scholarly journals DNA Barcoding of Selected Medicinal Plant Species from Jordan Using matK, rbcL, and rpoC1 Genes

Author(s):  
Almuthanna K. Alkaraki ◽  
Maisam A. Aldmoor ◽  
Jamil N. Lahham ◽  
Shreen D. Nusair

Medicinal plants play an essential role in preventing and treating several diseases. Classical taxonomic tools generally carry out medicinal plant identification and characterization. Unfortunately, conventional methods need well-trained taxonomists and could give a false identity for closely related species. Jordanian flora is rich in a variety of plant species. The phylogeographic architecture of Jordanian medicinal plant samples was not explored yet. This study aims to recruit DNA barcoding using matK, rbcL, and rpoC1 genes to identify different selected medicinal plants species from Jordan. These are Maerua crassifolia, Ziziphus spina-christi, Balanites aegyptiaca, Senna italica, and Moringa peregrina. Plant samples were collected from the Dead Sea area (Jordan), and three DNA barcode regions were amplified, sequenced, and analyzed using different bioinformatic tools. Twelve sequences were obtained and deposited in Genbank . These sequences showed a very good discrimination capacity with sequences retrieved from related species. The phylogenetic analysis illustrated that DNA barcoding could successfully identify the selected medicinal plant species using different chloroplast genes (rbcL, matK, and rpoC1). Further analysis for other plants species is recommended to explore the genetic relationship and the phylogeographic architecture for Jordanian flora.

2021 ◽  
Vol 2021 ◽  
pp. 1-25
Author(s):  
Sidra Ahsan Shah ◽  
Wajeeha Iqbal ◽  
Muneeba Sheraz ◽  
Bilal Javed ◽  
Syeda Sadaf Zehra ◽  
...  

Bajwat Wildlife Sanctuary is a complex riverine ecosystem and is unique because of the presence of river Chenab, various seasonal streams, lakes, and Head Marala barrage. These ecogeographic conditions provide diverse natural habitats for various plant and animal species to grow uninterrupted and have undocumented ethnopharmacologically important medicinal flora. The present study involves the first-ever extensive investigation to document the ethnopharmacological knowledge on medicinal plants of local healers and inhabitants of the Bajwat Wildlife Sanctuary to treat ailments. The unstructured and semistructured interviews of the local healers and inhabitants were conducted that included 130 individuals. The ethnomedicinal formulations, their method of preparation, mode of administration, parts of the plant used, diseases cured, and their categorization along with species use report (UR) were analyzed. The ethnopharmacological study led to the enlisting of 114 medicinal plant species belonging to 97 genera and distributed among 47 plant families. 2029 URs were collected with 42 general disease categories. Each plant species was reported 18 times to cure various diseases (∼18 UR), while ∼48 URs were collected on each disease category by local informants. Digestive issues (290 URs, ∼14.29%) and skin infections (279 URs, ∼13.75%) were found most commonly among the occupants of the area. The oral administration (69%) of herbal drugs and the preparation of plant extracts (32%) were the most common ethnopharmacological strategies. Inhabitants of the area were well aware of the limited use of poisonous plants. 8 (∼7%) out of the total 114 medicinal plant species were listed in the IUCN Red List of Threatened Species as Least Concern, while Eucalyptus camaldulensis Dehnh. was enlisted as near-threatened. The results of the present investigation show that the occupants of the Bajwat have sound information about the ethnopharmacological consumption of medicinal plants, and some of the novel ethnomedicinal formulations were reported which provide the basic data for further pharmacological research.


PLoS ONE ◽  
2010 ◽  
Vol 5 (1) ◽  
pp. e8613 ◽  
Author(s):  
Shilin Chen ◽  
Hui Yao ◽  
Jianping Han ◽  
Chang Liu ◽  
Jingyuan Song ◽  
...  

Author(s):  
S.Zh. Erekeyeva ◽  
◽  
R.B. Arysbayeva ◽  
A. Musrat ◽  
László Orlóci ◽  
...  

Rosaceae Juss. family have more cosmopolitan plants growing in many parts of the globe. Apart from food and decorative plants, there are also medicinal plant species. Increasing the potential of the use of medicinal plants currently represents one of the top-priority objectives that requires a comprehensive study of plants in isolation from natural flora, growing and preserving the species. The article focuses on Kazakhstani plant species of the Rosaceae Juss. family, and specifics of their growth and distribution. Endangered species, as reported in the literature and online sources, are briefly described. Also, the specifics of introducing 30 plant species of family Rosaceae Juss. from global and Kazakhstan flora in the Main Botanic Garden of Almaty are described, introduction parameters (phenological observation and seed quality parameters) are analyzed for 7 medicinal plant species (Agrimonia asiatica Juz., Filipendula ulmaria (L.) Maxim., Filipendula vulgaris Moench, Potentilla argentea L., Potentilla recta L., Fragaria vesca L., and Fragaria viridis (Duch.) Weston) are introduced from the Northern Tien Shan flora, and a brief summary of the introduction details obtained on the basis of the successful introduction index is provided.


