scholarly journals Determining the indentity of the enemy in the directives of the Main headquarters and the High command of the Army of Republic of Srpska

2020 ◽  
Vol 3 (3) ◽  
pp. 268-281
Author(s):  
Mesud Šadinlija ◽  
Keyword(s):  
Bosnia And Herzegovina ◽  
Methodological Approach ◽  
Historical Science ◽  
Historical Sources ◽  
Open Questions ◽  
Selective Approach ◽  
Yugoslav Wars ◽  
Mutual Relations

Among the numerous open questions of contemporary historiography regarding the breakdown of Yugoslavia and its consequences, the issues connected to the post-Yugoslav wars still cause the most disputes. Even in the case of the war in Bosnia and Herzegovina, waged from 1992 to 1995, all of the important questions concerning its causes, character and consequences, are still considered open, whereby the dubious interpretative discourses are often constructed on the basis of an approach which is founded on the narrowing of the jointly observed historical phenomena. The attempts to construct and legitimize the corresponding particular interpretations of the character of the war in Bosnia and Herzegovina through a narrow and selective approach applied in the identifications of the warring sides and their mutual relations are not rare. In such situations the most reliable methodological approach in the framework of historical science is to return the focus from the level of interpretation to the historical sources, their analysis, determining the authenticity and relevance for the collection of issues in question. In this work we will dedicate our attention to the directives of the Main Headquarters and the High Command of the Army of Republic of Srpska issued during 1992-1995 in those parts in which these documents perform the identification and determination of the enemy.

Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture

2020 ◽  
Vol 36 (3) ◽  
pp. 82-89
Author(s):  
O.V. Gromova ◽  
O.S. Durakova ◽  
S.V. Generalov ◽  
L.F. Livanova ◽  
O.A. Volokh
Keyword(s):  
Cell Culture ◽  
Vibrio Cholerae ◽  
Production Control ◽  
Specific Activity ◽  
Methodological Approach ◽  
Toxin Production ◽  
Submerged Cultivation ◽  
Vaccine Production ◽  
Antigenic Activity

Том 36(2020) №3 стр. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89А.В. Гаева1*, О.В. Громова1, О.С. Дуракова1, С.В. Генералов1, Л.Ф. Ливанова1, О.А. Волох1 Определение специфической активности компонентов холерной химической вакцины с использованием культуры клеток 1ФКУЗ «Российский научно-исследовательский противочумный институт «Микроб»» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Саратов 410005 *[email protected] Поступила - 2019-11-26; После доработки - 2020-03-16; Принята к публикации - 2020-05-15 Список литературы Описаны методы определения динамики продукции токсинов штаммом Vibrio cholerae 569B при глубинном культивировании в биореакторе и антигенной активности специфической фракции холерогена-анатоксина по анатоксинсвязыванию с использованием клеточных культур. Показана высокая степень соответствия результатов, полученных методами, применяемыми для контроля этапов производства холерной химической вакцины и рассмотренными в данной работе. Отмечено, что применение клеточной линии СНО-К1 наиболее перспективно для замены биомоделей на промежуточных этапах контроля активных компонентов холерной химической вакцины. Разработанный методический подход впервые предлагается использовать на этапах производства холерной бивалентной химической вакцины. культура клеток, Vibrio cholerae, холерная химическая вакцина, контроль производства, холера. Vol 36(2020) N 3 p. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89A.V. Gaeva1*, O.V. Gromova1, O.S. Durakova1, S.V. Generalov1, L.F. Livanova1, O.A. Volokh1 Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture 1Russian Research Anti-Plague Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Saratov, 410005 *[email protected] Received - 26.11.2019; Accepted - 15.05.2020 References The methods has been described to determine the dynamics of toxin production by the Vibrio cholerae 569B strain during submerged cultivation in bioreactor and of the antigenic activity of specific choleragen anatoxin fraction by anatoxin binding levels using cell cultures. High degree of consistency was observed between the results obtained via the method under consideration and those obtained via control methods at different stages of cholera chemical vaccine production. It was shown that the CHO-K1 cell line is the most promising substitute for biomodels at the intermediate stages of control of active cholera chemical vaccine components. The developed methodological approach was first proposed for use at the stages of cholera chemical bivalent vaccine manufacturing. cell culture, Vibrio cholerae, cholera chemical vaccine, production control, cholera.

scholarly journals Managing pH of Organic Matrices and New Commercial Substrates for Ornamental Plant Production: A Methodological Approach

