scholarly journals Effect of helminthosis on some blood parameters in pigs in Ibadan, Nigeria

2020 ◽  
Vol 47 (1) ◽  
pp. 53-57
Author(s):  
E. A. Adenaike
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Blood Parameters ◽  
Blood Parasites ◽  
Helminth Parasites ◽  
Blood Cell Count ◽  
Large White ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean

Coprological and haematological investigations were carried out on faecal and blood samples collected from 154 pigs from various locations around Ibadan metropolis. Coprological examination consisted mainly of egg floatation techniques while haematological investigations consisted of packed cell volume (PCV), red blood cell count (RBC) and white blood cell count (WBC). Microscopic examination of smears of the blood samples for protozoan blood parasites was also carried out. Faecal samples of 56 (36.36%) pigs were positive for helminth parasites while all the blood samples were negative for protozoan parasites. The eggs of helminth parasites found in this study were those of Ascaris spp., Metastrongylus spp., Globocephallus spp., Oesophagostomum spp., Paragonimus spp., Hyostrongylus spp. and Trichuris spp. The mean PCV, RBC and WBC values of pigs negative for helminth parasites were 31.66±8.30%, 10.57±4.68 x106 /ml and 16.97±7.82 x106 /ml respectively while the values for positive pigs were 29.71±8.93%, 9.81±4.84 x109 /ml and 17.34±7.24 x106 /ml respectively. Helminthosis caused a decline in the mean values of PCV and RBC count. The percentage decline in mean PCV values was 6.13%, 8.43% and 1.64% respectively for large white, large white crossed and local breeds of pigs. The percentage decline in mean RBC values was 6.97%, 8.74% and 3.01% respectively for large white, large white crossed and local breeds of pigs. Helminthosis caused an increase in the mean WBC values in the three breeds which was 2.69%, 1.22% and 0.91% respectively for large white, large white crossed and local breeds of pigs. The implications of the findings are discussed. In conclusion, helminthosis caused a decline in the mean values of PCV and RBC values resulting in anaemia in all breeds of pigs while the mean WBC count increased. However, experimental infections of various breeds of pigs of different ages by various types of parasites need to be carried out in order to confirm the trend observed in this study.

Correlation of Red Blood Cells and Platelets Parameters.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3764-3764
Author(s):  
Zoi Saouli ◽  
Georgia Kaiafa ◽  
Fotios Girtovitis ◽  
Zisis Kontoninas ◽  
George Ntaios ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Red Cell ◽  
Blood Cell Count ◽  
Distribution Width ◽  
Mean Values ◽  
Cell Parameters ◽  
The Mean

Abstract INTRODUCTION: Platelet along with red blood cell count is a part of complete blood cell count, one of the most frequent laboratory tests in medicine. Platelet distribution width, plateletcrit and mean platelet volume are three indices provided by hematological analyzers. There are few reports in literature regarding the correlation of these three parameters with red blood cell parameters. AIM: Aim of this study is to investigate the correlation between these platelets parameters and red cell parameters: hematocrit, mean corpuscular volume and red blood cell distribution width. METHODS: Three hundred and three healthy blood donor volunteers (176 men and 127 women, mean age 37,3 years) were included. None of them had any known hematological disease in the past. The parameters mentioned above were measured by the automated hematological analyzer Coulter®LH780. RESULTS: The mean values for platelets were: PCT: 0,25±0,11%, MPV: 8,11±1,94 fL and PDW: 15,89±2,74%. The mean values for their parallel red blood cell parameters were: HCT: 40,55±2,63%), MCV: 91±4,17 fL, RDW: 13,3±1,35% Statistical and regression analysis including the correlation coefficient between platelet and red cell parameters as well as Student’s t-test was carried out. CONCLUSIONS: There seems to be no significant correlation between HCT and PCT. MCV and MPV were not correlated significantly as well, indicating that red blood cell and platelet sizes are independent. But there is a statistically significant correlation between RDW and PDW (r: 0,68, p<0,01) demostrating that anisocytosis of red blood cells and platelets might occur simultaneously. Based on these observations, further more studies should be carried out for the correlation between platelets and red blood cell indices in certain disorders.

