STAPHYLOCOCCUS AUREUS RESISTANCE AGAINST CEPHALOSPORIN ANTIBIOTIC IN ADEN-YEMEN

The study was carried out to determine antibacterial resistance profiles of methicillin-resistant Staphylococcus aureus (MRSA), isolated from clinical samples (n: 352) of patients during the period, from January 2019 to July 2020 in five governmental and private medical laboratories of Aden governorate, Yemen. The results showed the percentage for resistance ratio is differentiated between samples and the category of cephalosporin antibiotic groups. The highest percentage of resistance was in the wound sample for Cefadroxil, Cefuroxime, and Ceftriaxone at (100%), in addition to 100% in the pus, CSF, and sputum samples for Ceftriaxone antibiotic. Moreover, Cefadroxil has 100 % of resistance in semen, ear, and sputum samples. However, the Staphylococcus aureus isolated from the pharyngeal sample not showed any resistance to all cephalosporin antibiotic groups.

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EVALUATION OF SOME ANTIBIOTIC RESISTANCE IN STAPHYLOCOCCUS AUREUS ISOLATED BY MEDICAL LABORATORIES ADEN, YEMEN

Electronic Journal of University of Aden for Basic and Applied Sciences ◽

10.47372/ejua-ba.2021.1.89 ◽

2021 ◽

Vol 2 (1) ◽

pp. 49-53

Author(s):

Eman Abdo Ali ◽

Omniat N. M. Alshuaibi ◽

Khaled Saeed Ali

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistance ◽

Medical Laboratory ◽

Diffusion Method ◽

Clinical Samples ◽

Urine Specimen ◽

Antibacterial Resistance ◽

Disc Diffusion Method ◽

Medical Laboratories

The study was carried out to determine antibacterial resistance profiles of methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical samples of patients during the duration, from January 2019 to July 2020 in five governmental and private medical laboratories of Aden governorate, Yemen. A total of 267 MRSA isolates were tested with a disc diffusion method from 4 clinical specimens. The isolates were examined for methicillin resistance using vancomycin, erythromycin, amikacin, and gentamicin. The results revealed a large number of MRSA isolates showed resistance to erythromycin (43.5%) and Vancomycin (28%). A lower number of MRSA isolates showed resistance to amikacin (6.25%), gentamicin (11.75%). A majority of MRSA isolates were from urine specimen (162) followed by (56) wounds, (34) pus, and (25) semen specimens. The highest proportion of MRSA isolates were obtained from Al-medina medical laboratory (31%) and Alborg laboratory (24%).

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Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

Pharmaceuticals ◽

10.3390/ph14050420 ◽

2021 ◽

Vol 14 (5) ◽

pp. 420

Author(s):

Tanveer Ali ◽

Abdul Basit ◽

Asad Mustafa Karim ◽

Jung-Hun Lee ◽

Jeong-Ho Jeon ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Active Site ◽

Methicillin Resistant Staphylococcus Aureus ◽

Meca Gene ◽

Resistance Mechanism ◽

Ligand Docking ◽

Clinical Samples ◽

Allosteric Site ◽

Methicillin Resistant

β-Lactam antibiotics target penicillin-binding proteins and inhibit the synthesis of peptidoglycan, a crucial step in cell wall biosynthesis. Staphylococcus aureus acquires resistance against β-lactam antibiotics by producing a penicillin-binding protein 2a (PBP2a), encoded by the mecA gene. PBP2a participates in peptidoglycan biosynthesis and exhibits a poor affinity towards β-lactam antibiotics. The current study was performed to determine the diversity and the role of missense mutations of PBP2a in the antibiotic resistance mechanism. The methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical samples were identified using phenotypic and genotypic techniques. The highest frequency (60%, 18 out of 30) of MRSA was observed in wound specimens. Sequence variation analysis of the mecA gene showed four amino acid substitutions (i.e., E239K, E239R, G246E, and E447K). The E239R mutation was found to be novel. The protein-ligand docking results showed that the E239R mutation in the allosteric site of PBP2a induces conformational changes in the active site and, thus, hinders its interaction with cefoxitin. Therefore, the present report indicates that mutation in the allosteric site of PBP2a provides a more closed active site conformation than wide-type PBP2a and then causes the high-level resistance to cefoxitin.

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Antibiotic Susceptibility Pattern of Nosocomial Isolates of Staphylococcus aureus in a Tertiary Care Hospital, Nepal

Journal of Nepal Medical Association ◽

10.31729/jnma.190 ◽

2009 ◽

Vol 48 (175) ◽

Cited By ~ 1

Author(s):

