scholarly journals Interaction of cationic antiseptics with cardiolipin-containing model bacterial membranes

2021 ◽  
Author(s):  
EG Kholina ◽  
ME Bozdaganyan ◽  
MG Strakhovskaya ◽  
IB Kovalenko
Keyword(s):  
Lipid Bilayer ◽  
Plasma Membranes ◽  
Lateral Diffusion ◽  
Coarse Grained ◽  
Membrane Area ◽  
Bacterial Membranes ◽  
Fatty Acid Chain ◽  
Lipid Ratio ◽  
Lipid Fatty Acid ◽  
Area Per Lipid

Plasma membrane is one of the major targets for cationic antiseptics (CA). The study was aimed to assess molecular effects of CAs of different chemical classes on cardiolipin-containing regions of bacterial plasma membranes. The study was carried out using coarse-grained molecular modeling. Interaction of CAs, such as miramistin, chlorhexidine, picloxidine, and octenidine, with cardiolipin-containing bilayer was assessed based on the CA coarse-grained models. CAs reduced lipid lateral diffusion coefficients and increased the membrane area per lipid. All CAs, except miramistin, reduced the lipid fatty acid chain order parameters. Adding octenidine at a CA : lipid ratio of 1 : 4 resulted in cardiolipin clustering with subsequent pulling the neutral phosphatidylethanolamine molecules out of the model bilayer. It was found that CАs have the potential for sorption to lipid bilayer, causing clustering of negatively charged lipids. Antiseptic octenidine causes formation of cardiolipin microdomains. Abnormal lateral lipid distribution together with pulling out phosphatidylethanolamine molecules can result in increased lipid bilayer permeability. The most significant reduction of cardiolipin lateral diffusion coefficient by 2.8 ± 0.4 times was observed in the presence of CA chlorhexidine at an antiseptic : lipid ratio of 1 : 4.

Lipopeptides as anti-infectives: a practical perspective

2009 ◽  
Vol 4 (3) ◽  
pp. 258-273 ◽  
Author(s):  
Giovanna Pirri ◽  
Andrea Giuliani ◽  
Silvia Nicoletto ◽  
Lorena Pizzuto ◽  
Andrea Rinaldi
Keyword(s):  
Fatty Acid ◽  
Lipid Bilayer ◽  
Cyclic Peptide ◽  
Chemical Modifications ◽  
Bacterial Membranes ◽  
Therapeutic Applications ◽  
Fatty Acid Chain ◽  
Lipopeptide Antibiotics

AbstractLipopeptide antibiotics represent an old class of antibiotics that were discovered over 50 years ago, which includes the old polymyxins but also new entries, such as the recently approved daptomycin. They generally consist of a hydrophilic cyclic peptide portion attached to a fatty acid chain which facilitates insertion into the lipid bilayer of bacterial membranes. This review presents an overview of this class of antibiotics, focusing on their therapeutic applications and putting particular emphasis on chemical modifications introduced to improve their activity.

scholarly journals A quantitative model of traffic between plasma membrane and secondary lysosomes: evaluation of inflow, lateral diffusion, and degradation.

1988 ◽  
Vol 107 (6) ◽  
pp. 2109-2115 ◽  
Author(s):  
J P Draye ◽  
P J Courtoy ◽  
J Quintart ◽  
P Baudhuin
Keyword(s):  
Plasma Membrane ◽  
Half Life ◽  
Plasma Membranes ◽  
Lateral Diffusion ◽  
Quantitative Model ◽  
Lysosomal Membrane ◽  
Membrane Area ◽  
Rat Fibroblasts ◽  
Secondary Lysosomes ◽  
Total Membrane

