Tỷ lệ nhiễm Human Papillomavirus (HPV) ở phụ nữ đã lập gia đình tại tỉnh Nghệ An năm 2020

2021 ◽  
Vol 31 (7) ◽  
pp. 47-54
Author(s):  
Tăng Việt Hà ◽  
Võ Thị Thủy ◽  
Cao Xuân Hải ◽  
Nguyễn Thị Thảo ◽  
Nguyễn Văn Tuấn ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Dna ◽  
Realtime Pcr

Nghiên cứu thực hiện với mục tiêu xác định tỷ lệ nhiễm Human Papillomavirus (HPV) và định týp HPV nguy cơ cao gây ung thư cổ tử cung dựa trên kỹ thuật realtime PCR ở phụ nữ đã lập gia đình trong độ tuổi từ 19 - 60 tuổi tại tỉnh Nghệ An. Nghiên cứu mô tả cắt ngang trên 1200 đối tượng tại thành phố Vinh và 3 huyện của tỉnh Nghệ An từ tháng 06 năm 2019 đến tháng 12 năm 2020. Mẫu nhiễm Human Papillomavirus được xác định bằng Kit LightPower NAHPV PCR (Việt Á), các mẫu dương tính sau đó được định týp bằng kit HPV genotype 14 Real - TM Quant (Sacase-Ý). Kết quả cho thấy tỷ lệ nhiễm HPV là 6,25% (KTC 95%: 4,88% - 7,62%), trong đó thành phố là 6,93%; nông thôn là 4,67%; trung du, miền núi là 6,04% và 7,32% ở miền núi cao. Nghiên cứu ghi nhận 14 týp HPV-DNA khác nhau; 100% các týp đều thuộc nhóm nguy cơ cao và phổ biến nhất là các týp 16, 52, 18 và 58. Tỷ lệ nhiễm 1 týp là 68,5% và đồng nhiễm chiếm trên 31,5%. Tỷ lệ nhiễm HPV ở phụ nữ tại Nghệ An là 6,25% và có ở tất cả các khu vực sinh thái khác nhau.

scholarly journals Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Hana Jaworek ◽  
Vladimira Koudelakova ◽  
Ivana Oborna ◽  
Blazena Zborilova ◽  
Jana Brezinova ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Sexual Partners ◽  
Fisher Exact Test ◽  
Genotype Distribution ◽  
Heterosexual Couples ◽  
Anatomic Site ◽  
Hpv Dna ◽  
Hpv Test ◽  
Penile Swabs

Abstract Background Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual couples without HPV-associated disease. Methods Semen samples and penile swabs were collected from male partners and cervical swabs were collected from female partners of heterosexual couples treated for infertility (n = 195). Presence of HPV DNA in semen samples and cervical swabs was analyzed using the cobas® HPV Test and PapilloCheck®. Only the PapilloCheck® test was used to detect HPV in penile swabs. The genotype-specific prevalence and concordance of HPV infection not targeted by vaccine were evaluated using Fisher exact test. Results Both partners were infected with any HPV type in 13.8% (27/195) of couples and, of these couples, 55.6% (15/27) harbored at least one mutual genotype. High-risk HPV (hrHPV) genotypes were detected in 12.3% (24/195) of semen samples, 31.3% (61/195) of penile swabs, and 19.5% (38/195) of cervical swabs (P < 0.001). The most prevalent hrHPV genotype were HPV53 (2.56%; 5/195) in semen samples, HPV16 (6.67%, 13/195) in penile swabs and HPV39 (3.59%, 7/195) in cervical swabs. Low-risk (lrHPV) genotypes were detected in 5.13% (10/195) of semen samples, 15.9% (31/195) of penile swabs, and 4.10% (8/195) of cervical swabs (P < 0.001). Male sexual partners of HPV-positive women were more likely to be infected with at least one of the same HPV types than female sexual partners of HPV-positive men (34.9% vs. 17.9%, P = 0.055). Conclusions This study showed that the detection of HPV infection differ by anatomic site and gender. Regardless the anatomic site, high prevalence of HPV genital infection was found in both Czech men and women.

