High Expression of sLex Associated with Poor Survival in Argentinian Colorectal Cancer Patients

2014 ◽  
Vol 29 (1) ◽  
pp. e30-e39 ◽  
Author(s):  
Ariel Zwenger ◽  
Martin Rabassa ◽  
Sandra Demichelis ◽  
Gabriel Grossman ◽  
Amada Segal-Eiras ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Normal Mucosa ◽  
Poor Survival ◽  
Primary Tumors ◽  
Metastatic Lymph Nodes ◽  
Metastatic Lymph ◽  
Colorectal Cancer Patients

Aim Colorectal cancer (CRC) is one of the most prevalent malignancies in Argentina with 11,043 new cases and 6,596 deaths estimated to have occurred in 2008. The present study was developed to clarify the differential expression of MUC1, MUC2, sLex, and sLea in colorectal cancer patients and their relationship with survival and clinical and histological features. Methods Ninety primary tumor samples and 43 metastatic lymph nodes from CRC patients were studied; follow-up was documented. Twenty-six adenoma and 68 histological normal mucosa specimens were analyzed. An immunohistochemical approach was applied and statistical analysis was performed. Results In tumor samples, MUC1, sLea, and sLex were highly expressed (94%, 67%, and 91%, respectively); also, we found a significantly increased expression of the 3 antigens in primary tumors and metastatic lymph nodes compared with normal mucosa and adenomas. MUC2 was expressed in 52% of both normal mucosa and CRC samples; this reactivity significantly decreased in metastatic lymph nodes (p<0.05). A multiple comparison analysis showed that MUC1 and sLex discriminated among 3 groups: normal, adenoma, and CRC tissues. The increase of sLex expression showed an association with recurrence, and survival analysis showed that a high sLex staining was significantly associated with a poor survival. By multivariate analysis MUC1 inmunoreactivity correlated positively and significantly with tumor size, while MUC2 expression showed the opposite correlation. Conclusions The correlation of sLex overexpression in primary tumors and metastatic lymph nodes, the discrimination among the normal, adenoma, and CRC groups based on sLex expression, as well as its association with recurrence and survival, all suggest a prognostic role of sLex in Argentinian CRC patients.

RA07.09: THE EFFICACY OF ASSESSING METASTATIC LYMPH NODES BY COMPUTED TOMOGRAPHY IN ADVANCED ESOPHAGEAL CANCER WITH NEOADJUVANT CHEMOTHERAPY

2018 ◽  
Vol 31 (Supplement_1) ◽  
pp. 37-37
Author(s):  
Shinya Urakawa ◽  
Tomoki Makino ◽  
Koji Tanaka ◽  
Yasuhiro Miyazaki ◽  
Tsuyoshi Takahashi ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Neoadjuvant Chemotherapy ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Reduction Rate ◽  
Short Axis ◽  
Advanced Esophageal Cancer ◽  
Primary Tumors ◽  
Metastatic Lymph Nodes ◽  
Metastatic Lymph

