scholarly journals A Practical and Robust Method to Compute the Boundary of Three-dimensional Axis-aligned Boxes

2014 ◽  
Keyword(s):  
Three Dimensional ◽  
Robust Method

scholarly journals Isochoric supercooled preservation and revival of human cardiac microtissues

2021 ◽  
Author(s):  
Matthew J. Powell-Palm ◽  
Verena Charwat ◽  
Berenice Charrez ◽  
Brian A Siemons ◽  
Kevin E. Healy ◽  
...  
Keyword(s):  
Pluripotent Stem Cells ◽  
Organ Preservation ◽  
Drug Exposure ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Robust Method ◽  
On Demand ◽  
Engineered Tissues ◽  
Induced Pluripotent

Low-temperature ex vivo preservation and tissue engineering based on human induced pluripotent stem cells (hiPSC) represent two of the most promising routes towards on-demand access to organs for transplantation. While these fields are often considered divergent from one another, advances in both fields present critical new opportunities for crossover. Herein we demonstrate the first-ever sub-zero centigrade preservation and revival of autonomously beating three-dimensional hiPSC-derived cardiac microtissues via isochoric supercooling, without the use of chemical cryoprotectants. We show that these tissues can cease autonomous beating during preservation and resume it after warming, that the supercooling process does not affect sarcomere structural integrity, and that the tissues maintain responsiveness to drug exposure following revival. Our work suggests both that functional three dimensional (3D) engineered tissues may provide an excellent high-content, low-risk testbed to study organ preservation in a genetically human context, and that isochoric supercooling may provide a robust method for preserving and reviving engineered tissues themselves.

A robust method for registration of three-dimensional knee implant models to two-dimensional fluoroscopy images

2003 ◽  
Vol 22 (12) ◽  
pp. 1561-1574 ◽  
Author(s):  
M.R. Mahfouz ◽  
W.A. Hoff ◽  
R.D. Komistek ◽  
D.A. Dennis
Keyword(s):  
Three Dimensional ◽  
Two Dimensional ◽  
Robust Method

scholarly journals Measurement of curvature and twist of microtubule bundles in the mitotic spindle

2021 ◽  
Author(s):  
Arian Ivec ◽  
Monika Trupinić ◽  
Iva M. Tolić ◽  
Nenad Pavin
Keyword(s):  
Mitotic Spindle ◽  
Spatial Organization ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Robust Method ◽  
Geometric Information ◽  
Simple Method ◽  
Information Characteristic ◽  
Reproducible Method ◽  
Curved Paths

AbstractThe highly ordered spatial organization of microtubule bundles in the mitotic spindle is crucial for its proper functioning. The recent discovery of twisted shapes of microtubule bundles and spindle chirality suggests that the bundles extend along curved paths in three dimensions, rather than being confined to a plane. This in turn implies that rotational forces exist in the spindle in addition to the widely studied linear forces. However, studies of spindle architecture and forces are impeded by a lack of a robust method for the geometric quantification of microtubule bundles in the spindle. In this paper, we describe a simple method for measuring and evaluating the shapes of microtubule bundles, by characterizing them in terms of their curvature and twist. By using confocal microscopy, we obtain three-dimensional images of spindles, which allow us to trace the entire microtubule bundles. For each traced bundle, we first fit a plane, and then fit a circle lying in that plane. With this easily reproducible method, we extract the curvature and twist, which represent the geometric information characteristic for each bundle. As the bundle shapes reflect the forces within them, this method is valuable for the understanding of forces that act on chromosomes during mitosis.

scholarly journals EFFICIENCY OF WATER FILTERING PROCESS DEPENDING ON THE FILTER LAYER POROSITY AND FLOW - A MATHEMATICAL MODEL

2018 ◽  
Vol 22 (4) ◽  
Author(s):  
TIRTOACA (IRIMIA) OANA ◽  
NEDEFF VALENTIN ◽  
PANAINTE-LEHADUS MIRELA ◽  
TOMOZEI CLAUDIA
Keyword(s):  
Applied Sciences ◽  
Mathematical Modeling ◽  
Mathematical Model ◽  
Experimental Approach ◽  
Three Dimensional ◽  
Filtration Efficiency ◽  
Robust Method ◽  
Intake Flow ◽  
The Mathematical Model ◽  
Filter Layer

