scholarly journals The effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats

2019 ◽  
Vol 12 (1) ◽  
pp. 115-127
Author(s):  
babisan askari ◽  
nahid bijeh ◽  
amir rashidlamir
Keyword(s):  
Resistance Training ◽  
Wistar Rats ◽  
P53 Expression ◽  
Male Wistar Rats ◽  
Cox 2

scholarly journals Synergistic Effect of Docetaxel Plus Saponin Fraction of Vitex doniana on Prostate Specific Antigen and p53 in Nitrsobis (2-oxopropyl) Amine-induced Prostate Toxicity

2021 ◽  
pp. 17-22
Author(s):  
Ifeanacho Ezeteonu Abireh ◽  
Godson Emeka Anyanwu
Keyword(s):  
Synergistic Effect ◽  
Prostate Specific Antigen ◽  
Wistar Rats ◽  
Specific Antigen ◽  
P53 Expression ◽  
Male Wistar Rats ◽  
Saponin Fraction ◽  
Vitex Doniana ◽  
Dose Dependent ◽  
Group 1

Aim: This study investigated the synergistic effect of docetaxel plus saponin fraction of Vitex doniana on prostate specific antigen and p53 in nitrsobis (2-oxopropyl) amine-induced prostate toxicity in Wistar rat. Methodology: Twenty-four (24) male Wistar rats with elevated serum prostate specific antigen level were selected from a group of sixty (60) rats pretreated with subcutaneous Nitrosobis (2-oxopropyl) amine 5 mg/kg daily for 4 weeks. The selected 24 male Wistar rats were then grouped into 6 groups of four (4) rats each. Group 1 was given 1ml normal saline daily from day 1-28. Groups 2, 3, 4, 5, and 6 further received subcutaneous nitrosobis (2-oxopropyl) amine 5 mg/kg daily from day 1-28. In addition, groups 3, 4, 5, and 6 were given weekly intravenous docetaxel 8 mg/kg on day 15 and 22. In addition to docetaxel, groups 4, 5, and 6 were further treated with oral saponin at 250 mg/kg, 500 mg/kg, and 750 mg/kg, respectively, daily, from day 15-28. Immunoenzymometric assay method was used for analysis of blood sample for prostate specific antigen. The prostate tissues were subjected to immuno study using the ImmunoCruz Staining System (Lab Vision Corporation, Fremont, CA, USA). The quantitative evaluation of p53 was done by calculating the percentages of p53-immunostained nuclei (labeling index). Results: Significant increase in prostate specific antigen and p53 expression were observed in group 2 (treated with Nitrsobis (2-oxopropyl) amine alone) when compared with group 1 (control). Dose dependent decrease in prostate specific antigen and p53 expression were observed in groups 4, 5, and 6, treated with docetaxel 8 mg/kg plus 250 mg/kg, 500 mg/kg, and 750 mg/kg of saponin respectively. Conclusion: Docetaxel plus Saponin fraction of Vitex doniana significantly reduced the serum prostate specific antigen concentration and p53 expression in a dose dependent manner, with the group treated with 750 mg/kg showing the highest decrease in the parameters tested.

scholarly journals Dehydration-induced increase in aquaporin-2 protein abundance is blocked by nonsteroidal anti-inflammatory drugs

2010 ◽  
Vol 298 (4) ◽  
pp. F1051-F1058 ◽  
Author(s):  
Erin Baggaley ◽  
Søren Nielsen ◽  
David Marples
Keyword(s):  
Wistar Rats ◽  
Hydration Status ◽  
Free Access ◽  
Protein Abundance ◽  
Aquaporin 2 ◽  
Male Wistar Rats ◽  
Access To Water ◽  
Inflammatory Drugs ◽  
Cox 2 ◽  
Selective Nsaid