scholarly journals Triage of human papillomavirus infected women by methylation analysis in first-void urine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Severien Van Keer ◽  
Annina P. van Splunter ◽  
Jade Pattyn ◽  
Annemie De Smet ◽  
Sereina A. Herzog ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Host Cell ◽  
Intraepithelial Neoplasia ◽  
Underlying Disease ◽  
Hpv Infection ◽  
Molecular Testing ◽  
High Grade ◽  
Methylation Analysis ◽  
Hpv Dna ◽  
Screening Attendance

AbstractHost cell DNA methylation analysis in urine provides promising triage markers for women diagnosed with a high-risk (HR) human papillomavirus (HPV) infection. In this study, we have investigated a panel of six host cell methylation markers (GHSR, SST, ZIC1, ASCL1, LHX8, ST6GALNAC5) in cervicovaginal secretions collected within the first part of the urine void (FVU) from a referral population. Cytology, histology, and HPV DNA genotyping results on paired FVU and cervical samples were available. Urinary median methylation levels from HR-HPV (n = 93) positive women were found to increase for all markers with severity of underlying disease. Significantly elevated levels were observed for GHSR and LHX8 in relation to high-grade cervical intraepithelial neoplasia (CIN2 +; n = 33), with area under de curve values of 0.80 (95% Confidence Interval (CI) 0.59–0.92) and 0.76 (95% CI 0.58–0.89), respectively. These findings are the first to support the assertion that methylation analysis of host cell genes is feasible in FVU and holds promise as molecular, triage strategy to discern low- from high-grade cervical disease in HR-HPV positive women. Molecular testing on FVU may serve to increase cervical cancer screening attendance in hard-to-reach populations whilst reducing loss to follow-up and await further optimization and validation studies.

scholarly journals Pseudoepitheliomatous keratotic and micaceous balanitis: low-risk human papilloma virus detection in two further cases

2020 ◽  
pp. 095646242096194
Author(s):  
Marialuisa Corbeddu ◽  
Luca Pilloni ◽  
Roberta Satta ◽  
Laura Atzori ◽  
Franco Rongioletti
Keyword(s):  
Human Papillomavirus ◽  
Verrucous Carcinoma ◽  
Virus Detection ◽  
Low Risk ◽  
Hpv Testing ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Proliferative Markers ◽  
Male Patients ◽  
Papillomavirus Dna

We report two cases of histologically documented pseudoepitheliomatous keratotic and micaceous balanitis in middle-aged male patients, which showed positivity for low-risk serotype human papillomavirus DNA. To our knowledge, only one other case has been documented. Further immunohistochemical proliferative markers were performed and compared to literature findings in penile epithelial proliferations. Evolution to invasive verrucous carcinoma has been associated with absence of HPV DNA. Thus, if confirmed by further studies, HPV testing should be included in pseudoepitheliomatous keratotic and micaceous balanitis assessment to address prognosis, and management.

scholarly journals E1∧E4 Protein of Human Papillomavirus Type 16 Associates with Mitochondria

2004 ◽  
Vol 78 (13) ◽  
pp. 7199-7207 ◽  
Author(s):  
Kenneth Raj ◽  
Samuel Berguerand ◽  
Shirley Southern ◽  
John Doorbar ◽  
Peter Beard
Keyword(s):  
Human Papillomavirus ◽  
Terminal Portion ◽  
Hpv Dna ◽  
Human Papillomavirus Type 16 ◽  
Dead Box ◽  
Virion Production ◽  
Severe Reduction ◽  
Initial Binding ◽  
Binding To Mitochondria

ABSTRACT The human papillomavirus (HPV) E1∧E4 protein is the most abundantly expressed viral protein in HPV-infected epithelia. It possesses diverse activities, including the ability to bind to the cytokeratin network and to DEAD-box proteins, and in some cases induces the collapse of the former. E1∧E4 is also able to prevent the progression of cells into mitosis by arresting them in the G2 phase of the cell cycle. In spite of these intriguing properties, the role of this protein in the life cycle of the virus is not clear. Here we report that after binding to and collapsing the cytokeratin network, the HPV type 16 E1∧E4 protein binds to mitochondria. When cytokeratin is not present in the cell, E1∧E4 appears associated with mitochondria soon after its synthesis. The leucine cluster within the N-terminal portion of the E1∧E4 protein is pivotal in mediating this association. After the initial binding to mitochondria, the E1∧E4 protein induces the detachment of mitochondria from microtubules, causing the organelles to form a single large cluster adjacent to the nucleus. This is followed by a severe reduction in the mitochondrial membrane potential and an induction of apoptosis. HPV DNA replication and virion production occur in terminally differentiating cells which are keratin-rich, rigid squamae that exfoliate after completion of the differentiation process. Perturbation of the cytokeratin network and the eventual induction of apoptotic properties are processes that could render these unyielding cells more fragile and ease the exit of newly synthesized HPVs for subsequent rounds of infection.

