scholarly journals Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Hana Jaworek ◽  
Vladimira Koudelakova ◽  
Ivana Oborna ◽  
Blazena Zborilova ◽  
Jana Brezinova ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Sexual Partners ◽  
Fisher Exact Test ◽  
Genotype Distribution ◽  
Heterosexual Couples ◽  
Anatomic Site ◽  
Hpv Dna ◽  
Hpv Test ◽  
Penile Swabs

Abstract Background Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual couples without HPV-associated disease. Methods Semen samples and penile swabs were collected from male partners and cervical swabs were collected from female partners of heterosexual couples treated for infertility (n = 195). Presence of HPV DNA in semen samples and cervical swabs was analyzed using the cobas® HPV Test and PapilloCheck®. Only the PapilloCheck® test was used to detect HPV in penile swabs. The genotype-specific prevalence and concordance of HPV infection not targeted by vaccine were evaluated using Fisher exact test. Results Both partners were infected with any HPV type in 13.8% (27/195) of couples and, of these couples, 55.6% (15/27) harbored at least one mutual genotype. High-risk HPV (hrHPV) genotypes were detected in 12.3% (24/195) of semen samples, 31.3% (61/195) of penile swabs, and 19.5% (38/195) of cervical swabs (P < 0.001). The most prevalent hrHPV genotype were HPV53 (2.56%; 5/195) in semen samples, HPV16 (6.67%, 13/195) in penile swabs and HPV39 (3.59%, 7/195) in cervical swabs. Low-risk (lrHPV) genotypes were detected in 5.13% (10/195) of semen samples, 15.9% (31/195) of penile swabs, and 4.10% (8/195) of cervical swabs (P < 0.001). Male sexual partners of HPV-positive women were more likely to be infected with at least one of the same HPV types than female sexual partners of HPV-positive men (34.9% vs. 17.9%, P = 0.055). Conclusions This study showed that the detection of HPV infection differ by anatomic site and gender. Regardless the anatomic site, high prevalence of HPV genital infection was found in both Czech men and women.

scholarly journals Distribution and Prevalence of High-Risk Human Papillomavirus Genotypes in Cervical Specimens

2020 ◽  
Vol 25 (3) ◽  
pp. 325-331
Author(s):  
Erkan Özmen ◽  
Ülkü Altoparlak ◽  
Muhammet Hamidullah Uyanık ◽  
Abdulkadir Gülen
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Age Groups ◽  
Hpv Infection ◽  
High Rate ◽  
Genotype Distribution ◽  
Hpv Dna ◽  
Hpv Test ◽  
Significant Difference ◽  
Hpv Types

Introduction: Human papillomavirus (HPV) is frequently a sexually transmitted virus and can cause cervical cancer in women. Cervical cancer is the second most common type of cancer among the developing countries. In this study, cervical HPV DNA positivity and genotype distributions were investigated in female patients living in our region and the results were compared with different studies. Materials and Methods: Between 1 July, 2017 and 1 March, 2019, 433 cervical swabs were sent to Ataturk University, Medical Faculty Hospital, Medical Microbiology Laboratory due to suspicion of HPV. Swab samples were evaluated for HPV virus using molecular (Polymerase Chain Reaction-PCR) methods. For this purpose, Xpert HPV Test (Cepheid, Inc, Sunnyvale, CA) was used to identify HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 t in a single sample. Results: Mean age of the patients ranged from 20 to 69 years, with a mean of 39.8 years (± 10.0). Positivity was detected in 62 of the 433 patients. Mean age of the positive patients was 40.2 years (± 11.3). When the positive patients were examined in terms of HPV types, the presence of HPV 16 was observed with a rate of 25.6%, while the HPV 18/45 types were found to be 9.0% in total. When patients were evaluated according to age groups, HPV DNA positivity was highest in the 25-34 age group with 38.7%. In our statistical study, there was no significant difference in HPV DNA positivity rate between the ages of 35 and under 35 years. Conclusion: This study demonstrates the prevalence and viral genotype distribution of HPV infection in women in Erzurum region. HPV type 16 is seen with a high rate in our region.

scholarly journals Type-Specific Identification of Genital Human Papillomavirus Infection in Women with Cytological Abnormality

