Development and Characterization of EST-SSR Markers from NCBI and cDNA Library in Cultivated Peanut (Arachis hypogaea L.)

Author(s):  
Yu Shanlin
2011 ◽  
Vol 38 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Yan Li ◽  
Charles Y. Chen ◽  
Steve J. Knapp ◽  
Albert K. Culbreath ◽  
C. Corley Holbrook ◽  
...  

Abstract A total of 709 SSR markers were collected from public databases and 556 SSRs passed an initial screen and were used to characterize 16 peanut (Arachis hypogaea) genotypes. PIC (polymorphism information content) scores and heterozygosity indices for each marker were calculated to assess the genetic diversity revealed by SSR markers and genetic distances were estimated from shared allele distances for construction of a cladogram by the Neighbor-Joining method to illustrate the genetic relationships among the genotypes. Two hundred thirty-five (42.27%) markers showed polymorphisms in these genotypes. The average heterozygosity estimated from these 556 SSRs was 0.225 with a range of 0 to 0.992 and the average PIC was 0.209. The average number of alleles per SSR was 2.5 with a range of 1 to 13. However, 410 SSR markers had only one allele, confirming that diversity of cultivated peanuts is very limited. Among the polymorphic SSR markers, 26.4% were dinucleotide GA repeat motif markers, followed by dinucleotide CT (10.4%), and trinucleotide TAA (9.6%). The dinucleotide and trinucleotide repeat motifs are the most abundant type of SSRs, and dinucleotide GA repeat motif shows a higher polymorphism in comparison to other types. The genetic relationships revealed from the cladogram are in agreement with the pedigrees and origins of the tested peanut genotypes, indicating that these SSR markers are useful tools for evaluation of genetic diversity in peanuts.


2010 ◽  
Vol 29 (2) ◽  
pp. 389-403 ◽  
Author(s):  
Xiaoping Chen ◽  
Ming Li Wang ◽  
Corley Holbrook ◽  
Albert Culbreath ◽  
Xuanqiang Liang ◽  
...  

2021 ◽  
Vol 13 (5) ◽  
pp. 2658
Author(s):  
Rose Nankya ◽  
John W. Mulumba ◽  
Hannington Lwandasa ◽  
Moses Matovu ◽  
Brian Isabirye ◽  
...  

The cultivated peanut (Arachis hypogaea L.) is one of the most widely consumed legumes globally due to its nutrient content, taste, and affordability. Nutrient composition and consumer preference were determined for twenty local farmer (landrace) and commercial peanut varieties grown in the Nakaseke and Nakasongola districts of the central wooded savanna of Uganda through sensory and laboratory evaluation. Significant differences in nutrient content (p < 0.05) among peanut varieties were found within and across sites. A significant relationship between nutrient content and consumer preference for varieties within and across sites was also realized (Wilk’s lambda = 0.05, p = 0.00). The differences in nutrient content influenced key organoleptic characteristics, including taste, crunchiness, appearance, and soup aroma, which contributed to why consumers may prefer certain varieties to others. Gender differences in variety selection were significantly related to consumer preference for the crunchiness of roasted peanut varieties (F = 5.7, p = 0.016). The results imply that selecting different varieties of peanuts enables consumers to receive different nutrient amounts, while experiencing variety uniqueness. The promotion of peanut intraspecific diversity is crucial for improved nutrition, organoleptic appreciation and the livelihood of those engaged in peanut value chains, especially for the actors who specialize in different peanut products. The conservation of peanut diversity will ensure that the present and future generations benefit from the nutritional content and organoleptic enjoyment that is linked to unique peanut varieties.


1988 ◽  
Vol 15 (2) ◽  
pp. 81-84 ◽  
Author(s):  
E. M. Ahmed ◽  
J. A. Applewhite

Abstract Florunner peanut seeds contained five trypsin isoinhibitors. Amino acid profiles of the trypsin inhibitors fraction showed high levels of aspartic acid, half-cystine and serine and low levels of histidine and tyrosine. The molecular weight of the inhibitor was 8.3 KDa. The presence of multiforms of this inhibitor, its low molecular weight and the high amount of half-cystine indicate that peanut trypsin inhibitor is of the Bowman-Birk type.


2013 ◽  
Vol 14 (1) ◽  
pp. 31 ◽  
Author(s):  
Imana L. Power ◽  
Albert K. Culbreath ◽  
Barry L. Tillman

Peanut rust, caused by Puccinia arachidis Speg, is an important foliar disease of peanut (Arachis hypogaea L.) in tropical countries. The best option for disease management is host resistance. The objectives of this project included characterizing peanut genotypes for resistance to P. arachidis, assessing the genetic variation of newly developed Collaborative Research and Support Program (CRSP) peanut breeding lines, and assessing genetic variability among P. arachidis populations. In field studies conducted over 2010-2011, several CRSP breeding lines demonstrated varying levels of rust resistance. Detached leaf assays were used to examine the components of resistance to P. arachidis. Few significant differences were observed in these studies. We used SSR markers to characterize newly developed CRSP breeding lines, plant introductions, and commonly grown cultivars. The SSR markers used detected polymorphisms but were not able to distinguish resistant from susceptible peanut genotypes. Sequences of the 5.8S-ITS2-28S region of P. arachidis isolates collected from different regions in the United States and other countries do not indicate high genetic variability among the populations. Accepted for publication 23 September 2013. Published 25 November 2013.


2011 ◽  
Vol 124 (4) ◽  
pp. 653-664 ◽  
Author(s):  
Hongde Qin ◽  
Suping Feng ◽  
Charles Chen ◽  
Yufang Guo ◽  
Steven Knapp ◽  
...  

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