Abstract Background Although various modalities including CT, endoscopy, and positron emission tomography (PET) have been used to predict pathological tumor response (pTR) to neoadjuvant chemotherapy (NACT) or prognosis in esophageal cancer patients, an optimal method of response evaluation remains to be established. Methods A total of 97 non-T4 thoracic esophageal cancer patients who underwent curative surgery after NACT from 2011 to 2014 with both measurable primary tumors (PT) and metastatic lymph nodes (LNs) by CT (≥ 10mm in short axis or ≥ 5mm in short axis with SUV-max ≥ 2.5) were analyzed. Patients with ≥ 50% decrease in size of the PT (two-direction measurement) and ≥ 30% decrease in size of LNs (sum of short diameter based on RECIST criteria) were defined as PT- and LNs-responders, respectively. Results The median reduction rate of PT and LNs were 62.0% (5.5–93.4) and 26.7% (60.9–13.6), respectively. Of 97 patients, 62 (69%) and 45 patients (45%) were classified as PT- and LNs-responders respectively. The concordance rate between CT response of PT and LNs was 66% (P = 0.01). CT response of PT was correlated with pTR (P<0.0001) while CT response of LNs was associated with pT (P = 0.0011), pN(P = 0.0004), and pTR (P < 0.0001). Receiver operating characteristic (ROC) curves demonstrated the highest accuracy (AUC 0.75), sensitivity (88.9%) and specificity (60.9%) at the reduction rate of 53% (P = 0.007) which was approximated to the cutoff value we used. In univariate analysis of recurrence free survival (RFS), pT, pN, pM, CT response of both PT and LNs, and pTR were significantly correlated with RFS. Multivariate analysis further identified CT response of LNs (HR 2.68 P = 0.003) and pTR (HR 2.72 P = 0.022) to be independently associated with RFS. When classified into three groups by histological grade and CT response of LNs [group A (grade2–3/LNs-responders), group B (grade1a-1b/LNs-nonresponders), and group C (others)], 2-year RFS were 83% in the groupA, 59% in groupC and 29% in groupB (P < 0.0001), respectively. Conclusion In locally-advanced esophageal cancer patients with NACT followed by surgery, CT response of PT and LNs significantly correlated with pTR. Especially, CT response of LNs was important to predict prognosis in addition to pTR. Disclosure All authors have declared no conflicts of interest.

Better retrieval of lymph nodes in colorectal resection specimens by pathologists’ assistants

2012 ◽  
Vol 66 (1) ◽  
pp. 18-23 ◽  
Author(s):  
C C H J Kuijpers ◽  
H J van Slooten ◽  
W H Schreurs ◽  
G R H M Moormann ◽  
M A Abtahi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Patient Safety ◽  
Adjuvant Chemotherapy ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Colorectal Resection ◽  
Number Of Patients ◽  
Pathology Reports ◽  
Colorectal Cancer Patients

BackgroundErrors in surgical pathology are partly due to the increasing workload of pathologists. To reduce this workload, ‘pathologists’ assistants’ (PAs) have been trained to take over some of the pathologists’ recurrent tasks. One of these tasks is the precise examination of ≥10 lymph nodes (LNs), which is of paramount importance to reduce the risk of understaging of colorectal cancer patients.AimsTo evaluate the role of PAs in harvesting LNs in colorectal resection specimens and, by doing so, in improving patient safety.MethodsLN harvest was retrospectively reviewed in 557 pathology reports on colorectal resection specimens collected in two Dutch hospitals from 2008 until 2011.ResultsPAs sampled ≥10 LNs in significantly more cases than pathologists did (83.2% vs 60.9% in hospital A and 79.2% vs 67.6% in hospital B) and recovered on average significantly more LNs than pathologists did (18.5 vs 12.2 in hospital A and 16.6 vs 13.2 in hospital B). PAs harvested a significantly higher percentage of LNs <5 mm than pathologists did (64.2% vs 53.7%). The percentages of colon cancer patients eligible for adjuvant chemotherapy due to inadequate LN sampling alone were significantly higher for cases dissected by pathologists than for those dissected by PAs (17.3% vs 1.1% in hospital A and 13.1% vs 3.4% in hospital B)ConclusionsPAs contribute to patient safety since they recover more and, in particular, smaller LNs from colorectal resection specimens than pathologists do. Moreover, they help to reduce costs and morbidity by reducing the number of patients eligible for adjuvant chemotherapy due to inadequate LN sampling alone.

