Molecular detection of Ehrlichia canisand Anaplasma platys in dogs in Southern Brazil

The aims of this study were to determine the occurrence ofAnaplasma platys and Ehrlichia canisinfection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasmaspp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platysand E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive forAnaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canisantibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre.

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First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n4p1259 ◽

2020 ◽

Vol 41 (4) ◽

pp. 1259

Author(s):

Alessandra Taroda ◽

Luiz Daniel de Barros ◽

Mércia de Seixas ◽

Sérgio Tosi Cardim ◽

João Pedro Sasse ◽

...

Keyword(s):

Cytochrome C ◽

Mitochondrial Genome ◽

Cytochrome C Oxidase ◽

Dna Extraction ◽

Nested Pcr ◽

Molecular Detection ◽

Southern Brazil ◽

Oxidase Gene ◽

Fecal Samples ◽

Eimeria Spp

The aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect the DNA presence of this coccidian in eared dove feces. From 214 birds, 171 (79.9%) were positive for Eimeria spp. There was significantly difference of positivity between the site of capture. When analyzing the genders, the numbers of positive males were 84/103 (81.5%) and females 87/111 (78.4%). To the best of the authors’ knowledge, this is the first study with molecular prevalence of Eimeria sp. in Z. auriculata. Further studies should be done to identify the species of Eimeria that infect eared doves Z. auriculata.

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Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

Ciência Rural ◽

10.1590/0103-8478cr20190414 ◽

2019 ◽

Vol 49 (12) ◽

Author(s):

Verucia Maria Dias Brandão ◽

Pedro Henrique Marques Barrozo ◽

Luciane Oeiras Sousa ◽

Rafaelle Cunha dos Santos ◽

Katiane Schwanke ◽

...

Keyword(s):

Nested Pcr ◽

Molecular Detection ◽

Ehrlichia Canis ◽

Anaplasma Platys ◽

Blood Samples ◽

Stray Dogs ◽

Significant Difference ◽

The City

ABSTRACT: The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

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Molecular Detection of Trypanosoma kaiowa in Tabanus triangulum (Diptera: Tabanidae) from the Coastal Plain of Rio Grande do Sul, Southern Brazil

Acta Parasitologica ◽

10.1007/s11686-021-00440-1 ◽

2021 ◽

Author(s):

Gratchela D. Rodrigues ◽

Eduardo Blodorn ◽

Ândrio Zafalon-Silva ◽

William Domingues ◽

Roberta Marques ◽

...

Keyword(s):

Coastal Plain ◽

Molecular Detection ◽

Rio Grande ◽

Southern Brazil ◽

Rio Grande Do Sul

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Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651992000100010 ◽

1992 ◽

Vol 34 (1) ◽

pp. 55-60 ◽

Cited By ~ 34

Author(s):

Eide Dias Camargo ◽

Paulo Mutuko Nakamura ◽

Adelaide José Vaz ◽

Marcos Vinícius da Silva ◽

Pedro Paulo Chieffi ◽

...

Keyword(s):

Low Cost ◽

Clinical Signs ◽

Toxocara Canis ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Specific Antibodies ◽

Normal Individuals ◽

Before And After ◽

Dot Elisa ◽

Stability Time

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

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Molecular detection of Anaplasma platys infection in free-roaming dogs and ticks from Kenya and Ivory Coast

Parasites & Vectors ◽

10.1186/s13071-016-1443-3 ◽

2016 ◽

Vol 9 (1) ◽

Cited By ~ 17

Author(s):

Ioana Adriana Matei ◽

Gianluca D’Amico ◽

Patrick K. Yao ◽

Angela Monica Ionică ◽

Paul W. N. Kanyari ◽

...

Keyword(s):

Ivory Coast ◽

Molecular Detection ◽

Anaplasma Platys

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Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-296120180069 ◽

2018 ◽

Vol 27 (4) ◽

pp. 505-513 ◽

Cited By ~ 2

Author(s):

Anna Cláudia Baumel Mongruel ◽

Priscila Ikeda ◽

Keyla Carstens Marques de Sousa ◽

Jyan Lucas Benevenute ◽

Margarete Kimie Falbo ◽

...

Keyword(s):

18S Rrna ◽

Molecular Detection ◽

Phylogenetic Analyses ◽

Southern Brazil ◽

Conventional Pcr ◽

Pathogenic Potential ◽

Vector Borne ◽

Pcr Assays ◽

Etiological Agents ◽

Positive Results

Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.

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Hematological abnormalities in HIV infected individuals in correlation to CD4 counts and ART status

Asian Journal of Medical Sciences ◽

10.3126/ajms.v7i4.14033 ◽

2016 ◽

Vol 7 (4) ◽

pp. 14-18 ◽

Cited By ~ 1

Author(s):

R Raman Thulasi ◽

D Manimaran ◽

G Hemanathan ◽

Tameem Afroz ◽

Radha Sagar

Keyword(s):

