Immunomolecular Expression of TLR1 and TLR2 Genes Related With Prostatitis
Determine Immunogenetic expression of Toll-like receptor gene clusters related to prostatitis was carried in this investigation to give acknowledge about Role of TLR in prostatitis immunity in men. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected , The molecular expression study include extracting DNA from blood of Prostatitis patients, Prostitis patients and Control group by using specific primers for conventional PCR and Real Time PCR , the amplification of all extracted DNA from blood samples was preform and confirm by using electrophoresis with (100volt/30min). The estimation revealed that the amplified DNA (PCR product) was 135bp for TLR1 (at 50 voltage for 1hour) with the presence of TLR1 was 75% of total samples, and for TLR2 the PCR product was 125bp on agarose gel (1%), (50volt/1hour) with a presence of 95% from total samples. The results of the present study indicate that the Toll like receptor alleles associated with risk of prostatitis.