Curcumin inhibits cervical cancer cell proliferation, migration and invasion by suppressing the PI3K/AKT signaling pathway via the miR-29b/KDM2A axis

AbstractPR domain zinc finger protein 4 (PRDM4) is a transcription factor that plays key roles in stem cell self-renewal and tumorigenesis. However, its biological role and exact mechanism in cervical cancer remain unknown. Here, both immunohistochemistry (IHC) and Western blot assays demonstrated that the expression of PRDM4 in cervical cancer tissues was much lower than that in the normal cervix. A xenograft assay showed that PRDM4 overexpression in the cervical cancer cell lines SiHa and HeLa dramatically inhibited cell proliferation and tumorigenic potential in vivo. Conversely, the silencing of PRDM4 promoted cervical cancer cell proliferation and tumorigenic potential. Mechanistically, PRDM4 induced cell cycle arrest at the transition from G0/G1 phase to S phase by upregulating p27 and p21 expression and downregulating Cyclin D1 and CDK4 expression. Furthermore, the PI3K/AKT signaling pathway was inactivated in PRDM4-overexpressing cells, which decreased the levels of p-AKT and upregulated the expression of PTEN, an inhibitor of the PI3K/AKT signaling pathway, at both the transcriptional and translational levels. Dual-luciferase reporter assays and qChIP assays confirmed that PRDM4 transactivated the expression of PTEN by binding to two specific regions in the PTEN promoter. Furthermore, PTEN silencing or a PTEN inhibitor rescued the cell defects induced by PRDM4 overexpression. Therefore, our data suggest that PRDM4 inhibits cell proliferation and tumorigenesis by downregulating the activity of the PI3K/AKT signaling pathway by directly transactivating PTEN expression in cervical cancer.

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LETM1 Promotes Gastric Cancer Cell Proliferation, Migration, and Invasion via the PI3K/Akt Signaling Pathway

Journal of Gastric Cancer ◽

10.5230/jgc.2020.20.e12 ◽

2020 ◽

Vol 20 (2) ◽

pp. 139

Author(s):

Yunfeng Zhang ◽

Lele Chen ◽

Yifan Cao ◽

Si Chen ◽

Chao Xu ◽

...

Keyword(s):

Gastric Cancer ◽

Cell Proliferation ◽

Signaling Pathway ◽

Cancer Cell ◽

Gastric Cancer Cell ◽

Akt Signaling ◽

Migration And Invasion ◽

Cancer Cell Proliferation ◽

Akt Signaling Pathway ◽

Gastric Cancer Cell Proliferation

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LINC00511 is associated with the malignant status and promotes cell proliferation and motility in cervical cancer

Bioscience Reports ◽

10.1042/bsr20190903 ◽

2019 ◽

Vol 39 (9) ◽

Cited By ~ 7

Author(s):

Chun-Ling Yu ◽

Xiao-Ling Xu ◽

Fang Yuan

Keyword(s):

Cervical Cancer ◽

Cell Proliferation ◽

Cancer Patients ◽

Cancer Cell ◽

Clinical Stage ◽

Cervical Cancer Cell ◽

Migration And Invasion ◽

Epithelial Cell Line ◽

Cancer Cell Proliferation ◽

Poor Overall Survival

Abstract LINC00511 is a newly identified lncRNA that is up-regulated in many types of human cancers and may serve as an oncogenic lncRNA. However, there was no report about the role of LINC00511 in cervical cancer. Therefore, we investigated the clinical value of LINC00511 in cervical cancer patients via analyzing the correlation between LINC00511 expression and clinicopathological features. Moreover, we performed loss-of-function study to estimate the effect of LINC00511 on cervical cancer cell proliferation, migration, and invasion. In our study, we found LINC00511 expression levels were increased in cervical cancer tissues and cell lines compared with adjacent normal tissues and normal cervical epithelial cell line, respectively. High LINC00511 expression was correlated with advanced clinical stage, large tumor size, histological type of adenocarcinoma, and present lymph node metastasis, distant metastasis, and poor overall survival in cervical cancer patients. The in vitro studies indicated that knockdown of LINC00511 inhibited cervical cancer cell proliferation, migration, and invasion. In conclusion, LINC00511 acts as oncogenic lncRNA in cervical cancer, and may be a novel biomarker and potential therapeutic target for cervical cancer patients.

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