We present here a mathematical model that accounts for the various proportions of plasma membrane constituents occurring in the lysosomal membrane of rat fibroblasts (Draye, J.-P., J. Quintart, P. J. Courtoy, and P. Baudhuin. 1987. Eur. J. Biochem. 170: 395-403; Draye, J.-P., P. J. Courtoy, J. Quintart, and P. Baudhuin. 1987. Eur. J. Biochem. 170:405-411). It is based on contents of plasma membrane markers in purified lysosomal preparations, evaluations of their half-life in lysosomes and measurements of areas of lysosomal and plasma membranes by morphometry. In rat fibroblasts, structures labeled by a 2-h uptake of horseradish peroxidase followed by a 16-h chase (i.e., lysosomes) occupy 3% of the cellular volume and their total membrane area corresponds to 30% of the pericellular membrane area. Based on the latter values, the model predicts the rate of inflow and outflow of plasma membrane constituents into lysosomal membrane, provided their rate of degradation is known. Of the bulk of polypeptides iodinated at the cell surface, only 4% reach the lysosomes every hour, where the major part (integral of 83%) is degraded with a half-life in lysosomes of integral to 0.8 h. For specific plasma membrane constituents, this model can further account for differences in the association to the lysosomal membrane by variations in the rate either of lysosomal degradation, of inflow along the pathway from the pericellular membrane to the lysosomes, or of lateral diffusion.

scholarly journals A Coarse-Grained Molecular Dynamics Study of DLPC, DMPC, DPPC, and DSPC Mixtures in Aqueous Solution

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Roghayeh Abedi Karjiban ◽  
Nurul Syahidah Shaari ◽  
Uma Villashini Gunasakaran ◽  
Mahiran Basri
Keyword(s):  
Molecular Dynamics ◽  
Aqueous Solution ◽  
Md Simulation ◽  
Mixed Model ◽  
Lateral Diffusion ◽  
Coarse Grained ◽  
Isotropic Pressure ◽  
Area Per Lipid ◽  
Mixture System ◽  
Coarse Grained Molecular Dynamics

The structural and dynamics properties of the bilayer comprising 128 molecules of dipalmitoylphosphatidylcholine (DPPC), dilauroylphosphatidylcholine (DLPC), dimyristoylphosphatidylcholine (DMPC), and distearoylphosphatidylcholine (DSPC) in water were investigated using a coarse-grained molecular dynamics (CG-MD) simulation technique. The model mixture system was simulated at 298 K under semi-isotropic pressure conditions. The aggregation was initiated from the random configurations followed by the formation of a bilayer over a period of 500 ns. The calculated values of the area per lipid, thickness, and lateral diffusion for the mixed model were different from when a single lipid was used. Our results confirmed that the chain length of the lipid molecules strongly affects the phospholipid bilayer’s physical properties.

scholarly journals LION/web: a web-based ontology enrichment tool for lipidomic data analysis

GigaScience ◽  
2019 ◽  
Vol 8 (6) ◽  
Author(s):  
Martijn R Molenaar ◽  
Aike Jeucken ◽  
Tsjerk A Wassenaar ◽  
Chris H A van de Lest ◽  
Jos F Brouwers ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Plasma Membranes ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Lateral Diffusion ◽  
Decanoic Acid ◽  
Web Based ◽  
Ovary Cells ◽  
Lipid Species ◽  
Significant Enrichment

Abstract Background A major challenge for lipidomic analyses is the handling of the large amounts of data and the translation of results to interpret the involvement of lipids in biological systems. Results We built a new lipid ontology (LION) that associates >50,000 lipid species to biophysical, chemical, and cell biological features. By making use of enrichment algorithms, we used LION to develop a web-based interface (LION/web, www.lipidontology.com) that allows identification of lipid-associated terms in lipidomes. LION/web was validated by analyzing a lipidomic dataset derived from well-characterized sub-cellular fractions of RAW 264.7 macrophages. Comparison of isolated plasma membranes with the microsomal fraction showed a significant enrichment of relevant LION-terms including “plasma membrane", “headgroup with negative charge", "glycerophosphoserines", “above average bilayer thickness", and “below average lateral diffusion". A second validation was performed by analyzing the membrane fluidity of Chinese hamster ovary cells incubated with arachidonic acid. An increase in membrane fluidity was observed both experimentally by using pyrene decanoic acid and by using LION/web, showing significant enrichment of terms associated with high membrane fluidity ("above average", "very high", and "high lateral diffusion" and "below average transition temperature"). Conclusions The results demonstrate the functionality of LION/web, which is freely accessible in a platform-independent way.