2016 ◽  
Vol 60 (3) ◽  
pp. 211-216
Author(s):  
Hernán Vargas ◽  
Jenny P. Sánchez ◽  
Mónica L. Guerrero ◽  
Leider T. Ortiz ◽  
Dayanne M. Rodríguez ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Pap Smear ◽  
Hpv Vaccination ◽  
Hpv Infection ◽  
Epidemiological Surveillance ◽  
Molecular Technique ◽  
Genotype Distribution ◽  
Vaccination Programs ◽  
Hpv Dna ◽  
Cytological Abnormality

Objectives: To estimate the frequency of human papillomavirus (HPV) infection and the genotype distribution of HPV among women with a Pap smear showing atypical squamous cells of undetermined significance (ASC-US) attending the Program for the Detection and Control of Cervical Cancer in Bogotá, Colombia. Study Design: Cervical samples from 200 women with an ASC-US Pap smear were analyzed for the presence of HPV DNA and genotype distribution using a commercial molecular technique (Linear Array®; Roche Molecular Systems, USA). Results: HPV infection was found in 140 women (70%). High-risk HPV types were present in 46.4% of the samples; 16.4% showed a low-risk HPV type, and 37.1% showed both. Of the positive samples, 42.9% were infected with a single viral genotype, whereas 57.1% exhibited multiple HPV infections. The most common HPV genotypes were HPV 16, 53, and 52 with a prevalence of 26.4, 16.4, and 13.6%, respectively. Conclusion: The epidemiological characterization of HPV infections described in this study might guide actions for epidemiological surveillance to strengthen the program in Bogotá and to develop appropriate HPV vaccination programs.

scholarly journals Prevalence and distribution of human papillomavirus genotypes among women in northern Henan Province of China

2021 ◽  
Author(s):  
Xiangpeng Wang ◽  
Yuan Song ◽  
Xiaofei Wei ◽  
Guanyu Wang ◽  
Ruili Sun ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Age Groups ◽  
Hpv Infection ◽  
Cervical Cancer Prevention ◽  
Henan Province ◽  
Infection Prevalence ◽  
Genotype Distribution ◽  
Hpv Dna ◽  
Hpv Prevalence ◽  
Specific Hybridization

Abstract Background Human papillomavirus (HPV) infection can cause cervical and other cancers including cancer of vulva, vagina, penis, anus, or oropharynx. However, data concerning the prevalence and genotype distribution of HPV among women are limited in northern Henan Province of China. This study aimed to make an investigation on the current prevalence and genotype distribution of HPV among women and provided comprehensive data to guide HPV-based cervical cancer prevention in northern Henan Province. Methods A total of 15616 women aged 16 to 81 years who attended the department of gynecology of Xinxiang central hospital between January 2018 and December 2019 were enrolled in this study. HPV DNA was detected by PCR method followed by HPV type-specific hybridization. The overall prevalence, age-specific prevalence and genotype distribution of HPV were investigated. Results The overall HPV prevalence was 19.7% among women in northern Henan Province. Single, double and multiple HPV infections accounted for 13.7%, 4.3% and 1.8% of the total cases, respectively. HPV prevalence was 41.8% among women aged 16 to 19 years, 21.1% among women aged 20 to 29 years, 18.1% among women aged 30 to 39 years, 17.7% among women aged 40 to 49 years, 20.8% among women aged 50 to 59 years, 22.9% among women more than 60 years. HPV infection rates differed significantly across different age groups. Most infections were caused by high-risk HPV (HR-HPV) and single genotype HPV infection was the most common pattern. The most common HR-HPV genotype was HPV16, followed by HPV52, HPV58, HPV53 and HPV39. The most common low-risk HPV (LR-HPV) genotype was HPV6, followed by HPV61, HPV81, HPV54 and HPV11. Conclusions HPV infection is common among women in northern Henan Province. The highest infection prevalence was found in women less than 20 years old. The 9-valent HPV vaccine for routine vaccination is strongly recommended in northern Henan Province.

