scholarly journals Molecular Prevalence of Mycoplasma capri in Thanjavur region

2020 ◽  
Vol 1 (1) ◽  
pp. 38-43
Author(s):  
Manimaran K. ◽  
Balakrishnan S. ◽  
Sangeetha A. ◽  
Dhanalakshmi, M.K. ◽  
Sivakumar T.
Keyword(s):  
Rural Areas ◽  
Tamil Nadu ◽  
Respiratory Diseases ◽  
Small Ruminants ◽  
Mycoplasma Infection ◽  
Pcr Assay ◽  
Specific Primers ◽  
The Past ◽  
Goat Population ◽  
Cauvery Delta

Goat rearing has emerged as a significant resource in rural areas even under unfavorable environmental conditions. Goat population in India has grown over the past few decades and stands at 148.88 million during 2019 (Census, 2019) owing to their greater socio-economic relevance. Goats, while being generally resistant to diseases, are highly susceptible to respiratory diseases, which account for almost 50% mortality amongst them. Irrespective of the etiology, the infectious respiratory diseases of sheep and goats contribute to 5.6% of the total diseases of small ruminants and is responsible for around 28.7 % mortality. Pneumonia has been noticed as one of the most frequently encountered condition and is responsible for around 28.7% mortality. Amongst various infections, Mycoplasmosis is one of the most dreaded diseases of goats. The present study was undertaken to detect the Mycoplasma capri infection in cauvery delta region of Tamil Nadu. All the collected tissue materials were subjected to isolation and PCR assay with Mycoplasma group specific primers (GPO- 1 and MGSO) which yielded 715 bp product and Mycoplasma capri specific primers (P 4 and P 6) which gave an amplicons of 195 bp products. The findings indicate that the PCR assay is very simple and useful method for detecting the mycoplasma infection directly from the tissue materials in a very short span. Keywords: Mycoplasma capri, PCR, Infection, detection and tissue materials

scholarly journals Migratory Sheep Farming Practices in Cauvery Delta Zone in Tamil Nadu

2020 ◽  
Vol 16 (01) ◽  
pp. 62-65
Author(s):  
Mahesh Chander ◽  
MS Kannadhasan ◽  
D Bardhan
Keyword(s):  
Prevention And Control ◽  
Tamil Nadu ◽  
Disease Risk ◽  
Small Ruminants ◽  
Structured Interview ◽  
Farming Practices ◽  
Interview Schedule ◽  
Ex Post ◽  
Cauvery Delta ◽  
And Control

Migratory sheep, due to its movement, poses threat of spreading diseases among small ruminants. Considering this, the study was carried out to identify the focus area of extension intervention among the migratory sheep farmers to disseminate scientifically recommended practices which minimize disease risk and enhance flock health. The study was conducted following ex post fact research design at Venganur village, Cuddalore district, Tamil Nadu. Using a semi-structured interview schedule, data were personally collected from 30 migratory sheep farmers who were selected purposively. The findings were subjected to focussed group interview involving seven farmers. The results envisaged a wider difference among the farmers from 100 per cent non-adoption of balanced ration to 100.00 per cent adoption of deworming. Therefore, extension interventions need to be intensified to educate the farmers on technologies which help in disease prevention and control.

scholarly journals Contagious caprine pleuropneumonia and its current picture in Pakistan: a review

2013 ◽  
Vol 58 (No. 8) ◽  
pp. 389-398 ◽  
Author(s):  
S. Samiullah
Keyword(s):  
Pleural Fluid ◽  
Causative Agent ◽  
Respiratory Diseases ◽  
Small Ruminants ◽  
Mycoplasma Capricolum ◽  
Contagious Caprine Pleuropneumonia ◽  
Goat Population ◽  
Mycoplasma Mycoides ◽  
Cluster A

