scholarly journals Antibiotic and Heavy Metal Resistance of Bacteria Isolated from Diseased Mud Crab (Scylla serrata)

2015 ◽  
Vol 3 (3) ◽  
pp. 1-15
Author(s):  
Cik Syahrizawati M Z. ◽  
Ikhwanuddin M. ◽  
Wendy W. ◽  
Zulhisyam AK ◽  
Lee SW
Keyword(s):  
Heavy Metal ◽  
Vibrio Parahaemolyticus ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Edwardsiella Tarda ◽  
Scylla Serrata ◽  
Bacterial Isolates ◽  
Mud Crab ◽  
Salmonella Spp ◽  
Klebsiella Spp

A total of 320 bacteria isolated from marketable size and diseased mud crab (Scylla serrata) at a commercial farm. The isolated bacteria were Aeromonas spp. n = 70, Edwardsiella tarda n = 50, Vibrio alginolyticus n = 40, Vibrio parahaemolyticus n = 20, Salmonella spp. n = 70 and Klebsiella spp. n = 70. All the bacterial isolates were tested for antibiotic susceptibility against 16 types of antibiotics by using disk diffusion method. The antibiotics tested in this study were nalidixic acid (30 ?g/disk), oxolinic acid (2 ?g/disk), compound sulphonamides (300 ?g/disk), doxycycline (30 ?g/disk), tetracycline (30 ?g/disk), novobiocin (30 ?g/disk), chloramphenicol (30 ?g/disk), kanamycin (30 ?g/disk), sulphamethoxazole (25 ?g/disk), flumequine (30 ?g/disk), erythromycin (15 ?g/disk), ampicillin (10 ?g/disk), spiramycin (100 ?g/disk), oxytetracycline (30 ?g/disk), amoxycillin (25 ?g/disk) and fosfomycin (50 ?g/disk). Heavy metal resistance pattern of the present bacterial isolates was also characterized against mercury (Hg2+), chromium (Cr6+), copper (Cu2+), and Zinc (Zn2+) by using two fold agar dilution method. The percentage of antibiotic sensitivity of the present bacterial isolates was ranged from 12.5 % to 100 % in which most of the present bacteria isolates were not sensitive to ampicillin whereas all the bacteria isolates were sensitive to nalidixic acid, flumequine and oxytetracycline. Overall, the total of antibiotic sensitive case was reported as 72.7% whereas antibiotic resistance and intermediate sensitive case was recorded as 19.7% and 7.4 %, respectively. The Multiple Antibiotic Resistance (MAR) values were range of 0.03 to 0.29 in which Aeromonas spp (0.29) showed the highest value of MAR. This was followed by Salmonella spp. (0.21) and Klebsiella spp. (0.21), Edwardsiella tarda (0.20), Vibrio alginolyticus (0.09) and Vibrio parahaemolyticus (0.03). The MAR value indicated that the commercial S. serrata were not contaminated to the test antibiotics. Furthermore, low resistance activity of the present bacterial isolates to the tested heavy metals (Cr6+: 20.7 % to 30.8 %, Zn2+: 0 % to 40 %, Cu2+: 18.8 % to 25 % and Hg2+: 30 % to 33.3 %) was observed.

scholarly journals Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from fresh shrimps in Shanghai fish markets, China

2016 ◽  
Vol 23 (15) ◽  
pp. 15033-15040 ◽  
Author(s):  
Yu He ◽  
Lanlan Jin ◽  
Fengjiao Sun ◽  
Qiongxia Hu ◽  
Lanming Chen
Keyword(s):  
Heavy Metal ◽  
Vibrio Parahaemolyticus ◽  
Heavy Metal Resistance ◽  
Metal Resistance

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

Occurrence of Virulence Factors and Antibiotic and Heavy Metal Resistance in Vibrio parahaemolyticus Isolated from Pacific Mackerel at Markets in Zhejiang, China

2020 ◽  
Vol 83 (8) ◽  
pp. 1411-1419
Author(s):  
JIEHONG FANG ◽  
HUI CHENG ◽  
TING YU ◽  
HAN JIANG
Keyword(s):  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Virulence Factors ◽  
Vibrio Parahaemolyticus ◽  
Resistance Index ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Resistance Testing ◽  
Multiple Antibiotic Resistance ◽  
Pacific Mackerel

