Stable isotope analysis for control of declared geographic origin of Austrian and Slovak apricots: The IDARPO-Interreg-Project (AT-SK)

2020 ◽  
Author(s):  
Micha Horacek
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Fruit Juice ◽  
Isotope Composition ◽  
Isotope Analysis ◽  
Geographic Origin ◽  
Analytical Techniques ◽  
Sample Data ◽  
First Results ◽  
The Eu

<p>Food products of certain geographic origin are more valued by consumers than the same commodities from other regions. Therefore, there is the risk and fear that incorrect labelling and declaration of geographic origin can occur to increase profit. Thus, a control of declared geographic origin is required to ensure correct labelling and to identify fraud.</p><p>For this purpose, apricot samples of the recent vintage (2019) are investigated to differentiate samples from different apricot-producing regions in Austria, Slovakia and other countries. The isotope composition of the elements hydrogen (H), carbon (C), nitrogen (N) and oxygen (O) of fruit pulp (H, C, N, O), fruit stone (H, C, O) and fruit juice (O) is analysed to find appropriate parameters for the differentiation of geographic origin. The investigation of different sample tissues (pulp, stone, juice) supports a better differentiation of geographic origin due to different seasonal intervals influencing the respective commodities.</p><p>Within the frame of the project 3 vintages will be investigated and analysed for stable isotopes as well as other analytical techniques (molecular markers). The combination of all sample data (including previously accumulated data, e.g. Horacek 2017, Horacek 2019) will lead to an improved differentiation and identification of geographic origin.</p><p>This work is a contribution to the Interreg project IDARPO partially funded by the EU-Interreg program.</p><p>References:</p><p>Horacek, M., 2017, Isotope investigation of apricots from the Wachau-area/Lower Austria („Wachauer Marille“) to control the declared geographic origin: A pilot study – first results. Mitteilungen Klosterneuburg. 67, p. 219-228.</p><p>Horacek, M., 2019, Stable isotope analysis for control of declared geographic origin of Austrian apricots. EGU 2019, Vienna.</p>

Stable isotope analysis for green coffee bean: A possible method for geographic origin discrimination

2009 ◽  
Vol 22 (5) ◽  
pp. 463-471 ◽  
Author(s):  
Carla Isabel Rodrigues ◽  
Rodrigo Maia ◽  
Marco Miranda ◽  
Miguel Ribeirinho ◽  
J.M.F. Nogueira ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Geographic Origin ◽  
Coffee Bean ◽  
Green Coffee ◽  
Green Coffee Bean

scholarly journals Stable C and N isotope analysis of human dental remains retrieved from an ancient well at Ajnala (Punjab, India): Assessment of Dietary status and geographic affiliations

2021 ◽  
Author(s):  
J.S. SEHRAWAT ◽  
Deeksha Sankhyan ◽  
Som Dutt ◽  
Niraj Rai
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Geographic Origin ◽  
Uttar Pradesh ◽  
Skeletal Remains ◽  
Indian States ◽  
Study Results ◽  
C And N ◽  
Historical Accounts

Abstract Stable isotope analysis of biogenic tissues like tooth and bone has become a widely recognized and increasingly important method for provenance of human remains, particularly in bio-archaeological and forensic investigations. Establishing the dietary status and identity of unknown human skeletal remains retrieved from forensic anthropological contexts is a challenging task. Thousands of unknown human osseous remains along with the personalized contextual items, reportedly belonging to 282 Indian soldiers killed in 1857, were excavated non-scientifically from an abandoned well at Ajnala (Amritsar, India). In present study, the isotopic concentrations of carbon (C) and nitrogen (N) were estimated from the dentine collagen extracted from 21 first molars to provide information about the geographic affinity and dietary status of the individuals killed in Ajnala. As diagenesis affects bone more severely than enamel or dentine due to increased porosity of the former, so teeth were preferred to bones for stable isotope analysis in present study. The literature supported C: N range of 2.8-3.6 was considered as cut-off for the well-preserved collagen and the samples with values outside this range were considered to be altered or contaminated with non-collagenous materials. The interpretation of the obtained isotope values from Ajnala teeth samples indicated the consumption of C3/C4 mixed diet (though some samples showing marine diet) by the victims which supported the previous observations about the dietary status of Ajnala victims estimated from prevalence of various dental pathologies. Though C and N isotopes are generally not the best indicators of geographic origin, they can be used to for the purpose only if they show different dietary inputs of C3 and C4 plants. Present study results provided scientific confirmation to the written historical accounts that Ajnala skeletal remains belonged to the individuals belonging to the Indian states of Awadh (north-eastern Uttar Pradesh), Bihar and Bengal and some norteastern states.

scholarly journals The influence of sex, hormone status, and growth rate on dietary isotope fractionation: a case study with laboratory rats

2021 ◽  
Author(s):  
Jessica Santollo ◽  
Caroline Jones ◽  
Sora L Kim
Keyword(s):  
Growth Rate ◽  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Trophic Ecology ◽  
Isotope Composition ◽  
Isotope Analysis ◽  
Gonadal Hormones ◽  
Gonadal Hormone ◽  
Laboratory Rats