Persistence of human papillomavirus as a predictor for treatment failure after loop electrosurgical excision procedure

2007 ◽  
Vol 17 (6) ◽  
pp. 1271-1277 ◽  
Author(s):  
J. H. Bae ◽  
C. J. Kim ◽  
T. C. Park ◽  
S. E. Namkoong ◽  
J. S. Park
Keyword(s):  
Human Papillomavirus ◽  
Treatment Failure ◽  
Significant Risk ◽  
Hpv Infection ◽  
Dna Chip ◽  
Hpv Dna ◽  
Hpv Test ◽  
Loop Electrosurgical Excision ◽  
Loop Electrosurgical Excision Procedure

We aimed to investigate whether postconization human papillomavirus (HPV) DNA testing can predict treatment failure and improve the accuracy of conventional follow-up in women with high-grade cervical intraepithelial neoplasia (CIN). Between March 2001 and October 2005, 120 patients with confirmed CIN 2 or 3 were treated with loop electrosurgical excision procedure (LEEP) and were enrolled. Six patients were lost to the follow-up. Postconization follow-up was performed at every 3–6 months during the first year and then annually. Specimens were tested for the presence of HPV, using the Hybrid Capture 2 (Digene Co, Gaithersburg, MD) and HPV DNA chip (Mygene Co, Seoul, Korea) test. Persistent HPV infection was defined as persistently (two times or more) positive HPV tests with the same HPV subtype(s) at initial diagnosis. Twenty-two (19.3%) patients showed treatment failure after conization. The only significant risk factor for redevelopment of CIN after conization was persistence of the same HPV subtype (P< 0.0001). And women with recurrent or residual CIN had higher HPV load during the 6-month follow-up postconization. In conclusion, the persistence of the same HPV subtype after LEEP conization was an important predictor of treatment failure. The follow-up protocol after conization of CIN should include both cervical cytology and HPV test, and HPV DNA chip test is needed to detect a persistent HPV infection.

scholarly journals Human papillomavirus infection in nasal polyps in a Chinese population

2011 ◽  
Vol 92 (8) ◽  
pp. 1795-1799 ◽  
Author(s):  
Fei Pei ◽  
Xiao-Ping Chen ◽  
Yi Zhang ◽  
Yan Wang ◽  
Qun Chen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nasal Polyps ◽  
Hpv Infection ◽  
Clinicopathological Characteristics ◽  
Low Risk ◽  
Future Research ◽  
Genotype Distribution ◽  
Healthy Controls ◽  
Hpv Dna ◽  
Papillomavirus Infection

In order to determine the prevalence and genotype distribution of human papillomavirus (HPV) infection in patients with nasal polyps, a total of 204 patients with nasal polyps and 36 healthy controls were recruited for this study. Genomic DNA was extracted from paraffin-embedded tissue sections. HPV DNA genotyping was achieved by a flow-through hybridization and gene-chip method. HPV-positive infection was identified in 82 of 204 (40.2 %) patients, while HPV DNA was not found in healthy controls (P<0.05). Genotyping analysis showed that low-risk HPV genotype 11 was the most prevalent type of HPV in nasal polyps (45.28 %). Both single and multiple HPV genotype infections were found in these HPV-positive cases, although most (74.39 %) were infected with a single genotype. In addition, there was no correlation between HPV infection or HPV subtypes and the clinicopathological characteristics of patients, such as age, gender, number of surgery and disease course. The data from our study clearly demonstrated that HPV infection was associated with nasal polyps. Both high-risk HPV and low-risk HPV (LR-HPV) genotypes were identified in nasal polyp tissues, and LR-HPV-11 was the most prevalent type. Future research will explore the association of HPV infection with the development and progression of nasal polyps.

Human papillomavirus genotype distribution and risk factors for infection in women from a small municipality in north east Brazil

2012 ◽  
Vol 23 (9) ◽  
pp. e5-e10 ◽  
Author(s):  
F A Oliveira ◽  
V Ehrig ◽  
K Lang ◽  
J Heukelbach ◽  
M Stoffler-Meilicke ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Preventive Measure ◽  
Hpv Infection ◽  
Population Based ◽  
Genotype Distribution ◽  
Hpv Dna ◽  
Small Municipality ◽  
North East ◽  
Hpv Genotypes ◽  
Cervical Cancer Screening Programme