scholarly journals The relationship between some biochemical and hematological changes in type 2 diabetes mellitus

2017 ◽  
Vol 4 (11) ◽  
pp. 1760
Author(s):  
Zafer Saad Al Shehri
Keyword(s):  
Diabetes Mellitus ◽  
Blood Cell ◽  
Cell Count ◽  
Control Group ◽  
Blood Cell Count ◽  
Distribution Width ◽  
Mean Values ◽  
Lymphocyte Ratio ◽  
The Mean

Introduction: The aim of this study was to investigate various biochemical and hematological parameters in patients with type 2 diabetes mellitus (T2DM) and compare those with non-diabetic subjects (control group). Subjects: The study was conducted on 405 subjects (ages ranging from 26-65 years old; sex matched) who were classified into two groups: diabetic (n=205 subjects; males-105, females-100) and non-diabetic  subjects (n=200; males-100, females-100). The study was carried out during the period of November 2016 to April 2017 in the Department of Clinical Laboratory Sciences at the College of Applied Medical Science Al-Dawadmi, Shaqra University in Saudi Arabia (with the collaboration of the General Hospital Al-Dawadmi). Methods: The following various parameters were assessed for all subjects: body mass index (BMI), systolic and diastolic blood pressure (SBP-DBP), fasting blood sugar (FBS), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatise (ALP), total cholesterol (T. Ch), triglyceride (TG), low-density lipoprotein (LDL), high density lipoprotein (HDL), hemoglobin (HB), red blood cell count (RBC), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscle hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW), platelet count (Plt), mean platelet volume (MPV), platelet distribution width (PDW), total white blood cell count (WBC), lymphocyte count (L), neutrophil count (N),  eosinophil count (E), monocyte count (M), basophil count (B), neutrophil/lymphocyte ratio (N/L), and platelet/lymphocyte ratio (P/L). Results: The results showed an increase in the mean values of SGPT, alkaline phosphatase, urea, serum creatinine, total cholesterol, triglyceride, and LDL in the T2DM group relative to the control group. Meanwhile, the mean value of HDL was significantly decreased in the T2DM group compared to the control group (p<0.05). The mean values of hemoglobin, RBC, MCV, MCHC and MCH were significantly decreased in the T2DM group compared to the control group. In contrast, the red cell distribution width significantly increased in the T2DM group versus control group (p<0.05). The mean platelet count was not significantly changed in the T2DM group compared to the control group (p> 0.05), but the mean values of PDW and MPV were significantly higher in the T2DM group compared to the control group (p<0.05). The mean white blood cell count and differential white blood cell was significantly higher in the T2DM group compared to control group (p<0.05). Lastly, the mean neutrophil/lymphocyte ratio and platelet/lymphocyte ratio was not significantly different in the T2DM group compared to control group (p>0.05). Conclusion: In diabetes mellitus type 2 patients, certain biochemical and hematological changes are distinct from healthy subjects. It is important to follow up and monitor these parameters carefully in diabetic patients. Peer Review Details Peer review method: Single-Blind (Peer-reviewers: 02) Peer-review policy Plagiarism software screening?: Yes Date of Original Submission: 30 September 2017 Date accepted: 05 November 2017 Peer reviewers approved by: Dr. Lili Hami Editor who approved publication: Dr. Phuc Van Pham  

Haematology and serum biochemical profile of Kalahari Red goats reared in the hothumid south-west Nigeria

