scholarly journals Comparing Bacterial Leakage of Three Intraorifice Barrier Sealing Materials against Enterococcus faecalis and Proteus vulgaris

2021 ◽  
Vol 22 (6) ◽  
pp. 674-679
Author(s):  
Shreya Kohli Khanna ◽  
H Murali Rao ◽  
Nausheen Aga ◽  
Padma Chandra
Keyword(s):  
Enterococcus Faecalis ◽  
Proteus Vulgaris ◽  
Bacterial Leakage ◽  
Sealing Materials

scholarly journals Blattella germánica (Blattodea: Blattellidae) como potencial vector mecánico de infecciones asociadas a la atención en salud (IAAS) en un centro hospitalario de Villavicencio (Meta-Colombia)

Nova ◽  
2016 ◽  
Vol 14 (25) ◽  
pp. 19
Author(s):  
Gloria Isabel Jaramillo ◽  
Norma Cristina Pavas ◽  
Juan Camilo Cárdenas ◽  
Paola Gutiérrez ◽  
William Andrés Oliveros ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Blattella Germanica ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Staphylococcus Xylosus

<p class="Default"> </p><p class="Default"> </p><p>Identificar las especies de cucarachas y bacterias asociadas a su exoesqueleto en un centro hospitalario de la ciudad de Villavicencio (Meta, Colombia). <strong>Métodos. </strong>Se realizaron capturas manuales de cucarachas en cocina, urgencias, UCI intermedia, consulta externa y neonatos. Los individuos col­ectados fueron sometidos a un aislamiento primario en caldo BHI, para luego pasar a medios sólidos (sangre y MacConkey). Se realizó identificación y antibiograma por método automatizado. <strong>Resultados. </strong>Se colectaron 24 ninfas y adultos de <em>Blattella germánica</em>. Se aisló e identificó <em>Klebsiella pneumoniae con </em>sensibilidad intermedia a meropenem (CMI 4) y resistencia a cefalosporinas (cefepima y cefuroxima) (&gt;16), <em>Proteus vulgaris </em>con resistencia a cefalosporinas (ceftriaxona, cefuroxima) (CMI &gt;16), <em>Enterobacter cloacae </em>con resistencia a cefalosporinas (cefoxitina, cefuroxima, ceftriaxona) (CMI &gt; 16), <em>Enterococcus faecium</em>, <em>Enterococcus rafinosus</em>, <em>Staphylococcus xylosus </em>y <em>Enterococcus faecalis</em>. El 80% de las bacterias aisla­das presentaron algún grado de resistencia a antibióticos. <strong>Conclusiones. </strong>Estos insectos podrían jugar un papel importante en la transmisión de las Infecciones asociadas a la atención en salud (IAAS). Debido a su presencia en hospitales y el reporte de IAAS y resistencia bacteriana en varios centros asistenciales en el Departamento del Meta, se hace necesario establecer la relación de estos insectos con estos eventos.</p>

scholarly journals ANTIBIOTICS PRODUCING BACTERIA ISOLATED FROM FARMLANDS

2019 ◽  
Vol 2 (4) ◽  
pp. 99
Author(s):  
Hammed Abiodun Durojaye ◽  
Georgia Chinemenwa Agu
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Bacillus Megaterium ◽  
Pathogenic Bacteria ◽  
Antibiotic Production ◽  
Health Concern ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Ogun State ◽  
Wide Range

The need for new antibiotics has been highlighted recently with the increasing pace of emergence of drug resistance pathogens. Emerging strains of bacteria resistant to most advanced antibiotics have become issues of very important public health concern. Modification of existing antibiotics with the addition of side chains or other chemical group and genomics based drug targeting have been the preferred method of drug development at the corporate level in recent years. In this regard, soil samples were collected from farmlands located in Ibadan in Oyo state, Ago – Iwoye, and Ikenne in Ogun state, Nigeria. Two putative Streptomyces strains and Bacillus strains isolated from the 16 selected farmland soils were characterized and assessed for antibiotic production and activity against a wide range of bacteria including Klebsiella pneumonia, Serratia marscens, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. The extracts of the putative obtained from Streptomyces somaliensis, Streptomyces anulatus, Bacillus megaterium, and Bacillus subtilis showed activities against minimum of 3 and maximum of the 4 of the 7 tested bacteria. Inhibition zones were found to range between 2.0 - 25.0 mm diameters at a concentration of 1ml. The minimum inhibitory concentrations (MICs) of the crude extracts against the tested organisms ranged from 50% and above. Bacillus megaterium, and Streptomyces somaliensis were found to inhibit all the pathogenic bacteria, while S. anulatus was unable to inhibit Proteus vulgaris and Staphylococcus aureus, and B. Subtilis was unable to inhibit Enterococcus faecalis.

scholarly journals Influence of Two Root Canal Obturation Techniques with Resin Based Sealer to Enterococcus faecalis Penetration

