Different effectiveness of Helicobacter pylori lipopolysaccharides with or without LewisXY determinants in stimulating the secretion of proinflammatory cytokines IL-8 and TNF-α by peripheral blood mononuclear leukocytes

ABSTRACT The effects of moxifloxacin, a new methoxyfluoroquinolone, on the production of proinflammatory cytokines from human peripheral blood mononuclear cells (PBMCs) were evaluated. Moxifloxacin inhibited the production of tumor necrosis factor alpha (TNF-α) and/or interleukin-6 (IL-6) by PBMCs stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), and heat-killed bacteria in a concentration-dependent manner without cytotoxic effects. The addition of moxifloxacin reduced the population of cells positive for CD-14 and TNF-α and for CD-14 and IL-6 among the LPS- or LTA-stimulated PBMCs. By Western blot analysis, moxifloxacin pretreatment reduced the degradation of IκBα in LPS-stimulated PBMCs. In conclusion, moxifloxacin could interfere with NF-κB activation by inhibiting the degradation of IκBα and reduce the levels of production of proinflammatory cytokines.

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ID: 1030 Observing an expression of TNF-α, IL10 and HBEGF on peripheral blood mononuclear cells (CD14+) of a guttate psoriatic patient at Ho Chi Minh City

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.306 ◽

2017 ◽

Vol 4 (S) ◽

pp. 105

Author(s):

Nguyen Hong Viet ◽

Pham Cuc Hoa

Keyword(s):

Peripheral Blood ◽

Mononuclear Cells ◽

Psoriatic Patient ◽

Mononuclear Leukocytes ◽

Chromosome 2 ◽

Heparin Binding ◽

Peripheral Blood Mononuclear ◽

Streptococcus Pneumonia ◽

Tnf Α ◽

Guttate Psoriasis

Psoriasis is a chronic autoimmune disease in dermatology. The experiment collected peripheral blood samples from cohorts. These samples were treated and sorted mononuclear leukocytes with LPS receptors or CD14. Then, they were stimulated by a lysis of Streptococcus pneumonia in 24 hours. These cells were harvested in 0, 1, 24, 48, 72,120 (day 5) and 168 (day 7) hours and were isolated total mRNA. All mRNA samples were converted into the cDNA samples which were utilised to indirectly evaluate the cytokine expression of TNF-α, IL10 and Heparin-binding EGF-like growth factor (HBEGF) on the mononuclear cells (CD14+) in the period shown. Regarding result of the psoriatic patient, three cytokines abnormally prolonged after the 24 hours induction. The signals of TNF-α, IL10 and HBEGF expanded throughout 48 hours,5 days and 7 days, respectively. In addition, a band (627bp) was obtained in from PCR products of IL10. Furthermore, sequencing the 627bp band shows that it was homologous to a fragment on chromosome 2. Meanwhile, the results of those cytokines on a cohort without non-psoriasis were slightly exposed in around 48 hours. In conclusion, the mononuclear cells (CD14+) in the cohort with guttate psoriasis is highly more sensitive with a lysis of Streptococcus pneumonia than the non-psoriatic cohort in TNF-α expression, and IL10 and HBEGF of the psoriatic one are also simultaneous and extended during the inflammation and wound healing. Furthermore, the 620bp band might relate to edit progressing pre-mRNA IL10 in the guttate psoriasis.

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Comparison of Helicobacter pylori and Escherichia coli in induction of TNF-α mRNA from human peripheral blood mononuclear cells

Indian Journal of Medical Microbiology ◽

10.4103/0255-0857.66482 ◽

2010 ◽

Vol 28 (3) ◽

pp. 233 ◽

Cited By ~ 3

Author(s):

H Zarkesh-Esfahani ◽

R Roghanian ◽

FNavab Akbar ◽

E Ahmadzadeh

Keyword(s):

Escherichia Coli ◽

Helicobacter Pylori ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Peripheral Blood Mononuclear ◽

Tnf Α ◽

Blood Mononuclear Cells

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Fibrinogen and fibrin induce synthesis of proinflammatory cytokines from isolated peripheral blood mononuclear cells

Thrombosis and Haemostasis ◽

10.1160/th07-01-0039 ◽

2007 ◽

Vol 97 (05) ◽

pp. 822-829 ◽

Cited By ~ 56

Author(s):

Peter Kierulf ◽

Per Sandset ◽

Olav Klingenberg ◽

Gunn Joø ◽

Hans Godal ◽

...

