scholarly journals Production of New Regenerated Plant by Anther Culture of Italian Ryegrass

2002 ◽  
Vol 22 (3) ◽  
pp. 209-212 ◽  
1993 ◽  
Vol 73 (3) ◽  
pp. 777-783 ◽  
Author(s):  
P. Masojc ◽  
O. M. Lukow ◽  
R. I. H. McKenzie ◽  
N. K. Howes

Anther culturability of 43 cultivars and 6 F1 crosses representing different quality classes of spring wheat was studied using a glucose-containing, modified N6 medium (HNG). Generally high pollen embryoid formation (up to 111 embryoids per 100 anthers) was associated with lower green plant regeneration (up to 9.1 green plantlets per 100 embryoids) frequencies and a high proportion (63% on average) of albino plants. Anther response was found to be strongly affected by the genotype of the donor plants. Seven of the screened cultivars yielded more than one green regenerated plant per 100 anthers. The most responsive cultivars were Veery #2 (6.2), ST 6 (4.0), and Leader (3.6). No ability to regenerate green plantlets was shown by 10 of the genotypes. Anther responsiveness of F1 progenies as compared with the parental cultivars were different in each cross. Differences found between reciprocal crosses suggest that the cytoplasm of a donor plant may affect anther response. Haploid plants constituted 45% of the anther derived regenerants, while the remaining part was divided into 29% of spontaneous diploids and 26% of plants with abnormal chromosome numbers. Key words: Triticum aestivum, anther culture, doubled haploids, wheat


2002 ◽  
Vol 22 (4) ◽  
pp. 273-278 ◽  
Author(s):  
K.Y. Kim ◽  
K.M. Kang ◽  
K.J. Choi ◽  
Y.S. Jang ◽  
Y.C. Lim ◽  
...  

Author(s):  
R.J. Densley ◽  
G.M. Austin ◽  
I.D. Williams ◽  
R. Tsimba ◽  
G.O. Edmeades

Trade-offs in dry matter (DM) and metabolisable energy (ME) between combinations of three maize silage hybrids varying in maturity from 100-113 CRM and six winter forage options were investigated in a Waikato farmer's field over 2 years. Winter crops were triticale, cut once; oats grazed 1-2 times; and Tama and Feast II Italian ryegrass, each cut or grazed 2-3 times. Greatest DM and ME production (38.9 t/ha; 396 GJ/ha) was from a 113 CRM hybrid followed by a single-cut triticale crop. The most economical sources of DM and ME were obtained from a 100 CRM maize hybrid plus grazed oats (11.8 c/ kg; 1.12 c/MJ), while the cheapest ME source among cut winter forages was a 113 CRM maize hybrid + triticale (1.18 c/MJ). Reliable annual silage production of 30 t DM/ha and 330 GJ ME/ha (or 3000 kg MS/ha) is possible using a late maturing maize hybrid combined with a winter forage crop such as triticale, although the low feed value of the triticale may limit its use as feed for milking cows. Keywords: Italian ryegrass, oats, maize silage, supplements, triticale, winter forage crops


2018 ◽  
Vol 44 (2) ◽  
pp. 219-232 ◽  
Author(s):  
S.J Jang ◽  
K.R. Kim ◽  
Y.B. Yun ◽  
S.S. Kim ◽  
Y.I Kuk

2018 ◽  
Vol 45 (4) ◽  
pp. 776-793
Author(s):  
Hyunho Choi ◽  
Jinwoo Yun ◽  
Yongwoo Chae ◽  
Jeahyoung Park
Keyword(s):  

Growth regulators, phytohormones, both natural and artificial, are the main means to control plant ontogenesis. They are involved in regulating the processes of cell differentiation and cell divisions, the formation of tissues and organs, the changes in the rate of growth and development, the duration of the certain stages of ontogenesis. The main classes of phytohormones used in plant biotechnology, in particular, in the induction of haploid structures, are auxins and cytokinins. The mechanism of action of phytohormones on a cell is rather complicated and may have a different character. Understanding the characteristics of the action of phytohormones is complicated by the fact that the system of hormonal regulation of plant life is multicomponent. This is manifested in the fact that the same physiological process is most often influenced not by one, but by several phytohormones, covering a wide range of aspects of cell metabolism. In connection with the foregoing, the purpose of our work was to test a set of nutrient media with different basic composition and different proportions of phytohormones to determine the patterns of their influence on the processes of haploid structure induction in rape anther culture using accessions, developed at the Institute of Oilseed Crops NAAS. The material used was two accessions of winter rapeseed (No. 1 and No. 2) and one sample of spring rapeseed, provided by the Rapeseed Breeding laboratory of the Institute of Oilseed Crops. Incised inflorescences were kept against the background of low temperature of 6–8 ° C for several days, and then, under aseptic conditions, anthers with unripe pollen grains were isolated and planted on nutrient media differing in both basic mineral composition and content of phytohormones. MS (Murashige & Skoog 1962) and B5 (Gamborg et al 1968) media were used as basic media. Phytohormones were added to the basic media in various combinations – BA, 2,4-D, NAA at the concentrations of 0.1-0.6 mg/l. In each treatment up to 300 anthers were cultivated. Differences between treatments were evaluated using standard t-test. Studies have shown that in the anther culture of rapeseed on the tested nutrient media, morphogenic structures of different types (embryoids and callus) were originated. Synthetic auxin 2,4-D, regardless of the composition of the basic medium, caused the formation of structures of both types, though with a low frequency. Phytohormone BA of the cytokinin type had a similar effect. In this case, the frequency of structures was slightly higher, and the developed structures were represented mainly by embryoids. The joint action of cytokinin and auxin was the most favorable for the initiation of morphogenic structures. Such combination of phytohormones caused the formation of these structures with a frequency of 24.5-14.7% in the studied genotypes of winter rape. A similar effect of phytohormones on the induction and development of morphogenic structures was also observed in spring rape. In this case, a single basic MS medium was used. The experiment included treatments where phytohormones were absent (control), as well as various combinations of auxin and cytokinin. In the control treatment, the formation of new structures was not noted. In treatments with phytohormones, in addition to the medium with the combination of auxin and cytokinin, the medium in which only cytokinin was present was also rather effective. The treatment in which the action of auxin 2,4-D was combined with the action of another auxin, NAA, turned out to be practically ineffective. Thus, it was found that for the induction of morphogenic structures from microspores in rape anther culture of the tested genotypes, the combination of cytokinin with auxin, or the use of only single cytokinin BA without other phytohormones, had the most positive effect.


Crop Science ◽  
1964 ◽  
Vol 4 (1) ◽  
pp. 16-19 ◽  
Author(s):  
L. O. Hylton ◽  
D. E. Williams ◽  
A. Ultrich ◽  
D. R. Cornelius
Keyword(s):  

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