scholarly journals Effect of Aspirin on Experimental Atherosclerosis in Rabbits Submitted to Hypercholesterolemic Diet

2021 ◽  
Keyword(s):  
Protective Effect ◽  
Total Cholesterol ◽  
White Male ◽  
Atherosclerotic Lesion ◽  
Experimental Atherosclerosis ◽  
Hypercholesterolemic Diet ◽  
Normal Chow ◽  
Group A ◽  
Sudan Iii

Aspirin, besides its antithrombotic activity, has also been quoted for protective effect reducing new coronary lesions. Objective: Considering the growing interest in the study of drugs that prevent the progression of atherosclerotic lesion, the objective of this study was to determine the effect of aspirin in experimental atherogenesis induced in rabbits fed with cholesterol rich diets. Method: Thirty adult, New Zealand white male rabbits, with 3.4 kg of body weight were submitted to a 1.5% cholesterol-rich-diet for 9 weeks. Aspirin was triturated, mixed with chloroform and incorporated to the normal chow. The drug was given to the rabbits every day in portions of 20 g of chow with 100 mg of aspirin. The rabbits were divided in 3 groups as follows: A) Cholesterol-rich-diet (n=10); B) Cholesterol-rich-diet plus aspirin (n=10) and C) Normal chow (n=10). Blood samples were collected before starting the diet, at 5 weeks and at sacrifice (9 weeks) for determination of total cholesterol, triglycerides, enzymes and hematological tests. After sacrifice, staining of the aorta was done by Sudan III for visualization of sudanophillic plaques. The percentual of aorta couvered with lipidic deposits were determined by computerized planimetry. Results: Total cholesterol (mg/dl) was per group: A- TO=52, T5=424 and T9=1.483; B- TO=32, T5=755 and T9=1.436; C- TO=41, T5=22 and T9=27. Planimetry data did not differ among groups A (23.3%) and B (27.3%). Scanning microscopy – the interpretation of the document images in three groups showed insignificant platelet deposition in all aortic segments both in groups B and C. Conclusion: This experiment enhances the theoretic basis for the protective effect of aspirin as an antiaggregant factor in the experimental aortic atherosclerotic lesion.

scholarly journals Individual housing influences certain biochemical parameters in the rat

1997 ◽  
Vol 31 (4) ◽  
pp. 357-361 ◽  
Author(s):  
C. Pérez ◽  
J. R. Canal ◽  
E. Domínguez ◽  
J. E. Campillo ◽  
M. Guillén ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Biochemical Parameters ◽  
Plasma Triglyceride ◽  
Female Rats ◽  
Individual Housing ◽  
Group A ◽  
Food And Water Intake ◽  
Group B ◽  
The Individual

Individual housing has been reported to modify animal behaviour. The present study compares the plasma levels of glucose, triglycerides and total cholesterol, weight, and food and water intake in two groups of female rats. Group A: 10 rats who remained grouped in two cages for 21 days; and Group B: 10 rats housed in two cages for 7 days, then isolated in individual cages from day 8 to day 15, and finally grouped together again for the last 7 days of the study. The results showed that the plasma values of glucose declined ( P<0.05) in the Group B rats when they had been returned to group condition (4.79±0.72 mM) than when they had been isolated (5.45±0.94 mM). Plasma triglyceride levels were lower ( P<0.05) in isolated rats (0.70±0.26 mM) than in any determination of the grouped rats. Group B: 1st week 1.21±0.21 mM, 3rd week 0.88±0.20 mM; and Group A: 1.22±0.20, 0.96±0.16, and 0.96±0.36 mM, in the first, second, and third week, respectively. There were no statistically significant differences in total cholesterol values as a function of the individual housing of animals. While there was no weight difference between the two groups of rats that could be ascribed to individual housing, there was a statistically significant increase ( P<0.05) in the food intake of isolated rats (17.5±3.2 g) with respect to values in the same Group B animals when they were housed together (1st week, 16.6±2.8 g; 3rd week, 16.8±3.1 g). These results therefore confirm that individual housing of female rats provoke variations in certain biochemical parameters, and that if this is not taken into account in performing different scientific studies, it could give rise to unreliable or even dubious results.