ABSTRACT Vibrio parahaemolyticus is a widespread bacterium in the marine environment and is one of the leading causes of food-derived bacterial poisoning in humans worldwide. The main objective of this study was to determine the prevalence, virulence factors, and antibiotic and heavy metal resistance profiles of V. parahaemolyticus in Pacific mackerel (Pneumatophorus japonicus) from different markets in Zhejiang Province, People's Republic of China. In total, 112 (31.11%) V. parahaemolyticus isolates were identified from 360 Pacific mackerel samples, with an extremely low occurrence of the virulence genes trh (1.79%, 2 of 112) and tdh (0%, 0 of 112). Antibiotic resistance testing revealed that most isolates showed resistance to ampicillin (96.43%, 108 of 112) and streptomycin (90.18%, 101 of 112), whereas all strains were sensitive to kanamycin, florfenicol, cefamandole, and trimethoprim-sulfamethoxazole. Furthermore, 46.43% (52 of 112) of isolates, which had 12 different phenotypes, were classified as multidrug resistant. In addition, the multiple antibiotic resistance index values of isolates were between 0.05 and 0.63, and the maximum multiple antibiotic resistance index was attributed to two isolates that exhibited resistance to 12 antibiotics. Heavy metal resistance patterns were similar among the six different markets. The majority of isolates showed resistance to Cd2+ (78.57%) and Pb2+ (51.79%), and fewer were resistant to Cu2+ (37.50%), Zn2+ (25.00%), Co2+ (9.82%), Ni2+ (6.25%), and Mn2+ (4.46%). No isolates were resistant to Cr3+. In total, 22.32% (25 of 112) of strains were multiheavy metal resistant. Furthermore, multidrug resistance and multiheavy metal resistance were found to be positively correlated in the V. parahaemolyticus strains by using Pearson's correlation analysis (P = 0.008; R = 0.925). This information will contribute to the monitoring of variations in the antibiotic and heavy metal resistance profiles of V. parahaemolyticus strains from seafood and provide insight into the appropriate use of antibiotics and the safe consumption of seafood. HIGHLIGHTS

scholarly journals Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies

2020 ◽  
Vol 27 (10) ◽  
pp. 2660-2668
Author(s):  
Insha Sultan ◽  
Asghar Ali ◽  
Firdoos A. Gogry ◽  
Irfan A. Rather ◽  
Jamal S.M. Sabir ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Mobile Genetic Elements ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Water Bodies ◽  
Bacterial Isolates ◽  
Genetic Elements

Plasmids associated with heavy metal resistance and herbicide degradation potential in bacterial isolates obtained from two Brazilian regions

2019 ◽  
Vol 191 (5) ◽  
Author(s):  
Jéssica Aparecida Silva Moretto ◽  
Vânia Santos Braz ◽  
João Pedro Rueda Furlan ◽  
Eliana Guedes Stehling
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Bacterial Isolates ◽  
Herbicide Degradation

scholarly journals Identification Of Cu Resistant Bacteria From Tin Mining-Affected Sea Sediment

2020 ◽  
Vol 6 (2) ◽  
pp. 112-119
Author(s):  
Rahmad Lingga ◽  
Budi Afriyansyah
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Heavy Metal Resistance ◽  
Negative Influence ◽  
Metal Resistance ◽  
Mining Activity ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Gram Staining

Tin mining activity at sea has a various negative influence on the environment. One of them is heavy metal contamination that can affect the life of fisheries biota. This research conducted to isolate and test the Cu heavy metal resistance of marine sediment bacteria that are affected by tin mining activity. Sediment sampling was carried out in the area of tin mining to the mudflat neared to the mangrove area. Bacterial isolation was carried out by spread plate method and bacterial characterization included cell shape, Gram staining and biochemical tests. Furthermore, bacterial isolates tested for resistance to metals with concentrations of 10 ppm, 20 ppm, 40 ppm, 80 ppm and 100 ppm. The results showed that bacterial isolates originating from marine sediments affected by mining activity were resistant to Cu heavy metal at various concentrations. Isolates B6, B8 and A10 showed the highest resistance up to a concentration of 100 ppm

scholarly journals Heavy metal resistance properties of bacteria from different soil types in Horo Guduru Wollega, Ethiopia

2019 ◽  
Vol 5 (11) ◽  
pp. 320
Author(s):  
Melkamu T. Addisu ◽  
Adugna M. Bikila
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Agricultural Soils ◽  
Environmental Concern ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Soil Types ◽  
Resistant Bacteria ◽  
Bacterial Isolates

<p class="abstract"><strong>Background:</strong> The quality of life on earth is linked inseparably to the overall quality of the environment. Soil pollution with heavy metals has become a critical environmental concern due to its potential adverse ecological effects. The study explored the heavy metals resistance properties of bacteria isolated from fertilizer applied agricultural and non-agricultural soils.</p><p class="abstract"><strong>Methods:</strong> The soil samples were collected from both fertilizer applied agricultural soils and non-agricultural soils. After identification and characterization of the isolates from both soil types, six (6) similar bacterial isolates were selected to screen for resistance against Cobalt (Co<sup>+</sup>), Lead (Pb<sup>2+</sup>), Cromium (Cr<sup>+3</sup>), Mercury (Hg<sup>2+</sup>), Nickel (Ni<sup>2+</sup>), Cadmium (Cd<sup>2+</sup>) and Zinc (Zn<sup>2+</sup>) heavy metals. The minimum inhibitory concentration (MIC) for the bacterial isolates were determined by gradually increasing the concentration of heavy metals on agar plates until the isolates failed to show growth.  </p><p class="abstract"><strong>Results:</strong> The isolates from fertilizer applied agricultural soil showed the highest resistance against the selected heavy metals than those isolated from fertilizers not applied (nonagricultural) soils.</p><p><strong>Conclusions:</strong> From this result it can be seen that fertilizer has significant role in influencing the heavy metal resistance properties of bacteria and these heavy metal resistant bacteria can be useful for the bioremediation of heavy metal contaminated environment. </p>

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