<p>Mathematical modeling represents a challenge for any researcher or engineers that working in the field of applied sciences. The experimental approach to the water filtering process allowed achieving and verification a three-dimensional mathematical model, depending on the intake flow of the filter, the porosity of the filter layer and the nature of the filter layer. The mathematical model was developed and verified using the software Table Curve 3D. It was found that the mathematical model proposed offers a viable and robust method for determining the mechanical filtration efficiency of water.</p>

scholarly journals EFFICIENCY OF WATER FILTERING PROCESS DEPENDING ON THE FILTER LAYER POROSITY AND FLOW - A MATHEMATICAL MODEL

2016 ◽  
Vol 22 (4) ◽  
pp. 54-61
Author(s):  
OANA TIRTOACA IRIMIA ◽  
VALENTIN NEDEFF ◽  
MIRELA PANAINTE-LEHADUS ◽  
CLAUDIA TOMOZEI
Keyword(s):  
Applied Sciences ◽  
Mathematical Modeling ◽  
Mathematical Model ◽  
Experimental Approach ◽  
Three Dimensional ◽  
Filtration Efficiency ◽  
Robust Method ◽  
Intake Flow ◽  
The Mathematical Model ◽  
Filter Layer

Mathematical modeling represents a challenge for any researcher or engineers that working in the field of applied sciences. The experimental approach to the water filtering process allowed achieving and verification a three-dimensional mathematical model, depending on the intake flow of the filter, the porosity of the filter layer and the nature of the filter layer. The mathematical model was developed and verified using the software Table Curve 3D. It was found that the mathematical model proposed offers a viable and robust method for determining the mechanical filtration efficiency of water.

scholarly journals A novel approach to evaluate the effects of artificial bone focal lesion on the three-dimensional strain distributions within the vertebral body

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251873
Author(s):  
Marco Palanca ◽  
Giulia De Donno ◽  
Enrico Dall’Ara
Keyword(s):  
Fracture Risk ◽  
Vertebral Body ◽  
Three Dimensional ◽  
Focal Lesion ◽  
Digital Volume Correlation ◽  
Robust Method ◽  
Micro Computed Tomography ◽  
Stress Concentrations ◽  
Novel Approach ◽  
Before And After

The spine is the first site for incidence of bone metastasis. Thus, the vertebrae have a high potential risk of being weakened by metastatic tissues. The evaluation of strength of the bone affected by the presence of metastases is fundamental to assess the fracture risk. This work proposes a robust method to evaluate the variations of strain distributions due to artificial lesions within the vertebral body, based on in situ mechanical testing and digital volume correlation. Five porcine vertebrae were tested in compression up to 6500N inside a micro computed tomography scanner. For each specimen, images were acquired before and after the application of the load, before and after the introduction of the artificial lesions. Principal strains were computed within the bone by means of digital volume correlation (DVC). All intact specimens showed a consistent strain distribution, with peak minimum principal strain in the range -1.8% to -0.7% in the middle of the vertebra, demonstrating the robustness of the method. Similar distributions of strains were found for the intact vertebrae in the different regions. The artificial lesion generally doubled the strain in the middle portion of the specimen, probably due to stress concentrations close to the defect. In conclusion, a robust method to evaluate the redistribution of the strain due to artificial lesions within the vertebral body was developed and will be used in the future to improve current clinical assessment of fracture risk in metastatic spines.

scholarly journals Isochoric supercooled preservation and revival of human cardiac microtissues

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Matthew J. Powell-Palm ◽  
Verena Charwat ◽  
Berenice Charrez ◽  
Brian Siemons ◽  
Kevin E. Healy ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pluripotent Stem Cell ◽  
Drug Exposure ◽  
Structural Integrity ◽  
Three Dimensional ◽  
Induced Pluripotent Stem Cell ◽  
Robust Method ◽  
On Demand ◽  
Engineered Tissues ◽  
Induced Pluripotent

AbstractLow-temperature biopreservation and 3D tissue engineering present two differing routes towards eventual on-demand access to transplantable biologics, but recent advances in both fields present critical new opportunities for crossover between them. In this work, we demonstrate sub-zero centigrade preservation and revival of autonomously beating three-dimensional human induced pluripotent stem cell (hiPSC)-derived cardiac microtissues via isochoric supercooling, without the use of chemical cryoprotectants. We show that these tissues can cease autonomous beating during preservation and resume it after warming, that the supercooling process does not affect sarcomere structural integrity, and that the tissues maintain responsiveness to drug exposure following revival. Our work suggests both that functional three dimensional (3D) engineered tissues may provide an excellent high-content, low-risk testbed to study complex tissue biopreservation in a genetically human context, and that isochoric supercooling may provide a robust method for preserving and reviving engineered tissues themselves.

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