scholarly journals Detection of buffaloes (Bubalus bubalis) seroreactive for vesicular stomatitis virus in the state of Paraíba, Northeastern Brazil

2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3769
Author(s):  
Camila de Sousa Bezerra ◽  
Jéssica Tatiane Sauthier ◽  
Juliana Felipetto Cargnelutti ◽  
Gisele Cândida Ramalho ◽  
Denise Batista Nogueira ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Virus Isolation ◽  
Neutralization Test ◽  
Bubalus Bubalis ◽  
The State ◽  
Virus Neutralization Test ◽  
Northeastern Brazil ◽  
Antibody Titers ◽  
Vesicular Stomatitis

This study aimed to determine the prevalence of vesicular stomatitis virus (VSV) in buffaloes in the state of Paraíba, Northeastern Brazil. The study was carried out in 14 herds in the municipalities of Alagoa Nova, Areia, Campina Grande, Guarabira, Juripiranga, Santa Helena, Sapê, Rio Tinto, Santana dos Garrotes, Itatuba, Solânea, and Cacimbas. The studied population was formed by buffalo females reared for meat and milk, comprising of mixed and Murrah breeds, at least 24 months of age. For the serological diagnosis of VSIV-3, the virus-neutralization test (VN) was performed, using the VSIV-3 isolate 2013 São Bento/Paraíba. Real prevalence was 2.6% for VSIV-3, with antibody titers ranging from 160 to 1280. This is the first study to characterize VSV circulation in the buffalo population in Northeastern Brazil, where infection is considered endemic; some aspects of virus maintenance are not fully understood, such as the role of reservoirs in endemic areas. The identification of seroreactive animals in this study demonstrates the circulation of VSIV-3 in the buffalo species. Reports of virus isolation in this species have not yet been described, which suggests the need for investigating the role of buffaloes in vesicular stomatitis epidemiology.

Role of matrix protein in cytopathogenesis of vesicular stomatitis virus.

1990 ◽  
Vol 64 (4) ◽  
pp. 1716-1725 ◽  
Author(s):  
D Blondel ◽  
G G Harmison ◽  
M Schubert
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Matrix Protein ◽  
Vesicular Stomatitis

scholarly journals Valosin-Containing Protein (VCP/p97) Is a Potential Antiviral Target against Mononegavirales

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 108
Author(s):  
Victor Latorre ◽  
Ron Geller
Keyword(s):  
Life Cycle ◽  
Respiratory Syncytial Virus ◽  
Vesicular Stomatitis Virus ◽  
Molecular Chaperones ◽  
Cellular Machinery ◽  
Vesicular Stomatitis ◽  
Medical Importance ◽  
Syncytial Virus ◽  
Chaperone Network

The viral order Mononegavirales consist of eight virus families. Members of these families include some of the most infectious (Measles, lethal (Ebola and Rabies), and most common viruses (Respiratory syncytial virus, RSV). Despite their medical importance, few vaccines and no antiviral treatments are available for treating infections with these viruses. Being obligate cellular parasites, viruses must rely on the cellular machinery for their replication. One example of this is the widespread use of molecular chaperones, which assist the correct folding of newly synthesized proteins, refold misfolded or aggregated proteins, and play key roles in maintaining proteostasis in cells. Targeting chaperones required for viral replication may, therefore, provide an antiviral approach. In this work, we set out to identify all the members of the cytoplasmic chaperone network that are involved in the replication of RSV using an RNA interference screen. Among our hits is valosin-containing protein (VCP; also known as p97), a chaperone involved in ubiquitin-mediated protein degradation, which has been shown to play a role in the life cycle of several viruses. We investigated the role of VCP during RSV and vesicular stomatitis virus (VSV) infections using specific VCP inhibitors. Our results suggest that VCP activity is necessary for RSV and VSV replication and may constitute a promising antiviral approach for the Mononegavirales.

scholarly journals Rapid Quantification of SARS-CoV-2-Neutralizing Antibodies Using Propagation-Defective Vesicular Stomatitis Virus Pseudotypes

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 386 ◽  
Author(s):  
Ferdinand Zettl ◽  
Toni Luise Meister ◽  
Tanja Vollmer ◽  
Bastian Fischer ◽  
Jörg Steinmann ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Pearson Correlation ◽  
Spike Protein ◽  
Virus Neutralization ◽  
Pseudotype Virus ◽  
Vesicular Stomatitis ◽  
Level 1 ◽  
Biosafety Level