scholarly journals Simian Virus 40 Late Proteins Possess Lytic Properties That Render Them Capable of Permeabilizing Cellular Membranes

2006 ◽  
Vol 80 (13) ◽  
pp. 6575-6587 ◽  
Author(s):  
Robert Daniels ◽  
Nasser M. Rusan ◽  
Anne-Kathrin Wilbuer ◽  
Leonard C. Norkin ◽  
Patricia Wadsworth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasma Membranes ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Cellular Membranes ◽  
Bacterial Membranes ◽  
Cell Death Pathways ◽  
Late Protein ◽  
Temporally Correlated ◽  
Infectious Cycle

ABSTRACT Many nonenveloped viruses have evolved an infectious cycle that culminates in the lysis or permeabilization of the host to enable viral release. How these viruses initiate the lytic event is largely unknown. Here, we demonstrated that the simian virus 40 progeny accumulated at the nuclear envelope prior to the permeabilization of the nuclear, endoplasmic reticulum, and plasma membranes at a time which corresponded with the release of the progeny. The permeabilization of these cellular membranes temporally correlated with late protein expression and was not observed upon the inhibition of their synthesis. To address whether one or more of the late proteins possessed an inherent capacity to induce membrane permeabilization, we examined the permeability of Escherichia coli that separately expressed the late proteins. VP2 and VP3, but not VP1, caused the permeabilization of bacterial membranes. Additionally, VP3 expression resulted in bacterial cell lysis. These findings demonstrate that VP3 possesses an inherent lytic property that is independent of eukaryotic signaling or cell death pathways.

Extension of the UNRES Coarse-Grained Force Field to Membrane Proteins in the Lipid Bilayer

2019 ◽  
Vol 123 (37) ◽  
pp. 7829-7839
Author(s):  
Karolina Ziȩba ◽  
Magdalena Ślusarz ◽  
Rafał Ślusarz ◽  
Adam Liwo ◽  
Cezary Czaplewski ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Force Field ◽  
Lipid Bilayer ◽  
Coarse Grained

scholarly journals Tracking Lysosome Migration within Chinese Hamster Ovary (CHO) Cells Following Exposure to Nanosecond Pulsed Electric Fields

2018 ◽  
Vol 5 (4) ◽  
pp. 103
Author(s):  
Gary Thompson ◽  
Hope Beier ◽  
Bennett Ibey
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Mammalian Cells ◽  
Plasma Membranes ◽  
Chinese Hamster ◽  
Lateral Diffusion ◽  
Cell Swelling ◽  
Dependent Manner ◽  
Membrane Repair ◽  
Osmotic Swelling

Above a threshold electric field strength, 600 ns-duration pulsed electric field (nsPEF) exposure substantially porates and permeabilizes cellular plasma membranes in aqueous solution to many small ions. Repetitive exposures increase permeabilization to calcium ions (Ca2+) in a dosage-dependent manner. Such exposure conditions can create relatively long-lived pores that reseal after passive lateral diffusion of lipids should have closed the pores. One explanation for eventual pore resealing is active membrane repair, and an ubiquitous repair mechanism in mammalian cells is lysosome exocytosis. A previous study shows that intracellular lysosome movement halts upon a 16.2 kV/cm, 600-ns PEF exposure of a single train of 20 pulses at 5 Hz. In that study, lysosome stagnation qualitatively correlates with the presence of Ca2+ in the extracellular solution and with microtubule collapse. The present study tests the hypothesis that limitation of nsPEF-induced Ca2+ influx and colloid osmotic cell swelling permits unabated lysosome translocation in exposed cells. The results indicate that the efforts used herein to preclude Ca2+ influx and colloid osmotic swelling following nsPEF exposure did not prevent attenuation of lysosome translocation. Intracellular lysosome movement is inhibited by nsPEF exposure(s) in the presence of PEG 300-containing solution or by 20 pulses of nsPEF in the presence of extracellular calcium. The only cases with no significant decreases in lysosome movement are the sham and exposure to a single nsPEF in Ca2+-free solution.

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