Opportunistic Infections ◽

Hematological Parameters ◽

Cd4 Count ◽

Health Concern ◽

Medical Sciences ◽

Cd4 Counts ◽

Medical Centers ◽

Hematological Abnormalities ◽

Set Up ◽

One Year

Background: HIV is pandemic and remains as a public health concern for many decades. This infection though associated with many opportunistic infections and neoplasms, it is further complicated with marked hematological abnormalities. The aim of this study is to determine the magnitude & severity of hematological abnormalities in HIV infected individuals and also to analyze these abnormalities in correlation with the CD4 counts. We also compared these hematological abnormalities in patients on ART and those not on ART.Materials and Methods: The study was conducted for a period of one year, on 120 HIV positive cases including both patients on ART & not on ART. Controls with similar age and sex distribution was set up. The blood samples were collected and processed in an automated cell counter. The parameters were tabulated and analyzed with respect to CD4 count & ART status.Results: Among the total of 120 HIV cases, 77% had anemia, 21% had leucopenia and 5% had thrombocytopenia. The magnitude and severity of anemia, leucopenia, thrombocytopenia and other parameters was found to be more in patients not on ART, when compared to patients on ART. Similarly, the magnitude and severity of most of hematological abnormalities were inversely proportional to the CD4 count in non-ART cases but not with cases on ART.Conclusion: The basic hematological parameters can be used as a prospective screening test to assess the severity and progression of HIV infection when CD4 count is not available. These parameters can also be used to assess the response to anti-retroviral treatment. Therefore, these basic hematological investigations readily available at all medical centers are of great use while treating HIV infected patients.Asian Journal of Medical Sciences Vol.7(4) 2016 14-18

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Appropriation and rationality in hip hop groups organization practices in Porto Alegre: an analysis on the perspective of Guerreiro Ramos

Cadernos EBAPE BR ◽

10.1590/s1679-39512010000200003 ◽

2010 ◽

Vol 8 (2) ◽

pp. 209-225

Author(s):

Guilherme Dornelas Camara ◽

Sueli Goulart ◽

Rafaela Mendes Reinher

Keyword(s):

Cultural Study ◽

Social Action ◽

Hip Hop ◽

Organizational Practices ◽

Southern Brazil ◽

Cultural Artifacts ◽

Porto Alegre ◽

Foreign Experience ◽

New Science ◽

Economic Criteria

This paper discusses the organizational practices of hip hop groups from Porto Alegre, southern Brazil, as they appropriate foreign experience and rationally guide their social action. The aim of this work is to investigate these practices, based on the formulations of Brazilian sociologist Alberto Guerreiro Ramos, as they are enunciated in the works The Sociological Reduction and The New Science of Organizations. This approach offers fruitful material to broaden the theory and may be subsidiary to this kind of socio-cultural study, explaining why it is pertinent in this field. In this sense, the hip hop movement can be viewed as valuable when it comes to understanding phenomena which, due to (and in spite of) being originally transnational, or even "foreign", act or interact worldwide. Examining the organization practices and social action of these groups also helped identify a concern with the production of cultural artifacts that represent and form a symbolism that is historically and culturally located. Another issue that was broached concerns the market since for those groups it is not a category absent from their struggles and actions, but the economic criteria are incidental in relation to their members' motivation.

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Efeitos de diferentes doses de hiclato de doxiciclina nos parâmetros hematológicos de cães com erliquiose

Semina Ciências Biológicas e da Saúde ◽

10.5433/1679-0367.2016v37n1p53 ◽

2016 ◽

Vol 37 (1) ◽

pp. 53

Author(s):

Mariana Cristina Hoeppner Rondelli ◽

Reinaldo Juan Garrido Palacios Junior ◽

Igor Luiz Salardani Senhorello ◽

Áureo Evangelista Santana ◽

Mirela Tinucci-Costa

Keyword(s):

Nested Pcr ◽

Ehrlichia Canis ◽

Babesia Canis ◽

Anaplasma Platys ◽

Leptospira Spp

Resumo: Este estudo objetivou comparar os efeitos de duas doses de hiclato de doxiciclina sobre a contagem de hemácias, concentração de hemoglobina, hematócrito, contagem de leucócitos e plaquetas de cães com erliquiose. Foram estudados os grupos I, composto por cães saudáveis (n=6), negativos na sorologia para Ehrlichia canis e Leptospira spp., na PCR em tempo real para E. canis e Anaplasma platys e na semi–nested PCR para Babesia canis; grupos II (n=6) e III (n=6), compostos por cães com história clínica sugestiva, sorologia e/ou PCR em tempo real positivas para E. canis, e negativos na pesquisa de anticorpos anti-Leptospira spp., na PCR em tempo real para A. platys e na semi–nested PCR para B. canis. Os cães doentes foram tratados com hiclato de doxiciclina via oral a cada 12 horas por 30 dias (5 mg/kg, grupo II; 10 mg/kg, grupo III). Hemogramas foram feitos antes, decorridos 15 dias e aos 10 dias após o término dos tratamentos. Não houve diferenças entre os grupos nos tempos avaliados quanto às variáveis hemácias, hemoglobina, hematócrito e leucócitos. Observou-se diferença quanto aos trombócitos entre o grupo I e os grupos II e III (p<0,0001) no início do estudo. Aos 15 dias de tratamento, a média do grupo III foi menor que dos grupos I (p=0,008) e II (p=0,0007), indicando persistência da trombocitopenia, ausente no grupo II. Não houve diferença entre os grupos no tempo final, sugerindo que ambos os tratamentos promoveram aumento da contagem de plaquetas em cães naturalmente infectados por E. canis.

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