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2, a new member of the genus Betacoronavirus, is a pandemic virus, which has caused numerous fatalities, particularly in the elderly and persons with underlying morbidities. At present, there are no approved vaccines nor antiviral therapies available. The detection and quantification of SARS-CoV-2-neutralizing antibodies plays a crucial role in the assessment of the immune status of convalescent COVID-19 patients, evaluation of recombinant therapeutic antibodies, and the evaluation of novel vaccines. To detect SARS-CoV-2-neutralizing antibodies, classically, a virus-neutralization test has to be performed at biosafety level 3, considerably limiting the general use of this test. In the present work, a biosafety level 1 pseudotype virus assay based on a propagation-incompetent vesicular stomatitis virus (VSV) has been used to determine the neutralizing antibody titers in convalescent COVID-19 patients. The neutralization titers in serum of two independently analyzed patient cohorts were available within 18 h and correlated well with those obtained with a classical SARS-CoV-2 neutralization test (Pearson correlation coefficients of r = 0.929 and r = 0.939, respectively). Most convalescent COVID-19 patients had only low titers of neutralizing antibodies (ND50 < 320). The sera of convalescent COVID-19 patients also neutralized pseudotype virus displaying the SARS-CoV-1 spike protein on their surface, which is homologous to the SARS-CoV-2 spike protein. In summary, we report a robust virus-neutralization assay, which can be used at low biosafety level 1 to rapidly quantify SARS-CoV-2-neutralizing antibodies in convalescent COVID-19 patients and vaccinated individuals.

scholarly journals Rab8 promotes polarized membrane transport through reorganization of actin and microtubules in fibroblasts.

1996 ◽  
Vol 135 (1) ◽  
pp. 153-167 ◽  
Author(s):  
J Peränen ◽  
P Auvinen ◽  
H Virta ◽  
R Wepf ◽  
K Simons
Keyword(s):  
Epithelial Cells ◽  
Membrane Transport ◽  
Vesicular Stomatitis Virus ◽  
Actin Filaments ◽  
Mdck Cells ◽  
Basolateral Membrane ◽  
Wild Type ◽  
Expression Systems ◽  
Vesicular Stomatitis

Rab8 is a small Ras-like GTPase that regulates polarized membrane transport to the basolateral membrane in epithelial cells and to the dendrites in neurons. It has recently been demonstrated that fibroblasts sort newly synthesized proteins into two different pathways for delivery to the cell surface that are equivalent to the apical and the basolateral post-Golgi routes in epithelial cells (Yoshimori, T., P. Keller, M.G. Roth, and K. Simons. 1996. J. Cell Biol. 133:247-256). To determine the role of Rab8 in fibroblasts, we used both transient expression systems and stable cell lines expressing mutant or wild-type (wt) Rab8. A dramatic change in cell morphology occurred in BHK cells expressing both the wt Rab8 and the activated form of the GTPase, the Rab8Q67L mutant. These cells formed processes as a result of a reorganization of both their actin filaments and microtubules. Newly synthesized vesicular stomatitis virus G glycoprotein, a basolateral marker protein in MDCK cells, was preferentially delivered into these cell outgrowths. Based on these findings, we propose that Rab8 provides a link between the machinery responsible for the formation of cell protrusions and polarized biosynthetic membrane traffic.

scholarly journals Role of non-raft cholesterol in lymphocytic choriomeningitis virus infection via α-dystroglycan

2006 ◽  
Vol 87 (3) ◽  
pp. 673-678 ◽  
Author(s):  
Waris A. Shah ◽  
Huashan Peng ◽  
Salvatore Carbonetto
Keyword(s):  
Extracellular Matrix ◽  
Lipid Rafts ◽  
Vesicular Stomatitis Virus ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Ganglioside Gm1 ◽  
The Family ◽  
Lymphocytic Choriomeningitis Virus Infection ◽  
Vesicular Stomatitis

Dystroglycan (DG) is an extracellular matrix receptor necessary for the development of metazoans from flies to humans and is also an entry route for various pathogens. Lymphocytic choriomeningitis virus (LCMV), a member of the family Arenaviridae, infects by binding to α-DG. Here, the role of cholesterol lipid rafts in infection by LCMV via α-DG was investigated. The cholesterol-sequestering drugs methyl-β-cyclodextrin (MβCD), filipin and nystatin inhibited the infectivity of LCMV selectively, but did not affect infection by vesicular stomatitis virus. Cholesterol loading after depletion with MβCD restored infectivity to control levels. DG was not found in lipid rafts identified with the raft marker ganglioside GM1. Treatment with MβCD, however, enhanced the solubility of DG. This may reflect the association of DG with cholesterol outside lipid rafts and suggests that association of DG with non-raft cholesterol is critical for infection by LCMV through α-DG.

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