Contagious caprine pleuropneumonia (CCPP) is caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) which belongs to the Mycoplasma mycoides cluster, a group of five closely related Mycoplasmas, pathogenic to ruminants. The true lesions of CCPP are restricted to the alveolar tissues of infected goats, which distinguish it from other respiratory diseases of small ruminants caused by members of the Mycoplasma mycoides cluster. The typical signs of CCPP are an accumulation of pleural fluid, unilateral hepatisation, adhesions, pleurisy and pleuropneumonia which clearly differentiate it from “MAKePS” syndrome caused by Mycoplasma mycoides subsp. capri (Mmc). The available literature on CCPP shows that so far in Pakistan, the true causative agent (Mccp) of this disease has only been isolated in the Pashin District of Balochistan and that the disease is more frequently confused with other respiratory diseases of goat caused by the Mycoplasma mycoides cluster. The lack of suitable techniques and extensive knowledge in the field is a big limitation for the isolation and characterisation of Mccp from prevailing CCPP-like cases in the goat population of Pakistan.  

Rapid detection of infection due to Mycoplasma mycoides subsp. capri in experimental goats by PCR by assay

2017 ◽  
Author(s):  
K. Manimaran ◽  
V. P. Singh
Keyword(s):  
Rapid Detection ◽  
Cross Reactivity ◽  
Mycoplasma Infection ◽  
Pcr Assay ◽  
Isolation And Identification ◽  
Specific Primers ◽  
Poor Growth ◽  
Mycoplasma Mycoides ◽  
Causative Agents ◽  
Important Disease

Mycoplasma causes a number of important disease viz., CBPP, CCPP, CRD, Arthritis, Contagious agalactiae and Mastitis in livestock and poultry. The conventional method of isolation and identification of the causative agents of these diseases are generally employed in conjunction with the serology which have the limitation of cross reactivity with other mycoplasma and poor growth of the organisms. To overcome these problems, the PCR assay which is rapid, sensitive and specific was used in the present study to detect the mycoplasma infection due to M.Mycoides subsp.capri directly from the tissue materials viz., lungs, lymphnodes, spleen and liver from the experimentally infected goats. All the tissue materials were subjected to PCR assay with cluster specific primers (MC 323 and MC 358), which yielded approximately 1.5 kbp long product. Further,. It was characterized by using mycoides group specific primers (MM 450 and MM 451) and Mycoplasma capri specific primers (P 4 and P 6) which gave an amplicons of 574 bp and 195 bp products, respectively. The findings indicate that the PCR assay is very simple and useful method for detecting the mycoplasma infection directly from the tissue materials in a very short span.

A Hemi-nested, Allele Specific, Whole Blood PCR Assay for the Detection of the Factor V Leiden Mutation

1997 ◽  
Vol 77 (06) ◽  
pp. 1154-1155 ◽  
Author(s):  
Gary D Sinclair ◽  
Sandra Low ◽  
Man-Chiu Poon
Keyword(s):  
Whole Blood ◽  
Protein C ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Restriction Enzyme Digestion ◽  
Factor V ◽  
Pcr Assay ◽  
Specific Primers ◽  
Factor V Leiden Mutation ◽  
Allele Specific

SummaryWe describe a novel hemi-nested, allele specific whole blood PCR assay for detection of the factor V Leiden mutation associated with the plasma defect, activated protein C resistance. This assay utilizes 5 μl of whole blood without prior DNA extraction. The hemi-nested design, employing an outer primer pair in combination with nested, allele specific primers obviates the need for restriction enzyme digestion. PCR reactions are analysed directly on agarose or polyacrylamide minigels. The assay confirmed the genotypes of 50 individuals previously categorized by PCR and Mnll digestion, and has been subsequently utilized in the genotyping of 445 individuals referred for thrombosis studies.

Buddhism in Crisis? Institutional Decline in Modern Japan

2012 ◽  
Vol 28 (2) ◽  
pp. 233-263 ◽  
Author(s):  
Ian Reader
Keyword(s):  
Rural Areas ◽  
Japanese Population ◽  
Public Perceptions ◽  
Japanese People ◽  
Death Rituals ◽  
Modern Times ◽  
The Past ◽  
Modern Cities ◽  
Modern Japan ◽  
Institutional Decline

