Ultrasensitive determination for picomolar-level midecamycin in human serum by flow injection chemiluminescence using luminol–BSA system

A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL-1, giving the limit of detection (LOD) of 0.03 pg mL-1 (3σ). At a flow rate of 2.0 mL min-1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% (n=5). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.

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Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence

ISRN Analytical Chemistry ◽

10.5402/2012/809256 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5

Author(s):

Jiajia Wang ◽

Xijuan Tan ◽

Xin Li ◽

Jiangman Liu ◽

Zhenghua Song

Keyword(s):

Dissolved Oxygen ◽

Human Serum ◽

Flow Injection ◽

Inhibitory Effect ◽

Injection System ◽

Sampling Efficiency ◽

Relative Standard ◽

Flow Injection System ◽

Flow Injection Chemiluminescence

A sensitive chemiluminescence (CL) method for the determination of hesperetin at nanogram levels was first presented. It was based on the inhibitory effect of hesperetin on luminol-dissolved oxygen CL reaction in a flow injection system. The decrements of CL intensity were logarithm over the concentrations of hesperetin in a range of 0.5 to 1000 ng mL−1, with a detection limit of 0.2 ng mL−1 (3σ) and the relative standard deviations lower than 2.1%. At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 30 s, giving a sampling efficiency of 120 h−1. The proposed method was applied successfully to the direct hesperetin determination in human serum with recoveries from 97.0 to 106.6%, and indirect hesperidin quantification in 2.5 g pericarpium citri reticulatae showing that the content is 8.1±0.2%. The possible CL mechanism of luminol-dissolved oxygen-hesperetin reaction was also discussed in detail.

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Rapid assay of picogram level of sudan I in hot chilli sauce by flow injection chemiluminescence

Spectroscopy An International Journal ◽

10.1155/2007/704349 ◽

2007 ◽

Vol 21 (2) ◽

pp. 135-141 ◽

Cited By ~ 9

Author(s):

Xiaofei Gao ◽

Houyong Liu ◽

Zhenghua Song ◽

Xili He ◽

Faxin Dong

Keyword(s):

Standard Deviation ◽

Relative Standard Deviation ◽

Flow Rate ◽

Flow Injection ◽

Chemiluminescence Detection ◽

Chemiluminescence Method ◽

Chemiluminescence Intensity ◽

Relative Standard ◽

Sudan I ◽

Flow Injection Chemiluminescence

A novel chemiluminescence method for the assay of sudan I was designed using flow injection with chemiluminescence detection. The proposed method was based on the increment effect of sudan I on the chemiluminescence intensity in the luminol–KIO4system. The increment of chemiluminescence intensity was correlated with the sudan I concentration in the range from 0.1 to 10 pg ml−1, and the determination could be performed in 0.5 min in flow rate of 2 ml min−1, including sampling and washing, giving a throughput of 120 h−1with a relative standard deviation of less than 5.0%. The method had been successfully applied to the assay of sudan I in Pixian douban, Golden mark guilin chilli sauce and Golden mark satay sauce, and the recovery was 90.0–103.8%.

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Spectrophotometric flow injection procedure to indirect determination of paracetamol in pharmaceutical formulations using o-tolidine as reagent

Eclética Química Journal ◽

10.26850/1678-4618eqj.v33.2.2008.p47-53 ◽

2018 ◽

Vol 33 (2) ◽

pp. 47

Author(s):

Orlando Fatibello-Filho ◽

Heberth Juliano Vieira

Keyword(s):

Standard Deviation ◽

Detection Limit ◽

Flow Injection ◽

Pharmaceutical Formulations ◽

Injection Method ◽

Indirect Determination ◽

Analytical Curve ◽

Relative Standard ◽

Injection Procedure

A spectrophotometric flow injection method for the determination of paracetamol in pharmaceutical formulations is proposed. The procedure was based on the oxidation of paracetamol by sodium hypochloride and the determination of the excess of this oxidant using o-tolidine dichloride as chromogenic reagent at 430 nm. The analytical curve was linear in the paracetamol concentration range from 8.50 x 10-6 to 2.51 x 10-4 mol L-1 with a detection limit of 5.0 x 10-6 mol L-1. The relative standard deviation was smaller than 1.2% for 1.20 x 10-4 mol L-1 paracetamol solution (n = 10). The results obtained for paracetamol in pharmaceutical formulations using the proposed flow injection method and those obtained using a USP Pharmacopoeia method are in agreement at the 95% confidence level.

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Determination of Enoxacin in Tablet by Flow Injection Chemiluminescence

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.1880 ◽

2012 ◽

Vol 554-556 ◽

pp. 1880-1883 ◽

Cited By ~ 2

Author(s):

Nai Lin Ren ◽

Chi Yang

Keyword(s):

Standard Deviation ◽

Detection Limit ◽

Flow Injection ◽

Flow Injection Chemiluminescence

A new flow injection chemiluminescence (CL) method for the determination of Enoxacin（ENX）was described. The method was based on the weak CL reaction of Ce(Ⅳ)-SO32- sensitized by Pr3+- ENX. The relative CL intensity was linear with the concentration of ENX in the range of 3×10-8 g•mL-1~5.0×10-7 g•mL-1. The detection limit is 1.0×10-8 g•mL-1 .The rSuperscript textelative standard deviation (RSD) for the determination of 4.0×10-7 g•mL-1 ENX is 3.5% (n=11). The method has been successfully applied to the analysis of ENX in tablet

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Spectrophotometric flow injection procedure to indirect determination of paracetamol in pharmaceutical formulations using o-tolidine as reagent

Eclética Química ◽

10.1590/s0100-46702008000200007 ◽

2008 ◽

Vol 33 (2) ◽

pp. 47-54 ◽

Cited By ~ 3

Author(s):