It is now well established that the antidiuretic response to vasopressin is modulated by changes in aquaporin-2 (AQP2) expression in response to hydration status. While vasopressin itself is one signal driving expression, other signals also play a part. In this study, we planned to investigate whether prostaglandins, known to modulate AQP2 trafficking, may play a role in this process. Male Wistar rats were kept in metabolic cages, with either free access to water and food, or were given 15 g of food gelled with water, such that they were fluid restricted or fluid loaded. The effects of oral administration of two structurally different NSAIDs, indomethacin and ibuprofen, and a COX-2-selective NSAID, meloxicam, on urine output and AQP2 expression were investigated in kidneys removed under terminal anesthesia. All the NSAIDs decreased AQP2 expression significantly in water-restricted rats but did not significantly alter PGE excretion. In water-loaded rats, the effects were less marked, and meloxicam had no significant effect. Consistent with this, ibuprofen prevented the increase in AQP2 expression seen in response to dehydration. These results demonstrate that NSAIDs decrease AQP2 protein abundance, particularly during adaptation during dehydration. This may be of particular significance in older and critically ill patients, who are prone to dehydration.

Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats

2020 ◽  
Vol 235 (7-8) ◽  
pp. 5649-5665
Author(s):  
Rohollah Nikooie ◽  
Sohil Jafari‐Sardoie ◽  
Vahid Sheibani ◽  
Amir Nejadvaziri Chatroudi
Keyword(s):  
Resistance Training ◽  
Signaling Pathway ◽  
Wistar Rats ◽  
Muscle Hypertrophy ◽  
Smad Signaling ◽  
Smad Signaling Pathway ◽  
Male Wistar Rats ◽  
Tgf Β1

Enhanced Na+, K+-ATPase activity and endothelial modulation decrease phenylephrine-induced contraction in aorta from ouabain-treated normotensive and hypertensive rats

2014 ◽  
Vol 18 (2) ◽  
Author(s):  
Ana Paula Davel ◽  
Gisele Kruger Couto ◽  
Camilla Ferreira Wenceslau ◽  
Emilia Cristina Peres ◽  
Fabiano Elias Xavier ◽  
...  
Keyword(s):  
Protein Expression ◽  
Wistar Rats ◽  
Vascular Reactivity ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Vasoconstrictor Response ◽  
Adrenergic Response ◽  
Male Wistar Rats ◽  
Cox 2

AbstractThe purpose of this study was to compare the effect of long-term ouabain treatment on the vascular reactivity and NaMale Wistar rats were treated with ouabain (~8.0 µg/day, subcutaneously) or vehicle for 5 and 20 weeks, and spontaneously hypertensive rats (SHRs) for 5 weeks. Vasoconstrictor response to phenylephrine (10The phenylephrine-induced contraction was reduced, whereas the relaxation to KCl was enhanced in the aorta of ouabain-treated Wistar rats and SHRs. In both strains, endothelial modulation of α-adrenergic response was enhanced, related to an increased NO and reduced COX-derived vasoconstrictor factor modulation. Aortas from 20-week ouabain-treated Wistar rats showed reduced COX-2 and enhanced eNOS protein expression. In SHRs, 5-week ouabain treatment reduced COX-2 and increased nNOS protein expression.The results suggest that long-term ouabain treatment reduces the α-adrenergic response of aorta from normotensive rats and SHRs, associated with an increase of NO synthesis, reduced COX-2-derived vasoconstrictor factors, and enhanced ouabain-sensitive Na

scholarly journals Effects of Eight Weeks of Resistance Training on Muscle Myostatin Gene Expression and Insulin Resistance in Male Wistar Rats with Type 2 Diabetes

2019 ◽  
Vol 6 (4) ◽  
pp. 9-16
Author(s):  
Majid Hassan Qomi ◽  
Sajad Arshadi ◽  
Abdolali Banayifar ◽  
Yaser Kazemzadeh ◽  
◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Resistance Training ◽  
Wistar Rats ◽  
Myostatin Gene ◽  
Male Wistar Rats

scholarly journals 8 Weeks of Resistance Training Effect on Myostatin Gene Expression of Myocardium in Healthy Male Wistar Rats

2016 ◽  
Vol 22 (2) ◽  
pp. 111-116
Author(s):  
Amir Rashidlamir ◽  
Mohammad Reza Basami ◽  
Seyyed Reza Attarzadeh Hosseini ◽  
keyvan Hejazi ◽  
Seyyed Mohamad Motevalli Anberani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resistance Training ◽  
Wistar Rats ◽  
Training Effect ◽  
Healthy Male ◽  
Myostatin Gene ◽  
Male Wistar Rats
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