2019 ◽  
Vol 1 (2) ◽  
pp. 55
Author(s):  
Aryadi Subrata ◽  
Anastasia Elsa Prahasti ◽  
Bernard Ongki Iskandar
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Root Canal Treatment ◽  
Root Canal Filling ◽  
Gutta Percha ◽  
Single Cone ◽  
Ah Plus ◽  
Bacterial Leakage ◽  
Warm Vertical Compaction

Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment. Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique. Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis. Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus). Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.

scholarly journals Study the Antimicrobial effect of Kombucha tea on bacteria isolated from Diabetic foot ulcer

2014 ◽  
Vol 8 (4) ◽  
pp. 27-33
Author(s):  
E. J. AL-Kalifawi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Staphylococcus Epidermidis ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Kombucha Tea

The study was conducted from January to March 2012. In this study colorimetric VITEK-2 Compact system used for its accuracy and rapidity to identify isolates and to detect several antimicrobial resistances.The study also investigate the antibacterial effect of Kombucha tea on isolated bacteria from diabetic foot ulcer. The bacteria isolated were eight gram negative bacteria, namely, Acinetobacter baumannii 3 (2%), Enterobacter cloacae 5 (4%), Escherichia coli 13 (10%), Klebsiella pneumoniae 7 (6%), Citrobacter spp. 4 (3%), Proteus mirabilis 3 (2%), Proteus vulgaris 3 (2%) and Pseudomonas aeruginosa 44 (35%). Four gram positive bacterium, Enterococcus faecalis 6 (5%), Staphylococcus aureus 17 (13%), Staphylococcus epidermidis 13 (10%) and Streptococcus spp. 9 (8%). The antimicrobial activities of antibiotics showed that, all isolates are sensitive to Ciprofloxacin, Levofloxacin and Ofloxacin. The resistance to other types of antimicrobial differ with different isolate. The effect of Kombucha tea on all isolates wasclear at 7days of incubation; the diameter of inhibition was 6mm for Acinetobacter baumannii, Proteus vulgaris and Enterococcus faecalis. 7mm for Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Citrobacter spp., Staphylococcus epidermidis and Streptococcus spp. 8mm for Proteus mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus. The maximum activity of fermented tea was recorded at 14days incubation of Kombucha organism against all isolates, the diameter of inhibition was 21mm for Acinetobacter baumannii, 24mm for Enterobacter cloacae, 23mm for Escherichia coli, Staphylococcus epidermidis and Streptococcus spp., 16mm for Klebsiella pneumoniae, 22mm for Citrobacter spp. and Enterococcus faecalis, 25mm for Proteus mirabilis and Staphylococcus aureus, 20mm for Proteus vulgaris, 26mm for Pseudomonas aeruginosa. The antibacterial activity of Kombucha tea decrease with increase incubation periods28 days.

scholarly journals Influence of some antibiotics and sulfonamides on the microbiological background of the Baikal seal

2020 ◽  
Vol 5 (4) ◽  
pp. 364-373
Author(s):  
Anna A. Pliska ◽  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Clostridium Perfringens ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Staphylococcus Warneri ◽  
Treatment Measures

The results of microbiological studies of the material taken from the diseased seal are presented. The taxonomic characteristics of microorganisms, their virulence and antibiotic resistance were studied. Treatment measures were developed and performed. It was found that the seal has the following types of microorganisms: Staphylococcus aureus; Enterococcus faecalis; Escherichia coli; Staphylococcus warneri; Proteus vulgaris; Clostridium perfringens, Proteushauseri, Staphylococcus epidermidis, Plesiomonas shigel-loides. All pathogens isolated from the seals were sensitive to 60.3% of antibiotics (ampicillin, cefuroxime, cefixime, gentamicin, amikacin, ciprofloxacin, ofloxacin, furadonin, Ceftriaxone, vancomycin) and resistant to 39.7% of antibiotics, two representatives of cephalosporins (cefaclor and ceftazidim) and ticarcillin.

scholarly journals JONIZUOTO RŪGŠTINIO VANDENS ANTIMIKROBINIS AKTYVUMAS

2019 ◽  
Vol 28 (6) ◽  
pp. 64-68
Author(s):  
Albina Vaičiulevičienė ◽  
Goda Jonaitytė ◽  
Vaida Batulevičienė
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Esherichia Coli