Evaluation of Serum Carcinoembryonic Antigen Monitoring in the follow-up of Colorectal Cancer Patients with Metastatic Lymph Nodes and a Normal Preoperative Serum Level

1997 ◽  
Vol 12 (1) ◽  
pp. 18-21 ◽  
Author(s):  
E. Tobaruela ◽  
J.M. Enriquez ◽  
M. Diez ◽  
J. Camunas ◽  
J. Muguerza ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Lymph Nodes ◽  
Carcinoembryonic Antigen ◽  
Cancer Patients ◽  
Predictive Value ◽  
Hepatic Metastases ◽  
Metastatic Lymph Nodes ◽  
Serum Carcinoembryonic Antigen ◽  
Colorectal Cancer Patients

The value of serial serum carcinoembryonic antigen (CEA) assay in the follow-up of colorectal cancer patients with metastatic lymph nodes and normal (≤ 5 ng/ml) preoperative CEA levels, was examined in this study. Thirty-eight patients were studied and compared with 22 patients with elevated CEA levels. The overall sensitivity of CEA for the diagnosis of recurrence was 36%. Postoperative CEA was strongly influenced by the site of recurrence. CEA monitoring showed the best results in patients who developed hepatic metastases (sensitivity 60%, specificity 94%, positive predictive value 60%, and negative predictive value 94%), and was ineffective for the detection of locoregional or pulmonary metastases. The results indicate that elevation of CEA in the postoperative course of these patients is an indicator of the presence of hepatic metastases. Postoperative CEA monitoring should not be omitted in Dukes C patients with normal preoperative levels, and is more reliable for the detection of liver metastases.

R425 – Association of CD44 v6 and v10 Isoforms In HNSCC Progression

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P186-P186
Author(s):  
Steven J Wang ◽  
Anne-Martine De Heer
Keyword(s):  
Lymph Nodes ◽  
Cell Lines ◽  
Immunohistochemical Analysis ◽  
Cell Growth And Migration ◽  
Primary Tumors ◽  
Metastatic Lymph Nodes ◽  
Variant Isoforms ◽  
Metastatic Lymph ◽  
Hnscc Cell

Problem The CD44 family of receptors includes multiple variant isoforms, several of which have been linked to tumor progression. The objective of this study was to investigate the role of CD44 v6- and v10-containing isoforms in head and neck squamous cell carcinoma (HNSCC) progression. Methods A laboratory investigation utilizing a panel of 13 HNSCC cell lines and clinical tissue specimens from a series of 82 patients with HNSCC was performed. Results Immunohistochemical analysis of 82 primary HNSCC tumors and 24 metastatic lymph nodes revealed expression of CD44 v6 and v10 variants. A greater proportion of metastatic lymph nodes vs. primary tumors demonstrated strong expression of CD44 v6 (18/24 vs. 26/82) and CD44 v10 (14/24 vs. 16/82), while expression of standard CD44 was not significantly different in metastatic lymph nodes and primary tumors (10/24 vs 60/82). In addition, greater than expected expression of CD44 v10 was found among 14 specimens obtained from patients previously treated with chemoradiation. To further analyze the role of these CD44 variants in HNSCC progression, HNSCC cell lines were selected on the basis of their CD44 v6 and v10 expression. A HNSCC cell line without CD44 expression and cells of the same variant-expressing cell lines transfected with short-hairpin RNA for CD44 were used as controls. Compared to controls, HNSCC cells expressing CD44 v6-containing isoforms demonstrated increased hyaluronan-mediated tumor cell growth and migration. Furthermore, HNSCC cells expressing CD44 v10-containing isoforms demonstrated increased hyaluronan-mediated cisplatin resistance. Conclusion CD44 v6- and v10-containing isoforms are associated with HNSCC progression. Significance CD44 v6- and v10-containing isoforms may be useful tumor markers and targets for HNSCC therapy. Support This work was supported by an AAO-HNS/AHNS Young Investigator Award.

scholarly journals Is the preservation of the left colic artery an ideal choice for colorectal cancer surgery? A meta-analysis

2020 ◽  
Author(s):  
Ruize Qu ◽  
Fei Li ◽  
Bingyan Wang ◽  
Siyi Lu ◽  
Junren Ma ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Rectal Cancer ◽  
Lymph Nodes ◽  
Anastomotic Leakage ◽  
Cancer Patients ◽  
Operation Time ◽  
Metastatic Lymph Nodes ◽  
Metastatic Lymph ◽  
Significant Difference ◽  
Registration Number

