The Specific Anti-cancerous Mechanisms Suggesting Spirulina Alga as a Promising breast Cancer Fighter

2021 ◽  
pp. 5599-5602
Author(s):  
Raghad J. Fayyad ◽  
Alaa Naseer Mohammed Ali ◽  
Noor T. Hamdan
Keyword(s):  
Breast Cancer ◽  
Standard Treatment ◽  
Cancer Cell Line ◽  
Antioxidant Property ◽  
Apoptosis Induction ◽  
Cellular Mechanisms ◽  
Lethal Challenge ◽  
Proliferative Effect ◽  
Current Review ◽  
Iraqi Women

Breast cancer is the most widely recognized and leading to global death incidences among women, traditional medications employ herbal sources in both prevention and dealing strategies of this lethal disease. It is well known that Patients suffering from cancer use plant derived therapy to complement or as alternatives for standard treatment .Strong anti-proliferative effects against cancer cell line by using Spirulina was explained previously by many researchers, thus, in current review authors elucidate the excite cellular mechanisms, involving with anti-proliferative effect of several constituents (phycocyanin, polysaccharides, flavonoids, resins, saponins and alkaloids) which have detected within this alga .these mechanisms include: inter action within the immune system, DNA repair, apoptosis induction as well as antioxidant property. The selection to focus on breast cancer treatment; is the widely spread of this type of cancer among Iraqi women through the last decades the current review give a possible and a promising insight of using this alga as a natural, cheap source in management of this lethal challenge.

Intronic miRNAs of Apoptosis Genes as Indicators of Cell Death

2019 ◽  
Vol 35 (6) ◽  
pp. 108-113
Author(s):  
J.A. Makarova ◽  
A.A. Poloznikov
Keyword(s):  
Breast Cancer ◽  
Cancer Cell Line ◽  
Ministry Of Education ◽  
Apoptosis Induction ◽  
Cell Models ◽  
Process Stage ◽  
Mature Micrornas ◽  
The Russian Federation ◽  
Apoptosis Level ◽  
Mcf 7

A method to assess the apoptosis level in cell models based on the analysis of the expression of micRNAs located in introns of apoptosis genes has been developed. Bioinformation analysis identified 536 genes associated with apoptosis; 30 of them contained 38 pre-microRNAs encoding 41 mature microRNAs. A significant change in the expression of hsa-miR-1244 and hsa-miR-4479 in response to apoptosis induction in the MCF-7 breast cancer cell line was revealed. A correlation was also found between the expression level of these miRNAs and the size of the primary tumor (process stage) in patients with breast cancer. apoptosis, microRNA, MCF7, breast cancer This work was supported by the Ministry of Education and Science of the Russian Federation (Project no. RFMEFI61618X0092).

Synthesis and In Vitro Anti-Breast Cancer Activity of Trametenolic Acid B Derivatives

2013 ◽  
Vol 634-638 ◽  
pp. 1135-1138 ◽  
Author(s):  
Ming Ruo Ding ◽  
Qiao Yin Zhang ◽  
Fang Hu ◽  
Nian Yu Huang ◽  
Jun Zhi Wang
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Elemental Analysis ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Cytotoxic Activities ◽  
Proliferative Effect ◽  
Cancer Activity

Six compounds were synthesized from the lanostane-type tetracyclic triterpenoid of trametenolic acid B with the aim of obtaining more active compounds, which were characterized by NMR, IR, ESI-MS and elemental analysis. Their cytotoxic activities were evaluated against the breast cancer cell line MD-AMB-231. The compound 3b exhibited the best anti-proliferative effect than trametenolic acid B and other derivatives with the IC50 of 17.78 µM.

scholarly journals The selective anti-proliferative and pro-apoptotic effect of A. cherimola on MDA-MB-231 breast cancer cell line

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Maria Younes ◽  
Carl Ammoury ◽  
Tony Haykal ◽  
Leah Nasr ◽  
Rita Sarkis ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Annexin V ◽  
Proliferative Effect ◽  
Apoptotic Effect

Abstract Background Herbal medicines have been a major target for numerous studies through the past years as an alternative treatment for cancer, mainly due to their minimal effects on normal healthy cells. Annona cherimola, popularly known as Cherimoya, is an edible natural fruit rich in phytochemical components and known to possess various biological activities. Previous studies have reported the anti-cancerous effect of A. cherimola ethanolic leaf extract (AELE) on leukemia. This study aims at studying the potential anti-cancer activity of this extract in vitro in two different breast cancer cell lines, namely MDA-MB-231 and MCF-7, in addition to investigating its toxicity on normal mesenchymal stem cells. Methods The anti-proliferative effect of AELE was evaluated via cell viability assay. Propidium iodide staining, Cell Death Detection ELISA and flow cytometry analysis of Annexin V binding were used to assess cell cycle progression, DNA fragmentation and apoptosis induction, respectively. Protein expression was determined via Western Blot analysis to decipher the underlying apoptotic molecular mechanism induced upon AELE treatment. Results The anti-proliferative effect of the extract was found to be selective on the triple-negative breast cancer cell line (MDA-MB-231) in a time- and dose-dependent manner with an IC50 of 390.2 μg/mL at 48 h, with no cytotoxic effects on normal murine mesenchymal stem cells. The pro-apoptotic effect was confirmed by the increase in cellular and DNA fragmentation, flipping of the phosphatidylserine moiety to the outer leaflet, and the increase in Annexin V binding. The underlying molecular mechanism revealed the involvement of the mitochondrial pathway, as shown by alterations in mitochondrial permeability and the upregulation of cytochrome c expression. Conclusion All the data presented in our study suggest that AELE exhibits a selective anti-proliferative and pro-apoptotic effect on the chemo-resistant MDA-MB-231 breast cancer cells, providing evidence for the anti-tumor effects of A. cherimola.

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