2020 ◽  
Vol 45 (5) ◽  
pp. 192-198
Author(s):  
N. Okwelum ◽  
W. Mustapha ◽  
B. O. Oluwatosin
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Total Protein ◽  
Haemoglobin Concentration ◽  
Reference Value ◽  
Haematological Parameters ◽  
Blood Cell Count ◽  
Mean Values ◽  
Serum Biochemical ◽  
The Mean

The aim of this study was to determine the biochemical and haematological profiles of Kalahari Red goats reared in the tropical environment. The Kalahari Red goats were imported into Nigeria from South Africa by the Federal University of Agriculture Abeokuta, Nigeria. The study was carried out on fifty animals of both sexes. The animals were managed under semi-intensive system where they were allowed to go out and graze on paddocks during some part of the day and brought back to the pens in the evening. The goats were fed with concentrate and grazed on sown pastures of Chloris gayana and Stylosanthes amata. Water Rwas given ad-libitum. The serum biochemical analysis was determined using Randox analysis kits. The sex of each animal was noted and recorded. The packed cell volume, haemoglobin concentration, red blood cell count, white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils and basophils were determined as haematological parameters while total protein, albumin, globulin, sodium, potassium, calcium and phosphorus were determined in serum analysis. The results showed that there was no significant (p>0.05) difference in all the haematological parameters determined across sex. There were significant (p<0.05) differences in total protein and albumin across sex of which the mean values were higher in the female goats. The value of the mean total protein in the female goats was 5.93±0.88 while the value was 5.31±0.23 in the male goats. It can be concluded that sex did not have any influence on the haematology but had association on only the protein component of the serum biochemistry. This result obtained can be used as a reference value for Kalahari Red goats reared in the tropics and under similar nutrition.

scholarly journals Effect of plateletpheresis on hematocrit, hemoglobin and erythrocyte count: Meta-analysis 1980–2018

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alejandro Gil-Betacur ◽  
Carmen Yulieth Mantilla-Gutiérrez ◽  
Jaiberth Antonio Cardona-Arias
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Meta Analysis ◽  
Erythrocyte Count ◽  
Blood Cell Count ◽  
Selection Processes ◽  
Negative Effect ◽  
Red Blood Cell Count ◽  
The Mean

AbstractThe effects of platelet donation by apheresis on different parameters of the erythrogram are still unclear. The objective was to meta-analyze the effect of plateletpheresis on hematocrit, hemoglobin, and erythrocyte count, with a systematic review with random effects meta-analysis of the mean difference. The PRISMA guidelines were considered, as well as 133 search strategies on four different databases. Reproducibility was guaranteed and methodological quality was evaluated. Heterogeneity was evaluated with Galbraith and DerSimonian-Laird’s, publication bias with a funnel plot and a Begg’s test, sensitivity analysis and a cumulative meta-analysis were also conducted. Eighteen (18) articles were included, 17 evaluated the effects on hematocrit in 2,564 donors; 13 on hemoglobin in 1,640 donors; and 4 on red blood cell count in 243 donors. A decrease of 2.26% (CI95% = 2.11–2.41) was observed in hematocrit, of 0.80 g/dL (CI95% = 0.75–0.86) in hemoglobin and −0.21 × 1012/L (CI95% = −0.13; −0.29) in red blood cell count. Plateletpheresis has a negative effect on the erythrogram parameters, explained by blood loss in the kits used for the procedure and cell lysis. Such evidence is relevant to secure the efficiency and safety of the procedure, improve selection processes or determine the number of donations that can be performed without affecting donors’ health.

scholarly journals Metabolic evaluation of Crioulo horses participating in competitions of 750 km

2013 ◽  
Vol 33 (12) ◽  
pp. 1471-1477 ◽  
Author(s):  
Lorena Alvariza Amaral ◽  
Millie Marchiori ◽  
Charles Ferreira Martins ◽  
Marcio Nunes Correa ◽  
Carlos Eduardo Wayne Nogueira
Keyword(s):  
Inflammatory Process ◽  
Ionized Calcium ◽  
Metabolic Responses ◽  
Total Calcium ◽  
Blood Cell Count ◽  
Metabolic Evaluation ◽  
Sodium Potassium ◽  
Blood Samples ◽  
Mean Values ◽  
The Mean

