scholarly journals Viscum album citotoxicity in Caco-2 cells (in vitro) analyzed by Dispersive Raman Spectroscopy

2021 ◽  
Vol 7 (23) ◽  
pp. 118-119
Author(s):  
Daniela Franco Lopes ◽  
Landulfo Silveira Jr.
Keyword(s):  
Raman Spectroscopy ◽  
Viscum Album

Viscum album : from high dilutions to phytotherapy

2021 ◽  
Vol 15 (4) ◽  
pp. 36-37
Author(s):  
Richard Blostin
Keyword(s):  
Herbal Medicine ◽  
Molecular Form ◽  
Macrophage Polarization ◽  
Basic Research ◽  
Viscum Album ◽  
Mistletoe Lectin ◽  
Immunological Parameters ◽  
Monocytic Cell ◽  
High Dilutions

Background : Besides conventional anticancer therapy, cancer patients use complementary and alternative medicine like the medicinal herb mistletoe (Viscum album L.), used in oral form or injections. For this condition Viscum album (VA) is also used in high dilutions, in injections, as in Brazil. Aim: Our aim is to compare results of basic research about these two presentations: herbal medicine and high dilutions and to look for an eventual continuity of action between the molecular form (herbal medicine) and the informative one (high dilutions) Method: About VA in herbal medicine : a lot of references in immunology, in particular those published by the team of Srini Kaveri and Stephan Baumgartner.(1) (2) (3) (4) (5) About VA in high dilutions : the work in immunology of Aloiso Cunha de Carvalho and Leoni Bonamin published in a thesis. (6) And then to compare those results with our practice as veterinarian on dogs and cats . Results : VA in herbal medecine: from basic research results, we know that in low concentration, an immunostimulary action is observed while in higher doses it is a cytotoxic activity. These two properties are found in our practical use of VA in dogs or cats : stabilization of the size of tumors for the low concentrations, necrosis of tumors and abscess for the high concentrations. VA in high dilutions (from D3 to D30) : from basic research, an immunostimutating effect is observed. More exactly, on a murin model of tumor of Ehrlich, it is observed an improvement of quality of life, a reduction of the tumor immunosuppressive effect, an enlargement of local inflammations (Oedema) for all concentrations, but only 3D reduces tumor growth, without modifying local necrosis and the immunological parameters. In our practice, on two cases of osteosarcoma on dogs, we verify those properties using VA both in herbal medicine and in high dilutions. Conclusions: From these results, both in basic research and in the practice, we should estimate there is probably a continuity of action fom VA, in High Dynamised Dilutions ( no more molecule), to VA in herbal medicine (molecular activity). From immunostimation to cytotoxicity, from information to molecular activity. Does each Viscum album’s preparation touch the same level of the Immune System ? The same cells ? The same receptors ? A lot of questions remain… References 1) Duong Van Huyen JP and al. Variable sensitivity of lymphoblastoid cells to apoptosis induced by Viscum album Qu FrF, a therapeutic preparation of mistletoe lectin. Chemotherapy. (2001) 2) Duong Van Huyen JP and al. Induction of apoptosis of endothelial cells by Viscum album: a role for anti-tumoral properties of mistletoe lectins. Mol Med. (2002) 3)Duong Van Huyen JP and al Comparative study of the sensitivity of lymphoblastoid and transformed monocytic cell lines to the cytotoxic effects of Viscum album extracts of different origin.. Chemotherapy. (2003) 4) Estko M and al. Tumour cell derived effects on monocyte/macrophage polarization and function and modulatory potential of Viscum album lipophilic extract in vitro. BMC Complement Altern Med. 2015 5) Saha C, and al Differential Effects of Viscum album Preparations on the Maturation and Activation of Human Dendritic Cells and CD4⁺ T Cell Responses. Molecules. 2016 Jul 14;21(7) 6) Aloisio Cunha de Carvalho , ATIVIDADE ANTI-NEOPLÁSICA DE Viscum album (L) EM TUMORES EXPERIMENTAIS: REVISÃO CRÍTICA E ESTUDO EXPERIMENTAL EM TUMOR DE EHRLICH, Thesis Sao Paulo

