scholarly journals Preliminary trials of Genotype-by-Environment Interactions for Sugars, Organic Acids, and Amino Acids of Table Grape Varieties in Japan

10.5109/24141 ◽  
1997 ◽  
Vol 41 (3/4) ◽  
pp. 157-163
Author(s):  
Mikio Shiraishi ◽  
Shin-ichi Shiraishi
Author(s):  
O. K. Vlasova ◽  
Z. K. Bakhmulaeva ◽  
T. I. Daudova ◽  
S. A. Magadova

The paper presents the results of a study on the content of free amino acids, including essential ones, vitamins C and P, biologically important macro- and microelements in table grapes of the middle ripening varieties Ag raisins, Vezne, Galan, Hamburg Muscat and Samur, cultivated on the plain in Southern Dagestan Region. The qualitative composition and quantitative content of free amino acids were determined by HPLC on an AAA-881 analyzer. Amino acids tryptophan, cystine and methionine were identified by paper chromatography due to the fact that they decompose when exposed to a temperature of 102 ° C in the amino analyzer reactor. The mass concentration of vitamin C was identified titrimetrically, vitamin P was identified colorimetrically (FEK-56M, Russia), the mineral composition of grapes was identified by flame and atomic absorption photometry using Flapho-4 (Germany) and Hitachi-208 (Japan) devices. Each of the varieties had 18 amino acids, including a full set of essential ones. The total number of detected amino acids was 368.5 (Ag raisins) - 279.9 mg / dm3 (Vezne), and irreplaceable ones - from 103.8 (Hamburg muscat) to 132.4 mg / dm3 (Ag raisins). All experimental grape samples contained vitamins C, from 2.6 (Galan) to 3.7 mg / dm3 (Ag raisins) and P - from 78.0 (Samur) to 131.3 mg / dm3 (Galan), as well as physiologically important minerals. Studies have shown that in terms of the amount of free amino acids, including essential vitamins C and P, healthy minerals, such grapes as Ag raisins, Vezne, Galan, Hamburg and Samur Muscat, grown in the south of Dagestan are natural products with functional properties. Eating it fresh contributes to the normalization of metabolic processes in the human body. Additionally, these grapes can be successfully used as a raw material for the production of multicomponent functional food.


Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 248
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Ye Jin Kim ◽  
Bao Van Nguyen ◽  
Ye Eun Park ◽  
...  

This study aimed to elucidate the variations in primary and secondary metabolites during Lycorisradiata flower development using high performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). The result showed that seven carotenoids, seven phenolic acids, three anthocyanins, and galantamine were identified in the L. radiata flowers. Most secondary metabolite levels gradually decreased according to the flower developmental stages. A total of 51 metabolites, including amines, sugars, sugar intermediates, sugar alcohols, amino acids, organic acids, phenolic acids, and tricarboxylic acid (TCA) cycle intermediates, were identified and quantified using GC-TOFMS. Among the hydrophilic compounds, most amino acids increased during flower development; in contrast, TCA cycle intermediates and sugars decreased. In particular, glutamine, asparagine, glutamic acid, and aspartic acid, which represent the main inter- and intracellular nitrogen carriers, were positively correlated with the other amino acids and were negatively correlated with the TCA cycle intermediates. Furthermore, quantitation data of the 51 hydrophilic compounds were subjected to partial least-squares discriminant analyses (PLS-DA) to assess significant differences in the metabolites of L. radiata flowers from stages 1 to 4. Therefore, this study will serve as the foundation for a biochemical approach to understand both primary and secondary metabolism in L. radiata flower development.


Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1377
Author(s):  
Song-Hui Soung ◽  
Sunmin Lee ◽  
Seung-Hwa Lee ◽  
Hae-Jin Kim ◽  
Na-Rae Lee ◽  
...  