2018 ◽  
Vol 1 (2) ◽  
pp. 58-64
Author(s):  
Dwi Haryanto ◽  
Rosye H.R. Tanjung ◽  
Konstantina M.B. Kameubun

Study on the used of medicinal plants by Marind people who lived at Wasur National Park, Merauke was conducted by using descriptive methods which include observation, interview, documentation, literature review techniques, as well as  emic and ethic approaches. During the study there were 46 species which belong to 26 families plants found as medicinal plants used by Marind people to cure about 30 diseases. Among 46 species, there were 7 potential medicinal plant species which known  and used by most Marind people. The seven potential medicinal plant species were Ipomoea triloba L., Bauhinia sp., Pittosporum sp., Kingiodendron platycarpum Bent., Sophora tool mentosa L.Cyrtandra sp., dan Tinopspora disstiflora L. Part of plant used vary from leaf, root, bark, fruit and other part of plant. Compare to other part of plant, leaf was the most common used as traditional medicinal plant. Key words:   traditional medicinal plant, Marind people, Wasur National Park


2021 ◽  
pp. 1-13
Author(s):  
Sangay Tenzin ◽  
Pema Tendar

This paper documents the medicinal plant species in wetlands of the Eastern Himalayan Highlands of Gasa District, Bhutan. A random quadrat sampling method was used to assess the presence-absence of species. The study was conducted at Khatoed and Khamaed gewogs (blocks), Gasa District between January and December 2018. Identification of medicinal plant species and families were done in consultation with a botanist at the herbarium in Thimphu and volumes of Flora of Bhutan were referred. Based on the published and authentic scientific literature, information on medical uses of plant parts was collected. A total of 74 species of medicinal plant species in the wetlands, distributed in 69 genera and 43 families have been recorded. The study suggests preserving invaluable wetland medicinal plants and integrating ethno-medicinal practices in our lives.


2021 ◽  
Author(s):  
Boja Tilinti Bose ◽  
Yoseph Melka ◽  
Tesfaye Awas

Abstract Background: The world population use medicinal plants for treating diseases in forms of drug developments also traditional medicinal specially developing countries. The economic growth and cultural changes threaten the traditional lifestyle of local people. The aim of the study is to investigate medicinal plants and associated indigenous knowledge. Methods: Ethnobotanical data were obtained using semi-structured interviews, focus group discussion and market survey. Data were analyzed by descriptive statistics, informant consensus factor, preference ranking and Direct matrix ranking using Microsoft Excel and Statistical Package for Social Science Version 20. Results: Ethnomedicinal use of 136 medicinal plant species belonging to 117 genera and 63 families wereidentified and documented. The highest number of medicinal plants was used in midland kebele(74 species). Out of total medicinal plants 66 (48.53%) species were reported to treat human aliments only. Most of the medicinal plants harvested were herbs (37.50%), followed by trees (36.76%) and the least was lianas (0.74%). A Pearson correlation test indicated a positive and significant difference between age of informants and number of medicinal plant species cited, and negative and significant difference between level of education and number of medicinal plant species cited. The result indicates that there was significant difference between male and female informants in their knowledge of medicinal plants, and there was significant different between number of medicinal plants reported by key informants and household respondents. Cordia africana ranked first as a multipurpose medicinal plant. Agricultural expansion (41.96%) stood first as a factor threatening medicinal plants. Conclusion: The highest numbers of MPs were used in Sire Morose kebele (midland), but collection was not restricted only to their locality the move other kebeles to harvest MPs. In the study area elders have better knowledge of medicinal plant than younger and as people educate their knowledge on medicinal plants decrease.This implies that in study area there is potential indigenous knowledge but we are going loss this traditional knowledge if did not develop means transferring this to next generation. To support local people effort on in-situ conservation and ex-situ conservation needed


2013 ◽  
Vol 1 (04) ◽  
pp. 13-15 ◽  
Author(s):  
B. B.S. Kapoor ◽  
Veena Purohit

Evaluation of sterol contents from three selected medicinal plant species of Fabaceae family growing in Rajasthan Desert was carried out. The roots, shoots and fruits of Clitoria ternatea, Sesbania bispinosa and Tephrosia purpurea were analysed for sterol contents. - Sitosterol and Stigmasterol were isolated and identified. Maximum sterol contents were observed in shoots of Sesbania bispinosa (0.29 mg/g.d.w.), whereas minimum in roots of Tephrosia purpurea(0.15mg/g.d.w.


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