scholarly journals Effects of Eight Weeks of Resistance Training on Muscle Myostatin Gene Expression and Insulin Resistance in Male Wistar Rats with Type 2 Diabetes

2019 ◽  
Vol 6 (4) ◽  
pp. 9-16
Author(s):  
Majid Hassan Qomi ◽  
Sajad Arshadi ◽  
Abdolali Banayifar ◽  
Yaser Kazemzadeh ◽  
◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Resistance Training ◽  
Wistar Rats ◽  
Myostatin Gene ◽  
Male Wistar Rats

scholarly journals 8 Weeks of Resistance Training Effect on Myostatin Gene Expression of Myocardium in Healthy Male Wistar Rats

2016 ◽  
Vol 22 (2) ◽  
pp. 111-116
Author(s):  
Amir Rashidlamir ◽  
Mohammad Reza Basami ◽  
Seyyed Reza Attarzadeh Hosseini ◽  
keyvan Hejazi ◽  
Seyyed Mohamad Motevalli Anberani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resistance Training ◽  
Wistar Rats ◽  
Training Effect ◽  
Healthy Male ◽  
Myostatin Gene ◽  
Male Wistar Rats

scholarly journals Analysis of gene expression profiles in insulin-sensitive tissues from pre-diabetic and diabetic Zucker diabetic fatty rats

2005 ◽  
Vol 34 (2) ◽  
pp. 299-315 ◽  
Author(s):  
Young Ho Suh ◽  
Younyoung Kim ◽  
Jeong Hyun Bang ◽  
Kyoung Suk Choi ◽  
June Woo Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Zucker Diabetic Fatty Rats ◽  
Zdf Rats

Insulin resistance occurs early in the disease process, preceding the development of type 2 diabetes. Therefore, the identification of molecules that contribute to insulin resistance and leading up to type 2 diabetes is important to elucidate the molecular pathogenesis of the disease. To this end, we characterized gene expression profiles from insulin-sensitive tissues, including adipose tissue, skeletal muscle, and liver tissue of Zucker diabetic fatty (ZDF) rats, a well characterized type 2 diabetes animal model. Gene expression profiles from ZDF rats at 6 weeks (pre-diabetes), 12 weeks (diabetes), and 20 weeks (late-stage diabetes) were compared with age- and sex-matched Zucker lean control (ZLC) rats using 5000 cDNA chips. Differentially regulated genes demonstrating > 1.3-fold change at age were identified and categorized through hierarchical clustering analysis. Our results showed that while expression of lipolytic genes was elevated in adipose tissue of diabetic ZDF rats at 12 weeks of age, expression of lipogenic genes was decreased in liver but increased in skeletal muscle of 12 week old diabetic ZDF rats. These results suggest that impairment of hepatic lipogenesis accompanied with the reduced lipogenesis of adipose tissue may contribute to development of diabetes in ZDF rats by increasing lipogenesis in skeletal muscle. Moreover, expression of antioxidant defense genes was decreased in the liver of 12-week old diabetic ZDF rats as well as in the adipose tissue of ZDF rats both at 6 and 12 weeks of age. Cytochrome P450 (CYP) genes were also significantly reduced in 12 week old diabetic liver of ZDF rats. Genes involved in glucose utilization were downregulated in skeletal muscle of diabetic ZDF rats, and the hepatic gluconeogenic gene was upregulated in diabetic ZDF rats. Genes commonly expressed in all three tissue types were also observed. These profilings might provide better fundamental understanding of insulin resistance and development of type 2 diabetes.

scholarly journals Antioxidant status and hepato-protective role of Anchomanes difformis in streptozotocin-induced diabetes in male Wistar rats

2020 ◽  
Vol 66 (1) ◽  
pp. 18-36
Author(s):  
Toyin D. Alabi ◽  
Nicole L. Brooks ◽  
Oluwafemi O. Oguntibeju
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Liver Dysfunction ◽  
Antioxidant Status ◽  
Wistar Rats ◽  
Serum Levels ◽  
Male Wistar Rats ◽  
Hepatic Injuries

SummaryIntroduction: The liver is involved in the metabolism of xenobiotics and their metabolites and it is vulnerable to oxidative damage. Hyperglycaemia is highly implicated in the progression of diabetes mellitus, and adversely affects the liver. Though, conventional hypoglycaemic drugs may be effective in reducing blood glucose, they do not appear to be effective in attenuating the progression of diabetes and its complications.Objective: This study evaluated the ameliorative effects of Anchomanes difformis on hyperglycaemia and hepatic injuries in type 2 diabetes.Methods: Type 2 diabetes was induced in male Wistar rats with a single intraperitoneal injection of streptozotocin (40 mg/kg BW) after two weeks of fructose (10%) administration. Aqueous extract of A. difformis (200 and 400 mg/kg BW) and glibenclamide (5 mg/kg BW) were administered orally for six weeks. Blood glucose concentrations were measured. Serum levels of liver dysfunction markers (ALT, AST, and ALP), total cholesterol, triglycerides, HDL- and LDL-cholesterol were investigated. Total protein, albumin, and globulin were also assessed. Antioxidant parameters: ORAC, GSH, GSSG, SOD, CAT and FRAP were evaluated in the liver while ORAC, FRAP and lipid peroxidation were determined in the serum. Histological examination of the liver tissue was carried out.Results: Treatment with aqueous extract of A. difformis significantly (p<0.05) reduced blood glucose and reversed steatosis in the diabetic-treated rats. The antioxidant status of diabetic-treated rats was significantly (p<0.05) improved. Serum levels of liver dysfunction markers were significantly (p<0.05) reduced in diabetic-treated rats.Conclusion: The findings in this study revealed that 400 mg/kgBW Anchomanes difformis was more effective than 200 mg/kg BW in ameliorating diabetes-induced hepatopathy, however, both doses of Anchomanes difformis demonstrated more antidiabetic ability than glibenclamide. Anchomanes difformis may be a novel and potential therapeutic agent in the management of diabetes and resulted hepatic injuries.

