scholarly journals Managing pH of Organic Matrices and New Commercial Substrates for Ornamental Plant Production: A Methodological Approach

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 851
Author(s):  
Sonia Cacini ◽  
Sara Di Lonardo ◽  
Simone Orsenigo ◽  
Daniele Massa

Professional peat-free substrates for ornamental plant production are increasingly required by nursery growers. Most promising materials are green compost, coconut coir dust, and woody fibre, used alone or in mixtures. One of the major concerns is pH, usually higher than optimal. In this work, a method based on a three-step procedure was adopted to acidify three organic matrices alone or in mixtures and to individuate the most suitable product, between iron(II) sulphate 7-hydrate and elemental sulphur chips. Firstly, the determination of the buffering capacity by dilution with sulphuric acid was carried out to determine dosages. Afterwards, an incubation trial of 84 (iron(II) sulphate) or 120 days (sulphur chips) was conducted on matrices and substrate mixtures with calculated doses in a climatic chamber maintained at 21 °C. Iron(II) sulphate resulted not suitable because it caused a rapid, but not lasting, pH lowering and an excessive electrical conductivity (EC) increase. Sulphur chips could instead guarantee an adequate and lasting pH lowering. These results were then validated in the open field trial on matrices and substrates. The proposed acidification methodology could be considered in developing new substrates, but the rapidity of pH acidification and EC increase on plant and mineral nutrition should be further investigated.

2021 ◽  
Vol 3 (1) ◽  
pp. 24-30
Author(s):  
Farah Chalida Hanoum

This research aims to obtain an overview of rentals characteristic business ornamental plant of the scale of households and small scales and knowing the contribution rentals ornamental plant of the scale of households and small scales against income producers ornamental plants. The determination of the location of the research done deliberately (purposive) that is implemented in the city of Malang and Batu city with the consideration that location  is an ornamental plant production center to continuously ornamental plants rental. Methods of data retrieval method using Census data, the analysis carried out qualitatively and quantitatively. The result of qualitative analysis : First , in the matter of pricing, where the scale of ornamental plants rental households, no price on the basis  of plants, but based on the sized manufacturing, while on a small scale in addition to rental based on size is also based on the type of plants. Second, the targert market or tenant. Where on a small scale, ornamental plants rental market target is broader this is due to the partnership business, based on the result, the contribution of revenue each tenancy. The contribution rental income an ornamental against producers an ornamental received household scale of 33,26 percent. While on small scale of 22,90 percent.


2020 ◽  
Vol 36 (3) ◽  
pp. 82-89
Author(s):  
O.V. Gromova ◽  
O.S. Durakova ◽  
S.V. Generalov ◽  
L.F. Livanova ◽  
O.A. Volokh

Том 36(2020) №3 стр. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89А.В. Гаева1*, О.В. Громова1, О.С. Дуракова1, С.В. Генералов1, Л.Ф. Ливанова1, О.А. Волох1 Определение специфической активности компонентов холерной химической вакцины с использованием культуры клеток 1ФКУЗ «Российский научно-исследовательский противочумный институт «Микроб»» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Саратов 410005 *[email protected] Поступила - 2019-11-26; После доработки - 2020-03-16; Принята к публикации - 2020-05-15 Список литературы Описаны методы определения динамики продукции токсинов штаммом Vibrio cholerae 569B при глубинном культивировании в биореакторе и антигенной активности специфической фракции холерогена-анатоксина по анатоксинсвязыванию с использованием клеточных культур. Показана высокая степень соответствия результатов, полученных методами, применяемыми для контроля этапов производства холерной химической вакцины и рассмотренными в данной работе. Отмечено, что применение клеточной линии СНО-К1 наиболее перспективно для замены биомоделей на промежуточных этапах контроля активных компонентов холерной химической вакцины. Разработанный методический подход впервые предлагается использовать на этапах производства холерной бивалентной химической вакцины. культура клеток, Vibrio cholerae, холерная химическая вакцина, контроль производства, холера. Vol 36(2020) N 3 p. 82-89; DOI 10.21519/0234-2758-2020-36-3-82-89A.V. Gaeva1*, O.V. Gromova1, O.S. Durakova1, S.V. Generalov1, L.F. Livanova1, O.A. Volokh1 Determination of Specific Activity of Cholera Chemical Vaccine Components using Cell Culture 1Russian Research Anti-Plague Institute «Microbe» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Saratov, 410005 *[email protected] Received - 26.11.2019; Accepted - 15.05.2020 References The methods has been described to determine the dynamics of toxin production by the Vibrio cholerae 569B strain during submerged cultivation in bioreactor and of the antigenic activity of specific choleragen anatoxin fraction by anatoxin binding levels using cell cultures. High degree of consistency was observed between the results obtained via the method under consideration and those obtained via control methods at different stages of cholera chemical vaccine production. It was shown that the CHO-K1 cell line is the most promising substitute for biomodels at the intermediate stages of control of active cholera chemical vaccine components. The developed methodological approach was first proposed for use at the stages of cholera chemical bivalent vaccine manufacturing. cell culture, Vibrio cholerae, cholera chemical vaccine, production control, cholera.