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 851
Author(s):  
Sonia Cacini ◽  
Sara Di Lonardo ◽  
Simone Orsenigo ◽  
Daniele Massa
Keyword(s):  
Methodological Approach ◽  
Buffering Capacity ◽  
Ornamental Plant ◽  
Plant Production ◽  
Climatic Chamber ◽  
Step Procedure ◽  
Coir Dust ◽  
Organic Matrices ◽  
Substrate Mixtures

Professional peat-free substrates for ornamental plant production are increasingly required by nursery growers. Most promising materials are green compost, coconut coir dust, and woody fibre, used alone or in mixtures. One of the major concerns is pH, usually higher than optimal. In this work, a method based on a three-step procedure was adopted to acidify three organic matrices alone or in mixtures and to individuate the most suitable product, between iron(II) sulphate 7-hydrate and elemental sulphur chips. Firstly, the determination of the buffering capacity by dilution with sulphuric acid was carried out to determine dosages. Afterwards, an incubation trial of 84 (iron(II) sulphate) or 120 days (sulphur chips) was conducted on matrices and substrate mixtures with calculated doses in a climatic chamber maintained at 21 °C. Iron(II) sulphate resulted not suitable because it caused a rapid, but not lasting, pH lowering and an excessive electrical conductivity (EC) increase. Sulphur chips could instead guarantee an adequate and lasting pH lowering. These results were then validated in the open field trial on matrices and substrates. The proposed acidification methodology could be considered in developing new substrates, but the rapidity of pH acidification and EC increase on plant and mineral nutrition should be further investigated.

Bicarbonate and CO2 transport across the skin of the leopard frog, Rana pipiens: effect of PGF2alpha

1997 ◽  
Vol 272 (2) ◽  
pp. R640-R647 ◽  
Author(s):  
O. A. Candia ◽  
T. Yorio
Keyword(s):  
Methodological Approach ◽  
Transport Processes ◽  
Leopard Frog ◽  
Bicarbonate Transport ◽  
Amphibian Skin ◽  
Acid Base ◽  
Reliable Determination ◽  
Net Flux ◽  
Unidirectional Fluxes

The amphibian skin represents an important organ for osmoregulation and, like the mammalian kidney, maintains acid-base balance by secreting protons or base. However, the lack of a reliable and accurate method to measure the contribution of unidirectional fluxes of HCO3- ions to this mechanism has been an obstacle for the determination of the role of bicarbonate in epithelial acid-base homeostasis. Recently, one of us developed a method that allows for the reliable determination of transepithelial fluxes of bicarbonate, and this method was applied to determine unidirectional fluxes of (14)CO2 and H(14)CO3 under a variety of conditions. We report that the combined CO2 and HCO3- mucosal-to-serosal flux under 5% CO2 was 40% larger than the opposing flux, giving a net flux in the mucosal-to-serosal direction. This net flux was inhibited by acetazolamide. In CO2-free conditions, there was no detectable net flux; however, acetazolamide and PGF(2alpha) attenuated the mucosal-to-serosal flux and established an apparent secretion of HCO3-. A model is presented that depicts twelve vectors or components to the CO2 plus HCO3- fluxes in the frog skin. This model can accurately reproduce the experimental values measured from unidirectional fluxes of CO2 and HCO3- under a variety of conditions and can explain the effects of PGF(2alpha) on unidirectional 14C-labeled fluxes as a consequence of inhibition of H+ secretion to the apical bath, similar to what was previously suggested by our laboratory using a different methodological approach. The present method, utilizing radiolabeled HCO3-, may be useful as a means to evaluate the mechanism of action of hormones and drugs that may regulate acid-base homeostasis by altering proton and bicarbonate transport processes.

Determination of diffusion and convective transfer coefficients in food drying revisited: A new methodological approach

2017 ◽  
Vol 162 ◽  
pp. 30-39 ◽  
Author(s):  
Francisco J. Arranz ◽  
Tatiana Jiménez-Ariza ◽  
Belén Diezma ◽  
Eva C. Correa
Keyword(s):  
Methodological Approach ◽  
Transfer Coefficients ◽  
Convective Transfer

scholarly journals КЛАСТЕРИ ПІДПРИЄМСТВ: ВІРТУАЛЬНІСТЬ ТА ПРАКТИКА ЗАСТОСУВАННЯ

2019 ◽  
pp. 82-89
Author(s):  
Т. В. Калінеску
Keyword(s):  
National Economy ◽  
Economic Effects ◽  
Economic Systems ◽  
Mutual Relations ◽  
Basic Hypothesis ◽  
Competitive Products ◽  
Methodological Aspects ◽  
Further Development