scholarly journals Adipocyte PU.1 knockout promotes insulin sensitivity in HFD-fed obese mice

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Denise E. Lackey ◽  
Felipe C. G. Reis ◽  
Roi Isaac ◽  
Rizaldy C. Zapata ◽  
Dalila El Ouarrat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Insulin Sensitivity ◽  
Glucose Tolerance ◽  
Target Genes ◽  
Obese Mice ◽  
Tissue Macrophage

Abstract Insulin resistance is a key feature of obesity and type 2 diabetes. PU.1 is a master transcription factor predominantly expressed in macrophages but after HFD feeding PU.1 expression is also significantly increased in adipocytes. We generated adipocyte specific PU.1 knockout mice using adiponectin cre to investigate the role of PU.1 in adipocyte biology, insulin and glucose homeostasis. In HFD-fed obese mice systemic glucose tolerance and insulin sensitivity were improved in PU.1 AKO mice and clamp studies indicated improvements in both adipose and liver insulin sensitivity. At the level of adipose tissue, macrophage infiltration and inflammation was decreased and glucose uptake was increased in PU.1 AKO mice compared with controls. While PU.1 deletion in adipocytes did not affect the gene expression of PPARg itself, we observed increased expression of PPARg target genes in eWAT from HFD fed PU.1 AKO mice compared with controls. Furthermore, we observed decreased phosphorylation at serine 273 in PU.1 AKO mice compared with fl/fl controls, indicating that PPARg is more active when PU.1 expression is reduced in adipocytes. Therefore, in obesity the increased expression of PU.1 in adipocytes modifies the adipocyte PPARg cistrome resulting in impaired glucose tolerance and insulin sensitivity.

A gene expression signature for insulin resistance

2011 ◽  
Vol 43 (3) ◽  
pp. 110-120 ◽  
Author(s):  
Nicky Konstantopoulos ◽  
Victoria C. Foletta ◽  
David H. Segal ◽  
Katherine A. Shields ◽  
Andrew Sanigorski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Large Scale ◽  
Gene Expression Signature ◽  
Channel Blockers ◽  
Expression Signature ◽  
Insulin Resistant ◽  
Novel Strategy

Insulin resistance is a heterogeneous disorder caused by a range of genetic and environmental factors, and we hypothesize that its etiology varies considerably between individuals. This heterogeneity provides significant challenges to the development of effective therapeutic regimes for long-term management of type 2 diabetes. We describe a novel strategy, using large-scale gene expression profiling, to develop a gene expression signature (GES) that reflects the overall state of insulin resistance in cells and patients. The GES was developed from 3T3-L1 adipocytes that were made “insulin resistant” by treatment with tumor necrosis factor-α (TNF-α) and then reversed with aspirin and troglitazone (“resensitized”). The GES consisted of five genes whose expression levels best discriminated between the insulin-resistant and insulin-resensitized states. We then used this GES to screen a compound library for agents that affected the GES genes in 3T3-L1 adipocytes in a way that most closely resembled the changes seen when insulin resistance was successfully reversed with aspirin and troglitazone. This screen identified both known and new insulin-sensitizing compounds including nonsteroidal anti-inflammatory agents, β-adrenergic antagonists, β-lactams, and sodium channel blockers. We tested the biological relevance of this GES in participants in the San Antonio Family Heart Study ( n = 1,240) and showed that patients with the lowest GES scores were more insulin resistant (according to HOMA_IR and fasting plasma insulin levels; P < 0.001). These findings show that GES technology can be used for both the discovery of insulin-sensitizing compounds and the characterization of patients into subtypes of insulin resistance according to GES scores, opening the possibility of developing a personalized medicine approach to type 2 diabetes.

scholarly journals Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
O. E. Kale ◽  
O. B. Akinpelu ◽  
A. A. Bakare ◽  
F. O. Yusuf ◽  
R. Gomba ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Wistar Rats ◽  
High Fructose ◽  
Male Wistar Rats

scholarly journals The impact of circuit resistance training on serum glucose, insulin resistance and health related physical fitness in elderly men with type 2 diabetes