1997 ◽  
Vol 272 (2) ◽  
pp. R640-R647 ◽  
Author(s):  
O. A. Candia ◽  
T. Yorio

The amphibian skin represents an important organ for osmoregulation and, like the mammalian kidney, maintains acid-base balance by secreting protons or base. However, the lack of a reliable and accurate method to measure the contribution of unidirectional fluxes of HCO3- ions to this mechanism has been an obstacle for the determination of the role of bicarbonate in epithelial acid-base homeostasis. Recently, one of us developed a method that allows for the reliable determination of transepithelial fluxes of bicarbonate, and this method was applied to determine unidirectional fluxes of (14)CO2 and H(14)CO3 under a variety of conditions. We report that the combined CO2 and HCO3- mucosal-to-serosal flux under 5% CO2 was 40% larger than the opposing flux, giving a net flux in the mucosal-to-serosal direction. This net flux was inhibited by acetazolamide. In CO2-free conditions, there was no detectable net flux; however, acetazolamide and PGF(2alpha) attenuated the mucosal-to-serosal flux and established an apparent secretion of HCO3-. A model is presented that depicts twelve vectors or components to the CO2 plus HCO3- fluxes in the frog skin. This model can accurately reproduce the experimental values measured from unidirectional fluxes of CO2 and HCO3- under a variety of conditions and can explain the effects of PGF(2alpha) on unidirectional 14C-labeled fluxes as a consequence of inhibition of H+ secretion to the apical bath, similar to what was previously suggested by our laboratory using a different methodological approach. The present method, utilizing radiolabeled HCO3-, may be useful as a means to evaluate the mechanism of action of hormones and drugs that may regulate acid-base homeostasis by altering proton and bicarbonate transport processes.


2017 ◽  
Vol 162 ◽  
pp. 30-39 ◽  
Author(s):  
Francisco J. Arranz ◽  
Tatiana Jiménez-Ariza ◽  
Belén Diezma ◽  
Eva C. Correa

2021 ◽  
Author(s):  
Tamara Erceg ◽  
◽  
Gaja Brakus ◽  
Dejan Kojić ◽  
Nevena Vukić ◽  
...  

Chitosan based hydrogel was synthesized via free-radical polymerization in a two-step procedure, after modification of chitosan by acrylic acid. Dynamic swelling test was carried out in distilled water at room temperature. Swelling kinetics was modeling using the principle of assuming the equation for a swelling ratio-time empirical dependence. Using regression analysis in this way gives the possibility for simpler determination of swelling ratio-time empirical dependence, knowing the graph functions. Obtained hydrogel was used for preparation of new bilayer hydrogel system which has potential application in wound dressing systems and other fields where pH sensitivity and improved mechanical properties of biopolymers are required in framework of green, cost-effective process.


Author(s):  
Ashraf Ragab Mohamed

Corrosion of reinforcement is considered as the major cause of most deteriorated concrete structures. As reinforcement corrodes, the load carrying capacity is affected and hence, the probability of failure increases. At the time of inspection of deteriorated structures, engineers are faced with the problem whether the available steel is enough to secure the safety of the structural member. This chapter addresses this problem based on reliability-based approach to evaluate the safety of the deteriorated members due to uniform corrosion under bending. A methodology is proposed to facilitate the determination of the member reliability index based on basic material properties and current loading. A step-by-step procedure is proposed based on charts developed in this study according to the Egyptian code provisions.


1979 ◽  
Vol 49 ◽  
pp. 93-93
Author(s):  
William D. Cotton ◽  
Jill J. Wittels

AbstractA two step procedure is developed for the determination of the angular structure of the compact components of radio sources from observations made with very long baseline interferometry (VLBI). The first step is to fit a model composed of circularly symmetric Gaussian components to the observations. Then this, or any other, model and closure phase information is used to predict visibility phases which, when paired with the corresponding visibility amplitude observation, are combined in a direct synthesis; the resulting map is deconvolved from the point source response by means of the “CLEAN” procedure. If necessary, the “cleaned” components can be used as a starting model to predict visibility phases and the process iterated. The initial model in many cases may be as simple as a point source. Convergence of this process appears to be quite rapid, requiring only a few iterations when the source is simple or the starting model is good. These two steps for source mapping can be used either together or separately. Examples are given using data generated from test models.


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