In vitro culture of shoot apices from juvenile and adult yellow passion fruit plants

PRODUÇÃO DE MARACUJAZEIRO AMARELO NO SOLO COM CALCÁRIO E POTÁSSIO SOB IRRIGAÇÃO COM ÁGUA SALINA LOURIVAL FERREIRA CAVALCANTE1; CLODOALDO JÚNIOR OLIVEIRA SANTOS1; JOSÉ SIMPLÍCIO DE HOLANDA2; ANTONIO JOÃO DE LIMA NETO3; ANTÔNIO GUSTAVO DE LUNA SOUTO4 E TONY ANDRESON GUEDES DANTAS5 1 Universidade Federal da Paraíba, Departamento de Solos e Engenharia Rural, Rodovia BR 079 - Km 12, 58.397-000, Areia, PB, Brasil. E-mail: [email protected]; [email protected] 2 Empresa de Pesquisa Agropecuária do Rio Grande do Norte, Av. Eliza Branco Pereira dos Santos, s/nº, Parque das Nações, 59.158-160, Parnamirim, RN, Brasil. E-mail: [email protected] 3 Universidade Federal do Ceará, Departamento de Fitotecnia, Campus do Pici, Av. Mister Hull, 2977, Bloco 805, 60.356-001, Fortaleza, CE, Brasil. E-mail: [email protected] 4 Universidade Federal da Paraíba, Departamento de Fitotecnia e Ciências Ambientais, Rodovia BR 079 - Km 12, 58.397-000, Areia, PB, Brasil. E-mail: [email protected] 5 Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Rodovia CE-187, s/n, Aeroporto, 62.320-000, Tianguá, CE, Brasil. E-mail: [email protected] 1 RESUMO O experimento foi conduzido no município de Coronel Ezequiel, Rio Grande do Norte, para avaliar os efeitos do calcário calcítico e doses de K2O, na forma de cloreto de potássio, nos componentes de produção do maracujazeiro amarelo e no aumento da salinidade do solo provocado pela irrigação com água salina de 3,6 dS m-1 durante o período da aridez e na lixiviação dos sais do ambiente radicular das plantas promovida pelas águas do período chuvoso. Os tratamentos foram dispostos em delineamento em blocos casualizados usando arranjo fatorial 3 × 2, referente as doses de calcário de 1,4; 2,5 e 3,6 t ha-1, 80 e 160 kg ha-1 de K2O na forma de cloreto de potássio. Pelos resultados, a irrigação com água salina (3,6 dS m-1), em comparação com dados da literatura de plantas irrigadas com água de boa qualidade, não comprometeu a capacidade produtiva do maracujazeiro amarelo. Dentre os tratamentos, a combinação de 80 kg ha-1 de K2O com 3,6 t ha-1 de calcário calcítico proporcionou os maiores valores de massa média dos frutos, produção por planta e produtividade da cultura. Apesar da alta salinidade da água de irrigação elevar o caráter salino do solo no ambiente radicular das plantas durante o período da estiagem, as águas do período chuvoso e as condições físicas do solo proporcionam a lixiviação dos sais e possibilitam o uso de água com restrições salinas na agricultura. Palavras-chave: Passiflora edulis, calagem, lixiviação de sais CAVALCANTE, L. F.; SANTOS, C. J. O.; HOLANDA, J. S.; LIMA NETO, A. J.; SOUTO, A. G. L.; DANTAS, T. A. G. YELLOW PASSION FRUIT PLANTS PRODUCTION ON SOIL WITH LIME AND POTASSIUM UNDER IRRIGATION WITH SALINE WATER 2 ABSTRACT The experiment was carried out in Coronel Ezequiel county, Rio Grande do Norte, Brazil, in order to evaluate the effects of limestone and chloride potassium in production components of yellow passion fruit plants and soil salinity, caused by irrigation with saline water of 3.6 dS m-1 during the dry season, and salt leaching of the soil promoted by waters of the rainy season. Treatments were arranged in randomized blocks using factorial design of 3 × 2, referring to three levels of limestone, 1.4, 2.5 and 3.6 t ha-1 and two potassium levels, 80 and 160 kg ha-1 in potassium chloride form. According to present results, irrigation with saline water (3.6 dS m-1), in comparison with data from the literature about plants irrigated with non-saline water, did no compromise the productive capacity of yellow passion fruit. Among the treatments, the combination of 80 kg ha-1 of K2O with 3.6 t ha-1 of limestone provided the fruits production with more mean mass, yield per plant and crop yield. Although the high salinity of the irrigation water increases the soil saline character on root environment of the plants during the dry season, the rainy season waters and the soil physical conditions provide the salt leaching and allow the use of water with saline restrictions in agriculture. Keywords: Passiflora edulis, liming, salt lixiviation