Keyword(s):

Molecular Weight ◽

Acute Phase ◽

Proinflammatory Cytokines ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Low Molecular Weight ◽

Peripheral Blood Mononuclear ◽

Tnf Α ◽

Blood Mononuclear Cells

SummaryFibrinogen in plasma includes three main fractions; high-molecular- weight (HMW) -fibrinogen, low-molecular-weight (LMW) -fibrinogen, and very-low-molecular-weight (LMW`) -fibrinogen. During acute-phase conditions, plasma fibrinogen levels and the HMW-/LMW-fibrinogen ratio increase rapidly due to increased synthesis of HMW-fibrinogen. The consequences of elevated plasma fibrinogen levels and local deposition of fibrin in inflammatory tissues observed during acute-phase conditions are not clear.We wanted to investigate proinflammatory effects of fibrinogen and fibrin on peripheral blood mononuclear cells (PBMC) as reflected by altered mRNA expression and synthesis of the proinflammatory cytokines IL-6,TNF- α and IL-1 β, and to explore the significance of altered HMW-/LMW-fibrinogen ratio. PBMC were isolated from whole blood using Lymphoprep® . HMW-fibrinogen was separated from unfractioned fibrinogen by ammonium sulphate precipitation. Cells were incubated with unfractioned fibrinogen, HMW-fibrinogen or fibrin. Cytokine levels in cell lysates were determined using ELISA assays. Real-time PCR was used for mRNA quantification. We found that fibrinogen significantly increased mRNA levels, and induced synthesis of the proinflammatory cytokines IL-6 and TNF- α in PBMC in a dose dependent manner. Median (25, 75 percentile) IL-6 and TNF- α concentrations were 12 (5, 40) pg/ ml and 16 (0,61) pg/ml,respectively.Median mRNA quantity was increased 12.3– (6.6, 48.6) and 1.7– (1.5, 6.5) fold for IL-6 and TNF- α compared to controls.The stimulatory effect of unfractioned fibrinogen was not significantly different from HMW-fibrinogen. Fibrinogen and fibrin were equally effective in promoting cytokine synthesis from PBMC.The results support that fibrin and fibrinogen may actively modulate the inflammatory process by inducing synthesis of proinflammatory cytokines from PBMC.

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Modulation of Gro-α and Tnf-α Production by Peripheral Blood Mononuclear Cells Treated with Killed Helicobacter Pylori

European Journal of Inflammation ◽

10.1177/1721727x0700500204 ◽

2007 ◽

Vol 5 (2) ◽

pp. 83-88 ◽

Cited By ~ 9

Author(s):

G. Stassi ◽

A. Cascio ◽

C. Iaria ◽

D. Gazzara ◽

G.B. Costa ◽

...

Keyword(s):

Helicobacter Pylori ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Tnf Α ◽

Blood Mononuclear Cells

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Do proinflammatory cytokines play a role in clozapine-associated glycometabolism disorders?

Psychopharmacology ◽

10.1007/s00213-021-05824-9 ◽

2021 ◽

Author(s):

Tongtong Zhao ◽

Kai Zhang ◽

Yelei Zhang ◽

Yating Yang ◽

Xiaoshuai Ning ◽

...

