A Laboratory Manual for the Preparation of Chemical Reagents, Solutions and Special Indicators

A Laboratory Manual for the Preparation of Chemical Reagents, Solutions and Special Indicators. ETHIOPIAN BIODIVERSITY INSTITUTE, Microbial Biodiversity Directorate. This manual includes detailed view of the following: Laboratory Safety Precautions, Chemical Reagent Preparation, Buffer and Chemical Solution Preparation, Staining Solution Preparation, Indicators and Dye Solution Preparation, Preparation of Special Reagents, Known and Suspected List of Carcinogens and References.

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The Preparation of Chlorinated Rubbers from Latex. Hypochlorination and Chlorination Processes

Rubber Chemistry and Technology ◽

10.5254/1.3548000 ◽

1934 ◽

Vol 7 (2) ◽

pp. 320-326 ◽

Cited By ~ 6

Author(s):

G. F. Bloomfield ◽

E. H. Farmer

Keyword(s):

Limited Range ◽

Rubber Latex ◽

Chemical Reagent ◽

Direct Introduction ◽

Chemical Reagents ◽

Ph Values

Abstract With the view of extending the field of application of rubber latex, it has been considered desirable to study the behavior of this material toward various chemical reagents. Owing to the colloidal nature of latex the choice of reagent is considerably restricted; acids and many salts (especially those of bivalent and tervalent metals) produce coagulation, while alkaline reagents may cause creaming and aggregation of the particles. It is known that with the aid of certain stabilizers (e. g., saponin or casein) a stable acid latex may be obtained, but this is in general only stable over a limited range of pH values and is not stable to heat; moreover the stabilizer itself is prone to attack by the chemical reagent introduced. As would be anticipated from the foregoing considerations, the direct introduction of chlorine into latex immediately causes coagulation. Ellis and Boehmer (U. S. Patent 1,544,535) appear to have achieved some measure of success in chlorinating latex by using liquid chlorine under pressure, but the products obtained in different operations varied considerably in their properties.

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Development of Retrieve/Storage Cabinets for Chemical Reagents

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.577.457 ◽

2014 ◽

Vol 577 ◽

pp. 457-460

Author(s):

Hang Wei Zhang ◽

Jian Feng Huang

Keyword(s):

Management Information System ◽

Storage Device ◽

Application Software ◽

Single Chip ◽

Mechatronic System ◽

Management Information ◽

Single Chip Microcomputer ◽

Chemical Reagent ◽

Chemical Reagents ◽

The Internet Of Things

To integrate chemical reagent retrieve/storage (R/S) device with management information system, an intelligent mechatronic system is developed. Three kinds of unit cabinets for reagent bottles are constructed. A Single-chip Microcomputer and a Personal Computer are served as a slave and host control system respectively. A set of special application software is programmed by VC++ and Access to accomplish reagent retrieve/storage, usage reports and surplus warning by short massage based on the Internet of Things. The integration of chemical reagent R/S device with MIS is realized which could make the storage device automation, data visualization and measurement precision.

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Study on Intermolecular Forces of Corn Gluten with the Chemical Reagent Treatment

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1049-1050.547 ◽

2014 ◽

Vol 1049-1050 ◽

pp. 547-550

Author(s):

Rong Yan ◽

Xin Hua Li ◽

Xiao Jun Qi

Keyword(s):

Microscopic Observation ◽

Starch Granule ◽

Intermolecular Forces ◽

Small Granule ◽

Intermolecular Hydrogen Bonds ◽

Chemical Reagent ◽

Chemical Reagents ◽

Corn Gluten ◽

Reagent Treatment ◽

Observation Results

Corn gluten were steeped with different chemical reagents, 2%urea, 5%tween80, 5% NaCl, 2%L - cysteine, using distilled water as control. Intermolecular forces in corn gluten under different reagents were analyzed using the amount of free starch measuring by iodine calorimetry. By the light microscope, intermolecular structure transformation in corn gluten was analyzed before the treatment and after. The results show that Urea, twain, NaCl, and L-cysteine all can make the starch that closely combined with protein migrate away, in which the effect of L-cysteine and NaCl were better, releasing more free starch. It is found through microscopic observation that the starch granule existing in corn gluten is small granule from cutin endosperm, and microscopic observation results consistent with the free starch results. By analysis of the amount of free starch and the microscopic structure change of corn gluten and the mechanism of measuring intermolecular forces with the chemical reagent, it is demonstrated that disulfide bond and electrostatic attraction were main intermolecular forces in corn gluten, which was the main reason for the search combining with the protein .The effect of intermolecular hydrogen bonds and hydrophobic interaction were weaker.

