scholarly journals First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil

2020 ◽  
Vol 41 (4) ◽  
pp. 1259
Author(s):  
Alessandra Taroda ◽  
Luiz Daniel de Barros ◽  
Mércia de Seixas ◽  
Sérgio Tosi Cardim ◽  
João Pedro Sasse ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Mitochondrial Genome ◽  
Cytochrome C Oxidase ◽  
Dna Extraction ◽  
Nested Pcr ◽  
Molecular Detection ◽  
Southern Brazil ◽  
Oxidase Gene ◽  
Fecal Samples ◽  
Eimeria Spp

The aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect the DNA presence of this coccidian in eared dove feces. From 214 birds, 171 (79.9%) were positive for Eimeria spp. There was significantly difference of positivity between the site of capture. When analyzing the genders, the numbers of positive males were 84/103 (81.5%) and females 87/111 (78.4%). To the best of the authors’ knowledge, this is the first study with molecular prevalence of Eimeria sp. in Z. auriculata. Further studies should be done to identify the species of Eimeria that infect eared doves Z. auriculata.

scholarly journals DNA extraction methods for molecular detection of Eimeria spp. in cattle and sheep

2020 ◽  
Vol 40 (7) ◽  
pp. 514-518
Author(s):  
Caroline Z. Reginato ◽  
Patrícia Bräunig ◽  
Luiza P. Portella ◽  
Ana Paula G. Mortari ◽  
Camila E. Minuzzi ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Detection ◽  
Dna Detection ◽  
Eimeria Species ◽  
Extraction Methods ◽  
Ammonium Bromide ◽  
Oocyst Wall ◽  
Fecal Samples ◽  
Eimeria Spp ◽  
Commercial Kit

ABSTRACT: Molecular detection of Eimeria species in fecal samples can be useful for experimental and diagnostic purposes. However, the parasite quantity presence in feces and the oocyst wall are an obstacle in DNA extraction protocols. Therefore, adequate sampling and effective disruption of the oocysts are essential to improve the accuracy of DNA detection by PCR. The aims of this study were to evaluate the suitability of six protocols for DNA extraction from Eimeria spp. present in bovine and sheep. Twenty pools of fecal samples from cattle (10 pools) and sheep (10 pools) were distributed to six DNA extraction protocols: commercial kit, commercial kit with modification, DNAzol, cetyl-trimethyl ammonium bromide (CTAB), glass beads and commercial kit for fecal samples. Fecal samples were submitted to DNA extraction and PCR. Among the protocols tested, CTAB was determined to be most suitable for DNA extraction from oocysts (90% of DNA detection by PCR); DNAzol and CTAB resulted in higher DNA detection from bovine samples (80%). CTAB and commercial kit with modification improved PCR detection of Eimeria spp. in sheep samples, with positive amplification of DNA in all tested samples.

scholarly journals Co-ordinate decrease in the expression of the mitochondrial genome and nuclear genes for mitochondrial proteins in the lactation-induced mitochondrial hypotrophy of rat brown fat

1995 ◽  
Vol 308 (3) ◽  
pp. 749-752 ◽  
Author(s):  
I Martin ◽  
M Giralt ◽  
O Viñas ◽  
R Iglesias ◽  
T Mampel ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Mitochondrial Genome ◽  
Cytochrome C Oxidase ◽  
Molecular Mechanisms ◽  
Uncoupling Protein ◽  
Relative Content ◽  
Nuclear Genes ◽  
Mitochondrial Proteins ◽  
Mrna Levels ◽  
Brown Adipose

The relative abundance of the mitochondrial-encoded mRNAs for cytochrome c oxidase subunit II and NADH dehydrogenase subunit I was lower in brown adipose tissue (BAT) from lactating rats than in virgin controls. This decrease was in parallel with a significant decrease in mitochondrial 16 S rRNA levels and in the relative content of mitochondrial DNA in the tissue. BAT from lactating rats showed lowered mRNA expression of the nuclear-encoded genes for the mitochondrial uncoupling protein, subunit IV of cytochrome c oxidase and the adenine nucleotide translocase isoforms ANT1 and ANT2, whereas mRNA levels for the ATP synthase beta-subunit were unchanged. However, the relative content of this last protein was lower in BAT mitochondria from lactating rats than in virgin controls. It is concluded that lactation-induced mitochondrial hypotrophy in BAT is associated with a co-ordinate decrease in the expression of the mitochondrial genome and nuclear genes for mitochondrial proteins. This decrease is caused by regulatory events acting at different levels, including pre- and post-transcriptional regulation. BAT appears to be a useful model with which to investigate the molecular mechanisms involved in the co-ordination of the expression of the mitochondrial and nuclear genomes during mitochondrial biogenesis.