Erratum: Study on the in vitro and in vivo activation of rat hepatic stellate cells by Raman spectroscopy

2007 ◽  
Vol 12 (5) ◽  
pp. 059801
Author(s):  
Aiguo Shen ◽  
Zhangxiu Liao ◽  
Hui Wang ◽  
Iiho Goan ◽  
Yong Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Hepatic Stellate Cells ◽  
Stellate Cells

scholarly journals Current and Future Advancements of Raman Spectroscopy Techniques in Cancer Nanomedicine

2021 ◽  
Vol 22 (23) ◽  
pp. 13141
Author(s):  
Elisabetta Canetta
Keyword(s):  
Raman Spectroscopy ◽  
Imaging Techniques ◽  
Rapid Development ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cancer Diagnostics ◽  
Raman Signal ◽  
Therapeutic Effects ◽  
Cancer Nanomedicine

Raman scattering is one of the most used spectroscopy and imaging techniques in cancer nanomedicine due to its high spatial resolution, high chemical specificity, and multiplexity modalities. The flexibility of Raman techniques has led, in the past few years, to the rapid development of Raman spectroscopy and imaging for nanodiagnostics, nanotherapy, and nanotheranostics. This review focuses on the applications of spontaneous Raman spectroscopy and bioimaging to cancer nanotheranostics and their coupling to a variety of diagnostic/therapy methods to create nanoparticle-free theranostic systems for cancer diagnostics and therapy. Recent implementations of confocal Raman spectroscopy that led to the development of platforms for monitoring the therapeutic effects of anticancer drugs in vitro and in vivo are also reviewed. Another Raman technique that is largely employed in cancer nanomedicine, due to its ability to enhance the Raman signal, is surface-enhanced Raman spectroscopy (SERS). This review also explores the applications of the different types of SERS, such as SERRS and SORS, to cancer diagnosis through SERS nanoprobes and the detection of small-size biomarkers, such as exosomes. SERS cancer immunotherapy and immuno-SERS (iSERS) microscopy are reviewed.

scholarly journals Homoeopathic Viscum album (10-3) is More Cytotoxic to in vitro Culture of Human Breast Cancer Cell Line than to Human Mesenchymal Stem Cells

2021 ◽  
Vol 19 (4) ◽  
pp. 25-34
Author(s):  
Ana Catarina Viana Valle ◽  
Lana Ribeiro Aguiar ◽  
Hilana dos Santos Sena Brunel ◽  
Patricia Furtado Malard ◽  
Carla Lujan Pereira Villarroel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Line ◽  
Culture Medium ◽  
Cancer Cell Line ◽  
Viscum Album ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Mcf 7

BACKGROUND Adenocarcinomas can be of several types, and MCF-7 is an adenocarcinoma of human breast cell line useful as preclinical model to screen therapeutic agents such as ultra-diluted Viscum album, an European plant whose extract is commonly used in cancer therapy. AIMS MCF-7 and mesenchymal stem cells were used to evaluate the in vitro cytotoxicity of homoeopathic Viscum album 1x10-3 (VAD3). METHODS cells were cultured for 24 hours in controlled environment (37.5oC and 5% CO2) in 96-well plates. After this time, VAD3 was added to the culture medium in concentrations varying from 10 to 100 ?L/mL for MTT assay (evaluation of viability of cells). A control group was maintained with culture medium only. After 48 hours, the procedures of analysis of cells viability were performed. RESULTS MTT assay showed that the concentrations of 42 ?L/mL and 62 ?L/mL were able to reduce cell viability to 50% in MCF-7 and mesenchymal stem cells, respectively, which means that half of the cells cultured were dead after 48 hours in contact with VAD3. CONCLUSION Viscum album presented higher cytotoxic action on human breast cancer cell line culture than on mesenchymal stem cells. This medicine is extensively used against cancer, and the use of the homoeopathic form of it brings new possibilities as no or fewer adverse effects would be present.

