Label-free Immobilization of Nano-particles on Silicon based Electrodes for Single-biomolecule Studies

2014 ◽  
Keyword(s):  
Nano Particles ◽  
Label Free ◽  
Silicon Based

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

TOWARDS SENSING SINGLE OR A FEW BIO-MOLECULAR ARCHITECTURES: DESIGN OF FUNCTIONAL SURFACES

2008 ◽  
Vol 18 (01) ◽  
pp. 187-194
Author(s):  
PEIJI ZHAO ◽  
DWIGHT WOOLARD ◽  
JORGE M. SEMINARIO ◽  
ROBERT TREW
Keyword(s):  
Density Functional ◽  
High Sensitivity ◽  
Lower Sensitivity ◽  
Label Free ◽  
Dna Molecules ◽  
Surface Attachment ◽  
Biomolecular Sensing ◽  
Aromatic Molecules ◽  
Electrical Sensing ◽  
Silicon Based

There is considerable interest in electrical sensing of biomolecular binding since it has the potential to be label free, to work easily in aqueous environments native to the biomolecules, and to be integrated with small, fast, and inexpensive microelectronoics as detection instrumentation. Although electrochemical methods have been used successfully in detections of DNA molecules with Ag labels at very high sensitivity (~ p ml), detection of DNA molecules in terms of label free techniques has a lower sensitivity (~ μ ml). Here, the surface attachment chemistry is critical towards the detection of ultra-low concentration of biomolecules. In this article, based on density functional theory, we have calculated and analyzed the electrical characteristics of the contact between aromatic molecules and silicon (100) − 2×1 surfaces. Design principles for silicon based electrodes of electrochemically biomolecular sensing instruments for label-free sensing of single or a few biomolecular molecules have also been discussed.

Rapid and label-free optical assay of S-layer protein with high sensitivity using TiO2-coated porous silicon-based microfluidic biosensor

2020 ◽  
Vol 321 ◽  
pp. 128524 ◽  
Author(s):  
Siqi Zhuo ◽  
Mingyue Xun ◽  
Mingda Li ◽  
Xiaoxue Kong ◽  
Rui Shao ◽  
...  
Keyword(s):  
Porous Silicon ◽  
High Sensitivity ◽  
Label Free ◽  
Microfluidic Biosensor ◽  
Optical Assay ◽  
Silicon Based

A silicon-based electrochemical sensor for highly sensitive, specific, label-free and real-time DNA detection

2013 ◽  
Vol 24 (44) ◽  
pp. 444012 ◽  
Author(s):  
Yuanyuan Guo ◽  
Shao Su ◽  
Xinpan Wei ◽  
Yiling Zhong ◽  
Yuanyuan Su ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Real Time ◽  
Dna Detection ◽  
Label Free ◽  
Highly Sensitive ◽  
Silicon Based ◽  
Specific Label

Specific and label-free immunosensing of protein-protein interactions with silicon-based immunoFETs

2019 ◽  
Vol 132 ◽  
pp. 143-161 ◽  
Author(s):  
Ie Mei Bhattacharyya ◽  
Shira Cohen ◽  
Awad Shalabny ◽  
Muhammad Bashouti ◽  
Barak Akabayov ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Label Free ◽  
Protein Protein Interactions ◽  
Silicon Based

Fabrication and characterization of a porous silicon based microarray for label-free optical monitoring of biomolecular interactions

2010 ◽  
Vol 107 (1) ◽  
pp. 014513 ◽  
Author(s):  
Ilaria Rea ◽  
Annalisa Lamberti ◽  
Ivo Rendina ◽  
Giuseppe Coppola ◽  
Mariano Gioffrè ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Biomolecular Interactions ◽  
Optical Monitoring ◽  
Label Free ◽  
Fabrication And Characterization ◽  
Silicon Based
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