Keyword(s):

Blood Glucose ◽

Side Effects ◽

Proinflammatory Cytokines ◽

Peripheral Blood ◽

Animal Experiments ◽

Pancreatic Tissue ◽

Mouse Serum ◽

Healthy Controls ◽

Immunological Mechanisms ◽

Tnf Α

Abstract Rationale and objective Clozapine (CLZ) is the most effective drug for treatment-resistant schizophrenia but is associated with many side effects, including glycometabolism disorders. Immunological mechanisms may be involved in the development of clozapine side effects. Research relating the immunomodulatory effects of clozapine and its early markers to clinically relevant adverse events is needed to reduce the harmful side effects of clozapine. This study aimed to investigate the role of proinflammatory cytokines in clozapine-associated glycometabolism disorders. Methods We measured the effect of a range of doses of clozapine on glycometabolism-related parameters and proinflammatory cytokines levels in mice peripheral blood. We also examined the differences between these indicators in the peripheral blood of clozapine-treated schizophrenia patients and healthy controls. Furthermore, we detected proinflammatory cytokines expression in mice pancreatic tissue. Results Following clozapine administration, glucagon significantly decreased in mouse serum, and proinflammatory cytokine IL-β levels markedly increased. Clozapine reliably increased proinflammatory cytokines (IL-1β, IL-6, and TNF-α) expression in murine pancreatic tissue. Compared with healthy controls, clozapine-treated patients’ BMI, blood glucose, and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) increased significantly. In clozapine-treated patients, a higher clozapine daily dosage was associated with higher levels of the proinflammatory cytokines IL-1β and IL-6, and a significant positive correlation was observed between blood glucose levels and the proinflammatory cytokines IL-6 and TNF-α. Conclusion Findings from animal experiments and clinical trials have shown clear evidence that clozapine has a regulatory effect on immune-related proinflammatory cytokines and influences glycometabolism indicators.

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Resistin, TNF-α, and microRNA 124-3p expressions in peripheral blood mononuclear cells are associated with diabetic nephropathy

International Journal of Diabetes in Developing Countries ◽

10.1007/s13410-021-00966-0 ◽

2021 ◽

Author(s):

Amin Monjezi ◽

Azam Khedri ◽

Mehrnoosh Zakerkish ◽

Ghorban Mohammadzadeh

Keyword(s):

Diabetic Nephropathy ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Tnf Α ◽

Blood Mononuclear Cells ◽

Microrna 124

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Immunoglobulin A1 Protease, an Exoenzyme of Pathogenic Neisseriae, Is a Potent Inducer of Proinflammatory Cytokines

Journal of Experimental Medicine ◽

10.1084/jem.190.8.1049 ◽

1999 ◽

Vol 190 (8) ◽

pp. 1049-1058 ◽

Cited By ~ 52

Author(s):

Dirk R. Lorenzen ◽

Frank Düx ◽

Uwe Wölk ◽

Anastasios Tsirpouchtsidis ◽

Gaby Haas ◽

...

Keyword(s):

Proinflammatory Cytokines ◽

Mononuclear Cells ◽

Native Form ◽

Peripheral Blood Mononuclear ◽

Potent Inducer ◽

Cytokine Induction ◽

Regulatory Cytokine ◽

Iga1 Protease ◽

Tnf Α ◽

Necrosis Factor

A characteristic of human pathogenic Neisseriae is the production and secretion of an immunoglobulin (Ig)A1-specific serine protease (IgA1 protease) that cleaves preferentially human IgA1 and other target proteins. Here we show a novel function for native IgA1 protease, i.e., the induction of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-8 from peripheral blood mononuclear cells. The capacity of IgA1 protease to elicit such cytokine responses in monocytes was enhanced in the presence of T lymphocytes. IgA1 protease did not induce the regulatory cytokine IL-10, which was, however, found in response to lipopolysaccharide and phytohemagglutinin. The immunomodulatory effects caused by IgA1 protease require a native form of the enzyme, and denaturation abolished cytokine induction. However, the proteolytic activity is not required for the cytokine induction by IgA1 protease. Our results indicate that IgA1 protease exhibits important immunostimulatory properties and may contribute substantially to the pathogenesis of neisserial infections by inducing large amounts of TNF-α and other proinflammatory cytokines. In particular, IgA1 protease may represent a key virulence determinant of bacterial meningitis.

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