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Fertilization With Nitrogen, Phosphorus and Potassium on Soil Fertility and Nutritional Status of Yellow Passion Fruit Plants

Journal of Agricultural Science ◽

10.5539/jas.v11n5p142 ◽

2019 ◽

Vol 11 (5) ◽

pp. 142

Author(s):

Rodrigo T. M. Miyake ◽

William H. S. Takata ◽

Nobuyoshi Narita ◽

José E. Creste

Keyword(s):

Nutritional Status ◽

Soil Fertility ◽

Nitrogen Fertilization ◽

Passion Fruit ◽

Triple Superphosphate ◽

Fruit Plants ◽

Phosphorus And Potassium ◽

Maximum Content ◽

Nitrogen Phosphorus ◽

Yellow Passion Fruit

The research had as objective to study the influence of the doses of nitrogen, phosphorus and potassium on soil fertility and nutritional status of yellow passion fruit plants. The experimental design was in randomized blocks in an incomplete fractioned factorial 1/2 (4 × 4 × 4), with four doses of N (150, 300, 600 and 1200 Kg-1 ha-1 yr-1 N); four doses of P2O5 (200, 400, 800 and 1600 kg-1 ha-1 yr-1) and four doses of K2O (100, 300, 500 and 700 kg-1 ha-1 yr-1). The fertilizers used were the ammonium nitrate = 32% N; triple superphosphate = 44% P2O5 and potassium chloride = 60% K2O. In the fertility of the nitrogen fertilization increased the content of phosphorus and sulfur. The doses of P2O5 increased the concentration of P in the soil. The doses of K2O influenced the increase in the content of K in the soil in the harvest 2013/2014. There was an interaction of the doses of N and K2O in the availability of K in the soil in the harvest 2012/13 with the maximum levels of K, in the doses of 200-400 kg N ha-1 and 600 to 700 kg ha-1 of K2O. In the nutritional state the yellow passion fruit plants was influenced by the doses of N for the sulfur content of the leaves. The maximum content of S leaf concentration of 3.63 g kg-1 was obtained in the dose estimated at 1120 kg ha-1 of P2O5.

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Modelling the in vitro pre-infection dynamics of Colletotrichum spp. isolated from yellow passion fruit in function of environmental variables

10.1101/781963 ◽

2019 ◽

Author(s):

Karinna Vieira Chiacchio Velame ◽

Hermes Peixoto Santos Filho ◽

Adelise de Almeida Lima ◽

Carlos Augusto Dórea Bragança ◽

Francisco Ferraz Laranjeira

Keyword(s):

Environmental Variables ◽

Climatic Conditions ◽

Passion Fruit ◽

Yield Reduction ◽

Post Harvest ◽

Infection Dynamics ◽

Mycelia Growth ◽

Wetness Period ◽

Yellow Passion Fruit

AbstractBrazil is the largest world producer of yellow passion fruit, but the mean yield (14.3t.ha-1) is less than half the potential of the crop. Part of this difference can be explained by plant health problems, including anthracnose caused by Colletotrichum spp. In regions with favorable climatic conditions, anthracnose can be a factor of significant yield reduction, but these regions have not yet been zoned. The objective of this study was to model the pre-infection dynamics of the fungus. The influence of temperature and photoperiod was studied on mycelia growth, sporulation and conidia germination. Mathematical models were fitted to the results and the optima for the environmental variables were estimated. The maximum mycelia growth was estimated to occur at 26.5°C. Between 24.5°C and 28.5°C the fungus grew from 95% to 100% of the estimated maximum. Temperatures below 13°C or above 34°C were harmful to mycelia growth. Temperatures over 26°C were the most favorable to sporulation while below 13°C sporulation was only 5% of the maximum. Optimum germination occurred between 25°C and 29°C with the ideal wetness period between 11h and 13h. These results can be used as a basis for zoning the risk of anthracnose occurrence in passion fruit producing regions.Significance and Impact of the StudyMany diseases affect the yellow passion fruit crop, limiting its yield; among them anthracnose, caused by Colletotrichum spp. The disease occurs in both field (leaf and stem symptoms) and post-harvest (fruits) conditions. Understanding the role environmental conditions play in the biological cycle of such diseases is essential for developing management strategies. By modelling mycelial growth, spore production and spore germination of Colletotrichum spp. as affected by temperature, photoperiod and wetness period, it was possible to characterize the pathogen’s pre-infectional dynamics. The results should be used as a first approximation to estimate the risk of anthracnose occurrence in pre- or post-harvest.

