the dosages used to kill mosquitoes, non-target organisms are safe. Both of these products, especially Bacillus, break down quickly and should therefore present no hazard to water quality. Given that a site such as Big Bay may become a mecca for those wishing to swim, sail or even fish, a surveillance programme and some environmental modifications are necessary. The deeper open waters of this bay coupled with a vegetation-free foreshore as a buffer zone, perhaps 400 m on either side of a swimming zone, should minimize or even negate swimmer’s itch. Adjacent Hydrilla and other macrophytes will require clearing as these will also present a physical hazard to swimmers and watercraft. The monitoring programmes could ideally be done three to four weeks prior to extensive public usage to allow time for any remedial action. The prevalence of key mosquito species and of large Austropeplea (and Gyraulus and Amerianna) snails can be established quickly as can cercarial infection in the snails. If it is found necessary to establish infection rates in mosquitoes, the newly developed Ross River and Barmah Forest virus testing procedures using mosquito cell cultures and enzyme immunosorbent assay (Oliveira et al. 1995) would require six days processing time. This offers considerable economy over previous methods using intracerebral inoculation of baby suckling mice. We would suggest that prior to selected recreational events, especially those from March to May, the Water Supply Board should initiate the action shown in Figure 9.6. The information supplied in response to a request should be communicated to recreational users to ensure that they are aware of the risks. Perhaps mosquito, arbovirus, and schistosome status could be displayed in the same way as fire hazard status is commonly indicated. It would be remiss of us to generate the impression that we had all the answers to the Ross River dam. The stage 2A lake and its surrounds are undergoing a process of ecological change and realization of this must remain paramount. There are issues relating to mosquito biology and behaviour and to do with snail ecology generally that would repay further study. Thus further selective monitoring and research should not be forsaken. References

1998 ◽  
pp. 155-157
Keyword(s):  
Processing Time ◽  
Fire Hazard ◽  
Mosquito Species ◽  
Buffer Zone ◽  
Ecological Change ◽  
Testing Procedures ◽  
Selective Monitoring ◽  
Physical Hazard ◽  
A Site ◽  
Virus Testing

scholarly journals High-Throughput Sequencing Identifies Novel Viruses in Nectarine: Insights to the Etiology of Stem-Pitting Disease

2016 ◽  
Vol 106 (5) ◽  
pp. 519-527 ◽  
Author(s):  
D. E. V. Villamor ◽  
T. A. Mekuria ◽  
S. S. Pillai ◽  
K. C. Eastwell
Keyword(s):  
High Throughput ◽  
Pathogen Detection ◽  
High Throughput Sequencing ◽  
Prunus Persica ◽  
Disease Symptoms ◽  
Testing Procedures ◽  
Molecular Tests ◽  
Putative Member ◽  
Stem Pitting ◽  
Virus Testing

Recent studies have shown the superiority of high-throughput sequencing (HTS) technology over many standard protocols for pathogen detection. HTS was initiated on fruit tree accessions from disparate sources to improve and advance virus-testing procedures. A virus with genomic features resembling most closely that of the recently described Nectarine stem-pitting-associated virus, putative member of genus Luteovirus, was found in three nectarine trees (Prunus persica cv. nectarina), each exhibiting stem-pitting symptoms on the woody cylinder above the graft union. In these samples, HTS also revealed the presence of a coinfecting virus with genome characteristics typical of members of the genus Marafivirus. The same marafivirus- and luteovirus-like viruses were detected in nonsymptomatic nectarine and peach selections, indicating only a loose relationship between these two viruses with nectarine stem-pitting disease symptoms. Two selections infected with each of these viruses had previously tested free of known virus or virus-like agents using the current biological, serological, and molecular tests employed at the Clean Plant Center Northwest. Overall, this study presents the characterization by HTS of novel marafivirus- and luteovirus-like viruses of nectarine, and provides further insights into the etiology of nectarine stem-pitting disease. The discovery of these new viruses emphasizes the ability of HTS to reveal viruses that are not detected by existing protocols.

scholarly journals Introduction

Focaal ◽  
2014 ◽  
Vol 2014 (70) ◽  
pp. 3-11 ◽  
Author(s):  
Caroline Humphrey ◽  
Vera Skvirskaja
Keyword(s):  
Black Sea ◽  
Cultural Influences ◽  
Buffer Zone ◽  
The Black Sea ◽  
Area Studies ◽  
Political Crises ◽  
A Site

The introduction first outlines different perspectives on the Black Sea: in history, as a site of imperial conflicts and a buffer zone; in area studies, as a “region”; and in anthropology, as a sea crisscrossed by migration, cultural influences, alternative visions, and often a mutual turning of backs. We then discuss the Black Sea in the context of maritime ethnography and the study of ports, “hero cities”, pipelines, and political crises. The following sections consider Smith's notion of the “territorialization of memory” in relation to histories of exile and the more recent interactions brought about by migration and trade. In the concluding section we discuss how the Black Sea has appeared as a “horizon” and imaginary of the beyond for the peoples living around its shores.

