scholarly journals Ocorrência de anticorpos contra Neospora caninum em bovinos e cães oriundo de propriedades produtoras de leite do Norte Central do Estado do Paraná

2018 ◽  
Vol 39 (6) ◽  
pp. 2449
Author(s):  
Simone Marins Spiti ◽  
Luiz Daniel de Barros ◽  
Mércia De Seixas ◽  
Ana Flavia Minutti ◽  
Thais Agostinho Martins ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Rare Event ◽  
Epidemiological Studies ◽  
Northern Region ◽  
Clot Retraction ◽  
Chain Reaction ◽  
Serum Samples ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain

Neosporosis is one of the principal causes of reproductive problems in cattle worldwide. The aim of this study was to evaluate the occurrence of anti-Neospora caninum antibodies in cattle and dogs from dairy farms from the central northern region of Paraná state. Blood samples with and without EDTA were collected from 400 cattle and 46 dogs from 20 properties. Nested polymerase chain reaction (n-PCR) was performed on whole blood samples and indirect immunofluorescence reaction (IFR) on the serum samples (after clot retraction). Cattle and dogs with titers ? 100 and ? 50, respectively, were considered positive. Anti-N. caninum was detected in 20,1% (80/400) of cattle, with titers ranging from 100 to 1600. The n-PCR presented only two cattle positives (0.5%). Anti-N. caninum was detected in 19,6% (9/46) of the dogs, with titers ranging from 50 to 6400. The occurrence of antibodies against N. caninum obtained in the present study was similar to those in studies performed in other regions of Paraná and Brazil. The probability of detecting parasitemia in epidemiological studies is a rare event.

scholarly journals Evaluation on the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats (Capra hircus) in Cebu, the Philippines

2019 ◽  
Vol 12 (6) ◽  
pp. 774-777 ◽  
Author(s):  
Adrian P. Ybañez ◽  
Orgil V. Arrabis ◽  
Dennis Justin M. Alvarez ◽  
Eloiza May S. Galon ◽  
Rhea Mae P. Jayag ◽  
...  
Keyword(s):  
Blood Smear ◽  
Peripheral Blood Smear ◽  
The Philippines ◽  
Capra Hircus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Blood Smear Examination ◽  
Polymerase Chain ◽  
Babesia Spp

Background: Tick-borne diseases are caused by a wide variety of viruses, pathogens, and diseases. Anaplasma, Ehrlichia, and Babesia spp. are among the most known tick-borne pathogens in Asia. In the Philippines, these pathogens were already reportedly present in dogs and large ruminants, but no study has been reported yet evaluating their presence in goats. Aim: The present study aimed to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. in goats in Cebu, the Philippines. Materials and Methods: A total of 100 blood samples from goats were collected in Cebu, the Philippines. Profile of sampled goats including age, body score, and sex was obtained. Peripheral blood smear examination and DNA extraction were performed. Nested polymerase chain reaction assay was used to evaluate the presence of Anaplasma, Ehrlichia, and Babesia spp. Results: None of the samples were found positive with Anaplasma, Ehrlichia, and Babesia spp. infection. Conclusion: Tested goats were negative with Anaplasma, Ehrlichia, and Babesia spp. and calls for continuous surveillance of these pathogens due to the reported detection of these pathogens in other livestock animals in the area.

scholarly journals Field evidence that roe deer (Capreolus capreolus) are a natural host for Ehrlichia phagocytophila

2000 ◽  
Vol 124 (2) ◽  
pp. 315-323 ◽  
Author(s):  
M. P. ALBERDI ◽  
A. R. WALKER ◽  
K. A. URQUHART
Keyword(s):  
Ixodes Ricinus ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Chain Reaction ◽  
Serum Samples ◽  
Blood Samples ◽  
Ehrlichia Phagocytophila ◽  
The United Kingdom ◽  
Field Evidence ◽  
Polymerase Chain

Samples of blood, spleen and legs from 112 culled roe deer (Capreolus capreolus) were collected from nine sites widespread in the United Kingdom. The prevalence of infection with Ehrlichia phagocytophila was determined by serology and polymerase chain reaction. Means of 58% of 102 plasma or serum samples were seroreactive by IFA, 38% of 84 blood samples and 29% of 82 spleen samples were positive by PCR. Ticks on legs of 71 roe deer were Ixodes ricinus larvae, nymphs and adults and 83% of legs were infested. Numbers of ticks corresponded positively to the percentage of samples positive for E. phagocytophila by serology and PCR for different sampling sites. Ixodes ricinus nymphs collected from the vegetation at one site with infected deer were analysed for infection with E. phagocytophila by examination of Feulgen stained salivary glands. Of 135 nymphs 5% were infected. These results confirm that roe deer are commonly parasitized by both E. phagocytophila and its vector tick in such a way that it is likely to be an important natural mammalian reservoir of E. phagocytophila.