The Crioulo breed of horses performs in one of the most physically demanding equestrian competitions, the Marcha de Resistência, which is a contest in which the horses run 750 km in 15 days. The study's aim was to characterize the metabolic responses during this period. We evaluated eleven Crioulo horses in the competition, specifically, two males and nine females. Blood samples were collected 24 hours before the contest and on the 4th, 9th, 11th, 14th and 15th days of competition. We evaluated CK, AST, LDH, glucose, lactate, urea, creatinine, sodium, potassium, chloride, magnesium, total calcium, ionized calcium, total protein, hematocrit and the white blood cell count. At the end of the competition, the mean values of serum AST were 1151±358 IU/ L the mean LDH values were 7418±1695 IU/L and CK was 13,867±3998UI /L. There was a significant increase in urea, creatinine and lactate (p<0.0001). A decrease in the mean values of chloride, sodium, potassium, and total and ionized calcium was observed (p≤0.0002). An evaluation of the total leukocytes and segmented neutrophils (p≤0.0002) revealed their increased values, and decreased values were observed for hematocrit, plasma protein and total lymphocytes (p≤0.0003). The values of glucose, on average, remained constant. Based on these data, we conclude that the Marcha de Resistência competition necessitated a high muscular demand and the depletion of energy and electrolytes, suggesting an inflammatory process in the animals evaluated.

Haematological Profiles of Pigs on Different Farms Reflect Their Health Status

2021 ◽  
Author(s):  
Irena Golinar Oven ◽  
Alenka Nemec Svete ◽  
Melita Hajdinjak ◽  
Jan Plut ◽  
Marina Stukelj
Keyword(s):  
Health Status ◽  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell ◽  
Haemoglobin Concentration ◽  
Haematological Parameters ◽  
Blood Cell Count ◽  
Blood Samples ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.

Blood Parameters in Natural Populations of Trichosurus Species (Marsupialia: Phalangeridae). I. Age, Sex and Seasonal Variation in T. Caninus and T. Vulpecula. Ii. Influence of Habitat and Population Strategies of T. Caninus and T. Vulpecula.

1979 ◽  
Vol 27 (6) ◽  
pp. 913 ◽  
Author(s):  
JL Barnett ◽  
RA How ◽  
WF Humphreys
Keyword(s):  
Body Weight ◽  
Seasonal Variation ◽  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Plasma Glucose ◽  
Haemoglobin Concentration ◽  
Blood Parameters ◽  
Blood Cell Count ◽  
Red Blood Cell Count

Age, sex and seasonal related changes in body weight and blood parameters were determined for T caninus and T. vulpecula, whose distribution overlapped in part of the study area. In T. caninus age-related changes occurred in body weight, mean plasma glucose and protein concentrations and haematocrit. Seasonal changes occurred in plasma glucose and protein concentrations, haemoglobin concentration and red blood cell count. There were sexual dimorphisms in haematocrit, haemoglobin concentration and red blood cell count in both species; the mean values were greater in males. T. vulpecula also exhibited a sexual dimorphism in body weight (males > females). The only seasonal change in T. vulpecula was in haemoglobin concentration. Comparison of the species as adults showed that T. caninus had higher mean levels of body weight, haematocrit and haemoglobin concentration, while plasma lipid concentration and red blood cell counts were greater in T vulpecula. These species differences may be a reflection of the diets of the two species. In addition the measured parameters showed greater seasonal variation in T. caninus, a K-selected species, than in T. vulpecula, which is more r-selected.