Polarographic and spectrophotometric behaviour of some N-p-phenyl substituted benzamidines

1991 ◽  
Vol 56 (12) ◽  
pp. 2791-2799 ◽  
Author(s):  
Juan A. Squella ◽  
Luis J. Nuñez-Vergara ◽  
Hernan Rodríguez ◽  
Amelia Márquez ◽  
Jose M. Rodríguez-Mellado ◽  
...  
Keyword(s):  
Spectrophotometric Study ◽  
Absorption Bands ◽  
Electrochemical Parameters ◽  
Wide Ph Range ◽  
Group A ◽  
Substituent Constants ◽  
Ph Range ◽  
The Waves

Five N-p-phenyl substituted benzamidines were studied by DC and DP polarography in a wide pH range. Coulometric results show that the overall processes are four-electron reductions. Logarithmic analysis of the waves indicate that the process are irreversible. The influence of the pH on the polarographic parameters was also studied. A UV spectrophotometric study was performed in the pH range 2-13. In basic media some variations in the absorption bands were observed due to the dissociation of the amidine group. A determination of the pK values was made by deconvolution of the spectra. Correlations of both the electrochemical parameters and spectrophotometric pK values with the Hammett substituent constants were obtained.

scholarly journals DETERMINATION OF FREE AND TOTAL CHOLESTEROL BY DIRECT CHLOROFORM EXTRACTION

1949 ◽  
Vol 180 (1) ◽  
pp. 315-328 ◽  
Author(s):  
George R. Kingsley ◽  
Roscoe R. Schaffert
Keyword(s):  
Total Cholesterol ◽  
Chloroform Extraction

scholarly journals Hematological Features in Sheep with IgG and IgM Antibodies against Borrelia burgdorferi sensu lato

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 164
Author(s):  
Labrini V. Athanasiou ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Panagiotis D. Katsoulos
Keyword(s):  
Borrelia Burgdorferi ◽  
Rural Areas ◽  
Blood Analysis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Igm Antibodies ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Immunofluorescence Antibody

Exposure of sheep to Borreliaburgdorferi sensulato (s.I.) complex, the causative agent of Lyme borreliosis (LB), has been reported in tick-abundant areas worldwide, while no data have been reported in Greece. The aim of the study was to identify the hematological alterations in sheep with seropositivity against Borrelia burgdorferi (s.I.). Blood samples were obtained from 318 tick infested sheep for blood analysis and serological determination of IgG and IgM antibodies against B. burgdorferi by indirect immunofluorescence antibody (IFA) assay after exclusion of endo-ectoparasites and other tick-borne infections. A total number of 162 sheep met the inclusion criteria, allocated in four groups based on the presence or absence of IgG and/or IgM; sheep found negative for IgM and IgG (Group A), positive for IgM (Group B), positive for both IgM and IgG (Group C) and positive for IgG (Group D). Anemia, thrombocytopenia and normal or decreased leukocyte count, mainly due to lymphopenia were the main hematological features observed in seropositive sheep. The presence of these features raises the suspicion of Borrelia infection in tick infested sheep. The seropositivity of 23.58% in sheep raises concerns of Borrelia circulation, especially in rural areas and potential risk of transmission to humans.

scholarly journals Computer aided determination of a Fibonacci group

1976 ◽  
Vol 15 (2) ◽  
pp. 297-305 ◽  
Author(s):  
George Havas
Keyword(s):  
Computer Aided ◽  
Group A ◽  
Coset Enumeration

The Fibonacci group F(2, 7) has been known to be cyclic of order 29 for about five years. This was first established by computer coset enumerations which exhibit only the result, without supporting proofs. The working in a coset enumeration actually contains proofs of many relations that hold in the group. A hand proof that F(2, 7) is cyclic of order 29, based on the working in computer coset enumerations, is presented here.

EVALUATION OF PROTEIN IN FOODS: IV. A SIMPLIFIED CHEMICAL SCORE

1959 ◽  
Vol 37 (1) ◽  
pp. 1293-1299 ◽  
Author(s):  
J. M. McLaughlan ◽  
C. G. Rogers ◽  
D. G. Chapman ◽  
J. A. Campbell
Keyword(s):  
Rapid Screening ◽  
Assay Method ◽  
Efficiency Ratio ◽  
Lysine Concentration ◽  
Chemical Score ◽  
Group A ◽  
High Degree ◽  
The Relationship ◽  
Bio Assay