Molecular Detection of Leptospira Species Serotypes in Iranian Stray Dogs

2019 ◽  
Author(s):  
Saam Torkan ◽  
Hassan Momtaz
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Serum Samples ◽  
Blood Samples ◽  
Stray Dogs ◽  
Wide Range ◽  
Leptospira Spp ◽  
Polymerase Chain ◽  
Leptospira Species

Background and Aims: Leptospirosis is a spirochetal disease with public health importance globally. This disease affects a wide range of domestic and wild animals. Dogs are one of the species most sensitive to Leptospira canicola and Leptospira icterohaemorrhagiae. The present study was concluded to evaluate the prevalence rate of Leptospira species and L. canicola and L. icterohaemorrhagiae serovars in Iranian stray dogs. Materials and Methods: One-hundred and twenty blood samples were first taken from stray dogs. Then the samples were transferred to the laboratory. Sera were extracted from blood samples and genomic DNA was extracted. DNA samples were subjected to conventional polymerase chain reaction. Positive samples for Leptospira spp. were analyzed for presence of L. canicola and L. icterohaemorrhagiaeserovars using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Nine samples out of 120 serum samples (7.5%) were positive for the flagella gene of the Leptospira spp. Prevalence of Leptospira spp. in serum samples of male and female dogs were 5.4% and 10.86%, respectively. Prevalence of L. canicola and L. icterohaemorrhagiae serovars were 55.55% and 33.33%, respectively. We found that 11.11% of samples were positive for both serovars. Two to three and 3-4 year old dogs had the highest prevalence of Leptospira spp. Conclusions: The considerable prevalence of leptospirta spp. and also their zoonotic serovars among Iranian stray dogs represented an important public health issue regarding the contact of healthy human with these dogs. Identification of infected dogs and their vaccination can inhibit the distribution of Leptospira spp.

Screening of toxoplasmosis in cancer patients: a concern

2018 ◽  
Vol 49 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Amir Abdoli ◽  
Mohammad Barati ◽  
Majid Pirestani ◽  
Abdolhossein Dalimi
Keyword(s):  
Infectious Disease ◽  
Polymerase Chain Reaction ◽  
Cancer Patients ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunocompromised Patients ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Different Types ◽  
Polymerase Chain

Toxoplasmosis is an opportunistic infectious disease in immunocompromised patients, including cancer patients, whose detection is by molecular and serological methods. A total of 106 blood samples from patients with different types of cancer were evaluated for anti- Toxoplasma gondii IgG and IgM antibodies by the enzyme-linked immunosorbent assay (ELISA) and the parasite DNA by nested polymerase chain reaction (PCR). These were detected in 41.51% (44/106) and 0.94% (1/106), respectively, but T. gondii IgM antibody was not detected at all. These results suggest that the screening of toxoplasmosis should be considered more routinely in cancer patients.

Use of a nested polymerase chain reaction (N-PCR) to detect Trypanosoma cruzi in blood samples from chronic chagasic patients and patients with doubtful serologies

2002 ◽  
Vol 43 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Gláucia Elisete Barbosa Marcon ◽  
Paula Durante Andrade ◽  
Dulcinéia Martins de Albuquerque ◽  
Jamiro da Silva Wanderley ◽  
Eros Antonio de Almeida ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain

scholarly journals Identification of Leptospira in Patients With Renal Failure Using Serological, Molecular and Pathological Based Techniques, in Shiraz, Iran

2022 ◽  
Author(s):  
Mostafa Sayyadi ◽  
Saeid Hosseinzadeh ◽  
Gholamreza Abdollahpour ◽  
Seyed Shahram Shekarforoush ◽  
Azadeh Samiei ◽  
...  
Keyword(s):  
Staining Method ◽  
Pathogenic Species ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Serum Samples ◽  
Biochemical Tests ◽  
Nested Polymerase Chain Reaction ◽  
Renal Disorders ◽  
Silver Staining Method ◽  
Polymerase Chain

Abstract Leptospirosis is a relatively rare bacterial infection that affects people and animals caused by pathogenic species of Leptospira. The present study was conducted using nested polymerase chain reaction (PCR), microscopic agglutination test (MAT) and hematological and biochemical tests on 200 blood samples of renal disorders patients in Shiraz, Iran. Also nested-PCR assay and Warthin-Starry (WS) silver staining method was performed on 30 nephrectomised kidney sample. The frequency of pathogenic species of Leptospira infection in patients with renal disorders was 20 % and this infection was significantly correlated with BUN, anemia, RDW, MCV, MCH and hemoglobin levels (P < 0.01). MAT analysis showed that serum samples had positive titers against L. Grippotyphosa (13 samples), L. Ballum (6 sample), L. Pomona (3 samples), L. Canicola (2 samples), L. Icterohaemorrhagiae (1 sample) and L. Hardjo (1 sample) serovars. Twenty-three percent of the kidney samples from the patients with pyelonephritis were infected with the pathogenic species of Leptospira. This study showed that pathogenic Leptospira serovars are present in this area and in patients with renal disorders more attention should be paid to this zoonotic disease.

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