Numerous varieties of doenjang are manufactured by many food companies using different ingredients and fermentation processes, and thus, the qualities such as taste and flavor are very different. Therefore, in this study, we compared many products, specifically, 19 traditional doenjang (TD) and 17 industrial doenjang (ID). Subsequently, we performed non-targeted metabolite profiling, and multivariate statistical analysis to discover distinct metabolites in two types of doenjang. Amino acids, organic acids, isoflavone aglycones, non-DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4- one) soyasaponins, hydroxyisoflavones, and biogenic amines were relatively abundant in TD. On the contrary, contents of dipeptides, lysophospholipids, isoflavone glucosides and DDMP-conjugated soyasaponin, precursors of the above-mentioned metabolites, were comparatively higher in ID. We also observed relatively higher antioxidant, protease, and β-glucosidase activities in TD. Our results may provide valuable information on doenjang to consumers and manufacturers, which can be used while selecting and developing new products.


1988 ◽  
Vol 15 (4) ◽  
pp. 557 ◽  
Author(s):  
MJ Canny ◽  
ME Mccully

Three methods of sampling xylem sap of maize roots were compared: sap bleeding from the stem cut just above the ground; sap bleeding from the cut tops of roots still undisturbed in the ground; and sap aspirated from excavated roots under reduced pressure. The bleeding saps were often unobtainable. When their composition was measured with time from cutting, the concentrations of the major solutes approximately doubled in 2 h. Aspirated sap was chosen as the most reliable sample of root xylem contents. Solute concentrations of the saps showed great variability between individual roots for all solutes, but on average the concentrations found (in �mol g-1 sap) were: total amino acids, 1.8; nitrate, 1.8; sugars (mainly sucrose), 5.4; total organic acids, 18.3. Individual amino acids also varied greatly between roots. Glutamine, aspartic acid and serine were generally most abundant. The principal organic acid found was malic, approximately 8 �mol g-1. From these analyses the ratios of carbon in the fractions (sugars : amino acids : organic acids) = (44 : 6 : 50). 14Carbon pulse fed to a leaf appeared in the root sap within 30 min, rose to a peak at 4-6 h, and declined slowly over a week. During all this time the neutral, cation and anion fractions were sensibly constant in the proportions 86 : 10 : 4. The 14C therefore did not move towards the equilibrium of 12C-compounds in the sap. It is argued that the results do not support a hypothesis of formation of amino carbon from recent assimilate and reduced nitrate in the roots and an export of this to the shoot in the transpiration stream.


Euphytica ◽  
2017 ◽  
Vol 213 (5) ◽  
Author(s):  
Megan M. Mathey ◽  
Sonali Mookerjee ◽  
Lise L. Mahoney ◽  
Kazim Gündüz ◽  
Umesh Rosyara ◽  
...  

2020 ◽  
Author(s):  
Edwin Lauer ◽  
Andrew Sims ◽  
Steven McKeand ◽  
Fikret Isik

Abstract Genetic parameters were estimated using a five-series multienvironment trial of Pinus taeda L. in the southern USA. There were 324 half-sib families planted in five test series across 37 locations. A set of six variance/covariance matrices for the genotype-by-environment (G × E) effect for tree height and diameter were compared on the basis of model fit. In single-series analysis, extended factor analytical models provided generally superior model fit to simpler models for both traits; however, in the combined-series analysis, diameter was optimally modeled using simpler variance/covariance structures. A three-way compound term for modeling G × E interactions among and within series yielded substantial improvements in terms of model fit and standard errors of predictions. Heritability of family means ranged between 0.63 and 0.90 for both height and diameter. Average additive genetic correlations among sites were 0.70 and 0.61 for height and diameter, respectively, suggesting the presence of some G × E interaction. Pairs of sites with the lowest additive genetic correlations were located at opposite ends of the latitude range. Latent factor regression revealed a small number of parents with large factor scores that changed ranks significantly between southern and northern environments. Study Implications Multienvironmental progeny tests of loblolly pine (Pinus taeda L.) were established over 10 years in the southern United States to understand the genetic variation for the traits of economic importance. There was substantial genetic variation between open-pollinated families, suggesting that family selection would be efficient in the breeding program. Genotype-by-environment interactions were negligible among sites in the deployment region but became larger between sites at the extremes of the distribution. The data from these trials are invaluable in informing the breeding program about the genetic merit of selection candidates and their potential interaction with the environment. These results can be used to guide deployment decisions in the southern USA, helping landowners match germplasm with geography to achieve optimal financial returns and conservation outcomes.


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