Concerns that established temple Buddhism in Japan is in a state of crisis have been voiced by priests in various sectarian organizations in recent years. This article shows that there is a very real crisis facing Buddhism in modern Japan, with temples closing because of a lack of support and of priests to run them, and with a general turn away from Buddhism among the Japanese population. In rural areas falling populations have led to many temple closures, while in the modern cities people are increasingly turning away from the prime area in which Japanese people have traditionally engaged with Buddhist temples — the processes of death and their aftermath. Partly this is due to competition from new secular funeral industries, but partly also it is because public perceptions of Buddhism — which has become over-reliant on death rituals in Japan — have become highly negative in modern times. Even practices which have often been seen as areas in which Buddhist temples have been able to attract people — such as pilgrimages — are proving less successful than in the past, contributing further to a sense of crisis that threatens to undermine Buddhism’s roots in Japan.

Исследование психологических особенностей восприятия города Бишкек городскими и сельскими студентами

2019 ◽  
pp. 98-100
Author(s):  
Д. М. Кудайбердиева
Keyword(s):  
Urban Environment ◽  
Rural Areas ◽  
Space Perception ◽  
Language Barrier ◽  
Rural Students ◽  
Adaptation Period ◽  
Educational Work ◽  
The Past ◽  
Spatial Environment ◽  
The City

Аннотация. В данной статье рассматривается проблема восприятия городской среды студентами из города и сельской местности. Обозначены определения терминов «среда», «пространственная среда», «городская среда». Проведено экспериментальное сравнительное исследование особенностей восприятия города Бишкек городскими и сельскими студентами в количестве 50 человек. Статья содержит результаты проведенного анализа исследования. Выявлены различия и сходства в восприятии города Бишкек у студентов двух групп. В контексте восприятия города изучена неудовлетворенность прошлым и настоящим временем, трудности языкового барьера у студентов из сельской местности. Также было выявлено, что актуальной для студентов обеих групп является потребность в социальных контактах, общении, включенности в социум. Полученные результаты будут иметь пользу в проведении воспитательной работы со студентами из сельской местности, в оказании поддержки им в период адаптации с учетом особенностей восприятия города студентами. Ключевые слова. среда, городская среда, восприятие пространства, городские студенты, сельские студенты, восприятие города Бишкек, неудовлетворенность прошлым и настоящим, языковой барьер, учеба в городе, сходства и различия в восприятии города. Аннотация. Бул макалада шаардык жана айылдан келген студенттердин шаардык чөйрөнү кабыл алуу көйгөйү каралган. «Чөйрө», «мейкиндик чөйрө», «шаардык чөйрө» терминдеринин түшүнүктөрү келтирилген. 50 адамдан турган шаардык жана айылдык студенттердин Бишкек шаарын кабыл алуу өзгөчөлүктөрүнө эксперименталдык салыштырма изилдөө жүргүзүлгөн. Макалада изилдөөнүн анализ жыйынтыктары камтылган. Эки студенттик топторунун Бишкек шаарын кабыл алуусунун айырмачылыктары жана окшоштуктары белгиленген. Шаарды кабыл алуу контекстинде өткөн жана учур чактарга канагаттанбоо, тил барьеринин кыйынчылыктары изилденди. Ошондой эле студенттердин эки тобуна актуалдуу болуп социалдык катнаштарга, баарлашууга, коомго кошулуу муктаждыгы белгиленүүдө. Алынган жыйынтыктар студенттердин шаарды кабыл алуу өзгөчөлүктөрүнө эске алып, айылдан келген студенттерге адаптация убагында колдоо көрсөтүү максатында тарбиялык иштерди жүргүзүүдө жардам берет. Түйүндүү сөздөр. Чөйрө, шаардык чөйрө, мейкиндикти кабыл алуу, шаардык студенттер, айылдык студенттер, Бишкек шаарын кабыл алуу, өткөн жана учур чакка канагаттанбоо, тил барьери, шаарда окуу, шаарды кабыл алуусунун айырмачылыктары жана окшоштуктары. Annotation. This article addresses the problem of perception of the urban environment by students from the city and countryside. The definitions of the terms “environment”, “spatial environment”, and “urban environment” are indicated. An experimental comparative study of the characteristics of the perception of the city of Bishkek by urban and rural students in the amount of 50 people was conducted. The article contains the results of the analysis of the study. The differences and similarities in the perception of the city of Bishkek among students of the two groups are revealed. In the context of the perception of the city, dissatisfaction with the past and the present has been studied, and the difficulties of the language barrier among students from rural areas. It was also revealed that the need for social contacts, communication, inclusion in society is relevant for students of both groups. The results will have the benefit of conducting educational work with students from rural areas, in providing support to them during the adaptation period, taking into account the peculiarities of the perception of the city by students. Кeywords. Environment, urban environment, space perception, urban students, rural students, perception of the city of Bishkek, dissatisfaction with the past and the present, language barrier, studying in the city, similarities and differences in the perception of the city.

