scholarly journals Experimental Exposure of the Mediterranean Mussels Mytilus galloprovincialis to Potentially Toxic Cyanobacteria (Synechocystis sp.) and Detection of Microcystins

2021 ◽  
Vol 47 (1) ◽  
pp. 10-18
Author(s):  
Maria KALAITZIDOU ◽  
◽  
Konstantinos PAPAGEORGIOU ◽  
Alexandros THEODORIDIS ◽  
Vangelis ECONOMOU ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Experimental Exposure ◽  
Toxic Cyanobacteria ◽  
The Mediterranean ◽  
Synechocystis Sp

scholarly journals Impacts of nanoparticles and phosphonates in the behavior and oxidative status of the mediterranean mussels (Mytilus galloprovincialis)

2021 ◽  
Author(s):  
Badreddine Sellami ◽  
Imen Bouzidi ◽  
Amor Hedfi ◽  
Mohammed Almalki ◽  
Roquia Rizk ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Oxidative Status ◽  
The Mediterranean

scholarly journals First report of detection of microcystins in farmed mediterranean mussels Mytilus galloprovincialis in Thermaikos gulf in Greece

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Maria P. Kalaitzidou ◽  
Christina I. Nannou ◽  
Dimitra A. Lambropoulou ◽  
Konstantinos V. Papageorgiou ◽  
Alexandros M. Theodoridis ◽  
...  
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
High Performance ◽  
Mytilus Galloprovincialis ◽  
Elisa Method ◽  
Northern Greece ◽  
Temporal And Spatial Distribution ◽  
Coastal Marine ◽  
Toxic Cyanobacteria ◽  
Comparison Of The Results

Abstract Background Microcystins are emerging marine biotoxins, produced by potentially toxic cyanobacteria. Their presence has been reported in aquatic animals in Greek freshwater, while data are few in marine environments. Since the climate change induces eutrophication and harmful algal blooms in coastal marine ecosystems affecting the public health, further research on microcystins’ presence in marine waters is required. The aim of this study was to examine the potential presence of microcystins in mussels Mytilus galloprovincialis in the largest farming areas in Thermaikos gulf, in Northern Greece, and to investigate their temporal and spatial distribution, adding to the knowledge of microcystins presence in Greek Mediterranean mussels. Results A 4-year microcystins’ assessment was conducted from 2013 to 2016, in farmed Mediterranean mussels M. galloprovincialis, in five sampling areas in Thermaikos gulf, in northern Greece, where the 90% of the Greek mussels’ farming activities is located. The isolation of potentially toxic cyanobacteria was confirmed by molecular methods. An initial screening was performed with a qualitative and quantitative direct monoclonal (DM) ELISA and results above 1 ng g−1 were confirmed for the occurrence of the most common microcystins-RR, -LR and -YR, by Ultra High Performance Liquid Chromatography (UHPLC) coupled with a high- resolution mass spectrometer (HRMS) (Orbitrap analyzer). Microcystin-RR and microcystin-LR were detected, while the intensity of microcystin-YR was below the method detection limit. Most samples that exhibited concentrations above 1 ng g-1 were detected during the warm seasons of the year and especially in spring. Results indicated an overestimation of the ELISA method, since concentrations ranged between 0.70 ± 0.15 ng g−1 and 53.90 ± 3.18 ng g−1, while the confirmation denoted that the levels of microcystins were 6 to 22 times lower. Conclusions Microcystin-RR and microcystin-LR were detected for the first time in mussel M. galloprovincialis, harvested from farms in Thermaikos gulf, in Central Macedonia, Greece. Their presence was linked to potentially toxic cyanobacteria. Bioaccumulation was observed in digestive gland, while the concentrations in muscles were found extremely low. Samples with levels above 1 ng g−1 were observed mostly during spring, confirming the seasonal distribution of microcystins. The comparison of the results by the ELISA and the LC-Orbitrap MS method indicated an overestimation of concentration by the ELISA method.

scholarly journals A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 649
Author(s):  
Marco Capolupo ◽  
Paola Valbonesi ◽  
Elena Fabbri
Keyword(s):  
Mytilus Galloprovincialis ◽  
Acetylcholinesterase Activity ◽  
Particle Sizes ◽  
Glutathione S Transferase ◽  
Phase Ii Metabolism ◽  
Chronic Effects ◽  
Mediterranean Mussel ◽  
Metabolism Enzyme ◽  
The Mediterranean

The ocean contamination caused by micro- and nano-sized plastics is a matter of increasing concern regarding their potential effects on marine organisms. This study compared the effects of a 21-day exposure to 1.5, 15, and 150 ng/L of polystyrene microplastics (PS-MP, 3-µm) and nanoplastics (PS-NP, 50-nm) on a suite of biomarkers measured in the Mediterranean mussel Mytilus galloprovincialis. Endpoints encompassed immunological/lysosomal responses, oxidative stress/detoxification parameters, and neurotoxicological markers. Compared to PS-MP, PS-NP induced higher effects on lysosomal parameters of general stress. Exposures to both particle sizes increased lipid peroxidation and catalase activity in gills; PS-NP elicited greater effects on the phase-II metabolism enzyme glutathione S-transferase and on lysozyme activity, while only PS-MP inhibited the hemocyte phagocytosis, suggesting a major role of PS particle size in modulating immunological/detoxification pathways. A decreased acetylcholinesterase activity was induced by PS-NP, indicating their potential to impair neurological functions in mussels. Biomarker data integration in the Mussel Expert System identified an overall greater health status alteration in mussels exposed to PS-NP compared to PS-MP. This study shows that increasing concentrations of nanoplastics may induce higher effects than microplastics on the mussel’s lysosomal, metabolic, and neurological functions, eventually resulting in a greater impact on their overall fitness.

