Aptamer: A New Class of Oligonucleotide for Therapeutic and
Diagnostic Use - Review

Recently, aptamers get the attention of many scientist, because they have all advantages of antibodies which found in human body. They also have unique merits like thermal stability, low cost, and many more. Aptamers are the short sequence nucleic acid with a high affinity and specificity. It is globally adopted as a one of the advance and promising drug delivery technique. These aptamers are produced by in vitro selection method using SELEX technique. This review contains the discussion on the aspect of design, unique properties, applications of aptamers to aid in cancer diagnosis, prevention and treatment under fine condition. Finally, several medical and analytical applications are presented.

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Aptamers and Possible Effects on Neurodegeneration

Neuroprotection - New Approaches and Prospects ◽

10.5772/intechopen.89621 ◽

2020 ◽

Author(s):

Fatma Söylemez ◽

Çağatay Han Türkseven

Keyword(s):

Thermal Stability ◽

Medical Equipment ◽

Low Cost ◽

Tissue Samples ◽

New Class ◽

High Affinity ◽

The Core ◽

Core Components ◽

Near Future

Aptamers are a new class of recognizing agents which are defined as short biomolecules like oligonucleotides and peptides that are used in diagnostics and therapeutics. They can bind to specific targets with extremely high affinity based on their structural conformations. It is believed that in the near future, aptamers could replace monoclonal antibody. The biggest advantage of using aptamers is that the process is in vitro in nature and does not require the use of animals and they also have unique properties, such as thermal stability, low cost, and unlimited applications. Aptamers have been studied as a biomaterial in numerous investigations concerning their use as a diagnostic and therapeutic tool and biosensing probe. DNA aptamers were also used for the diagnosis and treatment of neurodegeneration and neurodegenerative diseases. For example, functional nucleic acid aptamers have been developed to detect Aβ fragments in Alzheimer’s brain hippocampus tissue samples. Aptamers are promising materials for diverse areas, not just as alternatives to antibodies but as the core components of medical equipment. Although they are in the preliminary stages of development, results are quite encouraging, and it seems that aptamer research has a very bright future in neuroscience.

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In vitro selection of high affinity anti-retinal antigens (S-antigen, IRBP, rhodopsin) antibodies from a combinatorial phage display library derived from a patient with autoimmune uveitis

Immunology Letters ◽

10.1016/s0165-2478(97)86521-2 ◽

1997 ◽

Vol 56 ◽

pp. 377

Author(s):

S. O'Brien ◽

J. Liversidge ◽

J.V. Forrester ◽

W.J. Harris

Keyword(s):

Phage Display ◽

In Vitro Selection ◽

Phage Display Library ◽

Autoimmune Uveitis ◽

High Affinity ◽

Retinal Antigens ◽

Selection Of

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Ribosome Display Technology: Applications in Disease Diagnosis and Control

Antibodies ◽

10.3390/antib9030028 ◽

2020 ◽

Vol 9 (3) ◽

pp. 28

Author(s):

Adinarayana Kunamneni ◽

Christian Ogaugwu ◽

Steven Bradfute ◽

Ravi Durvasula

Keyword(s):

In Vitro Selection ◽

Low Cost ◽

Disease Diagnosis ◽

Single Chain ◽

Single Chain Antibody ◽

Ribosome Display ◽

Technology Applications ◽

Display Technology ◽

And Control

Antibody ribosome display remains one of the most successful in vitro selection technologies for antibodies fifteen years after it was developed. The unique possibility of direct generation of whole proteins, particularly single-chain antibody fragments (scFvs), has facilitated the establishment of this technology as one of the foremost antibody production methods. Ribosome display has become a vital tool for efficient and low-cost production of antibodies for diagnostics due to its advantageous ability to screen large libraries and generate binders of high affinity. The remarkable flexibility of this method enables its applicability to various platforms. This review focuses on the applications of ribosome display technology in biomedical and agricultural fields in the generation of recombinant scFvs for disease diagnostics and control.

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