Raman spectroscopy of blood in-vitro

2012 ◽  
Author(s):  
A. E. Villanueva-Luna ◽  
J. Castro-Ramos ◽  
S. Vazquez-Montiel ◽  
A. Flores-Gil ◽  
C. M. Ortiz-Lima ◽  
...  
Keyword(s):  
Raman Spectroscopy

Viscum album (L) extracts in cancer treatment: a systematic review of in vitro and in vivo studies

2021 ◽  
Vol 14 (2) ◽  
pp. 52-52
Author(s):  
Aloisio Cunha de Carvalho ◽  
Leoni Villano Bonamin
Keyword(s):  
Systematic Review ◽  
Experimental Studies ◽  
Pharmaceutical Preparations ◽  
Viscum Album ◽  
In Vitro Models ◽  
In Vivo Studies ◽  
Pubmed Database ◽  
Anti Cancer

Background: Several reviews about phytotherapy and homeopathy have been published in the last years, including Viscum album (VA.L). VA is a parasite plant whose extract has anti-cancer proprieties and is used alone or in combination with conventional chemotherapy. Methods: We performed a systematic review about the in vivo and in vitro models described in the literature, including veterinary clinical trials. The literature was consulted from Pubmed database. Results: There are several kinds of pharmaceutical preparations about VA and their active principles used in experimental studies, lectin being frequently studied (alone or as an extract compound). More than 50% of available literature about VA is related to the lectin effects. On the other hand, the effects of viscotoxins are less studied. Among the in vivo experimental studies about VA and its compounds, the B16 murine melanoma is the most used model, followed by Ehrlich, Walker and Dalton tumors. The results point to the apoptotic effects, metastasis control and tumor regression. Some veterinary clinical studies about the use of VA in the treatment of sarcoid, fibrosarcoma and neuroblastoma are quoted in literature too, with interesting results. Considering the in vitro models, our review revealed that NALM6 leukemia cells, B16 melanoma and NC1-H460 lung carcinoma were the most studied tumor models, apoptosis signals being the most important findings. Only one study verified immunoglobulin and interleukin production. All consulted papers were related to phytotherapy preparations only. Conclusions: Although the literature about the anti-cancer activity of VA extract and its lectins is enough, there is a marked lack of information about viscotoxin activities and about the effects of homeopathic preparations of this plant on animal tumors and on in vitro cultivated tumor cells.

scholarly journals Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1145 ◽  
Author(s):  
Shuvashis Dey ◽  
Matt Trau ◽  
Kevin M. Koo
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Immunotherapy ◽  
Immune Checkpoint ◽  
Checkpoint Inhibitors ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Checkpoint Receptors

Cancer immunotherapy encompasses a variety of approaches which target or use a patient’s immune system components to eliminate cancer. Notably, the current use of immune checkpoint inhibitors to target immune checkpoint receptors such as CTLA-4 or PD-1 has led to remarkable treatment responses in a variety of cancers. To predict cancer patients’ immunotherapy responses effectively and efficiently, multiplexed immunoassays have been shown to be advantageous in sensing multiple immunomarkers of the tumor microenvironment simultaneously for patient stratification. Surface-enhanced Raman spectroscopy (SERS) is well-regarded for its capabilities in multiplexed bioassays and has been increasingly demonstrated in cancer immunotherapy applications in recent years. This review focuses on SERS-active nanomaterials in the modern literature which have shown promise for enabling cancer patient-tailored immunotherapies, including multiplexed in vitro and in vivo immunomarker sensing and imaging, as well as immunotherapy drug screening and delivery.

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