Bidya Shrestha ◽

B M Pokhrel ◽

T M Mohapatra

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care ◽

Test Method ◽

University Teaching ◽

Clinical Samples ◽

Resistance Pattern ◽

Care Hospital ◽

Biochemical Tests ◽

Methicillin Resistant

Introduction: Methicillin resistant Staphylococcus aureus (MRSA), the most common cause ofnosocomial infection has been a major cause of morbidity and mortality around the world. They arenormally resistant to most of the antibiotics used in clinical practice. This study has been carried outto fi nd out the resistance pattern among S. aureus.Methods: During November 2007 to June 2008, clinical samples from patients with nosocomialinfection were processed for culture and sensitivity following standard methodology in microbiologylaboratory, Tribhuvan University teaching hospital, Kathmandu, Nepal.Results: Among 149 Staphylococcus aureus isolates, highest resistance was observed against Penicillin(91.94%) followed by Fluoroquinolone (61.74%), Erythromycin (52.94%), Gentamicin (46.98%),Cotrimoxazole (42.95%), Tetracycline (40.94%) and others, whereas susceptibility was observedmaximum against Chloramphenicol (94.85%) followed by Rifampicin (92.61%), Tetracycline(59.06%), Cotrimoxazole (57.04%), and others. None of the isolates were resistant to Vancomycinand Teicoplanin. Of these isolates 44.96 % of the isolates were Methicillin resistant S. aureus (MRSA).Resistance to Penicillin, Fluoroquinolone, Erythromycin, Gentamicin, Co-trimoxazole and Tetracyclinewere associated signifi cantly with MRSA isolates (X2= 8.779, p<0.05, X2= 74.233, p<0.05, X2= 84.2842,p<0.05, X2= 108.2032, p<0.05, X2= 88.1512, p<0.05 and X2= 79.1876, p<0.05 respectively). Althoughmost of the Methicillin sensitive S. aureus (MSSA) isolates were susceptible to both Rifampicinand Chloramphenicol, only Rifampicin susceptibility was signifi cantly associated with them (X2=10.1299, p<0.05). Among three Biochemical tests for the detection of β lactamase detection namelychromogenic, iodometric and acidimetric test, chromogenic test method had highest sensitivity andspecifi city.Conclusions: Since MRSA comprised a greater part of S. aureus isolates and were multi-resistant,patients infected by such strains should be identifi ed and kept in isolation for hospital infectioncontrol and treated with second line of drug like vancomycin.Key Words: β lactamase, methicillin resistant Staphylococcus aureus,methicillin sensitive Staphylococcus aureus, resistance pattern

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Characterization of Methicillin Resistant Staphylococcus aureus from various clinical samples at tertiary care hospital of rural Gujarat

Journal of Research in Medical and Dental Science ◽

10.5455/jrmds.20142311 ◽

2014 ◽

Vol 2 (3) ◽

pp. 49 ◽

Cited By ~ 3

Author(s):

Nirav Pandya ◽

Anil Chaudhary ◽

Sanjay Mehta ◽

Rosy Parmar

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Clinical Samples ◽

Care Hospital ◽

Methicillin Resistant

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Antibiotic resistance pattern of Staphylococcus aureus with reference to MRSA isolates from pediatric patients

10.21203/rs.2.10785/v1 ◽

2019 ◽

Author(s):

Raja Ram Gurung ◽

Prashanna Maharjan ◽

Ganga GC

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Pediatric Patients ◽

Methicillin Resistant Staphylococcus Aureus ◽

Current Status ◽

Clinical Samples ◽

Resistance Pattern ◽

Isolation And Identification ◽

Cross Sectional ◽

Methicillin Resistant

Abstract Background: Staphylococcus aureus is one of the important superbugs distributed throughout the world. It causes minor skin infections to severe complications including nosocomial infections in both hospitals and community settings. These strains have multi-drug resistant property. Hence, they are difficult to manage which increase health-related costs and simultaneously intensifying the need for new antibiotics. The extent of Methicillin-Resistant Staphylococcus aureus (MRSA) in children is largely unknown. The study determines the current status of S. aureus and MRSA causing various infections in pediatric patients visiting International Friendship Children’s Hospital (IFCH). Methods: A cross-sectional study was conducted among patients visiting a hospital. Various clinical specimens were aseptically collected and processed according to standard microbiological procedures. Isolation and identification of S. aureus were done by microscopy, mannitol fermentation, and coagulase positivity. All identified S. aureus isolates subjected to in-vitro antibiogram by Kirby-Bauer disc diffusion technique adopting Clinical and Laboratory Standards Institute (CLSI) guideline. Isolates resistant to cefoxitin were considered to be MRSA. Whereas, isolates produced D-shaped inhibition zone around clindamycin when kept near erythromycin were considered to be Inducible Clindamycin Resistant (ICR). Results: 672 various types of clinical samples were processed from the microbiology laboratory from June and November 2015. Out of 300 culture positive samples, 52 (17.3%) were S. aureus isolates, among them 39 (75.0%) were found to be MRSA. The D-test showed that Macrolide-Lincosamide-Streptogramin-B (MLSB) phenotype was 15.4%. Conclusion: The study shows the MRSA occurrence is prevalent in pediatric patients and newer classes’ drugs are found more effective than β-lactam drugs to treat S. aureus infection. However, restriction on the indiscriminate use of such drugs may be an effective strategy to control the drug resistance. Keywords: Methicillin-Resistant Staphylococcus aureus (MRSA), Macrolide-Lincosamide-Streptogramin B (MLSB) phenotype, Inducible Clindamycin Resistant (ICR) test or D-zone test, Antibiotic resistance, Nepal

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