Stable isotope analysis is increasingly used to discern ecological differences within and among species, especially those difficult to observe. In applied ecological studies, variations in stable isotope composition are often attributed to foraging behavior or trophic ecology rather than fractionation during metabolic processes. One physiological difference among individuals is gonadal hormone levels, which affects food intake, metabolism, and locomotor activity. However, it is unclear how these effects, isolated from ecology, affect metabolic dynamics and expression in stable isotope analysis. Here, we test the linkage between gonadal hormones and isotopic heterogeneity among individuals with captive rats. We found that sex and removal of gonadal hormones are factors either individually or interactive for d15N and d13C values in serum and blood as well as d15N values of muscle and liver. Furthermore, the degree of fractionation in these tissues is related to growth rate. Gonadectomization also affected isotopic composition for liver d13C values and kidney d15N values, but fat d13C values were affected only by sex. The pattern of differentiation between groups was most different for kidney d15N and d13C values, which also had the largest isotopic variability among groups. Overall, isotopic variation within one tissue for the four groups demonstrated up to 1 ‰ difference in d13C and d15N values suggesting that sex based within population variations should into consideration these potential physiological effects.

scholarly journals Stable Isotope Analysis of Collagen in the Study of Medieval Manuscript Parchment: Interpretation of the First Results

2018 ◽  
Author(s):  
Е.В. Уханова
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
The State ◽  
Medieval Studies ◽  
Medieval Manuscript ◽  
Printed Books ◽  
First Results ◽  
Academy Of Sciences

Специфика древнерусского письменного наследия состоит в том, что бóльшая часть средневековых пергаменных кодексов XI–XIV вв. не содержит данных о месте их создания. Это обстоятельство является огромной проблемой отечественной медиевистики. Для ее решения нами была поставлена задача применить изотопный анализ коллагена (метод изотопной масс-спектрометрии) к большому комплексу древнейших пергаменных рукописей, хранящихся в Отделе рукописей Государственного исторического музея. В качестве первого объекта исследования были использованы пробы пергамена четырех новгородских кодексов последней четверти XII – последней четверти XIV в. из собрания Отдела рукописей Государственного исторического музея. Исследования двенадцати проб исторического пергамена были проведены в Институте геохимии и аналитической химии им. В. И. Вернадского (ГЕОХИ) РАН. Первые полученные результаты в области массспектрометрического анализа исторического пергамена нам кажутся вполне убедительными и перспективными для дальнейшего его применения в изучении локализации и элементов технологии изготовления средневековых рукописей. Specific features of Medieval Russia written heritage are stipulated by the fact that most medieval parchment codices do not contain data on the place where they were created. This fact is a true challenge for Russian medieval studies. To address this challenge, our task was to apply stable isotope analysis to a large corpus of the earliest parchment manuscripts kept in the Department of Manuscripts and Early Printed Books of the State Historical Museum. At first, parchment samples of four Novgorod codices dated to the last quarter of the 12th – last quarter of the 14th centuries from the collection of the Department of Manuscripts and Early Printed Books of the State Historical Museum were subjected to this analysis. The analysis of twelve samples of historical parchment was conducted in the Vernadsky Institute of Geochemistry and Analytical Chemistry of the Russian Academy of Sciences. We find the first results of the stable isotope analysis of the parchment quite conclusive and encouraging; hence, this approach can be applied in follow-up studies of the place of production and technological elements of medieval manuscript production.

scholarly journals Interactive effects of urea and lipid content confound stable isotope analysis in elasmobranch fishes

2017 ◽  
Vol 74 (3) ◽  
pp. 419-428 ◽  
Author(s):  
Aaron B. Carlisle ◽  
Steven Y. Litvin ◽  
Daniel J. Madigan ◽  
Kady Lyons ◽  
Jennifer S. Bigman ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Lipid Content ◽  
Isotope Composition ◽  
North Pacific Ocean ◽  
Lipid Extraction ◽  
Isotope Analysis ◽  
Interactive Effects ◽  
Stable Isotope Composition ◽  
Elasmobranch Fishes

Stable isotope analysis (SIA) is becoming a commonly used tool to study the ecology of elasmobranchs. However, the retention of urea by elasmobranchs for osmoregulatory purposes may bias the analysis and interpretation of SIA data. We examined the effects of removing urea and lipid on the stable isotope composition of 14 species of sharks, skates, and rays from the eastern North Pacific Ocean. While effects were variable across taxa, removal of urea generally increased δ15N and C:N. Urea removal had less influence on δ13C, whereas extracting urea and lipid generally increased δ15N, C:N, and δ13C. Because C:N values of nonextracted tissues are often used to infer lipid content and adjust δ13C, shifts in C:N following urea extraction will change the inferred lipid content and bias any mathematical adjustment of δ13C. These results highlight the importance of urea and lipid extraction and demonstrate the confounding effects of these compounds, making it impossible to use C:N of non-urea-extracted samples as a diagnostic tool to estimate and correct for lipid content in elasmobranch tissues.

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