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Detection of a resistance gradient to Passion fruit woodiness virus and selection of ‘yellow’ passion fruit plants under field conditions

Genetics and Molecular Research ◽

10.4238/vol7-4gmr484 ◽

2008 ◽

Vol 7 (4) ◽

pp. 1209-1216 ◽

Cited By ~ 23

Author(s):

C.B.M. Cerqueira-Silva ◽

C.N. Moreira ◽

A.R. Figueira ◽

R.X. Correa ◽

A.C. Oliveira

Keyword(s):

Passion Fruit ◽

Field Conditions ◽

Fruit Plants ◽

Passion Fruit Woodiness Virus ◽

Yellow Passion Fruit ◽

Selection Of

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Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit

Acta Scientiarum Biological Sciences ◽

10.4025/actascibiolsci.v42i1.48321 ◽

2020 ◽

Vol 42 ◽

pp. e48321

Author(s):

João Arthur dos Santos Oliveira ◽

Andressa Domingos Polli ◽

Julio Cesar Polonio ◽

Ravely Casarotti Orlandelli ◽

Hélio Conte ◽

...

Keyword(s):

Endophytic Fungi ◽

Growth Promotion ◽

Passion Fruit ◽

Agricultural Field ◽

Bean Plants ◽

Causative Agents ◽

Colonization Frequency ◽

Greenhouse Assay ◽

Yellow Passion Fruit

Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried out greenhouse experiments in bean seedlings. A high colonization frequency was obtained (89%), and the molecular identification based on DNA sequencing attested Colletotrichum as the most frequent genus and minor occurrence of Curvularia endophytes. The endophytes tested showed different types of competitive interactions in in vitro antagonism inhibition rate ranging from 28.8 to 48.8%. There were 10 promising antagonists tested for their antagonist activity of crude extracts of secondary metabolites, in which strain PE-36 (20.8%) stood out among the other strains evaluated. In the greenhouse assay, plants inoculated only with endophyte Colletotrichum sp. PE-36 was symptomless and suggest that the endophyte strengthened the growth promotion in common bean plants, especially in the root length and number of leaves when compared to control plants and other treatments. Despite many fungi of Colletotrichum genus being described as causative agents of anthracnose, in this study, the plant sampled was colonized predominantly by Colletotrichum endophytes living in asymptomatic relationship. By the way, we come across a Colletotrichum sp. endophyte able to antagonize a Colletotrichum sp. pathogen.

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Brassinosteroid analogue effects on the yield of yellow passion fruit plants (Passiflora edulis f. flavicarpa)

Scientia Horticulturae ◽

10.1016/j.scienta.2006.06.030 ◽

2006 ◽

Vol 110 (3) ◽

pp. 235-240 ◽

Cited By ~ 22

Author(s):

Mara de Menezes Assis Gomes ◽

Eliemar Campostrini ◽

Nilton Rocha Leal ◽

Alexandre Pio Viana ◽

Tiago Massi Ferraz ◽

...

Keyword(s):

Passion Fruit ◽

Passiflora Edulis ◽

Fruit Plants ◽

Yellow Passion Fruit

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Resistance to Passion fruit woodiness virus in Transgenic Passionflower Expressing the Virus Coat Protein Gene

Plant Disease ◽

10.1094/pd-90-1026 ◽

2006 ◽

Vol 90 (8) ◽

pp. 1026-1030 ◽

Cited By ~ 33

Author(s):

F. Trevisan ◽

B. M. J. Mendes ◽

S. C. Maciel ◽

M. L. C. Vieira ◽

L. M. M. Meletti ◽

...

Keyword(s):

Coat Protein ◽

Transgenic Plants ◽

In Vitro Selection ◽

Passion Fruit ◽

Cp Gene ◽

Virus Coat ◽

Passion Fruit Woodiness Virus ◽

Leaf Disks ◽

Yellow Passion Fruit

We report the use of the coat protein (CP) gene from Passion fruit woodiness virus (PWV) to produce resistant transgenic plants of yellow passion fruit. A full-length CP gene from a severe PWV isolate from the state of São Paulo, Brazil (PWV-SP) was cloned into pCAMBIA 2300 binary vector, which was further introduced into Agrobacterium tumefaciens strain EHA 105. Leaf disks were used as explants for transformation assays, e.g., 2,700 and 2,730 disks excised from plants from the Brazilian cultivars IAC-275 and IAC-277, respectively. In vitro selection was performed in kanamycin. After transferring to the elongation medium, 119 and 109 plantlets of IAC-275 and IAC-277, respectively, were recovered. Integration of the PWV CP gene was confirmed in seven of eight plants evaluated by Southern blot analysis, showing different numbers of insertional events for the CP gene. Three transgenic plants (T3, T4, and T7) expressed the expected transcript, but the 32 kDa PWV CP was detected by Western blot in only two plants (T3 and T4). The results of three successive mechanical inoculations against the transgenic plants using three PWV isolates showed that the primary transformant T2 of IAC-277 was immune to all isolates.

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