Abstract Background: Left colic artery (LCA) preservation or non-preservation in radical resection for colorectal cancer is still under debate. This study aimed to compare the perioperative and oncological outcomes between the two procedures.Methods: Systematic search was performed in PubMed, Medline, Embase, Web of Science, and China National Knowledge Infrastructure databases for relevant randomized and non-randomized clinical trials published between 2011 and 2019. The primary endpoints were 5-year overall survival (OS), 5-year disease-free survival (DFS), total lymph nodes harvested, and anastomotic leakage. Secondary endpoints included the number of metastatic lymph nodes, intraoperative blood loss, urinary dysfunction, bowel obstruction, and operation time. Results: Twenty-eight studies with 10545 patients (LCA non-preservation surgery, 4920; LCA preservation surgery, 5625) were included. Data of 7142 rectal cancer patients (LCA non-preservation surgery, 3468; LCA preservation surgery, 3674) were extracted for subgroup analysis. There was significantly lower incidence of anastomotic leakage (odds ratio=1.21; 95% confidence interval |1.04, 1.41|; P=0.015) in colorectal cancer patients with LCA preservation. When rectal cancer was independently analyzed, no significant difference was found in anastomotic leakage between the groups. There were significantly more metastatic lymph nodes and significantly shorter operation time in colorectal cancer and rectal cancer patients with LCA non-preservation. No significant difference was found regarding 5-year OS, 5-year DFS, total lymph nodes harvested, intraoperative blood loss, urinary dysfunction, and bowel obstruction for colorectal and rectal cancer.Conclusions: LCA non-preservation was not proved to increase anastomotic leakage in rectal cancer surgery and was associated with more harvested metastatic lymph nodes and shorter operation time. Trial registration: A review protocol was registered on PROSPERO (registration number: CRD42020183906) and http://www.researchregistry.com (registration number: reviewregistry841).

Phosphorylated Akt and MAPK expression in primary tumors and in corresponding metastases and clinical outcome in colorectal cancer patients receiving irinotecan-cetuximab.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e14086-e14086
Author(s):  
Riccardo Giampieri ◽  
Mario Scartozzi ◽  
Elena Maccaroni ◽  
Alessandra Mandolesi ◽  
Simona Biagetti ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Cancer Patients ◽  
Progression Free Survival ◽  
Primary Tumors ◽  
Kaplan Meier ◽  
Phosphorylated Akt ◽  
Line Setting ◽  
Colorectal Cancer Patients

e14086 Background: Other than the well-known EGFR pathway activation via the Ras-Raf-MAP-kinase, a possible role of other potential biomarkers could be relevant in determining resistance to anti-EGFR treatment, such as the protein-serine/threonine kinase Akt. We tried to assess the role of Akt and MAPK expression in metastatic colorectal cancer patients and their relationship with outcome for patients receiving Irinotecan-Cetuximab. Methods: Eligible patients were metastatic colorectal cancer patients treated in 2nd or 3rd line setting with a irinotecan-cetuximab based regimen. Patients were tested for K-ras status and subsequently assessed by immunoistochemistry for pAkt and MAPK expression, both in primary tumours and metastases whenever sufficient tissue was available. The role of pAkt and MAPK expression was evaluated for K-ras wild type patients for different likelihood of response, overall survival and progression free survival. Response was evaluated by RECIST criteria. Survival analysis was performed via Kaplan-Meier method. Results: In metastases pAkt correlated with RR (9% vs. 58%, p=0.004), PFS (2.3 months vs.9.2 months p < 0.0001) and OS (6.1 months vs.26.7 months p < 0.0001) and pMAPK correlated with RR (10% vs, 47%, p = 0.002), PFS (2.3 months vs.8.6 months p < 0.0001) and OS (7.8 months vs.26 months p=0.0004). At multivariate analysis pAkt and pMAPK in metastases were able to independently predict PFS. pAkt in metastases independently correlated with RR as well. Conclusions: pAkt and pMAPK expression in metastases may modulate the activity of EGFR-targeted antibodies. We could speculate that in patients with pAkt and pMAPK metastases expression targeting these factors may be crucial.

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