O. Fatibello-Filho ◽

H. J. Vieira

Keyword(s):

Standard Deviation ◽

Detection Limit ◽

Flow Injection ◽

Pharmaceutical Formulations ◽

Injection Method ◽

Indirect Determination ◽

Analytical Curve ◽

Relative Standard ◽

Injection Procedure

A spectrophotometric flow injection method for the determination of paracetamol in pharmaceutical formulations is proposed. The procedure was based on the oxidation of paracetamol by sodium hypochloride and the determination of the excess of this oxidant using o-tolidine dichloride as chromogenic reagent at 430 nm. The analytical curve was linear in the paracetamol concentration range from 8.50 x 10-6 to 2.51 x 10-4 mol L-1 with a detection limit of 5.0 x 10-6 mol L-1. The relative standard deviation was smaller than 1.2% for 1.20 x 10-4 mol L-1 paracetamol solution (n = 10). The results obtained for paracetamol in pharmaceutical formulations using the proposed flow injection method and those obtained using a USP Pharmacopoeia method are in agreement at the 95% confidence level.

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Ultrasensitive determination of amoxicillin using chemiluminescence with flow injection analysis

Spectroscopy An International Journal ◽

10.1155/2006/270417 ◽

2006 ◽

Vol 20 (1) ◽

pp. 37-43 ◽

Cited By ~ 2

Author(s):

Xiaofeng Xie ◽

Zhenghua Song

Keyword(s):

Hydrogen Peroxide ◽

Standard Deviation ◽

Detection Limit ◽

Flow Injection ◽

Pharmaceutical Preparations ◽

Chemiluminescence Intensity ◽

Relative Standard ◽

Human Urine And Serum ◽

Urine And Serum

Results presented here reveal that amoxicillin can greatly enhance the chemiluminescence intensity generated from the reaction between luminol and hydrogen peroxide. The increment chemiluminescence signal was linearly dependent on amoxicillin concentration in the range from 10 pg·ml−1to 2 ng·ml−1(r2=0.9978) offering a detection limit as low as 3.5 pg·ml−1(3σ). At a flow rate of 2.0 ml·min−1, one analysis cycle, including sampling and washing, can be accomplished in 20 s with a relative standard deviation of less than 5%. The sensitive flow injection method was applied successfully to determine of amoxicillin in pharmaceutical preparations, human urine and serum without any pretreatment procedure, with recovery from 90.0% to 110.0% and relative standard deviations of less than 5.0%.

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Determination of Manganese in Feedstuffs by Flow Injection Spectrophotometry Based on Rapid Formation of Permanganate at Room Temperature

Journal of AOAC International ◽

10.1093/jaoac/84.4.1011 ◽

2001 ◽

Vol 84 (4) ◽

pp. 1011-1016 ◽

Cited By ~ 5

Author(s):

Jing-Fu Liu ◽

Ying-Di Feng ◽

Gui-Bin Jiang

Keyword(s):

Standard Deviation ◽

Detection Limit ◽

Flow Injection ◽

Room Temperature ◽

Sampling Rate ◽

Relative Standard ◽

Rapid Flow ◽

Rapid Formation ◽

Optimized Conditions

Abstract A simple and rapid flow injection spectrophotometric procedure was developed for determination of manganese. In the presence of pyrophosphate and acetate, manganese was immediately oxidized to permanganate by periodate at room temperature in slightly alkaline medium. Under optimized conditions, the determination was made with a sampling rate of 120/h, a linear range of 0–30 mg/L Mn(II), a detection limit (S/N = 3) of 0.08 mg/L, and a relative standard deviation of 0.6% (n = 11) at 10 mg/L Mn(II). The proposed method was used to determine manganese in trace mineral premixes and feedstuffs. Results agreed well with those obtained by the standard atomic absorption spectroscopy method.

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Determination of Prednisone Acetate at Trace Level by Flow Injection Analysis with Tris(1,10-Phenanthroline) Ruthenium(II) Chemiluminescence Detection

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.2729 ◽

2011 ◽

Vol 236-238 ◽

pp. 2729-2732

Author(s):

Pi Xue Gong ◽

Wei Hua Liu ◽

Wen Wen Liu ◽

Kang Du ◽

Kang Liu ◽

...

Keyword(s):

Standard Deviation ◽

Detection Limit ◽

Flow Injection ◽

Acidic Medium ◽

Luminescent Properties ◽

Chemiluminescence Detection ◽

Trace Level ◽

Experimental Conditions ◽

Relative Standard

A simple, sensitive and rapid method for flow injection with chemiluminescence analysis has been developed for the determination of prednisone acetate at trace level. This method is based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II)-potassium permanganate -prednisone acetate in acidic medium sensitized by Na2SO3. The optimized experimental conditions are evaluated. Under the optimum experimental conditions, the linear ranges of prednisone acetate are: 4.0×10-10~4.0×10-8g·mL-1and 4.0×10-8~2.0×10-7g·mL-1with a low detection limit is 4.5×10-11g·mL-1and the relative standard deviation for 1.0×10-8g·mL-1sample is 1.04% (n=11). The proposed method has been successfully applied to the determination of prednisone acetate in tablets.

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Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

Journal of Analytical Methods in Chemistry ◽

10.1155/2013/854041 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Jiajia Wang ◽

Xijuan Tan ◽

Zhenghua Song

Keyword(s):

Uric Acid ◽

Food Intake ◽

Human Serum ◽

Flow Injection ◽

Human Urine ◽

Inhibitory Effect ◽

System A ◽

Flow Injection Chemiluminescence ◽

Sensitive Method

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

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