Evolving appropriate zinc fertilization strategy for rice-rice (Oryza sativa) cropping system in Cauvery Delta Zone

2019 ◽  
Vol 56 (3) ◽  
pp. 294-304
Author(s):  
C Sharmila Rahale
Keyword(s):  
Oryza Sativa ◽  
Tamil Nadu ◽  
Field Experiments ◽  
Foliar Spray ◽  
Cropping System ◽  
Green Leaf ◽  
Farm Yard Manure ◽  
Zinc Fertilization ◽  
Initiation Stage ◽  
Cauvery Delta

Six field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai to evolve suitable zinc fertilization method for rice - rice (Oryza sativa L.) cropping system in Cauvery delta zone. The treatment includes: T1 : Control, T2 : 100 g zinc sulphate (ZnSO4)/cent in nursery alone., T3 : root dipping alone in 2 % zinc oxide (ZnO) solution, T4 : 25 kg ZnSO4 ha-1, T5 : 37.5 kg ZnSO4 ha-1, T6 : 25 kg ZnSO4 ha-1+ Farm Yard Manure (FYM) 12.5 t ha-1, T7 : 25 kg ZnSO4 ha-1 + Green Leaf Manure (GLM) 6.5 t ha-1, T8 : Tamil Nadu Agricultural University Micro Nutrient (TNAU MN) mixture 25 kg ha-1 as Enriched Farm Yard Manure (EFYM), T9 : TNAU MN mixture 37.5 kg ha-1 as EFYM, T10 : Foliar spray of 0.5% ZnSO4 + 1 % urea at tillering and panicle initiation stage, T11 : 100g ZnSO4 /cent in nursery alone + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T2+ T10), T12 : root dipping alone in 2 % ZnO solution + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T3 + T10), T13: 100 g ZnSO4 /cent in nursery alone + root dipping alone in 2 % ZnO solution + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T2 + T3 + T10). The treatments T5, T6, T7 and T9 were skipped in rabi season to know the residual effect of these treatments in the subsequent season. Among the treatment combinations, application of 25 kg ZnSO4 ha-1 + FYM 12.5 t ha-1 recorded higher grain yield in both kharif (6232 kg ha-1) and rabi (6236 kg ha-1) seasons. The same treatment combination recorded higher Zn content and Zn uptake as well. Regarding soil nutrient content, the same treatment recorded higher N, P and K content. This treatment was followed by application of 25 kg ZnSO4 ha-1 + green leaf manure 6.5 t ha-1. The experimental findings suggested that combination of organic and inorganic sources not only increased the yield but also improves soil health in Cauvery delta zone.

AFLP-derived, Codominant Markers for Locus-specific Applications

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 514e-514
Author(s):  
James M. Bradeen ◽  
Philipp W. Simon
Keyword(s):  
Linkage Mapping ◽  
Large Scale ◽  
Pcr Primers ◽  
Inverse Pcr ◽  
Sequence Information ◽  
Pcr Assay ◽  
Specific Primers ◽  
Simultaneous Evaluation ◽  
Feral Populations ◽  
Diversity Assessment