The modern stage the development of Ukraine economy is require the reasonable methodological aspects to determination of effectiveness and authenticity the existence of clusters, its the applied value and virtual for the purpose of further aspects of development the theoretical principles of management enterprises in the conditions the modern progress of national economy trends. A research purpose is mark research of practice the using of clusters in modern Ukrainian reality, exposure of its virtual constituents, evaluation the role of clustering for development of theory practices the management of enterprises and its influence an economy on the competitiveness of national economy and maintenance of motion to the European and world society. As an object of research economic activity of enterprises the different industries comes forward economies of Ukraine, which are in the search of new directions of innovative development on the basis of creation of different types of clusters. Methods and principles of scientific cognition of general and special methods and receptions became methodological basis of research that the effective used in the process of ground and the applied value of the use of clustering. Including the methods of systematization and generalization? what are used for the analysis of the existent  aspects of creation the clusters in different industries of activity of enterprises; inductions and deductions, what are use for the ground of the applied value of clustering; between disciplinary analogy, what are use for the evaluation the virtual of application and adaptation of clusters to the operating of modern enterprises conditions. Supposition became the basic hypothesis of research, that modern cluster educations in different industries of Ukraine national economy have more virtual than practical application, and - basic its further development its legislatively-normative determination and use must become in the direction of creation the competitive products. The statement of basic materials. It is well-proven that the applied methods of clustering have likeness with the reliable methods of realization of prognosis, when the variety of the possible states of the economic systems is planned. Common reliable idea about development does not give reliable information a cluster about development of single enterprises in it, that is why conduct each in such cluster associations more casual character, than reliable activity to the cluster, has yet a subject. There is authenticity of that not every subject will be consistently and to move on a scenario which is offered in a cluster association, but will incarnate that which answers its idea about prosperity and necessities. In-process of expose was wrote the mechanisms of presentation the participants of cluster about a utility, satisfaction system of values, which is carried in itself by creation of different clusters. Offered approach consideration about cluster society have originality and practical meaningfulness and allow to send processes creation of clusters on determination of compatible values and necessities for its participants and receipt of public economic effects from it. The conducted research allowed to formulate a conclusion, that clustering have the hidden potentials, that not allow effective develop the economy of enterprises, but take in attention the virtual features of perception the clustering, it follows to pay attention to necessity: creation of legislatively-normative base in relation to functioning of clusters, presence of instruments the state support of activity of clusters on regional and national levels for creation of competitive products; creation of compatible values and requirements in mutual relations in a middle clusters and receipt of the objective applied effects in relation to functioning of clustering.

scholarly journals New types of usury off enses (Article 304 § 2 and 3 CC) and the ultima ratio principle of criminal law

2021 ◽  
Vol 57 ◽  
pp. 33-56
Author(s):  
Jacek Giezek ◽  
Konrad Lipiński
Keyword(s):  
Criminal Law ◽  
Civil Law ◽  
Mutual Relations

The authors analyze new types of offenses of Art. 304 § 2 and 3 CC, focusing on the issue of normative structures derived from criminal law (and other branches of law) and their mutual relations. Assuming that due to the principle of subsidiarity (ultima ratio) of criminal law, it is not possible to prohibit conduct previously not deemed unlawful by provisions of civil law, the authors conclude that the condition of liability for new “usury crimes” is determination of unlawfulness of the demanded interest or other costs of the loan, assessed from the perspective of civil law.

Obuke nastavnika kao doprinos jačanju kapaciteta za razvoj osnovnog obrazovanja odraslih u Bosni i Hercegovini

2021 ◽  
Vol 20 (1_2 2020) ◽  
pp. 57-78
Author(s):  
Violeta Orlović Lovren ◽  
Jovan Miljković ◽  
Svjetlana Tubić
Keyword(s):  
Adult Education ◽  
International Cooperation ◽  
Bosnia And Herzegovina ◽  
Methodological Approach ◽  
Political Context ◽  
Project Activity ◽  
Education Association ◽  
Insight Into ◽  
German Adult

The paper is dedicated to the analysis of the effects from teacher trainings which were developed and applied in Bosnia in Herzegovina (B&H), as a project activity within the larger project titled ''Support for adult education: subsequent acquisition of elementary adult education'', which was implemented during 2012-2013 by the GIZ and the Institute for International Cooperation of German Adult Education Association (DVV International), Office in Bosnia and Herzegovina. The reasons for initiating this training and the analysis of its effects are considered in a specific socio-historical and educational-political context, in order to analyze not only the inherited problems of illiteracy, but also the conditions in which the capacity for their resolution and building of a system of adult education are developing today. The methodological approach and findings of the research are presented, which is based on estimates of the teachers on the effects of the training in which they participated. Bearing in mind the insight into the context and findings of the research, possible directions for improvement in this field are suggested.

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