scholarly journals Novel Point Mutations and A8027G Polymorphism in Mitochondrial-DNA-Encoded Cytochrome c Oxidase II Gene in Mexican Patients with Probable Alzheimer Disease

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Verónica Loera-Castañeda ◽  
Lucila Sandoval-Ramírez ◽  
Fermín Paul Pacheco Moisés ◽  
Miguel Ángel Macías-Islas ◽  
Moisés Alejandro Alatorre Jiménez ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Alzheimer Disease ◽  
Cytochrome C Oxidase ◽  
Point Mutations ◽  
Mitochondrial Cytochrome ◽  
Catalytic Core ◽  
Oxidase Gene ◽  
Mexican Patients ◽  
Mitochondrial Cytochrome C

Mitochondrial dysfunction has been thought to contribute to Alzheimer disease (AD) pathogenesis through the accumulation of mitochondrial DNA mutations and net production of reactive oxygen species (ROS). Mitochondrial cytochrome c-oxidase plays a key role in the regulation of aerobic production of energy and is composed of 13 subunits. The 3 largest subunits (I, II, and III) forming the catalytic core are encoded by mitochondrial DNA. The aim of this work was to look for mutations in mitochondrial cytochrome c-oxidase gene II (MTCO II) in blood samples from probable AD Mexican patients.MTCO IIgene was sequenced in 33 patients with diagnosis of probable AD. Four patients (12%) harbored the A8027G polymorphism and three of them were early onset (EO) AD cases with familial history of the disease. In addition, other four patients with EOAD had only one of the following point mutations: A8003C, T8082C, C8201T, or G7603A. Neither of the point mutations found in this work has been described previously for AD patients, and the A8027G polymorphism has been described previously; however, it hasn’t been related to AD. We will need further investigation to demonstrate the role of the point mutations of mitochondrial DNA in the pathogenesis of AD.

Novel DNA Barcode Sequence Discovery from Transcriptome of Acheta domesticus: A Partial Mitochondrial DNA

2019 ◽  
Vol 967 ◽  
pp. 59-64
Author(s):  
Yash Munnalal Gupta ◽  
Kittisak Buddhachat ◽  
Surin Peyachoknagul ◽  
Somjit Homchan
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Mitochondrial Genome ◽  
Cytochrome C Oxidase ◽  
Acheta Domesticus ◽  
Reference Sequence ◽  
Rna Seq ◽  
House Cricket ◽  
Oxidase Subunit ◽  
Mtdna Sequence

The potential of mitochondrial DNA (mtDNA) genes are well-known for species identification and to establish a phylogenetic relationship. The De-novo transcriptome assembly of Acheta domesticus commonly known as house cricket, is provides important segments of DNA fragments from mitochondrial DNA due to higher abundance of its mRNA. When the reference sequence with gene annotation is absent for assembling and aligning desire gene sequences, like in the present case, the most similar sequence is obtained from online insect mitochondrial genome database to find mitochondrial DNA conserved domains of interested gene from high throughput RNA sequencing (RNA-seq) data. The RNA-seq data of Acheta domesticus transcriptome is used to retrieve single nucleotide fragment out of 50,046 assembled contigs to discover three important genes from mtDNA of the house cricket. Present study provides effective workflow to identify genes like cytochrome c oxidase subunit II (COX2), NADH dehydrogenase subunit 2 (ND2), cytochrome c oxidase subunit I (COX1) from mtDNA in large sequence archive of RNA-seq data. These three novel barcode sequences will be useful for genetic identification and evolution investigation of Acheta domesticus. The partial mtDNA sequence with these genes will be important for mitochondrial genome construction.

scholarly journals Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Camila Serina Lasta ◽  
Andrea Pires dos Santos ◽  
Joanne Belle Messick ◽  
Simone Tostes Oliveira ◽  
Alexander Welker Biondo ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Chronic Infection ◽  
Molecular Detection ◽  
Hematological Parameters ◽  
Southern Brazil ◽  
Anaplasma Platys ◽  
Porto Alegre ◽  
Serum Samples ◽  
Hematological Abnormalities ◽  
Dot Elisa

The aims of this study were to determine the occurrence ofAnaplasma platys and Ehrlichia canisinfection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasmaspp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platysand E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive forAnaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canisantibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre.

Mitochondrial genome rearrangement leads to extension and relocation of the cytochrome c oxidase subunit I gene in sorghum

Cell ◽  
1986 ◽  
Vol 47 (4) ◽  
pp. 567-576 ◽  
Author(s):  
Julia Bailey-Serres ◽  
Deborah K. Hanson ◽  
Thomas D. Fox ◽  
Christopher J. Leaver
Keyword(s):  
Cytochrome C ◽  
Mitochondrial Genome ◽  
Cytochrome C Oxidase ◽  
Genome Rearrangement ◽  
Oxidase Subunit ◽  
I Gene
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