Basic Concepts of Sterilization Techniques

2021 ◽  
pp. 155-161
Author(s):  
Ravi Kumar ◽  
Atul Rana
Keyword(s):  
Chemical Agent ◽  
Infectious Agents ◽  
Wet Steam ◽  
Complete Destruction ◽  
Chemical Sterilization ◽  
Basic Concepts ◽  
Forms Of Life

Sterilization, which is any process, physical or chemical, that destroys all forms of life, it is used especially to destroy microorganisms, spores, and viruses. Precisely defined, sterilization is the complete destruction of all microorganisms by a suitable chemical agent or by heat, either wet steam. In this review, we discussed about various suitable techniques that used for removing of infectious agents. The heat sterilization can be applied only to the thermostable products, and chemical sterilization is also used for any types of plastic or glass materials that degrade with heat. The Gas sterilization involves exposing equipment to chemical gases in an enclosed heated or pressurized chamber.

scholarly journals Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Natália Helena Gavilan ◽  
Fernanda Cardoso Furlan ◽  
Alex Zichner Zorz ◽  
Leandro Silva de Oliveira ◽  
Wellington Ferreira Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Culture Medium ◽  
Genetic Erosion ◽  
Chemical Agent ◽  
Shoot Induction ◽  
Popular Medicine ◽  
In Vitro Multiplication ◽  
Apical Buds ◽  
Chemical Sterilization

ABSTRACT: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.

scholarly journals Benzalkonium Chloride Sterilization of Nonwoven Fibrous Scaffolds for Astrocyte Culture

2009 ◽  
Vol 3 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Robin Ng ◽  
Jesse Singh Gurm ◽  
Shang-Tian Yang
Keyword(s):  
Tissue Engineering ◽  
Polylactic Acid ◽  
Benzalkonium Chloride ◽  
Quaternary Salt ◽  
Tissue Scaffolds ◽  
Chemical Agent ◽  
Bacterial Contaminants ◽  
Pet Fibers ◽  
Chemical Sterilization ◽  
Fibrous Matrices

Tissue engineering is an emerging field in biomedicine, holding enormous promise for regenerative medicine. Scaffolds, within which cells proliferate, are a controlling factor in tissue engineering applications. Upon fabrication, tissue scaffolds must undergo appropriate sterilization to eliminate contaminants. Current sterilization methods are either costly, time consuming, or ineffective. In this study, a quaternary salt, benzalkonium chloride (BAC), was used as a chemical agent for sterilization of nonwoven polyethylene terephthalate (PET) fibers and polylactic acid nanofibers. Treating the PET scaffolds with 0.1% (w/v) BAC for only 2 minutes was effective to eliminate bacterial contaminants in the fibrous matrices. In addition, astrocyte cells were successfully cultured in the PET scaffolds following BAC sterilization, demonstrating the suitability of BAC as a sterilization agent. This chemical sterilization method is also mild and nonabrasive to nanostructured materials such as electrospun polylactic acid nanofibers.

Pathology of Bone Cells in Pigs with Experimental Turbinate Osteoporosis (Atrophic Rhinitis)

1971 ◽  
Vol 29 ◽  
pp. 304-305
Author(s):  
A. W. Fetter ◽  
C. C. Capen
Keyword(s):  
Mucous Membrane ◽  
Bone Cells ◽  
Atrophic Rhinitis ◽  
Infectious Agents ◽  
Metabolic Disturbances ◽  
Progressive Reduction ◽  
Naturally Occurring ◽  
Fundamental Cause ◽  
Osteocytic Osteolysis ◽  
Uncertain Etiology

Atrophic rhinitis in swine is a disease of uncertain etiology in which infectious agents, hereditary predisposition, and metabolic disturbances have been reported to be of primary etiologic importance. It shares many similarities, both clinically and pathologically, with ozena in man. The disease is characterized by deformity and reduction in volume of the nasal turbinates. The fundamental cause for the localized lesion of bone in the nasal turbinates has not been established. Reduced osteogenesis, increased resorption related to inflammation of the nasal mucous membrane, and excessive resorption due to osteocytic osteolysis stimulated by hyperparathyroidism have been suggested as possible pathogenetic mechanisms.The objectives of this investigation were to evaluate ultrastructurally bone cells in the nasal turbinates of pigs with experimentally induced atrophic rhinitis, and to compare these findings to those in control pigs of the same age and pigs with the naturally occurring disease, in order to define the fundamental lesion responsible for the progressive reduction in volume of the osseous core.