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On the prevention of the black spot of shrimp: I. effect of some chemical reagents on the black spot of shrimp. II. the isolation and purification of the enzyme related to the formation of black spot

Boletim do Instituto Oceanográfico ◽

10.1590/s0373-55241967000100001 ◽

1967 ◽

Vol 16 (1) ◽

pp. 01-11

Author(s):

Yasuzo Itô

Keyword(s):

Ascorbic Acid ◽

Low Temperatures ◽

Sodium Thiosulfate ◽

Black Spot ◽

Chemical Solution ◽

Isolation And Purification ◽

Chemical Reagents ◽

Initial Extract ◽

Different Temperatures ◽

Black Discoloration

The causes of the black discoloration of three species of shrimp commonly caught off the coast of the State of São Paulo, Brazil, are studied and measures for its prevention are advocated. Landings of shrimp in Santos in 1964 equaled 13% of the total landing, by weight, while economically it is the principal fishery. I - Blackening was effectively prevented by dipping the shrimp in cooled chemical solution for 30 minutes and then storing them at low temperatures. L-ascorbic acid gave the best results, delaying discoloration up to 9 days at 0º to -2ºC and for a longer period at colder temperatures. The effect of Sodium-thiosulfate and EDTA at different temperatures is also discussed. II - Tyrosinase was isolated and assayed by measuring the formation of Dopa-chrome from DL-Dopa at pH 6.8 an 37ºC. Tyrosinase was obtained from the shrimp liver and was purified by approximately 25 times its activity from the initial extract by absorption chromatography through Celite 545, as judged by the rate of dopachrome formation.

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Biocementation Potential of Tropical Residue Soil Infused with Facultative Anaerobe Bacteria

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.773-774.1412 ◽

2015 ◽

Vol 773-774 ◽

pp. 1412-1416 ◽

Cited By ~ 1

Author(s):

Kenny Tiong Ping Chiet ◽

Khairul Anuar Kassim ◽

Siaw Yah Chong

Keyword(s):

Shear Strength ◽

Bacillus Subtilis ◽

Treatment Condition ◽

Soil Improvement ◽

Soil Samples ◽

Residual Soil ◽

Facultative Anaerobe ◽

Chemical Reagent ◽

Chemical Reagents

Biomediated soil improvement, a promising new branch of microbial geotechnology; which involved multi disciplines has successfully attracted abundant attentions among researchers, geotechnical engineers, and other industries practitioners. Few of the researches were conducted to examine the potential implementation of this technique on tropical residue soil. However, the uncertainties outcomes and inconsistency of bio mediated soil improvement, especially on the clayed soil have made this technique remained at the laboratory stage. Therefore, this paper intended to provide better understanding of this technique by investigating the relation between the bacteria, cementation reagents, and tropical residue soil. The residual soil was mixed with facultative anaerobe bacteria, Bacillus Subtilis before it was compacted into a prefabricated PVC mould. The soil samples were treated with different treatment condition such as (1) control or untreated, (2) treated with cementation solution, (3) treated with bacteria only, and (4) treated with bacteria and Cementation reagent. A worth noting finding showed that the sample treated with bacteria and nutrient only has produced the highest increment of shear strength. This phenomenon might have been caused by the effect of the chemical reagent to the mineralogy of residue soil. The presence of the chemical reagents is believed to have weakened the shear strength of the tropical residual soil.

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Laboratory Safety For the Electron and Correlative Microscopist

Microscopy and Microanalysis ◽

10.1017/s1431927600019255 ◽

1999 ◽

Vol 5 (S2) ◽

pp. 1186-1187

Author(s):

S. E. Miller ◽

D. N. Howel

Keyword(s):

Clinical Laboratory ◽

Control Program ◽

Body Fluids ◽

Laboratory Safety ◽

Quality Control Program ◽

Protective Measures ◽

Chemical Reagents ◽

Physical Hazards ◽

Routine Basis ◽

Multistep Process

Laboratory safety in a diagnostic electron microscopy (EM) laboratory that deals with potentially infectious specimens is a complex issue. The risk of infection of laboratory personnel is an obvious concern, particularly in laboratories, such as diagnostic virology laboratories, which handle infected tissues and body fluids on a routine basis. A second hazard is introduced by the multitude of toxic, corrosive, and irritating chemical reagents required for the preparation of samples for EM. Physical hazards inherent in the complex machinery necessary for EM and other forms of microscopy must also not be overlooked. Attention to each of these concerns must be orchestrated over the course of a multistep process that often lasts several days. Success in this endeavor is facilitated by a comprehensive quality control program encompassing personnel, specimens, chemicals, and equipment.The foundation for safe handling of samples in the clinical laboratory is the principle of universal precautions, under which all blood, other body fluids, and tissues are considered potentially infectious and handled using appropriate protective measures.

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