scholarly journals Correlation between Biosurfactants and Antifungal Activity of a Biocontrol Bacterium, Bacillus amyloliquefaciens LM11

2017 ◽  
Vol 23 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Beom Ryong Kang ◽  
Yong Hwan Kim ◽  
Hyo Song Nam ◽  
Young Cheol Kim
2018 ◽  
Vol 21 (3) ◽  
pp. 153-162 ◽  
Author(s):  
Lei Tian ◽  
Shaohua Shi ◽  
Li Ji ◽  
Fahad Nasir ◽  
Lina Ma ◽  
...  

2012 ◽  
Vol 194 (24) ◽  
pp. 6934-6935 ◽  
Author(s):  
S.-Y. Lee ◽  
B.-Y. Kim ◽  
J.-H. Ahn ◽  
J. Song ◽  
Y.-J. Seol ◽  
...  

2009 ◽  
Vol 25 (4) ◽  
pp. 344-351 ◽  
Author(s):  
Kwang-Youll Lee ◽  
Kwang-Ryool Heo ◽  
Ki-Hyuck Choi ◽  
Hyun-Gi Kong ◽  
Jae-Sung Nam ◽  
...  

2020 ◽  
Vol 110 (2) ◽  
pp. 317-326 ◽  
Author(s):  
Ayaz Farzand ◽  
Anam Moosa ◽  
Muhammad Zubair ◽  
Abdur Rashid Khan ◽  
Muhammad Ayaz ◽  
...  

Sclerotinia sclerotiorum is a devastating necrotrophic pathogen that infects multiple crops, and its control is an unremitting challenge. In this work, we attempted to gain insights into the pivotal role of lipopeptides (LPs) in the antifungal activity of Bacillus amyloliquefaciens EZ1509. In a comparative study involving five Bacillus strains, B. amyloliquefaciens EZ1509 harboring four LPs biosynthetic genes (viz. surfactin, iturin, fengycin, and bacilysin) exhibited promising antifungal activity against S. sclerotiorum in a dual-culture assay. Our data demonstrated a remarkable upsurge in LPs biosynthetic gene expression through quantitative reverse transcription PCR during in vitro interaction assay with S. sclerotiorum. Maximum upregulation in LPs biosynthetic genes was observed on the second and third days of in vitro interaction, with iturin and fengycin being the highly expressed genes. Subsequently, Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry analysis confirmed the presence of LPs in the inhibition zone. Scanning electron microscope analysis showed disintegration, shrinkage, plasmolysis, and breakdown of fungal hyphae. During in planta evaluation, S. sclerotiorum previously challenged with EZ1509 showed significant suppression in pathogenicity on detached leaves of tobacco and rapeseed. The oxalic acid synthesis was also significantly reduced in S. sclerotiorum previously confronted with antagonistic bacterium. The expression of major virulence genes of S. sclerotiorum, including endopolygalacturonase-3, oxalic acid hydrolase, and endopolygalacturonase-6, was significantly downregulated during in vitro confrontation with EZ1509.


2018 ◽  
Vol 7 (18) ◽  
Author(s):  
Matthew J. Meier ◽  
Annette Dodge ◽  
Lee A. Beaudette

Bacillus amyloliquefaciens NRRL 942 is a Gram-positive bacterium with several potential industrial uses. We have sequenced the whole genome of this organism to assist in understanding the biological mechanisms that might modulate human health or environmental risk in the event of its release into the environment.


2020 ◽  
Vol 6 (4) ◽  
pp. 294
Author(s):  
Ezzeldin Ibrahim ◽  
Jinyan Luo ◽  
Temoor Ahmed ◽  
Wenge Wu ◽  
Chenqi Yan ◽  
...  

Biosynthesis of silver nanoparticles (AgNPs) using endophytic bacteria is a safe alternative to the traditional chemical method. The purpose of this research is to biosynthesize AgNPs using endophytic bacterium Bacillus endophyticus strain H3 isolated from onion. The biosynthesized AgNPs with sizes from 4.17 to 26.9 nm were confirmed and characterized by various physicochemical techniques such as Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV-visible spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) in addition to an energy dispersive spectrum (EDS) profile. The biosynthesized AgNPs at a concentration of 40 μg/mL had a strong antifungal activity against rice blast pathogen Magnaporthe oryzae with an inhibition rate of 88% in mycelial diameter. Moreover, the biosynthesized AgNPs significantly inhibited spore germination and appressorium formation of M. oryzae. Additionally, microscopic observation showed that mycelia morphology was swollen and abnormal when dealing with AgNPs. Overall, the current study revealed that AgNPs could protect rice plants against fungal infections.


2019 ◽  
Vol 203 ◽  
pp. 117-124 ◽  
Author(s):  
Lei Liu ◽  
Jing Liu ◽  
Xiaoting Liu ◽  
Chengwei Dai ◽  
Zexin Zhang ◽  
...  

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