scholarly journals Identification Of Cu Resistant Bacteria From Tin Mining-Affected Sea Sediment

2020 ◽  
Vol 6 (2) ◽  
pp. 112-119
Author(s):  
Rahmad Lingga ◽  
Budi Afriyansyah
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Contamination ◽  
Heavy Metal Resistance ◽  
Negative Influence ◽  
Metal Resistance ◽  
Mining Activity ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Gram Staining

Tin mining activity at sea has a various negative influence on the environment. One of them is heavy metal contamination that can affect the life of fisheries biota. This research conducted to isolate and test the Cu heavy metal resistance of marine sediment bacteria that are affected by tin mining activity. Sediment sampling was carried out in the area of tin mining to the mudflat neared to the mangrove area. Bacterial isolation was carried out by spread plate method and bacterial characterization included cell shape, Gram staining and biochemical tests. Furthermore, bacterial isolates tested for resistance to metals with concentrations of 10 ppm, 20 ppm, 40 ppm, 80 ppm and 100 ppm. The results showed that bacterial isolates originating from marine sediments affected by mining activity were resistant to Cu heavy metal at various concentrations. Isolates B6, B8 and A10 showed the highest resistance up to a concentration of 100 ppm

scholarly journals Heavy metal resistance properties of bacteria from different soil types in Horo Guduru Wollega, Ethiopia

2019 ◽  
Vol 5 (11) ◽  
pp. 320
Author(s):  
Melkamu T. Addisu ◽  
Adugna M. Bikila
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Agricultural Soils ◽  
Environmental Concern ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Soil Types ◽  
Resistant Bacteria ◽  
Bacterial Isolates

<p class="abstract"><strong>Background:</strong> The quality of life on earth is linked inseparably to the overall quality of the environment. Soil pollution with heavy metals has become a critical environmental concern due to its potential adverse ecological effects. The study explored the heavy metals resistance properties of bacteria isolated from fertilizer applied agricultural and non-agricultural soils.</p><p class="abstract"><strong>Methods:</strong> The soil samples were collected from both fertilizer applied agricultural soils and non-agricultural soils. After identification and characterization of the isolates from both soil types, six (6) similar bacterial isolates were selected to screen for resistance against Cobalt (Co<sup>+</sup>), Lead (Pb<sup>2+</sup>), Cromium (Cr<sup>+3</sup>), Mercury (Hg<sup>2+</sup>), Nickel (Ni<sup>2+</sup>), Cadmium (Cd<sup>2+</sup>) and Zinc (Zn<sup>2+</sup>) heavy metals. The minimum inhibitory concentration (MIC) for the bacterial isolates were determined by gradually increasing the concentration of heavy metals on agar plates until the isolates failed to show growth.  </p><p class="abstract"><strong>Results:</strong> The isolates from fertilizer applied agricultural soil showed the highest resistance against the selected heavy metals than those isolated from fertilizers not applied (nonagricultural) soils.</p><p><strong>Conclusions:</strong> From this result it can be seen that fertilizer has significant role in influencing the heavy metal resistance properties of bacteria and these heavy metal resistant bacteria can be useful for the bioremediation of heavy metal contaminated environment. </p>

scholarly journals Antibiotic and Heavy Metal Resistant Bacteria Isolated from Aegean Sea Water and Sediment in Güllük Bay, Turkey : Quantifying the resistance of identified bacteria species with potential for environmental remediation applications

2020 ◽  
Vol 64 (4) ◽  
pp. 507-525 ◽  
Author(s):  
Gülşen Altuğ ◽  
Mine Çardak ◽  
Pelin Saliha Çiftçi Türetken ◽  
Samet Kalkan ◽  
Sevan Gürün
Keyword(s):  
Heavy Metal ◽  
Aegean Sea ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Heavy Metal Resistant ◽  
Seawater Samples

Heavy metal and antibiotic-resistant bacteria have potential for environmental bioremediation applications. Resistant bacteria were investigated in sediment and seawater samples taken from the Aegean Sea, Turkey, between 2011 and 2013. Bioindicator bacteria in seawater samples were tested using the membrane filtration technique. The spread plate technique and VITEK® 2 Compact 30 micro identification system were used for heterotrophic aerobic bacteria in the samples. The minimum inhibition concentration method was used for heavy metal-resistant bacteria. Antibiotic-resistant bacteria were tested using the disk diffusion method. All bacteria isolated from sediment samples showed 100% resistance to rifampicin, sulfonamide, tetracycline and ampicillin. 98% of isolates were resistant against nitrofurantoin and oxytetracycline. Higher antibiotic and heavy metal resistance was recorded in bacteria isolated from sediment than seawater samples. The highest levels of bacterial metal resistance were recorded against copper (58.3%), zinc (33.8%), lead (32.1%), chromium (31%) and iron (25.2%). The results show that antibiotic and heavy metal resistance in bacteria from sediment and seawater can be observed as responses to environmental influences including pollution in marine areas.