2020 ◽  
Vol 12 (3) ◽  
pp. 61-70
Author(s):  
HAMID ARAZI ◽  
ROGHAYEH GHOLIZADEH ◽  
AMIN SOHBATZADEH ◽  
EHSAN EGHBALI
Keyword(s):  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Resistance Training ◽  
Training Group ◽  
Serum Glucose ◽  
Fasting Serum ◽  
Elderly Men ◽  
Health Related ◽  
The Impact

Background: Obesity and decreased physical activity are the most important factors in the development of type 2 diabetes, which in recent decades has led to an increase in the number of people with this disease. The aim of this study was to investigate the impact of circuit resistance training (CRT) on serum glucose, insulin resistance and health related physical fitness in elderly men with type 2 diabetes. Material and methods: Twenty-two patients with type 2 diabetes (60.99 ±2.93 years) volunteered to participate in this study. They were divided randomly into two groups: training (n = 11) and control (n = 11). Participants in the training group performed a progressive CRT program for ten weeks. In addition, anthropometry variables, muscular strength and endurance were evaluated before and after ten weeks’ CRT. Also, 10 ml of the blood sample was taken from participants to measure fasting serum glucose, fasting serum insulin and insulin resistance. Results: After ten weeks of CRT, the body composition and glucose dropped significantly (P < 0.05) in the training group. Also, muscular endurance, upper and lower body strength in the post-test were significantly higher than the pre-test in the training group (P < 0.05). Conclusions: CRT led to a significant improvement in insulin resistance, fasting serum glucose, BMI, endurance and strength of elderly men with type 2 diabetes. Therefore, this type of resistance training can be useful for improvement in physical and physiological variables of elderly men with type 2 diabetes.

scholarly journals Effect of continuous aerobic training versus high intensity interval training on Resistin and insulin resistance in type 2 diabetic rats

2019 ◽  
Author(s):  
Shiva Ghafari ◽  
Parvaneh Nazarali ◽  
Ameneh Razavi ◽  
Maryam Delfan
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Wistar Rats ◽  
Aerobic Training ◽  
Interval Training ◽  
Maximum Speed ◽  
High Intensity Interval Training ◽  
High Intensity Interval ◽  
Type 2 Diabetic

Introduction: The adipose tissue produces and releases peptides that contributes to various processes in body, including insulin resistance. The aim of this study was to investigate the effect of eight weeks of continuous aerobic training versus high intensity interval training on Resistin and insulin levels and insulin resistance in type 2 diabetic male wistar rats. Methods: In this experimental research, twenty-four Wistar rats became diabetic in seven months. In next phase, after introducing the training environment, Wistar rats were randomly assigned into three equal groups of six each: control, continuous (20 minutes, 60% maximum speed) and intense interval (2 minutes of activity with 80% maximum speed, 2 minutes recovery with 30% maximum speed). The rats trained five times a week for eight weeks. Resistin gene expression and plasma insulin and glucose levels were measured before and after eight weeks. One-way ANOVA was carried out at P<0.05 for statistical analysis using SPSS software version16. Results: Regardless the type of training, differences between pre and post training results was statistically significant for insulin (P=0.024), glucose (P=0.037), insulin resistance (P=0.001) and Resistin (P=0.009). Interval training leads to the significant changes in all factors except the Resistin gene expression (P<0.05). There was a significant relationship between changes in insulin resistance and Resistin gene expression (P=0.005, r=0.63). Conclusion: The results of this study showed that training is an effective factor in insulin resistance process and related factors in diabetes, and Resistin also play a role in this process, but it seems that regular training is more important factor than its type to change the Expression of Resistin.

scholarly journals The Effects of Aerobic-Resistance Training and Broccoli Supplementation on Plasma Dectin-1 and Insulin Resistance in Males with Type 2 Diabetes

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3144
Author(s):  
Ayoub Saeidi ◽  
Mohammad Soltani ◽  
Ali Daraei ◽  
Hanieh Nohbaradar ◽  
Marjan Mosalman Haghighi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Resistance Training ◽  
Cardiometabolic Risk ◽  
Cardiometabolic Risk Factors ◽  
And Control

Background: This study aimed to evaluate the effects of a combination of aerobic-resistance training (CARET) and broccoli supplementation on dectin-1 levels and insulin resistance in men with type 2 diabetes mellitus (T2D). Methods: Forty-four males with T2D were randomly allocated to four groups (n = 11 each group): CARET + broccoli supplement (TS), CARET + placebo (TP), control + broccoli supplement (S), and control + placebo (CP). CARET was performed three days per week for 12 weeks. TS and S groups received 10 g of broccoli supplement per day for 12 weeks. All variables were assessed at baseline and 12 weeks. Results: Plasma dectin-1 levels were decreased in TS and TP groups compared with the CP group (p < 0.05). Cardiometabolic risk factors showed significant reductions in TP and TS groups compared to S and CP groups (p < 0.05). Conclusion: The combination of CARET and broccoli supplementation produced the largest improvements in insulin resistance and dectin-1 and other complications of T2D.

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