Storage of blood samples at or above 33℃ leads to rapid appearance of appreciable systemic bias in platelet and mean corpuscular volume related parameters: an important pre-analytical factor in tropical conditions

2018 ◽  
Vol 48 (4) ◽  
pp. 334-339
Author(s):  
Ashish Jain ◽  
Sanchit Jain ◽  
Neha Singh ◽  
Sushant Kumar Meinia ◽  
Nilotpal Chowdhury
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Storage Temperature ◽  
Climatic Conditions ◽  
Analytical Control ◽  
Blood Cell Count ◽  
Blood Samples ◽  
Tropical Conditions ◽  
Red Blood Cell Count ◽  
The Stability

There is scant documentation of the stability of common haematological parameters on storage of blood samples under tropical conditions. K2EDTA samples in multiple vials were taken from 20 healthy blood donors, baseline readings taken, and stored at 33°C and 37°C. Readings from the stored vials were taken after 1, 3 and 6 h. The percent change against the baseline readings at each time point for each storage temperature was calculated. Platelet counts showed an unacceptable shift within 1 h at 37°C and 3 h at 33°C; red cell volume related parameters showed an unacceptable shift within 3 h at 37°C and 6 h at 33°C. Haemoglobin, red blood cell count, white blood cell count and mean corpuscular haemoglobin remained stable for 6 h at both temperatures. The unacceptable change for many parameters on storage at ≥33°C demonstrates the importance of ensuring pre-analytical control in regions experiencing such climatic conditions.

Heritability of Commonly Measured Blood Cell Phenotypes in the San Antonio Family Heart Study.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3687-3687
Author(s):  
Kevin R. Viel ◽  
Tom Howard ◽  
Joanne E. Curran ◽  
Laura Almasy ◽  
Eric K. Moses ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
San Antonio ◽  
Genetic Influence ◽  
Blood Cell Count ◽  
Distribution Width ◽  
Family Heart Study ◽  
Heart Study ◽  
The Mean ◽  
Cell Phenotypes

Abstract Heritability (h2) indicates the extent to which genes contribute to the observed inter-individual variation in a phenotype. Traits that have a high h2 may be fruitful targets of a subsequent genome screen to identify quantitative trait loci (QTL) and the genetic variants underlying them. We have estimated the heritabilities of measurements that commonly comprise a complete blood cell count (CBC) for members of the San Antonio Family Heart Study (table). The number of white blood cells per μL of blood (WBC) was not under genetic influence (h2 = 0.160, p = 0.253), however, the individual components of the differential (lymphocyte, monocyte, and granulocyte percentage) did appear to be. Red blood cell count (RBC) had the highest heritability (h2 = 0.638, p &lt; 0.001) with both the mean corpuscular volume (MCV) and the red cell distribution width (RDW) demonstrating moderate heritability. The platelet count (Plt) and the mean platelet volume (MPV) had a mild genetic influence that was nominally significant. Currently, the fourth longitudinal examination of this cohort is in progress and to date we have measurements for 154 Mexican American subjects in 16 nuclear families. If prior recruitment rate is maintained, bivariate analyses assessing pleiotropy between traits may be available for presentation. Some of these traits have been investigated previously, but for many these are the first reported h2 estimates to our knowledge. This investigation is a necessary first step to understanding the contributions of genes to these important clinical measurements and to the elucidation of the biological pathways involved in their regulation, which may lead to improved diagnostics and therapies to treat blood cell-related disorders. Heritabilities of commonly measured blood cell phenotypes Phenotype h2 (p-value) 1 Inverse normal transformed value WBC 0.163 (0.253) LY (%) 0.599 (&lt;0.001) MO (%) 0.603 (0.011) GR (%) 0.500 (0.004) RBC 0.638 (&lt;0.001) Hgb 0.283 (0.094) Hct 0.432 (0.023) MCV 0.476 (0.005) MCH 0.429 (0.011) MCHC1 0.249 (0.139) RDW1 0.422 (0.014) Plt 0.290 (0.013) MPV 0.298 (0.006)

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