scholarly journals Co-selection of antibiotic and heavy metal resistance in freshwater bacteria

2016 ◽  
Vol 75 (s2) ◽  
Author(s):  
Andrea Di Cesare ◽  
Ester Eckert ◽  
Gianluca Corno
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Resistant Bacteria ◽  
Human Pathogens ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Selection Of

<p class="p1">Antibiotic resistant bacteria are found in most environments, especially in highly anthropized waters. A direct correlation between human activities (<em><span class="s1">e.g., </span></em>pollution) and spread and persistence of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) within the resident bacterial communities appears more and more obvious. Furthermore, the threat posed for human health by the presence of ARB and ARGs in these environments is enhanced by the risk of horizontal gene transfer of resistance genes to human pathogens. Although the knowledge on the spread of antibiotic resistances in waters is increasing, the understanding of the driving factors determining the selection for antibiotic resistance in the environment is still scarce. Antibiotic pollution is generally coupled with contamination by heavy metals (HMs) and other chemicals, which can also promote the development of resistance mechanisms, often through co-selecting for multiple resistances. The co-selection of heavy metal resistance genes and ARGs in waters, sediments, and soils, increases the complexity of the ecological role of ARGs, and reduces the effectiveness of control actions. In this mini-review we present the state-of-the-art of the research on antibiotic- and HM-resistance and their connection in the environment, with a focus on HM pollution and aquatic environments. We review the spread and the persistence of HMs and/or ARB, and how it influences their respective gene co-selection. In the last chapter, we propose Lake Orta, a system characterized by an intensive HM pollution followed by a successful restoration of the chemistry of the water column, as a study-site to evaluate the spread and selection of HMs and antibiotic resistances in heavily disturbed environments.</p>

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

Heavy Metal Resistance by Endophytic Bacteria Isolated from Guava (Psidium Guajava) and Mango (Mangifera Indica) Leaves

2019 ◽  
Vol 9 (1) ◽  
pp. 16-23
Author(s):  
Maryam Lami Riskuwa-Shehu ◽  
Haruna Yahaya Ismail ◽  
Udem Joshua Josiah Ijah
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Endophytic Bacteria ◽  
Mangifera Indica ◽  
Bacterial Species ◽  
Heavy Metal Resistance ◽  
Psidium Guajava ◽  
Metal Resistance ◽  
Resistant Bacteria ◽  
Mango Leaves

Heavy metal resistant bacteria are widespread in nature and their application in decontamination of polluted ecosystems is promising. In this study, ability of endophytic bacteria isolated from Psidium guajava (Guava) and Mangifera indica (Mango) for heavy metal resistance was assessed. Leaves samples form the two plants were collected and processed according to the standard laboratory practices. Heavy metals were analyzed using Atomic absorption spectrophotometer. Endophytic bacteria were isolated and identified using morphological and biochemical characteristics; heavy metal resistance was determined by plate dilution method. Heavy metal analysis revealed that the leaves samples contained considerable quantities of Manganese (Mn), Lead (Pb) and Cadmium (Cd) ranging from 1.21±1.6 mg/Kg (for Cd in Guava leaves) to 116.58±1.3 mg/Kg (for Mn in Mango leaves). A total of six bacterial species were isolated from both of the plants leaves (3 each). Guava endophytes were identified as Streptococcus sp, Staphylococcus albus and Staphylococcus seiuri whereas Staphylococcus aureus, Staphylococcus xylulose and Staphylococcus intermedius were from Mango leaves. The identified isolates were tested for ability to resist heavy metals in-vitro and were capable of showing different patterns of resistance to MnCl2, PbCl2 and CdCl2.  All the endophytes were highly resistant to PbCl2 followed by MnCl2 but susceptible to CdCl2. The ability of plants and bacterial endophytes understudy to tolerate or resist heavy metals is a good indication of their phytoremediation potentials and thus, should be harnessed.