scholarly journals Surveillance, insecticide resistance and control of an invasive Aedes aegypti (Diptera: Culicidae) population in California

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 194 ◽  
Author(s):  
Anthony J. Cornel ◽  
Jodi Holeman ◽  
Catelyn C. Nieman ◽  
Yoosook Lee ◽  
Charles Smith ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Mosquito Species ◽  
Residential Areas ◽  
Mosquito Abundance ◽  
New Locations ◽  
New Formulation ◽  
A Site ◽  
Bg Sentinel ◽  
And Control

The invasion and subsequent establishment in California of Aedes aegypti in 2013 has created new challenges for local mosquito abatement and vector control districts. Studies were undertaken to identify effective and economical strategies to monitor the abundance and spread of this mosquito species as well as for its control. Overall, BG Sentinel (BGS) traps were found to be the most sensitive trap type to measure abundance and spread into new locations. Autocidal-Gravid-Ovitraps (AGO-B), when placed at a site for a week, performed equally to BGS in detecting the presence of female Ae. aegypti. Considering operational cost and our findings, we recommend use of BGS traps for surveillance in response to service requests especially in locations outside the known infestation area. We recommend AGO-Bs be placed at fixed sites, cleared and processed once a week to monitor mosquito abundance within a known infestation area. Long-term high density placements of AGO-Bs were found to show promise as an environmentally friendly trap-kill control strategy. California Ae. aegypti were found to be homozygous for the V1016I mutation in the voltage gated sodium channel gene, which is implicated to be involved in insecticide resistance. This strain originating from Clovis, California was resistant to some pyrethroids but not to deltamethrin in bottle bio-assays. Sentinel cage ultra-low-volume (ULV) trials using a new formulation of deltamethrin (DeltaGard®) demonstrated that it provided some control (average of 56% death in sentinel cages in a 91.4 m spray swath) after a single truck mounted aerial ULV application in residential areas.

scholarly journals Evidence of circulation of Orthobunyaviruses in diverse mosquito species in Kwale County, Kenya

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Hellen Koka ◽  
Joel Lutomiah ◽  
Solomon Langat ◽  
Edith Koskei ◽  
Albert Nyunja ◽  
...  
Keyword(s):  
Mosquito Species ◽  
Febrile Illness ◽  
Vero Cells ◽  
Rural Site ◽  
Light Traps ◽  
Public Health Importance ◽  
Larval Survey ◽  
Monitoring And Surveillance ◽  
A Site ◽  
Bg Sentinel

Abstract Background Arbovirus surveillance and recurrence of outbreaks in Kenya continues to reveal the re-emergence of viruses of public health importance. This calls for sustained efforts in early detection and characterization of these agents to avert future potential outbreaks. Methods A larval survey was carried out in three different sites in Kwale County, Vanga, Jego and Lunga Lunga. All containers in every accessible household and compound were sampled for immature mosquitoes. In addition, adult mosquitoes were also sampled using CO2-baited CDC light traps and BG-Sentinel traps in the three sites and also in Tsuini. The mosquitoes were knocked down using trimethylamine and stored in a liquid nitrogen shipper for transportation to the laboratory where they were identified to species, pooled and homogenized ready for testing. Results A total of 366 houses and 1730 containers were inspected. The House Index (HI), Container Index (CI) and Breateau Index (BI) for Vanga Island were (3%: 0.66: 3.66) respectively. In Jego, a rural site, the HI, CI and BI were (2.4%: 0.48: 2.4) respectively. In Lunga Lunga, a site in an urban area, the HI, CI and BI were (22.03%: 3.97: 29.7) respectively. The indices suggest that this region is at risk of arbovirus transmission given they were above the WHO threshold (CI > 1, HI > 1% and BI > 5). The most productive containers were the concrete tanks (44.4%), plastic tank (22.2%), claypot (13.3%), plastic drums (8.9%), plastic basins (4%), jerricans (1.2%) and buckets (0.3%). Over 20,200 adult mosquitoes were collected using CDC light traps, and over 9,200 using BG- sentinel traps. These mosquitoes were screened for viruses by inoculating in Vero cells. Eleven Orthobunyavirus isolates were obtained from pools of Ae. pembaensis (4), Ae. tricholabis (1), Cx. quinquefasciatus (3), Culex spp. (1) and Cx. zombaensis (2). Five of the Orthobunyaviruses were sequenced and four of these were determined to be Bunyamwera viruses while one isolate was found to be Nyando virus. One isolate remained unidentified. Conclusions These results indicate circulation of Orthobunyaviruses known to cause diverse grades of febrile illness with rash in humans in this region and highlights the need for continued monitoring and surveillance to avert outbreaks.