scholarly journals Tissue-scale microbiota of the Mediterranean mussel (Mytilus galloprovincialis) and its relationship with the environment

2020 ◽  
Vol 717 ◽  
pp. 137209 ◽  
Author(s):  
Margherita Musella ◽  
Rasika Wathsala ◽  
Teresa Tavella ◽  
Simone Rampelli ◽  
Monica Barone ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Mediterranean Mussel ◽  
The Mediterranean

Styrene impairs normal embryo development in the Mediterranean mussel (Mytilus galloprovincialis)

2018 ◽  
Vol 201 ◽  
pp. 58-65 ◽  
Author(s):  
Rajapaksha Haddokara Gedara Rasika Wathsala ◽  
Silvia Franzellitti ◽  
Morena Scaglione ◽  
Elena Fabbri
Keyword(s):  
Embryo Development ◽  
Mytilus Galloprovincialis ◽  
Normal Embryo ◽  
Mediterranean Mussel ◽  
The Mediterranean

Lipids that contain arsenic in the Mediterranean mussel, Mytilus galloprovincialis

2020 ◽  
Vol 17 (3) ◽  
pp. 289 ◽  
Author(s):  
Fabiana P. Freitas ◽  
Georg Raber ◽  
Kenneth B. Jensen ◽  
António J. A. Nogueira ◽  
Kevin A. Francesconi
Keyword(s):  
High Performance ◽  
Mytilus Galloprovincialis ◽  
Trophic Levels ◽  
Food Sources ◽  
Tissue Type ◽  
Unicellular Algae ◽  
Molecular Mass Spectrometry ◽  
Mediterranean Mussel ◽  
The Mediterranean ◽  
First Time

Environmental contextAlthough arsenic-containing lipids are widespread in marine environments, their origin remains unknown. We show that the arsenolipids in a filter-feeding bivalve mollusc closely match those found in marine food sources, including unicellular algae and bacteria. The results demonstrate the role of lower trophic levels in determining the forms of arsenic found in higher organisms. AbstractArsenic-containing lipids, arsenolipids, are widely found among marine organisms, but their origin and possible biochemical roles remain unknown This work describes the diversity and abundance of arsenolipids in the digestive gland and mantle of nine specimens of the Mediterranean mussel, Mytilus galloprovincialis. By using high performance liquid chromatography (HPLC) coupled to both elemental and high-resolution molecular mass spectrometry, we identified 36 arsenolipids including arsenic derivatives of fatty acids, hydrocarbons, sugar-phospholipids and sugar-phytol; 21 of these arsenolipids were identified for the first time and included a new group comprising ether-phospholipids. The arsenic compounds in the mussels show distinct profiles depending on the tissue type, which provide insight into the arsenolipid origin. The results suggest that the presence of some arsenolipids in the mussels is from direct uptake of the compounds, presumably from food, rather than biogenesis within the mussels.

scholarly journals Biological Effects of the Azaspiracid-Producing Dinoflagellate Azadinium dexteroporum in Mytilus galloprovincialis from the Mediterranean Sea

Marine Drugs ◽  
2019 ◽  
Vol 17 (10) ◽  
pp. 595
Author(s):  
Maria Elisa Giuliani ◽  
Stefano Accoroni ◽  
Marica Mezzelani ◽  
Francesca Lugarini ◽  
Simone Bacchiocchi ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Biological Effects ◽  
Recovery Period ◽  
Nervous Activity ◽  
Recovery Phase ◽  
Normal Diet ◽  
Storage Lipids ◽  
Marine Biotoxins ◽  
Biological Responses ◽  
The Mediterranean

Azaspiracids (AZAs) are marine biotoxins including a variety of analogues. Recently, novel AZAs produced by the Mediterranean dinoflagellate Azadinium dexteroporum were discovered (AZA-54, AZA-55, 3-epi-AZA-7, AZA-56, AZA-57 and AZA-58) and their biological effects have not been investigated yet. This study aimed to identify the biological responses (biomarkers) induced in mussels Mytilus galloprovincialis after the bioaccumulation of AZAs from A. dexteroporum. Organisms were fed with A. dexteroporum for 21 days and subsequently subjected to a recovery period (normal diet) of 21 days. Exposed organisms accumulated AZA-54, 3-epi-AZA-7 and AZA-55, predominantly in the digestive gland. Mussels’ haemocytes showed inhibition of phagocytosis activity, modulation of the composition of haemocytic subpopulation and damage to lysosomal membranes; the digestive tissue displayed thinned tubule walls, consumption of storage lipids and accumulation of lipofuscin. Slight genotoxic damage was also observed. No clear occurrence of oxidative stress and alteration of nervous activity was detected in AZA-accumulating mussels. Most of the altered parameters returned to control levels after the recovery phase. The toxic effects detected in M. galloprovincialis demonstrate a clear biological impact of the AZAs produced by A. dexteroporum, and could be used as early indicators of contamination associated with the ingestion of seafood.

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