Ekplorasi dan Karakterisasi Biokimia Bakteri Resisten Timbal (Pb) dari Sungai Tallo Makassar

2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Fahruddin Fahruddin ◽  
Nur Haedar ◽  
Slamet Santosa ◽  
Sri Wahyuni
Keyword(s):  
Heavy Metal ◽  
Water Samples ◽  
Bacterial Isolate ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Sediment Samples ◽  
Agar Media ◽  
Biological Methods ◽  
Selection Of

Industrial progress and waste of urban domestic activities have an impact on the Pb heavy metal pollution on the environment, this will have an impact on human health. Pb metal can be overcome with biological methods by utilizing bacteria in reducing Pb metal. The study aimed to obtain the species  of resistant bacterial isolates of Pb metal and determine its characteristics. Sediment and water samples were obtained from the Tallo River. Isolation and selection of Pb metal resistant bacteria were carried out on nutrient agar media is 10 ppm added of PbAgNO3, afterwards the colonies that grew and differed were characterized by morphological morphology and several biochemical tests. The characterization results obtained 8 isolates of Pb metal resistant bacteria consisted of 8 isolates from sediment samples and 3 isolates from water samples. The characteristics of each bacterial isolate on cell morphology are the same and biochemical tests show different results.

scholarly journals Antibiotic and Heavy Metal Resistance of Bacteria Isolated from Diseased Mud Crab (Scylla serrata)

2015 ◽  
Vol 3 (3) ◽  
pp. 1-15
Author(s):  
Cik Syahrizawati M Z. ◽  
Ikhwanuddin M. ◽  
Wendy W. ◽  
Zulhisyam AK ◽  
Lee SW
Keyword(s):  
Heavy Metal ◽  
Vibrio Parahaemolyticus ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Edwardsiella Tarda ◽  
Scylla Serrata ◽  
Bacterial Isolates ◽  
Mud Crab ◽  
Salmonella Spp ◽  
Klebsiella Spp

A total of 320 bacteria isolated from marketable size and diseased mud crab (Scylla serrata) at a commercial farm. The isolated bacteria were Aeromonas spp. n = 70, Edwardsiella tarda n = 50, Vibrio alginolyticus n = 40, Vibrio parahaemolyticus n = 20, Salmonella spp. n = 70 and Klebsiella spp. n = 70. All the bacterial isolates were tested for antibiotic susceptibility against 16 types of antibiotics by using disk diffusion method. The antibiotics tested in this study were nalidixic acid (30 ?g/disk), oxolinic acid (2 ?g/disk), compound sulphonamides (300 ?g/disk), doxycycline (30 ?g/disk), tetracycline (30 ?g/disk), novobiocin (30 ?g/disk), chloramphenicol (30 ?g/disk), kanamycin (30 ?g/disk), sulphamethoxazole (25 ?g/disk), flumequine (30 ?g/disk), erythromycin (15 ?g/disk), ampicillin (10 ?g/disk), spiramycin (100 ?g/disk), oxytetracycline (30 ?g/disk), amoxycillin (25 ?g/disk) and fosfomycin (50 ?g/disk). Heavy metal resistance pattern of the present bacterial isolates was also characterized against mercury (Hg2+), chromium (Cr6+), copper (Cu2+), and Zinc (Zn2+) by using two fold agar dilution method. The percentage of antibiotic sensitivity of the present bacterial isolates was ranged from 12.5 % to 100 % in which most of the present bacteria isolates were not sensitive to ampicillin whereas all the bacteria isolates were sensitive to nalidixic acid, flumequine and oxytetracycline. Overall, the total of antibiotic sensitive case was reported as 72.7% whereas antibiotic resistance and intermediate sensitive case was recorded as 19.7% and 7.4 %, respectively. The Multiple Antibiotic Resistance (MAR) values were range of 0.03 to 0.29 in which Aeromonas spp (0.29) showed the highest value of MAR. This was followed by Salmonella spp. (0.21) and Klebsiella spp. (0.21), Edwardsiella tarda (0.20), Vibrio alginolyticus (0.09) and Vibrio parahaemolyticus (0.03). The MAR value indicated that the commercial S. serrata were not contaminated to the test antibiotics. Furthermore, low resistance activity of the present bacterial isolates to the tested heavy metals (Cr6+: 20.7 % to 30.8 %, Zn2+: 0 % to 40 %, Cu2+: 18.8 % to 25 % and Hg2+: 30 % to 33.3 %) was observed.

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