scholarly journals Surveillance, insecticide resistance and control of an invasive Aedes aegypti (Diptera: Culicidae) population in California

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 194 ◽  
Author(s):  
Anthony J. Cornel ◽  
Jodi Holeman ◽  
Catelyn C. Nieman ◽  
Yoosook Lee ◽  
Charles Smith ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Mosquito Species ◽  
Residential Areas ◽  
Mosquito Abundance ◽  
New Locations ◽  
New Formulation ◽  
A Site ◽  
Bg Sentinel ◽  
And Control

The invasion and subsequent establishment in California of Aedes aegypti in 2013 has created new challenges for local mosquito abatement and vector control districts. Studies were undertaken to identify effective and economical strategies to monitor the abundance and spread of this mosquito species as well as for its control. Overall, BG Sentinel (BGS) traps were found to be the most sensitive trap type to measure abundance and spread into new locations. Autocidal-Gravid-Ovitraps (AGO-B), when placed at a site for a week, performed equally to BGS in detecting the presence of female Ae. aegypti. Considering operational cost and our findings, we recommend use of BGS traps for surveillance in response to service requests especially in locations outside the known infestation area. We recommend AGO-Bs be placed at fixed sites, cleared and processed once a week to monitor mosquito abundance within a known infestation area. Long-term high density placements of AGO-Bs were found to show promise as an environmentally friendly trap-kill control strategy. California Ae. aegypti were found to be homozygous for the V1016I mutation in the voltage gated sodium channel gene, which is implicated to be involved in insecticide resistance. This strain originating from Clovis, California was resistant to some pyrethroids but not to deltamethrin in bottle bio-assays. Sentinel cage ultra-low-volume (ULV) trials using a new formulation of deltamethrin (DeltaGard®) demonstrated that it provided some control (average of 56% death in sentinel cages in a 91.4 m spray swath) after a single truck mounted aerial ULV application in residential areas.

scholarly journals Non-volant mammal inventory of Cusuco National Park, north-west Honduras: reporting the presence of Jaguar, Panthera onca (Linnaeus, 1758), and demonstrating the effects of zonal protection on mammalian abundance

Check List ◽  
2018 ◽  
Vol 14 (5) ◽  
pp. 877-891
Author(s):  
Hannah M. J. Hoskins ◽  
Oliver J. Burdekin ◽  
Kara Dicks ◽  
Kathy Y. Slater ◽  
Niall P. McCann ◽  
...  
Keyword(s):  
National Park ◽  
Buffer Zone ◽  
Central American ◽  
Panthera Onca ◽  
Mammal Species ◽  
Protection Measures ◽  
North West ◽  
Live Trapping ◽  
A Site ◽  
The Impact

Neotropical cloud forests are one of the most biodiverse yet threatened ecosystems with deforestation and hunting creating major problems. We report a non-volant mammal species inventory for Cusuco National Park, Honduras, spanning 2006–2016. Data were collected using 4 methods: tracks and signs, camera trapping, direct sightings and live trapping. Recorded species of conservation importance included Central American red brocket (Mazama temama (Kerr, 1792)), Baird’s tapir (Tapirus bairdii (Gill, 1865)), margay (Leopardus wiedii (Schinz, 1821)), and jaguar (Panthera onca (Linnaeus, 1758)); the latter recorded for the first time. Cusuco National Park is a site of strategic geographical importance within the Central American jaguar corridor. In total, 43 species were recorded from 26 families. We demonstrate the impact of zonal protection measures with higher mammal abundance recorded in the park’s highly protected core compared to the less protected buffer zone. We advise continued monitoring to provide robust time-series of population trends.

scholarly journals Surveillance, insecticide resistance and control of an invasive Aedes aegypti (Diptera: Culicidae) population in California

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 194 ◽  
Author(s):  
Anthony J. Cornel ◽  
Jodi Holeman ◽  
Catelyn C. Nieman ◽  
Yoosook Lee ◽  
Charles Smith ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Mosquito Species ◽  
Residential Areas ◽  
Mosquito Abundance ◽  
New Locations ◽  
New Formulation ◽  
A Site ◽  
Bg Sentinel ◽  
And Control