In order to assess the prevalence of human papillomavirus (HPV) infection, the HPV genotypes and factors associated with infection, we conducted a population-based survey in a small municipality in north east Brazil among women aged between 12 and 49 years. A questionnaire regarding socioeconomic variables, reproductive life and sexual behaviour was used, and women were examined gynaecologically, followed by collection of vaginal lavage with saline solution for HPV DNA determination. HPV DNA was detected by the Digene®SHARP SignalTM-System, and further genotyped by INNO-LiPA Genotyping System®. Of 579 women, HPV infection was present in 68 (prevalence: 11.7%; 95% CI: 9.3–14.7). The most common HPV types were 16, 31 and 74, each accounting for 14.7% of infections. Of all HPV-positive women, 35.3% showed multiple HPV genotypes. Variables independently associated with HPV infection were: ≥3 partners in life (adjusted OR [aOR]: 3.06; 95% CI: 1.68–5.60) and the use of oral contraception in the last 12 months (aOR: 2.39; 95% CI: 1.33–4.30). Previous participation in a cervical cancer screening programme was protective (aOR: 0.28; 95% CI: 0.13–0.60). HPV infection is common among women from rural Brazil, and HPV genotypes identified indicate that immunization could be an important preventive measure in this population.

Human papillomavirus in cervical screening and vaccination

2006 ◽  
Vol 110 (5) ◽  
pp. 543-552 ◽  
Author(s):  
Emma J. Crosbie ◽  
Henry C. Kitchener
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Residual Disease ◽  
Cervical Screening ◽  
Hpv Infection ◽  
Hpv Testing ◽  
Hpv Dna ◽  
Hpv Test ◽  
High Risk Hpv

Recent decades have witnessed a reduction in the incidence of cervical cancer in countries where screening programmes have achieved broad coverage. The recognized importance of high-risk HPV (human papillomavirus) infection in the aetiology of cervical cancer may introduce a role for HPV DNA testing in cervical screening programmes. Positive HPV DNA tests indicate women at risk of cervical cancer with greater sensitivity, but reduced specificity, compared with exfoliative cytology. Combining HPV testing with cytology may be useful in the triage of minor cytological abnormalities into those requiring referral to colposcopy (HPV positive) compared with those who can be safely managed by cytological surveillance (HPV negative). With its high sensitivity and high-negative-predictive value, HPV testing may also be useful for predicting treatment failure, since residual disease is very unlikely in the event of a negative HPV test. Ultimately, prevention is better than cure, and the advent of HPV prophylactic vaccines may obviate the need for population-based cervical screening programmes in the future. A multivalent vaccine administered to adolescents prior to the onset of sexual activity and boosted at regular intervals throughout their sexually active life may provide protection against type-specific HPV infection, malignant precursors and invasive cervical disease. Several large randomized placebo-controlled trials have been conducted with promising results. For those generations of women already exposed to high-risk HPV infection, therapeutic vaccines may offer advantages over conventional treatment, although much work still needs to be done.

scholarly journals Prevalence and distribution of human papillomavirus genotypes among women attending gynecology clinics in northern Henan Province of China

2022 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiangpeng Wang ◽  
Yuan Song ◽  
Xiaofei Wei ◽  
Guanyu Wang ◽  
Ruili Sun ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Henan Province ◽  
Genotype Distribution ◽  
Hpv Dna ◽  
Hpv Prevalence ◽  
Specific Hybridization ◽  
Current Prevalence ◽  
Hpv Genotypes ◽  
High Risk Hpv

Abstract Background Human papillomavirus (HPV) infection can cause cervical and other cancers, including vulva, vagina, penis, anus, or oropharynx. However, in China's northern Henan Province, data on the prevalence and genotype distribution of HPV among women attending gynecology clinics is limited. This study aimed to investigate the current prevalence and genotype distribution of HPV among women attending gynecology clinics in northern Henan Province. Methods This study included 15,616 women aged 16–81 years old who visited the Xinxiang central hospital's gynecology department between January 2018 and December 2019. HPV DNA was detected by a conventional PCR method followed by HPV type-specific hybridization, which was designed to detect 17 high-risk HPV (HR-HPV) genotypes and 20 low-risk HPV (LR-HPV) genotypes. HPV prevalence and corresponding 95% confidence intervals (95% CI) were calculated using SPSS 18.0. Results The overall HPV prevalence was 19.7% among women in northern Henan Province. Single, double, and multiple HPV infections accounted for 13.7%, 4.3%, and 1.8% of the total cases. Most infections were caused by HR-HPV (71.8%), and single genotype HPV infection (13.7%) was the most common pattern. The most common HR-HPV genotype was HPV16 (4.3%), followed by HPV52 (3.5%) and HPV58 (2.0%). The most common LR-HPV genotype was HPV6 (1.4%), followed by HPV61 (1.1%) and HPV81 (1.1%). Conclusions HPV infection is high among women attending gynecology clinics in northern Henan Province. The highest prevalence was found in women less than 20 years old. In northern Henan Province, the 9-valent HPV vaccine is strongly recommended for regular immunization.