Available evidence has shown that most common foods are deficient in lysine, methionine, or in methionine and cystine. Based on the determination of these amino acids, a simplified chemical score was developed and compared with protein efficiency ratio (P.E.R.) values determined with the same samples. Each of 43 foods was assigned to either of two categories: (a) foods apparently deficient in lysine or (b) in methionine (+ cystine). With 16 foods in the former group there was a high degree of correlation between lysine concentration and P.E.R. With 27 foods in the latter group, a good correlation was found between the methionine (+ cystine) concentration and P.E.R. Since the regression lines for the two groups were different, a factor was added to the methionine (+ cystine) values to simplify the relationship. That foods deficient in lysine or in methionine (+ cystine) fell into two distinct groups appeared to be substantiated by data available in the literature. Because the simplified method is relatively rapid, yields reproducible results, and correlates with animal assays, it is proposed as a rapid screening procedure for the evaluation of protein in foods, but is not intended to replace the rat bio-assay method.

scholarly journals Clinical Efficacy Of Ceftriaxone Versus Ciprofloxacin In Paediatric Enteric Fever

2018 ◽  
Vol 08 (02) ◽  
pp. 67-71
Author(s):  
Anila Farhat ◽  
Asma Shaukat ◽  
Tariq Mahmood Khan
Keyword(s):  
Enteric Fever ◽  
Research Study ◽  
Controlled Trial ◽  
Clinical Effectiveness ◽  
Randomized Controlled ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Objective Determination

Objective: Determination of the clinical effectiveness of ciprofloxacin versus ceftriaxone in children with enteric fever on the basis of defervescence within 72 hours of commencement of treatment. Duration and Place of Study: This randomized controlled trial was carried out from 15th May to 15th November 2017 in pediatrics units of Benazir Bhutto Shaheed Teaching Hospital Abbottabad and Jinnah International Hospital Abbottabad. Methodology: 90 children with uncomplicated enteric fever were admitted and divided randomly into two groups, Group A was administered I/V Ceftriaxone 75mg/kg OD and Group B was given I/V Ciprofloxacin 10mg/kg BD for seven days. Response to drug was taken as defervescence within 72 hours while continued fever after 72 hours was taken as no response. The data was analyzed by using SPSS Version 21.00. Results: In our research study 53(58.9%) children were male and 37(41.1%) patients were female. The mean age was 8.43±3.17 years encompassing 4 to 14 years, mean weight of the patients in kg was 29.54±10.8 kg. Efficacy of ceftriaxone group was 93.3% while in ciprofloxacin group 62.2% patients became afebrile in 72 hours. The proportion of achieving defervescence within 72 hours was higher with ceftriaxone than with ciprofloxacin Conclusion: Ceftriaxone is more efficacious in terms of achieving defervescence than ciprofloxacin in children having enteric fever.

scholarly journals The Role of Pathological Method and Clearance Definition for the Evaluation of Margin Status after Pancreatoduodenectomy for Periampullary Cancer. Results of a Multicenter Prospective Randomized Trial

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2097
Author(s):  
Gennaro Nappo ◽  
Domenico Borzomati ◽  
Alessandro Zerbi ◽  
Paola Spaggiari ◽  
Ugo Boggi ◽  
...  
Keyword(s):  
Prognostic Factor ◽  
Randomized Study ◽  
Periampullary Cancer ◽  
Significant Prognostic Factor ◽  
Prognostic Role ◽  
R1 Resection ◽  
Group A ◽  
Group B

Background: There is extreme heterogeneity in the available literature on the determination of R1 resection rate after pancreatoduodenectomy (PD); consequently, its prognostic role is still debated. The aims of this multicenter randomized study were to evaluate the effect of sampling and clearance definition in determining R1 rate after PD for periampullary cancer and to assess the prognostic role of R1 resection. Methods: PD specimens were randomized to Leeds Pathology Protocol (LEEPP) (group A) or the conventional method adopted before the study (group B). R1 rate was determined by adopting 0- and 1-mm clearance; the association between R1, local recurrence (LR) and overall survival (OS) was also evaluated. Results. One-hundred-sixty-eight PD specimens were included. With 0 mm clearance, R1 rate was 26.2% and 20.2% for groups A and B, respectively; with 1 mm, R1 rate was 60.7% and 57.1%, respectively (p > 0.05). Only in group A was R1 found to be a significant prognostic factor: at 0 mm, median OS was 36 and 20 months for R0 and R1, respectively, while at 1 mm, median OS was not reached and 30 months. At multivariate analysis, R1 resection was found to be a significant prognostic factor independent of clearance definition only in the case of the adoption of LEEPP. Conclusions. The 1 mm clearance is the most effective factor in determining the R1 rate after PD. However, the pathological method is crucial to accurately evaluate its prognostic role: only R1 resections obtained with the adoption of LEEPP seem to significantly affect prognosis.

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