The invasion and subsequent establishment in California of Aedes aegypti in 2013 has created new challenges for local mosquito abatement and vector control districts. Studies were undertaken to identify effective and economical strategies to monitor the abundance and spread of this mosquito species as well as for its control. Overall, BG Sentinel (BGS) traps were found to be the most sensitive trap type to measure abundance and spread into new locations. Autocidal-Gravid-Ovitraps (AGO-B), when placed at a site for a week, performed equally to BGS in detecting the presence of female Ae. aegypti. Considering operational cost and our findings, we recommend use of BGS traps for surveillance in response to service requests especially in locations outside the known infestation area. We recommend AGO-Bs be placed at fixed sites, cleared and processed once a week to monitor mosquito abundance within a known infestation area. Long-term high density placements of AGO-Bs were found to show promise as an environmentally friendly trap-kill control strategy. California Ae. aegypti were found to be homozygous for the V1016I mutation in the voltage gated sodium channel gene, which is implicated to be involved in insecticide resistance. This strain originating from Clovis, California was resistant to some pyrethroids but not to deltamethrin in bottle bio-assays. Sentinel cage ultra-low-volume (ULV) trials using a new formulation of deltamethrin (DeltaGard®) demonstrated that it provided some control (average of 56% death in sentinel cages in a 91.4 m spray swath) after a single truck mounted aerial ULV application in residential areas.

scholarly journals Staging Climate Security: Resilience and Heterodystopia in the Bangladesh Borderlands

2018 ◽  
Vol 33 (2) ◽  
pp. 266-294 ◽  
Author(s):  
Jason Cons
Keyword(s):  
Climate Change ◽  
Visual Language ◽  
Ecological Change ◽  
Ground Zero ◽  
The Future ◽  
Development Technology ◽  
To Come ◽  
Warming World ◽  
A Site ◽  
Climate Crisis

This essay interrogates an emergent genre of development projects that seek to instill resilience in populations likely to be severely impacted by climate change. These new projects venture a dark vision of life in a warming world—one where portable technologies become necessary for managing a future of climate chaos. I propose, following Michel Foucault, understanding these projects as heterodystopias: spaces managed as and in anticipation of a world of dystopian climate crisis that are at once stages for future interventions and present-day spectacles of climate security. My exploration of these projects is situated in the borderlands of Bangladesh, a space increasingly imagined as a ground zero of climate change. The projects discussed frame the borderlands as a site that reflects forward onto a multiplicity of (other) dystopian spaces to come. Their often puzzling architecture reveals a grim imagining of the future: one in which atomized resilient families remain rooted in place, facing climate chaos alone, assisted by development technology. In this way, these projects seek to mitigate against global anxiety about climate displacement by emplacing people—preventing them from migrating across borders increasingly imagined as the front lines of climate security. Yet at the same time, these projects speak a visual language that suggests they are as much about representing success at managing climate crisis to an audience elsewhere as they are to successfully stemming climate migration in a particular place. Heterodystopia provides an analytic for diagnosing the specific visions of time and space embedded in securitized framings of the future. In doing so, however, it also points toward counterimaginations and possibilities for life in the midst of ecological change. I thus conclude by contrasting climate heterodystopias with other projects that Bangladeshi peasants living in the borderlands are carrying out: projects that offer different ways of imagining the environment and life in the borderlands of Bangladesh.

Eels Under Standing Wave Conditions

1982 ◽  
Vol 40 ◽  
pp. 492-495
Author(s):  
O.L. Krivanek ◽  
J. TaftØ
Keyword(s):  
Standing Wave ◽  
Wave Conditions ◽  
Set Up ◽  
A Site ◽  
Complex Crystal

It is well known that a standing electron wavefield can be set up in a crystal such that its intensity peaks at the atomic sites or between the sites or in the case of more complex crystal, at one or another type of a site. The effect is usually referred to as channelling but this term is not entirely appropriate; by analogy with the more established particle channelling, electrons would have to be described as channelling either through the channels or through the channel walls, depending on the diffraction conditions.

Head Size and DNA Content in Bacteriophage T4

1972 ◽  
Vol 30 ◽  
pp. 200-201
Author(s):  
Fred Eiserling ◽  
A. H. Doermann ◽  
Linde Boehner
Keyword(s):  
Electron Microscopy ◽  
Dna Content ◽  
Bacteriophage T4 ◽  
Head Size ◽  
Virus Particles ◽  
Altered Protein ◽  
A Cell ◽  
Bacterial Viruses ◽  
A Site ◽  
Protein Shell

The control of form or shape inheritance can be approached by studying the morphogenesis of bacterial viruses. Shape variants of bacteriophage T4 with altered protein shell (capsid) size and nucleic acid (DNA) content have been found by electron microscopy, and a mutant (E920g in gene 66) controlling head size has been described. This mutant produces short-headed particles which contain 2/3 the normal DNA content and which are non-viable when only one particle infects a cell (Fig. 1).We report here the isolation of a new mutant (191c) which also appears to be in gene 66 but at a site distinct from E920g. The most striking phenotype of the mutant is the production of about 10% of the phage yield as “giant” virus particles, from 3 to 8 times longer than normal phage (Fig. 2).

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