The amplified fragment length polymorphism (AFLP) is a powerful marker, allowing rapid and simultaneous evaluation of multiple potentially polymorphic sites. Although well-adapted to linkage mapping and diversity assessment, AFLPs are primarily dominant in nature. Dominance, relatively high cost, and technological difficulty limit use of AFLPs for marker-aided selection and other locus-specific applications. In carrot the Y2 locus conditions carotene accumulation in the root xylem. We identified AFLP fragments linked to the dominant Y2 allele and pursued conversion of those fragments to codominant, PCR-based forms useful for locus-specific applications. The short length of AFLPs (≈60 to 500 bp) precludes development of longer, more specific primers as in SCAR development. Instead, using sequence information from cloned AFLP fragments for primer design, regions outside of the original fragment were amplified by inverse PCR or ligation-mediated PCR, cloned, and sequenced. Differences in sequences associated with Y2 vs. y2 allowed development of simple PCR assays differentiating those alleles. PCR primers flanking an insertion associated with the recessive allele amplified differently sized products for the two Y2 alleles in one assay. This assay is rapid, technologically simple (requiring no radioactivity and little advanced training or equipment), reliable, inexpensive, and codominant. Our PCR assay has a variety of large scale, locus-specific applications including genotyping diverse carrot cultivars and wild and feral populations. Efforts are underway to improve upon conversion technology and to more extensively test the techniques we have developed.

Impact of Elected Women Representatives of Panchayati Raj in Physical and Financial and Business Assets Development of Rural Women: A Study among the Rural Women’s of Tiruvannamalai District, Tamil Nadu[1]

2017 ◽  
Vol 4 (24) ◽  
Author(s):  
Kalaichelvi Sivaraman ◽  
Rengasamy Stalin
Keyword(s):  
Rural Women ◽  
Rural Areas ◽  
Urban Areas ◽  
Direct Democracy ◽  
Tamil Nadu ◽  
Local Level ◽  
Rural And Urban Areas ◽  
The Government ◽  
The Impact ◽  
Panchayati Raj

This research paper is the part of Research Project entitled “Impact of Elected Women Representatives in the Life and Livelihood of the Women in Rural Areas: With Special Reference to Tiruvannamalai District, Tamil Nadu” funded by University of Madras under UGC-UPE Scheme.The 73rd and 74th amendments of the Constitution of India were made by the government to strengthen the position of women and to create a local-level legal foundation for direct democracy for women in both rural and urban areas. The representation for women in local bodies through reservation policies amendment in Constitution of India has stimulated the political participation of women in rural areas. However, when it’s comes to the argument of whether the women reservation in Panchayati Raj helps or benefits to the life and livelihood development of women as a group? The answer is hypothetical because the studies related to the impact of women representatives of Panchayati Raj in the life and livelihood development of women was very less. Therefore, to fill the gap in existing literature, the present study was conducted among the rural women of Tiruvannamalai district to assess the impact of elected women representatives in the physical and financial and business development of the women in rural areas. The findings revealed that during the last five years because of the women representation in their village Panjayati Raj, the Physical Asset of the rural women were increased or developed moderately (55.8%) and Highly (23.4%) and the Financial and Business Asset of the rural women were increased or developed moderately (60.4%) and Highly (18.7%).

Traditional and Customary Law: Adat

2018 ◽  
Author(s):  
Simon Butt ◽  
Tim Lindsey
Keyword(s):  
Judicial Review ◽  
Rural Areas ◽  
Small Proportion ◽  
Local Governments ◽  
The State ◽  
Customary Law ◽  
Constitutional Court ◽  
The Past ◽  
Statutory Protection ◽  
Traditional Communities

Many Indonesians—primarily those living in rural areas—still follow customary law (adat). The precise rules and processes of that adat differ significantly from place to place, even within short distances. This chapter shows that for many decades, adat has been subservient to national law. State-made law overrode it, leaving it applicable only in a very small proportion of cases where no national law applied, where judges could apply it as ‘living law’. Even in these cases, many judges ignored adat or distorted it when deciding cases. The 1945 Constitution was amended in 2000 to require the state to formally recognize and respect customary law, as practised in traditional communities. The Constitutional Court has given effect to this in various judicial review cases, as have some statutes enacted in the past decade or so. However, this constitutional and statutory ‘protection’ has been impeded in practice by requirements for traditional communities to be formally ‘recognized’ by their local governments, many of whom have been unresponsive to calls for recognition.

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