms was selected for the present study, based on the risk factors evaluated. Even though there is no statistically significant association between the practices evaluated and the incidence of infection, the authors believe that it is important to highlight potential risks that may threaten the outcome of this eradication program.

Alleles and algorithms: The role of genetic analyses and remote sensing technology in an ant eradication program

Eradication programs for invasive ants are often hampered by a lack of effective tools to detect, contain and kill the pests. Among the range of tools employed in the course of a 20-year eradication program for red imported fire ant, Solenopsis invicta, in Australia, two of the most crucial for success are genetic analysis at both individual colony and population scales, and remote sensing for the detection of S. invicta mounds over large areas. Several genetic analyses are used by the program as an everyday operational tool to guide the eradication effort; for example, genotyping of the social form determines where and how far we need to search and treat, whereas nest relatedness derived from microsatellites aids in deciding when and where to target investigations into human-assisted movement of the pest. Microsatellite genotyping can determine the origin of new invasions into the country and has been used to verify the eradication of six distinct incursions of S. invicta in Australia, as well as demonstrating the pressure being exerted on the remaining Queensland population by the current eradication activities. Remote sensing played a key role in delimiting the extent of the S. invicta infestation in southeast Queensland in 2015, and in the future will assist in both delimitation and in verifying eradication of this ant in treatment areas as part of the proof of freedom process. Unquestionably, without these tools, the battle to eradicate S. invicta from Australia would be severely constrained, if not lost. These technologies may be applicable in management or eradication programs for S. invicta worldwide, and potentially for other invasive ant species.

Successful eradication of Mycoplasma hyopneumoniae from the Norwegian pig population – 10 years later

Background Mycoplasma hyopneumoniae (Mhyo) is the causative agent of enzootic pneumonia in pigs which adversely affects animal health and welfare, in addition to causing considerable economical losses. This paper presents the implementation of the national Mhyo eradication program in Norway, the subsequent population wide surveillance and documentation on the current freedom from Mhyo in the Norwegian pig population. In 1994, the Board of The Norwegian Pig Health Service decided on conducting a national surveillance and eradication program for Mhyo. The program aimed for population wide freedom from Mhyo, based on serological surveillance. A partial depopulation program was initiated in all Mhyo positive farrow-to-feed and farrow-to-finish herds. Total depopulation was performed in all positive finisher herds. Results From 1994 to 2009, a total of 138,635 pigs in 3211 herds were serologically tested for the presence of antibodies against Mhyo. Of these, 5538 (4%) individual samples and 398 (12.4%) of the herds were defined as positive. In 2009, the Norwegian pig population was declared free from Mhyo, and has been so since then. From 2009 through 2019, a total of 44,228 individual serum samples have been analyzed for the presence of antibodies against Mhyo and found negative in the National surveillance program. Conclusion Eradication of Mhyo infections has resulted in improved health and welfare of the Norwegian pig population. The success of the strategy is based on numerous factors, such as moderate to low prevalence of the agent, well documented and effective eradication protocols, accurate diagnostic tests, relatively small herds, low herd density in most parts of the country and negligible import of live pigs. In addition, economic benefit due to a premium on pigs marketed from herds free from Mhyo, a well-structured commercial pig population, and finally, the loyalty and significant effort of farmers, abattoir employees and veterinarians were crucial factors. To maintain the infection-free status at national level, a continuous alertness is required in the future to discover possible Mhyo infections and ensure rapid sampling and diagnostics. Any findings of Mhyo positive pig herds in Norway will result in immediate eradication.

Stage-specific overcompensation, the hydra effect, and the failure to eradicate an invasive predator

As biological invasions continue to increase globally, eradication programs have been undertaken at significant cost, often without consideration of relevant ecological theory. Theoretical fisheries models have shown that harvest can actually increase the equilibrium size of a population, and uncontrolled studies and anecdotal reports have documented population increases in response to invasive species removal (akin to fisheries harvest). Both findings may be driven by high levels of juvenile survival associated with low adult abundance, often referred to as overcompensation. Here we show that in a coastal marine ecosystem, an eradication program resulted in stage-specific overcompensation and a 30-fold, single-year increase in the population of an introduced predator. Data collected concurrently from four adjacent regional bays without eradication efforts showed no similar population increase, indicating a local and not a regional increase. Specifically, the eradication program had inadvertently reduced the control of recruitment by adults via cannibalism, thereby facilitating the population explosion. Mesocosm experiments confirmed that adult cannibalism of recruits was size-dependent and could control recruitment. Genomic data show substantial isolation of this population and implicate internal population dynamics for the increase, rather than recruitment from other locations. More broadly, this controlled experimental demonstration of stage-specific overcompensation in an aquatic system provides an important cautionary message for eradication efforts of species with limited connectivity and similar life histories.