scholarly journals Cervical human papillomavirus infection in Serbia: Risk factors, prevalence and genotype distribution in women with normal cervical cytology

2012 ◽  
Vol 64 (4) ◽  
pp. 1277-1283 ◽  
Author(s):  
Aleksandra Knezevic ◽  
Gordana Aleksic ◽  
I. Soldatovic ◽  
Ana Banko ◽  
Tanja Jovanovic
Keyword(s):  
Risk Factors ◽  
Human Papillomavirus ◽  
Young Women ◽  
Direct Sequencing ◽  
Hpv Infection ◽  
Cervical Cancer Prevention ◽  
Genotype Distribution ◽  
Normal Cytology ◽  
Hpv Dna ◽  
Hpv Genotypes

Sexual behavioral and other risk factors and the prevalence of genital human papillomavirus (HPV) infection are very important for the monitoring of HPV infection and cervical cancer prevention strategies. The purpose of this study was to determine the risk factors, prevalence of cervical HPV infection and genotype distribution among asymptomatic young women with normal cytology in Serbia. A total of 204 consenting young women were enrolled in this study and interviewed about risk factors. The presence of HPV DNA was assessed using the PCR method. HPV genotypes were identified by direct sequencing. Cervical HPV infection was detected in 19.1% of women. Out of nine identified HPV genotypes, types 16 and 52 were the most frequent. A significant association was found only between the number of sexual partners and HPV positivity (p<0.05). The obtained results showed the high prevalence of high-risk HPV types among young women in Serbia.

scholarly journals Biomolecular Study of the Correlation Between Papillomatosis of the Vulvar Vestibule in Adolescents and Human Papillomavirus

2006 ◽  
Vol 6 ◽  
pp. 628-636 ◽  
Author(s):  
Geni Beznos ◽  
Veronica Coates ◽  
Jose Focchi ◽  
Hatim A. Omar
Keyword(s):  
Human Papillomavirus ◽  
Histological Study ◽  
Hpv Infection ◽  
Fisher Exact Test ◽  
Sexually Active ◽  
Exact Test ◽  
Hpv Dna ◽  
Significant Difference ◽  
Group A ◽  
Group B

The main goal of this study was to investigate, through a biomolecular study, the correlation between papillomatosis of the vulvar vestibule and human papillomavirus (HPV) infection, as well as to establish the necessity of treatment. A total of 44 female adolescents between 12 and 18 years of age were selected through a prospective study with a confirmed diagnosis of papillomatosis of the vulvar vestibule. Vulvar biopsies were obtained for the histological and biomolecular detection of HPV DNA through polymerase chain reaction (PCR). Twenty (45%) adolescents were virgins (group A), the other 24 (55%) were sexually active. The virgin adolescents (group A) and 12 sexually active adolescents (group B) did not show cytological and/or colposcopic alteration, suggesting infection by HPV either on the cervix or vagina. These were compared with 12 other sexually active adolescents who showed cervicovaginal infection caused by HPV (group C). Fisher exact test was applied for statistical analysis of the results, considering alpha equal or less than 0.05. There was no statistically significant difference in relation to HPV DNA through PCR among virgin and sexually active adolescents in group B, however, both differed from those in group C (A + B × C:p= 0.048*). The histological study did not reveal evident signs of infection caused by HPV on vestibular papillae, besides perinuclear halos. HPV DNA was detected on vestibular papillae in 27%. Our results confirmed a scarce correlation between vestibular papillae and HPV. Thus, we consider papillomatosis of the vulvar vestibule, in most cases, to be equivalent to physiological papillomatosis and, therefore, should not be treated.

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