Užkrečiamųjų ligų ir AIDS centro (ULAC) teigimu, „Dezinfekcija yra viena iš užkrečiamųjų ligų perda­vimo rizikos mažinimo priemonių“ (2014). Jonizuotas rūgštinis vanduo pasirenkamas kaip mažiau toksiška žmgaus sveikatai ir aplinkai antimikrobinį poveikį tu­rinti medžiaga. Lietuvos UAB „Burbuliukas“ gamin­tojai siūlo alternatyvą cheminiams dezinfektantams - jonizuotą rūgštinį vandenį (anolitą), kuris saugus žmogui ir aplinkai bei pigesnis už cheminius dezin­fektantus ir naikina patogeninius mikroorganizmus. Tad šio darbo tikslas buvo įvertinti jonizuoto rūgšti­nio vandens antimikrobinį poveikį. Kauno kolegijos Medicinos fakulteto mikrobiologijos laboratorijoje UAB „Burbuliukas“ užsakymu buvo atliktas mikro­biologinis jonizuoto rūgštinio vandens (anolito) an­timikrobinis tyrimas plovinių metodu. Tirtas anolito poveikis užkrėstų paviršių (stalviršių, keramikinių plytelių, grindų linoleumo dangos) ir medicininių įrankių (stiklinių, keramikinių, metalinių) mikroor­ganizmais, turinčiais skirtingas biologines savybes. Tyrimui naudotos etaloninės mikroorganizmų kul­tūros, gautos OXOID Nr. 605068: Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Enterococcus faecalis ATCC 29212, Esherichia coli ATCC 25922, Klebsiella pneumo­niae ATCC 31488, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus subtilis ATCC6633, Candida albicans ATCC10231. Anolito ekspozicijos poveikis truko 15-30 min. Medicininiai instrumentai buvo pamerkti į anolitą, o paviršiai plau­nami anolite įmirkyta šluoste. Jonizuotas rūgštinis vanduo biocidiškai veikė skirtingas biologines savy­bes turinčius mikroorganizmus, kuriais buvo užkrėsti medicininiai instrumentai. Užkrėtus etaloninėmis mi­kroorganizmų kultūromis paviršius ir nuvalius anolitu du kartus su 15 minučių intervalu, po pirmo valymo aptiktos pavienės mikroorganizmų kolonijos, nuvalius antrą kartą - jonizuotas rūgštinis vanduo sunaikino sporines, nesporines bakterijas bei Candida grybus.

scholarly journals Influence of Two Root Canal Obturation Techniques with Resin Based Sealer to Enterococcus faecalis Penetration

2019 ◽  
Vol 2 (1) ◽  
pp. 21
Author(s):  
Aryadi Subrata ◽  
Anastasia Elsa Prahasti ◽  
Bernard Ongki Iskandar
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Root Canal Treatment ◽  
Root Canal Filling ◽  
Gutta Percha ◽  
Single Cone ◽  
Ah Plus ◽  
Bacterial Leakage ◽  
Warm Vertical Compaction

  Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment.   Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique.   Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis.   Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus).   Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.

scholarly journals SKIRTINGOSE LIETUVOS VIETOVĖSE SURINKTŲ ŽIEDADULKIŲ EKSTRAKTO ANTIMIKROBINIS VEIKIMAS

2021 ◽  
Vol 31 (7) ◽  
pp. 86-90
Author(s):  
Žaneta Maželienė ◽  
Gintarė Lingytė ◽  
Evelina Dailidaitė ◽  
Ingrida Viliušienė
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Listeria Monocytogenes ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Proteus Vulgaris ◽  
Microsoft Excel

Įvadas. Pasaulio sveikatos organizacijos (PSO) duomenimis, bakterijų atsparumas antibiotikams kelia didelę grėsmę visame pasaulyje ir sunkina infekcinių ligų gydymą [1]. Manoma, kad apie 70 proc. bakterijų, kurios sukelia infekcinius susirgimus ligoninėse, yra atsparios bent vienam iš gydymui naudojamų antibiotikų. Ši situacija skatina ieškoti kitų sprendimų, vienas iš jų – bičių produktus naudoti kaip natūralius antibiotikus dėl jų antimikrobinio veikimo. Tyrimo tikslas – nustatyti skirtingose Lietuvos vietovėse surinktų žiedadulkių ekstraktų antimikrobinį veikimą. Tyrimo metodika. Atliktas įvairiuose Lietuvos ūkiuose surinktų žiedadulkių skirtingos koncentracijos ekstraktų mikrobiologinis tyrimas. Nustatytas 50 žiedadulkių ekstraktų mėginių antimikrobinis poveikis dešimties etaloninių bakterijų kultūrų ir grybelio Candida albicans atžvilgiu. Tyrimo duomenų analizė atlikta naudojant aprašomąją statistiką, skaičiuojant dažnius bei grupių palyginimą pagal Kruskal Wallis, tyrimo duomenys apdoroti Microsoft Excel 2019 programa. Tyrimo rezultatai. Nustatyta, kad stipriausią antimikrobinį poveikį turėjo D žiedadulkių, surinktų Kaišiadorių rajone, ekstraktas. D ekstrakto antimikrobinis poveikis Staphylococcus epidermidis, Pseudomonas aeruginosa, Bacillus cereus, Listeria monocytogenes atžvilgiu nustatytas esant 0,025 ml ekstrakto 1 agaro ml. Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Proteus vulgaris ir grybelį Candida albicans, išskyrus Escherichia coli, ekstraktas veikė esant jo 0,0375 – 0,05 ml agaro 1 ml. Žiedadulkių ekstraktas gramteigiamąsias bakterijas veikia stipriau, lyginant su gramneigiamosiomis. Šis skirtumas siejamas su gramteigiamų ir gramneigiamų bakterijų ląstelės sienelės struktūros skirtumais. Išvados. Stipriausią antimikrobinį veikimą turi žiedadulkės, surinktos Kaišiadorių rajone. Nustatyta, kad žiedadulkių ekstraktai stipriau veikia gramteigiamąsias bakterijas, lyginant su gramneigiamosiomis.

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