In Vitro Evaluation of Anti-Mitotic Activity of Green Synthesized Silver Nanoparticles from Ethanolic Extract of Chromolaena odorata, Caesalpinia coriaria (Bark and Leaves) using Allium cepa Roots

2021 ◽  
pp. 4107-4110
Author(s):  
Nishita Gogia ◽  
Anandhi D ◽  
Kanaga G ◽  
Revathi K
Keyword(s):  
Silver Nanoparticles ◽  
Mitotic Activity ◽  
Mitotic Index ◽  
Allium Cepa ◽  
Chromolaena Odorata ◽  
Root Tips ◽  
Antimitotic Activity ◽  
Biological Potential ◽  
Green Synthesized Silver Nanoparticles

The scope of the present study was to evaluate the In-vitro biological potential of Green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf). We investigated the effect of green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) by studying anti-mitotic activity An attempt has been made to evaluate the anti-mitotic activity of silver nanoparticles using Allium cepa roots. The mitotic index of the root tips meristem was calculated and compared with the standard methotrexate. Antimitotic activity results shows the mitotic index was ranged from 30-40%. Green synthesized silver nanoparticles from Chromolaena odorato, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) possess significant anti-mitotic activity.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

scholarly journals Cytogenotoxicity potentials of cocoa pod and bean-mediated green synthesized silver nanoparticles on Allium cepa cells

Caryologia ◽  
2017 ◽  
Vol 70 (4) ◽  
pp. 366-377 ◽  
Author(s):  
Taofeek A. Yekeen ◽  
Musibau A. Azeez ◽  
Agbaje Lateef ◽  
Tesleem B. Asafa ◽  
Iyabo C. Oladipo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Allium Cepa ◽  
Green Synthesized Silver Nanoparticles

scholarly journals Alterations in the mitotic index of Allium cepa induced by infusions of Pluchea sagittalis submitted to three different cultivation systems

2010 ◽  
Vol 82 (4) ◽  
pp. 857-860 ◽  
Author(s):  
Liana V. Rossato ◽  
Solange B. Tedesco ◽  
Haywood D. Laughinghouse IV ◽  
Júlia G. Farias ◽  
Fernando T. Nicoloso
Keyword(s):  
Mitotic Index ◽  
Allium Cepa ◽  
Antiproliferative Activity ◽  
Antiproliferative Effect ◽  
Growth Chamber ◽  
Cultivation System ◽  
Cultivation Systems ◽  
Onion Bulbs ◽  
Allium Cepa Test

We evaluated the antiproliferative effect of infusions from Pluchea sagittalis using the Allium cepa test. Infusions in three concentrations (2.5, 5, and 25 g dm-3) of leaves cultivated in three environments (in vitro, acclimatized growth chamber, and field) were used. Six onion bulbs were used for each of the eight treatments, and the mitotic index was obtained from 6000 cells per treatment. In conclusion, leaf infusions of P. sagittalis cultivated in the field have a high antiproliferative activity, as well as the cultivation system influences the antiproliferative potential.

In vitro and in vivo anti-inflammatory properties of green synthesized silver nanoparticles using Viburnum opulus L. fruits extract

2017 ◽  
Vol 79 ◽  
pp. 720-727 ◽  
Author(s):  
Bianca Moldovan ◽  
Luminita David ◽  
Adriana Vulcu ◽  
Liliana Olenic ◽  
Maria Perde-Schrepler ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Viburnum Opulus ◽  
Anti Inflammatory ◽  
Green Synthesized Silver Nanoparticles

scholarly journals Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) pulp extract having anticancer (in vitro) and antimicrobial applications

2019 ◽  
Vol 12 (8) ◽  
pp. 2572-2584 ◽  
Author(s):  
Md. Masud Rahaman Mollick ◽  
Dipak Rana ◽  
Sandeep Kumar Dash ◽  
Sourav Chattopadhyay ◽  
Biplab Bhowmick ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Abelmoschus Esculentus ◽  
Green Synthesized Silver Nanoparticles

DIURNAL RHYTHMS IN MITOSIS OF CELLS OF THE ONION ROOT TIP MERISTEM

1971 ◽  
Vol 13 (3) ◽  
pp. 597-599 ◽  
Author(s):  
Richard C. Bishop ◽  
Richard M. Klein
Keyword(s):  
Mitotic Activity ◽  
Allium Cepa ◽  
Constant Temperature ◽  
Time Of Day ◽  
Diurnal Rhythms ◽  
Root Tip ◽  
Root Tips ◽  
Complete Darkness ◽  
Onion Root

Seeds of Allium cepa were grown in complete darkness at constant temperature. The root tips, collected from hours 72 to 96 after the start of imbibition, exhibited four maxima and four minima in mitotic activity. The timing of this rhythm was dependent upon the time of imbibition and not upon the time of day.

scholarly journals In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures

2020 ◽  
Vol 9 (1) ◽  
pp. 538-553
Author(s):  
Saiqa Andleeb ◽  
Faiza Tariq ◽  
Areesha Muneer ◽  
Tooba Nazir ◽  
Beenish Shahid ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Protein Kinase ◽  
Brine Shrimp ◽  
Allium Sativum ◽  
Antibacterial Effect ◽  
Brine Shrimp Lethality ◽  
Prepared Nanoparticles ◽  
Protein Kinase Inhibition ◽  
Green Synthesized Silver Nanoparticles

AbstractThe current research aimed to evaluate in vitro biological activities of green-synthesized silver nanoparticles using the Allium sativum clove extract. The stability of green-synthesized silver nanoparticles was evaluated via storage at 4°C, room temperature (37°C), and calcined at 300°C, 500°C, and 700°C. The antibacterial effect was evaluated using agar well, spread plate, biofilm reduction, and cell proliferation inhibition assays. The cytotoxic and antidiabetic effects were determined via brine shrimp lethality, protein kinase inhibition, and α-amylase inhibition assays. DPPH scavenging, iron-chelating, anticoagulant, and hemolytic effects were evaluated. The highest inhibition of Klebsiella pneumoniae was observed when freshly prepared, calcined (300°C), and stored nanoparticles (4°C and 37°C) were applied (9.66, 9.55, 7.33, and 6.65 mm) against freshly prepared and calcined at 700°C which showed the highest inhibition of Pseudomonas aeruginosa (8.55 and 7.66 mm). Cell viability assay, biofilm reduction assay, and spread plate method showed a significant antibacterial effect of freshly prepared silver nanoparticles. Freshly prepared and calcined nanoparticles at 300°C and 500°C possessed strong antioxidant and iron-chelating activity. Among all the synthesized silver nanoparticles, freshly prepared and calcined nanoparticles (300°C and 500°C) increases the prothrombin time. Silver nanoparticles possessed significant anticoagulant properties and less toxic at least concentration toward human RBCs. In brine shrimp lethality assay, freshly prepared nanoparticles showed a stronger toxic effect and caused high mortality of larvae. Protein kinase inhibition assay revealed that freshly prepared nanoparticles had the highest zone of inhibition (18.0 mm) at 50 µg/disc. Green-synthesized nanoparticles would be used as potential therapeutic agents to overcome both infectious and noninfectious diseases.

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