Atom-probe analysis of the solid-liquid interface

1995 ◽  
Vol 53 ◽  
pp. 676-677
Author(s):  
J.A. Panitz
Keyword(s):  
Molecular Level ◽  
Selective Adsorption ◽  
Electronic Devices ◽  
Atom Probe ◽  
Chemical Agent ◽  
Hostile Environment ◽  
Solid Interface ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Chemically Selective

The first few atomic layers of a solid can form a barrier between its interior and an often hostile environment. Although adsorption at the vacuum-solid interface has been studied in great detail, little is known about adsorption at the liquid-solid interface. Adsorption at a liquid-solid interface is of intrinsic interest, and is of technological importance because it provides a way to coat a surface with monolayer or multilayer structures. A pinhole free monolayer (with a reasonable dielectric constant) could lead to the development of nanoscale capacitors with unique characteristics and lithographic resists that surpass the resolution of their conventional counterparts. Chemically selective adsorption is of particular interest because it can be used to passivate a surface from external modification or change the wear and the lubrication properties of a surface to reflect new and useful properties. Immunochemical adsorption could be used to fabricate novel molecular electronic devices or to construct small, “smart”, unobtrusive sensors with the potential to detect a wide variety of preselected species at the molecular level. These might include a particular carcinogen in the environment, a specific type of explosive, a chemical agent, a virus, or even a tumor in the human body.

A Cognitive-Linguistic Intervention Program: Basic Concept Formation Level

1977 ◽  
Vol 8 (1) ◽  
pp. 23-32
Author(s):  
Gerald E. Chappell
Keyword(s):  
Basic Concept ◽  
Concept Formation ◽  
Intervention Program ◽  
Cognitive Linguistic ◽  
Basic Concepts ◽  
Linguistic Skills ◽  
Linguistic Intervention

Test-teach questioning is a strategy that can be used to help children develop basic concepts. It fosters the use of multisensory exploration and discovery in learning which leads to the development of cognitive-linguistic skills. This article outlines some of the theoretical bases for this approach and indicates possibilities for their applications in child-clinician transactions.

Prekindergarteners' Performance on The Boehm Test of Basic Concepts

1988 ◽  
Vol 16 ◽  
pp. 137-141 ◽  
Author(s):  
Amelia Ouellette ◽  
Robert Casteel
Keyword(s):  
Basic Concepts

The Developing Relationship Among Cognition, Amplification, and Aural Rehabilitation

2016 ◽  
Vol 1 (6) ◽  
pp. 47-54 ◽  
Author(s):  
Jeffrey J. DiGiovanni ◽  
Travis L. Riffle
Keyword(s):  
Working Memory ◽  
Best Practices ◽  
Cognitive Abilities ◽  
Hearing Aid ◽  
Hearing Aid Fitting ◽  
Aural Rehabilitation ◽  
Basic Concepts ◽  
And Function

The search for best practices in hearing aid fittings and aural rehabilitation has generally used the audiogram and function stemming from peripheral sensitivity. In recent years, however, we have learned that individuals respond differently to various hearing aid and aural rehabilitation techniques based on cognitive abilities. In this paper, we review basic concepts of working memory and the literature driving our knowledge in newer concepts of hearing aid fitting and aural rehabilitation.

The Boehm Test of Basic Concepts

1973 ◽  
Vol 6 (1) ◽  
pp. 63-65 ◽  
Author(s):  
Theodore A. Dahl
Keyword(s):  
Basic Concepts
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