Human papillomavirus (HPV) genotyping by HPV DNA chip in cervical cancer and precancerous lesions

2005 ◽  
Vol 15 (1) ◽  
pp. 81-87 ◽  
Author(s):  
G.-Y. Lee ◽  
S.-M. Kim ◽  
S.-Y. Rim ◽  
H.-S. Choi ◽  
C.-S. Park ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Precancerous Lesions ◽  
Dna Chip ◽  
Hpv Dna ◽  
Hpv Genotyping

The role of circumcision, tobacco, and alcohol use in genital human papillomavirus infection among men from Denmark

2021 ◽  
pp. 095646242110147
Author(s):  
Julie B Hebnes ◽  
Christian Munk ◽  
Kirsten Frederiksen ◽  
Hans O Joergensen ◽  
Thomas Iftner ◽  
...  
Keyword(s):  
Risk Factors ◽  
Sexual Behavior ◽  
Human Papillomavirus ◽  
Alcohol Use ◽  
Potential Risk ◽  
Hpv Infection ◽  
Sex Partners ◽  
Current Cigarette Smoking ◽  
Hpv Dna ◽  
Potential Risk Factors

It is crucial to understand the natural history of genital human papillomavirus (HPV) infection in men to prevent the increasing male HPV-related disease burden. We evaluated the associations between HPV infection and circumcision, smoking, and alcohol use after accounting for sexual behavior. The study included 2331 male personnel from Danish barracks. Penile swabs were tested for HPV DNA with a polymerase chain reaction assay, INNO-LiPA. All men completed a self-administered questionnaire providing data on potential risk factors for HPV such as lifestyle and sexual habits. Using multivariable logistic regression, associations between potential risk factors and HPV infection were estimated and expressed as odds ratios (ORs) with 95% confidence intervals (CI). Current cigarette smoking was associated with increased odds of HPV detection (OR = 1.2; 95% CI: 1.0–1.4), but we found no association with alcohol use in the analysis adjusted for sexual behavior. Circumcision reduced the odds of a prevalent HPV infection (OR = 0.7; 95% CI: 0.5–1.0) although not statistically significantly. Strong associations with lifetime and recent number of female sex partners were observed, but in contrast to uncircumcised men, increasing number of sex partners was not associated with higher HPV prevalence in circumcised men. In conclusion, smoking was associated with increased odds of penile HPV in men from the general population in Denmark, whereas circumcision seemed to reduce the risk. Moreover, our results indicated that there might be differences in the viral susceptibility between circumcised and uncircumcised men.

scholarly journals Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology

2014 ◽  
Vol 37 (2) ◽  
pp. 360-363 ◽  
Author(s):  
Ana Paula Rebelo Cassel ◽  
Regina Bones Barcellos ◽  
Cláudia Maria Dornelles da Silva ◽  
Sabrina Esteves de Matos Almeida ◽  
Maria Lucia Rosa Rossetti
Keyword(s):  
Human Papillomavirus ◽  
Cervical Cytology ◽  
Hpv Dna ◽  
Normal Cervical Cytology

Human papillomavirus (HPV) DNA in penile carcinomas in Argentina: Analysis of primary tumors and lymph nodes

2000 ◽  
Vol 61 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Mar�a A. Picconi ◽  
Ana M. Eij�n ◽  
Ang�lica L. Dist�fano ◽  
Silvia Pueyo ◽  
Lidia V. Alonio ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Lymph Nodes ◽  
Primary Tumors ◽  
Hpv Dna
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