Introduction. Vitamin D is intensively studied during the last years. The most useful instrument to assess the vitamin D status is serum 25-hydroxy vitamin D (25-OH D). Material and method. This is a cross sectional study in menopausal women, between 2008 and 2013 with inclusion criteria: at least 1 year since menopause, age between 40 and 80 years; exclusion criteria: specific therapy for osteoporosis, previous diagnosis of osteomalacia and rickets, primary hyperparathyroidism. Results. 471 subjects were: group 1 with osteopenia and osteoporosis (N = 328) and group 2 with normal DXA (N = 143) which were statistically significant (SS) different regarding the age, body mass index (BMI), years since menopause, bone markers osteocalcin and CrossLaps, with no SS differences between 25-OH D. In group 1: the linear regression coefficient (r) between alkaline phosphatase and 25-OH D was -0.14 (p = 0.01). In group 2: the BMI distribution showed: normal weighted subjects (BMI ≤ 24.9 kg/m2, N = 22, 15%, av. 25-OH D = 19.69 ng/mL), overweighed females (BMI = 25-29.9 kg/m2, N = 56, 39%, av. 25-OH D = 15.11 ng/mL ), obese (BMI ≥ 30 kg/m2, N = 65, 46%, av. 25-OH D = 12.11 ng/mL), with SS differences between any 2 subgroups. 25-OH D is not SS different between the subgroups based on decades of years since menopause, regardless the DXA score. Conclusion. Based on our observations, a prevalent low level of vitamin D is registered in menopausal women regardless they have or not osteoporosis and in women with normal DXA the vitamin D level is lower in obsesses versus normal or overweighed patients.